BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_K156014
1
Bioplastic
phaC1 (Poly(3-hydroxybutyrate) polymerase)
2008-10-28T12:00:00Z
2015-05-08T01:10:54Z
This coding region is native to Ralstonia eutropha H16. The original sequence was obtained from GenBank (VERSION NC_008313.1, GI:113866031).
This part codes for a polyhydroxybutyrate (PHB) synthase, which catalyzes the polymerization of hydroxybutyryl-CoA.
false
false
_246_
0
1475
9
It's complicated
false
This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.
false
George McArthur IV
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_K156034
1
BBa_K156034
RBS + phaC1 + double terminator
2008-10-29T12:00:00Z
2015-05-08T01:10:54Z
None.
This composite part is made of a standard ribosomal binding site and a part that codes for a polyhydroxybutyrate (PHB) synthase, which catalyzes the polymerization of hydroxybutyryl-CoA, and a terminator.
false
false
_246_
0
2922
9
It's complicated
true
This is a composite part.
false
Daniel R Tarjan
component1997104
1
BBa_B0012
component1997102
1
BBa_B0010
component1997101
1
BBa_K156014
component1997099
1
BBa_B0032
annotation1997101
1
BBa_K156014
range1997101
1
20
1368
annotation1997099
1
BBa_B0032
range1997099
1
1
13
annotation1997102
1
BBa_B0010
range1997102
1
1377
1456
annotation1997104
1
BBa_B0012
range1997104
1
1465
1505
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K156034_sequence
1
tcacacaggaaagtactagatggcaactggtaaaggtgctgctgcatctactcaagaaggtaaatcccagccgttcaaagtgacccctggtccgttcgatccggcgacctggctggaatggagccgccagtggcagggcactgaaggcaacggccacgcagctgcttctggcattccgggtctggacgctctggcgggcgtaaaaatcgcaccggcacaactgggcgacatccagcaacgttatatgaaagatttcagcgcgctgtggcaggcgatggcggaaggcaaagccgaagcgaccggtccgctgcacgaccgtcgtttcgcaggtgacgcgtggcgtaccaacctgccataccgtttcgctgccgccttttacctgctgaacgcacgtgcgctgaccgaactggctgatgcagttgaagctgacgccaaaacccgccagcgtatccgtttcgctatcagccagtgggtcgacgcgatgagcccggccaacttcctggccacgaacccggaggcccagcgcctgctgatcgaatctggtggtgaatctctgcgtgcgggcgttcgtaacatgatggaggatctgacgcgcggtaagatctctcaaaccgacgagagcgcgtttgaagttggccgtaacgtggcggttactgaaggcgcggttgttttcgagaacgaatatttccagctgctgcaatacaaaccgctgaccgacaaggttcacgctcgtccgctgctgatggtaccgccttgcatcaacaaatactacatcctggacctgcaaccagaatcctctctggttcgtcacgtggttgagcaaggtcacaccgtgttcctggtttcctggcgcaacccggatgcttctatggcaggttctacgtgggatgattacatcgagcacgcggctattcgtgcgatcgaagttgcacgtgatattagcggccaggataagatcaacgtactgggcttttgcgttggcggtactattgttagcactgcactggcagttctggcggcacgtggcgagcatccggctgcatccgttaccctgctgaccaccctgctggattttgcggacactggcatcctggacgtctttgttgatgaaggtcacgtgcagctgcgcgaagcgactctgggtggtggcgcgggtgctccgtgcgcactgctgcgcggcctggaactggctaacactttctcctttctgcgtccgaacgacctggtgtggaactacgttgtggacaactacctgaaaggtaacacgccggtgcctttcgatctgctgttctggaacggcgatgctaccaatctgccgggtccatggtattgctggtacctgcgtcacacttatctgcaaaacgaacttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K156014_sequence
1
atggcaactggtaaaggtgctgctgcatctactcaagaaggtaaatcccagccgttcaaagtgacccctggtccgttcgatccggcgacctggctggaatggagccgccagtggcagggcactgaaggcaacggccacgcagctgcttctggcattccgggtctggacgctctggcgggcgtaaaaatcgcaccggcacaactgggcgacatccagcaacgttatatgaaagatttcagcgcgctgtggcaggcgatggcggaaggcaaagccgaagcgaccggtccgctgcacgaccgtcgtttcgcaggtgacgcgtggcgtaccaacctgccataccgtttcgctgccgccttttacctgctgaacgcacgtgcgctgaccgaactggctgatgcagttgaagctgacgccaaaacccgccagcgtatccgtttcgctatcagccagtgggtcgacgcgatgagcccggccaacttcctggccacgaacccggaggcccagcgcctgctgatcgaatctggtggtgaatctctgcgtgcgggcgttcgtaacatgatggaggatctgacgcgcggtaagatctctcaaaccgacgagagcgcgtttgaagttggccgtaacgtggcggttactgaaggcgcggttgttttcgagaacgaatatttccagctgctgcaatacaaaccgctgaccgacaaggttcacgctcgtccgctgctgatggtaccgccttgcatcaacaaatactacatcctggacctgcaaccagaatcctctctggttcgtcacgtggttgagcaaggtcacaccgtgttcctggtttcctggcgcaacccggatgcttctatggcaggttctacgtgggatgattacatcgagcacgcggctattcgtgcgatcgaagttgcacgtgatattagcggccaggataagatcaacgtactgggcttttgcgttggcggtactattgttagcactgcactggcagttctggcggcacgtggcgagcatccggctgcatccgttaccctgctgaccaccctgctggattttgcggacactggcatcctggacgtctttgttgatgaaggtcacgtgcagctgcgcgaagcgactctgggtggtggcgcgggtgctccgtgcgcactgctgcgcggcctggaactggctaacactttctcctttctgcgtccgaacgacctggtgtggaactacgttgtggacaactacctgaaaggtaacacgccggtgcctttcgatctgctgttctggaacggcgatgctaccaatctgccgggtccatggtattgctggtacctgcgtcacacttatctgcaaaacgaact
BBa_B0032_sequence
1
tcacacaggaaag
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z