BBa_K156035 1 BBa_K156035 Promoter + RBS + OFP 2008-10-29T12:00:00Z 2015-05-08T01:10:54Z None. This composite part is made of a promoter, a standard ribosomal binding site and a part that codes for orange fluorescent protein, which can be used as a reporter. false false _246_ 0 2922 9 It's complicated false This is a composite part. false Daniel R Tarjan component1997659 1 BBa_B0032 component1997661 1 BBa_K156009 component1997653 1 BBa_R0040 annotation1997653 1 BBa_R0040 range1997653 1 1 54 annotation1997661 1 BBa_K156009 range1997661 1 82 945 annotation1997659 1 BBa_B0032 range1997659 1 63 75 BBa_K156009 1 BBa_K156009 OFP (orange fluorescent protein) 2008-10-27T12:00:00Z 2015-05-08T01:10:54Z OFP was cloned from the tentacles of Cerianthus sp., a tube anemone. The original sequence was obtained from GenBank (VERSION AY296063.1, GI:31616578). Fluorescent proteins are useful tools for visualizing and quantifying protein expression in cells. OFP is yet another fluorescent protein, like GFP, that can be utilized as a reporter. false false _246_ 0 1475 9 It's complicated false This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI. false George McArthur IV BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation1710 1 RBS range1710 1 7 10 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986786 1 TetR 2 range1986786 1 26 44 annotation1986785 1 -35 range1986785 1 20 25 annotation1986787 1 -10 range1986787 1 43 48 BBa_K156035_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtactagatgaacctgtccaaaaacgtatctgtgtccgtgtacatgaaaggcaatgtaaacaaccacgagttcgaatacgacggcgaaggcggtggtgatccatataccggcaaatacagcatgaagatgaccctgcgtggtcagaactgtctgccgtttagctatgacatcatcaccacggcattccagtacggcttccgtgtttttactaaatacccggaaggtatcgttgattatttcaaagactccctgccagatgcctttcaatggaaccgccgtattgttttcgaggacggcggcgtcctgaacatgagcagcgatattacttataaagacaacgtcctgcacggcgatgtgtgggcggttggtgtgaacttccctccgaacggtcctgtaatgaaaaacgaaattgtgatggaggaaccgacggaagaaacctttacgccgaaaaacggcgtactggtcggtttttgcccgaaagcctacctgctgaaagatggttcttattactacggtaatatgaccaccttttaccgtagcaagaaaagcggccaggccccaccaggctatcatttcgttaaacaccgtctggtaaaaaccaatgtaggtcacggcttcaaaacggttgaacagaccgaatacgcaaccgcgcacgttagcgatctgccaaaataacacaaatgactgcaaccgctgctgaacaagtgactggccactctggtgagctaaaaaccgtttcaggttctgctgcatctgtgctctttcactttcgtcgccaaacgttccaagaacgacttcctgttcgcggtgacttctgcgctgtgactggaataaaccatcctgctgtacaccaaaaagaaaaagaaaaag BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_B0032_sequence 1 tcacacaggaaag BBa_K156009_sequence 1 atgaacctgtccaaaaacgtatctgtgtccgtgtacatgaaaggcaatgtaaacaaccacgagttcgaatacgacggcgaaggcggtggtgatccatataccggcaaatacagcatgaagatgaccctgcgtggtcagaactgtctgccgtttagctatgacatcatcaccacggcattccagtacggcttccgtgtttttactaaatacccggaaggtatcgttgattatttcaaagactccctgccagatgcctttcaatggaaccgccgtattgttttcgaggacggcggcgtcctgaacatgagcagcgatattacttataaagacaacgtcctgcacggcgatgtgtgggcggttggtgtgaacttccctccgaacggtcctgtaatgaaaaacgaaattgtgatggaggaaccgacggaagaaacctttacgccgaaaaacggcgtactggtcggtttttgcccgaaagcctacctgctgaaagatggttcttattactacggtaatatgaccaccttttaccgtagcaagaaaagcggccaggccccaccaggctatcatttcgttaaacaccgtctggtaaaaaccaatgtaggtcacggcttcaaaacggttgaacagaccgaatacgcaaccgcgcacgttagcgatctgccaaaataacacaaatgactgcaaccgctgctgaacaagtgactggccactctggtgagctaaaaaccgtttcaggttctgctgcatctgtgctctttcactttcgtcgccaaacgttccaagaacgacttcctgttcgcggtgacttctgcgctgtgactggaataaaccatcctgctgtacaccaaaaagaaaaagaaaaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z