BBa_K156033 1 BBa_K156033 RBS + phaB1 + double terminator 2008-10-29T12:00:00Z 2015-05-08T01:10:54Z None This composite part is made of a standard ribosomal binding site and a part that codes for an enzyme that catalyzes the formation of 3-hydroxybutyryl-CoA from acetoacetyl-CoA in polyhydroxyalkanoate synthesis, and a terminator. false true _246_ 0 2922 9 It's complicated true This is a composite part. false Daniel R Tarjan component1997087 1 BBa_K156013 component1997090 1 BBa_B0012 component1997085 1 BBa_B0032 component1997088 1 BBa_B0010 annotation1997087 1 BBa_K156013 range1997087 1 20 760 annotation1997090 1 BBa_B0012 range1997090 1 857 897 annotation1997085 1 BBa_B0032 range1997085 1 1 13 annotation1997088 1 BBa_B0010 range1997088 1 769 848 BBa_K156013 1 Bioplastic phaB1 (acetyacetyl-CoA reductase) 2008-10-28T12:00:00Z 2015-05-08T01:10:54Z This coding region is native to Ralstonia eutropha H16. The original sequence was obtained from GenBank (VERSION NC_008313.1, GI:113866031). This part codes for an enzyme that catalyzes the formation of 3-hydroxybutyryl-CoA from acetoacetyl-CoA in polyhydroxyalkanoate synthesis. false false _246_ 0 1475 9 It's complicated false This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI. false George McArthur IV BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation1710 1 RBS range1710 1 7 10 annotation7027 1 BBa_B0032 range7027 1 1 13 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K156032 1 BBa_K156032 RBS + phaB1 + double terminator 2008-10-29T12:00:00Z 2015-05-08T01:10:54Z None. This part is a composite part made of a standard ribosomal binding site, a polyhydroxybutyrate (PHB) synthase, which catalyzes the polymerization of hydroxybutyryl-CoA, and a terminator (B0015). true false _246_ 0 2922 9 Discontinued false This is a composite part. false Daniel R Tarjan component1997080 1 BBa_B0012 component1997075 1 BBa_B0032 component1997078 1 BBa_B0010 component1997077 1 BBa_K156013 annotation1997077 1 BBa_K156013 range1997077 1 20 760 annotation1997075 1 BBa_B0032 range1997075 1 1 13 annotation1997080 1 BBa_B0012 range1997080 1 857 897 annotation1997078 1 BBa_B0010 range1997078 1 769 848 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K156013_sequence 1 atgactcaacgtatcgcatatgtaactggtggtatgggtggtatcggtactgcaatttgccagcgtctggcgaaagacggtttccgtgttgttgcgggctgcggtccgaactccccgcgtcgtgaaaagtggctggaacaacagaaagccctgggcttcgacttcattgcctccgagggtaatgtagctgactgggattccaccaagactgccttcgataaagttaaatctgaagtgggcgaagtagatgtactgatcaacaacgccggtattactcgtgatgtcgtattccgcaaaatgacccgtgcagactgggatgcagttatcgacaccaacctgacgtctctgttcaacgttaccaaacaggttattgatggtatggctgaccgtggctggggccgcatcgtgaacatctctagcgttaacggccaaaaaggccaatttggtcagacgaattacagcacggctaaagcaggcctgcacggtttcaccatggcactggcgcaggaagtggcgaccaaaggtgttaccgttaataccgtttctccaggttacatcgccaccgatatggttaaggctatccgccaagatgttctggacaagatcgtggctaccattccggttaaacgcctgggcctgccggaagaaattgcgtccatctgtgcgtggctgagctccgaagagtctggtttttccaccggtgcggatttctctctgaacggtggtctgcacatgggttga BBa_B0032_sequence 1 tcacacaggaaag BBa_K156032_sequence 1 tcacacaggaaagtactagatgactcaacgtatcgcatatgtaactggtggtatgggtggtatcggtactgcaatttgccagcgtctggcgaaagacggtttccgtgttgttgcgggctgcggtccgaactccccgcgtcgtgaaaagtggctggaacaacagaaagccctgggcttcgacttcattgcctccgagggtaatgtagctgactgggattccaccaagactgccttcgataaagttaaatctgaagtgggcgaagtagatgtactgatcaacaacgccggtattactcgtgatgtcgtattccgcaaaatgacccgtgcagactgggatgcagttatcgacaccaacctgacgtctctgttcaacgttaccaaacaggttattgatggtatggctgaccgtggctggggccgcatcgtgaacatctctagcgttaacggccaaaaaggccaatttggtcagacgaattacagcacggctaaagcaggcctgcacggtttcaccatggcactggcgcaggaagtggcgaccaaaggtgttaccgttaataccgtttctccaggttacatcgccaccgatatggttaaggctatccgccaagatgttctggacaagatcgtggctaccattccggttaaacgcctgggcctgccggaagaaattgcgtccatctgtgcgtggctgagctccgaagagtctggtttttccaccggtgcggatttctctctgaacggtggtctgcacatgggttgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K156033_sequence 1 tcacacaggaaagtactagatgactcaacgtatcgcatatgtaactggtggtatgggtggtatcggtactgcaatttgccagcgtctggcgaaagacggtttccgtgttgttgcgggctgcggtccgaactccccgcgtcgtgaaaagtggctggaacaacagaaagccctgggcttcgacttcattgcctccgagggtaatgtagctgactgggattccaccaagactgccttcgataaagttaaatctgaagtgggcgaagtagatgtactgatcaacaacgccggtattactcgtgatgtcgtattccgcaaaatgacccgtgcagactgggatgcagttatcgacaccaacctgacgtctctgttcaacgttaccaaacaggttattgatggtatggctgaccgtggctggggccgcatcgtgaacatctctagcgttaacggccaaaaaggccaatttggtcagacgaattacagcacggctaaagcaggcctgcacggtttcaccatggcactggcgcaggaagtggcgaccaaaggtgttaccgttaataccgtttctccaggttacatcgccaccgatatggttaaggctatccgccaagatgttctggacaagatcgtggctaccattccggttaaacgcctgggcctgccggaagaaattgcgtccatctgtgcgtggctgagctccgaagagtctggtttttccaccggtgcggatttctctctgaacggtggtctgcacatgggttgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z