LOCUS BBa_K156022 959 bp DNA linear 31-Oct-2024 DEFINITION Promoter + RBS + phaB1 + double terminator ACCESSION BBa_K156022 VERSION BBa_K156022.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 RBS 63..75 /label=RBS-3\Weak RBS 69..72 /label=RBS misc_feature 63..75 /label=BBa_B0032 RBS 63..75 /label=BBa_B0032 CDS 82..822 /label=BBa_K156013 stem_loop 842..885 /label=stem_loop misc_feature 831..910 /label=BBa_B0010 terminator 831..910 /label=BBa_B0010 stem_loop 926..945 /label=T7 TE misc_feature 952..952 /label=stop polyA_site 946..959 /label=polya misc_feature 919..959 /label=BBa_B0012 terminator 919..959 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agtcacacag gaaagtacta gatgactcaa cgtatcgcat atgtaactgg tggtatgggt 121 ggtatcggta ctgcaatttg ccagcgtctg gcgaaagacg gtttccgtgt tgttgcgggc 181 tgcggtccga actccccgcg tcgtgaaaag tggctggaac aacagaaagc cctgggcttc 241 gacttcattg cctccgaggg taatgtagct gactgggatt ccaccaagac tgccttcgat 301 aaagttaaat ctgaagtggg cgaagtagat gtactgatca acaacgccgg tattactcgt 361 gatgtcgtat tccgcaaaat gacccgtgca gactgggatg cagttatcga caccaacctg 421 acgtctctgt tcaacgttac caaacaggtt attgatggta tggctgaccg tggctggggc 481 cgcatcgtga acatctctag cgttaacggc caaaaaggcc aatttggtca gacgaattac 541 agcacggcta aagcaggcct gcacggtttc accatggcac tggcgcagga agtggcgacc 601 aaaggtgtta ccgttaatac cgtttctcca ggttacatcg ccaccgatat ggttaaggct 661 atccgccaag atgttctgga caagatcgtg gctaccattc cggttaaacg cctgggcctg 721 ccggaagaaa ttgcgtccat ctgtgcgtgg ctgagctccg aagagtctgg tttttccacc 781 ggtgcggatt tctctctgaa cggtggtctg cacatgggtt gatactagag ccaggcatca 841 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 901 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //