LOCUS       BBa_K156023             1567 bp    DNA     linear       22-Dec-2024
DEFINITION  Promoter + RBS + phaC1 + double terminator
ACCESSION   BBa_K156023
VERSION     BBa_K156023.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..75
                     /label=RBS-3\Weak
     RBS             69..72
                     /label=RBS
     misc_feature    63..75
                     /label=BBa_B0032
     RBS             63..75
                     /label=BBa_B0032
     CDS             82..1430
                     /label=BBa_K156014
     stem_loop       1450..1493
                     /label=stem_loop
     misc_feature    1439..1518
                     /label=BBa_B0010
     terminator      1439..1518
                     /label=BBa_B0010
     stem_loop       1534..1553
                     /label=T7 TE
     misc_feature    1560..1560
                     /label=stop
     polyA_site      1554..1567
                     /label=polya
     misc_feature    1527..1567
                     /label=BBa_B0012
     terminator      1527..1567
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaagtacta gatggcaact ggtaaaggtg ctgctgcatc tactcaagaa
      121 ggtaaatccc agccgttcaa agtgacccct ggtccgttcg atccggcgac ctggctggaa
      181 tggagccgcc agtggcaggg cactgaaggc aacggccacg cagctgcttc tggcattccg
      241 ggtctggacg ctctggcggg cgtaaaaatc gcaccggcac aactgggcga catccagcaa
      301 cgttatatga aagatttcag cgcgctgtgg caggcgatgg cggaaggcaa agccgaagcg
      361 accggtccgc tgcacgaccg tcgtttcgca ggtgacgcgt ggcgtaccaa cctgccatac
      421 cgtttcgctg ccgcctttta cctgctgaac gcacgtgcgc tgaccgaact ggctgatgca
      481 gttgaagctg acgccaaaac ccgccagcgt atccgtttcg ctatcagcca gtgggtcgac
      541 gcgatgagcc cggccaactt cctggccacg aacccggagg cccagcgcct gctgatcgaa
      601 tctggtggtg aatctctgcg tgcgggcgtt cgtaacatga tggaggatct gacgcgcggt
      661 aagatctctc aaaccgacga gagcgcgttt gaagttggcc gtaacgtggc ggttactgaa
      721 ggcgcggttg ttttcgagaa cgaatatttc cagctgctgc aatacaaacc gctgaccgac
      781 aaggttcacg ctcgtccgct gctgatggta ccgccttgca tcaacaaata ctacatcctg
      841 gacctgcaac cagaatcctc tctggttcgt cacgtggttg agcaaggtca caccgtgttc
      901 ctggtttcct ggcgcaaccc ggatgcttct atggcaggtt ctacgtggga tgattacatc
      961 gagcacgcgg ctattcgtgc gatcgaagtt gcacgtgata ttagcggcca ggataagatc
     1021 aacgtactgg gcttttgcgt tggcggtact attgttagca ctgcactggc agttctggcg
     1081 gcacgtggcg agcatccggc tgcatccgtt accctgctga ccaccctgct ggattttgcg
     1141 gacactggca tcctggacgt ctttgttgat gaaggtcacg tgcagctgcg cgaagcgact
     1201 ctgggtggtg gcgcgggtgc tccgtgcgca ctgctgcgcg gcctggaact ggctaacact
     1261 ttctcctttc tgcgtccgaa cgacctggtg tggaactacg ttgtggacaa ctacctgaaa
     1321 ggtaacacgc cggtgccttt cgatctgctg ttctggaacg gcgatgctac caatctgccg
     1381 ggtccatggt attgctggta cctgcgtcac acttatctgc aaaacgaact tactagagcc
     1441 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1501 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1561 gtttata
//