LOCUS       BBa_K156030             1953 bp    DNA     linear       22-Nov-2024
DEFINITION  Genetic attenuator measurement device (template)
ACCESSION   BBa_K156030
VERSION     BBa_K156030.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..901
                     /label=mrfp1
     misc_feature    908..932
                     /label=Help:Barcodes
     CDS             227..907
                     /label=BBa_E1010
     misc_feature    209..932
                     /label=BBa_I13502
     misc_feature    1..932
                     /label=BBa_I715039
     stem_loop       952..995
                     /label=stem_loop
     misc_feature    941..1020
                     /label=BBa_B0010
     terminator      941..1020
                     /label=BBa_B0010
     stem_loop       1036..1055
                     /label=T7 TE
     misc_feature    1062..1062
                     /label=stop
     polyA_site      1056..1069
                     /label=polya
     misc_feature    1029..1069
                     /label=BBa_B0012
     terminator      1029..1069
                     /label=BBa_B0012
     terminator      941..1069
                     /label=BBa_B0015
     RBS             1078..1090
                     /label=RBS-3\Weak
     RBS             1084..1087
                     /label=RBS
     misc_feature    1078..1090
                     /label=BBa_B0032
     RBS             1078..1090
                     /label=BBa_B0032
     CDS             1097..1816
                     /label=GFP protein
     CDS             1097..1816
                     /label=BBa_E0040
     stem_loop       1836..1879
                     /label=stem_loop
     misc_feature    1825..1904
                     /label=BBa_B0010
     terminator      1825..1904
                     /label=BBa_B0010
     stem_loop       1920..1939
                     /label=T7 TE
     misc_feature    1946..1946
                     /label=stop
     polyA_site      1940..1953
                     /label=polya
     misc_feature    1913..1953
                     /label=BBa_B0012
     terminator      1913..1953
                     /label=BBa_B0012
     misc_feature    1078..1953
                     /label=BBa_E0240
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga
      961 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1021 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagtca
     1081 cacaggaaag tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct
     1141 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
     1201 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
     1261 tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc
     1321 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
     1381 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga
     1441 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
     1501 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
     1561 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag
     1621 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
     1681 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
     1741 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
     1801 actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1861 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1921 gctcaccttc gggtgggcct ttctgcgttt ata
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