LOCUS       BBa_K1565001            3256 bp    DNA     linear       09-May-2024
DEFINITION  Colorimetric indicator of cell lysis
ACCESSION   BBa_K1565001
VERSION     BBa_K1565001.1  
FEATURES             Location/Qualifiers
     promoter        1..122
                     /label=pRha
     promoter        1..122
                     /label=BBa_K914003
     misc_feature    129..131
                     /label=start codon
     CDS             132..1361
                     /label=protein
     CDS             129..1361
                     /label=BBa_K805011
     promoter        1370..1461
                     /label=PO
     promoter        1370..1461
                     /label=BBa_I746361
     misc_feature    1470..1473
                     /label=BglII
     misc_feature    1474..1474
                     /label=ATG
     misc_feature    2398..2399
                     /label=GG
     CDS             1474..2397
                     /label=INPNC
     CDS             1470..2399
                     /label=BBa_K523008
     CDS             2406..3116
                     /label=fwYellow
     CDS             2406..3119
                     /label=BBa_K1033910
     stem_loop       3139..3182
                     /label=stem_loop
     misc_feature    3128..3207
                     /label=BBa_B0010
     terminator      3128..3207
                     /label=BBa_B0010
     stem_loop       3223..3242
                     /label=T7 TE
     misc_feature    3249..3249
                     /label=stop
     polyA_site      3243..3256
                     /label=polya
     misc_feature    3216..3256
                     /label=BBa_B0012
     terminator      3216..3256
                     /label=BBa_B0012
     terminator      3128..3256
                     /label=BBa_B0015
ORIGIN
        1 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
       61 attttcctgt cagtaacgag aaggtcgcgt attcaggcgc tttttagact ggtcgtaatg
      121 aatactagat gagatcctca cataagcgta atttggctgc gctgctttcc atcagcggag
      181 ccgcggtggc acagcagacg gcatggggtc aatgcggagg acaaggatgg tccggtgcga
      241 catcatgtgt gtcaggctac acttgctcct acacaaatga ctggtactcc cagtgcgttc
      301 ctggatcatc cggcggcgga ggtagcaacc ctacaacgac caccacgatg atccggacga
      361 ccacgacggc ttcctcctcg ggccccaccg gcggcgctgg tggtggtaag gttcgatttg
      421 ccggtgtcaa catcgccggc tttgatttcg gagtggtgat cagcggcact caagacatga
      481 cccaaattgt ggacgagtcc gtcgatggtg tcaatcagat gcgacacttt gtcaacgacg
      541 acggcttcaa catcttccgc ctcccgtcag gctggcagtt cctggtgaac aacaacctcg
      601 gtggctcact ggactcgaac aatttcgcca agtacgacaa gctggttcaa ggatgtctgt
      661 ccctgggagc atactgtatc gtcgatgtcc acaattacgc tcgctggaac ggcggcgtca
      721 tcggacaagg cggtcccacc gacgaccagt tcaccagtct ctggactcaa ctggccactc
      781 actacaagag tgaaagcaag atcatctttg gtgtgatgaa cgagccacac gacctggaca
      841 tcaaccgatg ggccaccacc gtgcaaaagg ccgtgacggc gatccgcaag gccggcgcca
      901 cttcccaaat gatccttctt ccaggtaccg acttcaccag tgcggccaac tttgtcgaga
      961 atggctccgg tgccgcgctc tcggccgtca ccaacctgga cggatccacc accaacctga
     1021 tcttcgacgt gcacaagtat ctcgactccg ataacagcgg cacccacgcc gagtgcgtga
     1081 ccaacaacgc cgacgctttc aacagcctgg cccaatggct ccgcaccaac aagcgtcaag
     1141 ccatgctcac cgagaccggt ggtggtaacg tccagagctg cggaacatac atgtgccagc
     1201 aactggatgt gctcaaccaa aactctgacg tttaccttgg atggacctcg tggagcgctg
     1261 gaggtttcca ggtttcctgg aactatgtgc tgggtgaggt cccaaccaac aacgttgata
     1321 cctacctggt caagcaatgc tttgttccca agtggaagaa ctactagagc gcgccccgcg
     1381 attcgctaag gtgctgttgt gtcagtgata agccatccgg gactgatggc ggaggatgcg
     1441 catcgtcggg aaactgatgc ctactagaga tctatgaccc tggataaagc gctggtgctg
     1501 cgcacttgtg ccaataatat ggccgaccat tgtggcctga tctggcctgc ttctggtaca
     1561 gttgaaagcc gctattggca gtctacacgt cgtcacgaaa acgggctggt tgggctgctg
     1621 tggggtgccg gaacatccgc ctttctgagc gttcatgccg atgctcgttg gattgtttgt
     1681 gaggttgctg ttgccgacat cattagcctg gaagaaccgg gtatggtgaa atttcctcgt
     1741 gctgaagtgg tccatgttgg tgatcgtatt agcgcctccc atttcatttc agctcgtcag
     1801 gctgatccag caagcacatc aacaagcacc tctaccagta cactgacacc aatgccaacc
     1861 gctatcccta ctcctatgcc ggctgtagcc tctgttactc tgcctgtggc cgaacaagct
     1921 cgccacgaag tctttgacgt tgcctctgtg tctgctgctg ctgctcctgt taacactctg
     1981 cctgttacca cgcctcaaaa tctgcaaacg gcgacctatg gctcaacact gagtggtgat
     2041 aaccattctc gtctgattgc cgggtatggc tctaacgaaa ccgccggaaa ccattctgat
     2101 ctgatcggtg gtcacgattg tacgctgatg gcaggtgatc aatctcgtct gaccgctggc
     2161 aaaaattccg ttctgaccgc tggggcacgt tctaaactga tcgggagcga aggctctact
     2221 ctgtccgccg gagaagattc taccctgatc tttcgtctgt gggatggtaa acgttatcgt
     2281 cagctggtcg ctcgtacagg tgaaaatggt gtggaagccg acattccgta ttatgtgaat
     2341 gaggacgatg atattgtgga caaaccggac gaagatgatg attggatcga agtgaaaggt
     2401 actagatgac ggcactgact gaaggcgcaa aactgttcga gaaagaaatc ccatatatca
     2461 ctgagctgga aggtgacgtt gaaggtatga agtttatcat caagggtgaa ggtaccggtg
     2521 acgcgagcgt cggtaaagtg gatgctcagt tcatttgtac cacgggcgac gttccggttc
     2581 cgtggagcac gctggtcacc acgctgacgt atggtgctca gtgctttgcc aagtatccgc
     2641 gccacattgc ggatttcttc aaaagctgca tgccggaagg ttacgtccaa gagcgcacca
     2701 tcacctttga gggtgatggc gtgttcaaga cccgtgcgga agtcaccttt gaaaatggca
     2761 gcgtgtacaa ccgtgtaaaa ctgaacggcc agggtttcaa gaaggacggc cacgtgctgg
     2821 gcaaaaatct ggagtttaac tttacccctc attgtttgta catttggggt gaccaagcga
     2881 atcatggcct gaagagcgcg ttcaaaatca tgcatgagat caccggctcc aaagaggatt
     2941 tcattgttgc cgatcacacc caaatgaata ccccgattgg tggtggtccg gtgcacgtgc
     3001 cggagtacca ccacattacg tatcatgtta ccctgtctaa agacgtcacc gatcaccgtg
     3061 accatttgaa cattgttgag gtgatcaagg cagttgacct ggagacgtac cgttaataat
     3121 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     3181 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     3241 cctttctgcg tttata
//