BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_K805011 1 Cel5A Cel5A, endoglucanases 2012-09-19T11:00:00Z 2015-06-19T01:48:30Z yes Released HQ 2013 test false false _1062_ 4206 11895 9 In stock true test false Liu Ping annotation2200608 1 protein range2200608 1 4 1233 annotation2200607 1 start codon range2200607 1 1 3 BBa_K523008 1 BBa_K523008 INP for BioSandwich 2011-08-03T11:00:00Z 2015-05-08T01:12:33Z From <partinfo>BBa_K265008</partinfo>. Released HQ 2013 This is a modified version of <partinfo>BBa_K265008</partinfo> for use with the BioSandwich protocol of Edinburgh 2011. false false _688_ 0 8799 9 In stock false N/A. false Mun Ching Lee, Sylvia Ispasanie annotation2124328 1 INPNC range2124328 1 5 928 annotation2124327 1 GG range2124327 1 929 930 annotation2124326 1 ATG range2124326 1 5 5 annotation2124325 1 BglII range2124325 1 1 4 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_I746361 1 BBa_I746361 PO promoter from P2 phage 2007-09-10T11:00:00Z 2015-08-31T04:08:04Z The source of the DNA is the P2 phage genome. Released HQ 2013 This is the PO promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PO promoter. false false _116_ 0 2122 9 In stock false no special considerations true Stefan Milde annotation1943784 1 PO range1943784 1 1 92 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K914003 1 BBa_K914003 L-rhamnose-inducible promoter (pRha) 2012-09-19T11:00:00Z 2015-05-08T01:13:45Z Amplification of the plasmid pJOE3075 (Dr. Altenbuchner). Released HQ 2013 L-rhamnose-inducible promoter is capable of high-level recombinant protein expression in the presence of L-rhamnose, it is also tightly regulated in the absence of L-rhamnose by the addition of D-glucose. false false _1179_ 0 13487 9 In stock true One base pair modified (90: A -> T) to avoid EcoRI site. Mutation made in a less conserved base pair. false Denis Samuylov annotation2188559 1 pRha range2188559 1 1 122 BBa_K1033910 1 fwYellow fwYellow, yellow chromoprotein 2013-09-16T11:00:00Z 2016-01-25T02:41:53Z Synthetic chromoprotein from DNA 2.0. This chromoprotein naturally exhibits strong color when expressed. false false _1340_ 4206 13997 9 In stock false This construct is synthetic. It was synthesized from a template where the consensus sequence for the "color dependancy" in order to obtain a chromoprotein with a unique yellow color. false Sabri Jamal annotation2371218 1 fwYellow range2371218 1 1 711 BBa_K1565001 1 ColiAlert Colorimetric indicator of cell lysis 2014-10-09T11:00:00Z 2015-05-08T01:10:54Z It is formed by biobricks already available in the registry. This biobrick makes E. coli to have pink color under natural conditions. It is formed by biobricks already available in the registry. It is responsible for the production of yellow chromoprotein (BBa_K1033910) which in the presence of basic media change their color to pink. This part can be used as a reporter cell lysis. false false _1951_ 0 22300 9 It's complicated false The endoglucanase gene was added to biobrick wrongly. The biobrick works and should be made &#8203;&#8203;without this gene. false Deborah M Landim Zanforlin component2415887 1 BBa_K1033910 component2415885 1 BBa_K523008 component2415878 1 BBa_K805011 component2415894 1 BBa_B0015 component2415875 1 BBa_K914003 component2415880 1 BBa_I746361 annotation2415880 1 BBa_I746361 range2415880 1 1370 1461 annotation2415885 1 BBa_K523008 range2415885 1 1470 2399 annotation2415887 1 BBa_K1033910 range2415887 1 2406 3119 annotation2415894 1 BBa_B0015 range2415894 1 3128 3256 annotation2415878 1 BBa_K805011 range2415878 1 129 1361 annotation2415875 1 BBa_K914003 range2415875 1 1 122 BBa_K523008_sequence 1 atctatgaccctggataaagcgctggtgctgcgcacttgtgccaataatatggccgaccattgtggcctgatctggcctgcttctggtacagttgaaagccgctattggcagtctacacgtcgtcacgaaaacgggctggttgggctgctgtggggtgccggaacatccgcctttctgagcgttcatgccgatgctcgttggattgtttgtgaggttgctgttgccgacatcattagcctggaagaaccgggtatggtgaaatttcctcgtgctgaagtggtccatgttggtgatcgtattagcgcctcccatttcatttcagctcgtcaggctgatccagcaagcacatcaacaagcacctctaccagtacactgacaccaatgccaaccgctatccctactcctatgccggctgtagcctctgttactctgcctgtggccgaacaagctcgccacgaagtctttgacgttgcctctgtgtctgctgctgctgctcctgttaacactctgcctgttaccacgcctcaaaatctgcaaacggcgacctatggctcaacactgagtggtgataaccattctcgtctgattgccgggtatggctctaacgaaaccgccggaaaccattctgatctgatcggtggtcacgattgtacgctgatggcaggtgatcaatctcgtctgaccgctggcaaaaattccgttctgaccgctggggcacgttctaaactgatcgggagcgaaggctctactctgtccgccggagaagattctaccctgatctttcgtctgtgggatggtaaacgttatcgtcagctggtcgctcgtacaggtgaaaatggtgtggaagccgacattccgtattatgtgaatgaggacgatgatattgtggacaaaccggacgaagatgatgattggatcgaagtgaaagg BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_I746361_sequence 1 cgcgccccgcgattcgctaaggtgctgttgtgtcagtgataagccatccgggactgatggcggaggatgcgcatcgtcgggaaactgatgcc BBa_K805011_sequence 1 atgagatcctcacataagcgtaatttggctgcgctgctttccatcagcggagccgcggtggcacagcagacggcatggggtcaatgcggaggacaaggatggtccggtgcgacatcatgtgtgtcaggctacacttgctcctacacaaatgactggtactcccagtgcgttcctggatcatccggcggcggaggtagcaaccctacaacgaccaccacgatgatccggacgaccacgacggcttcctcctcgggccccaccggcggcgctggtggtggtaaggttcgatttgccggtgtcaacatcgccggctttgatttcggagtggtgatcagcggcactcaagacatgacccaaattgtggacgagtccgtcgatggtgtcaatcagatgcgacactttgtcaacgacgacggcttcaacatcttccgcctcccgtcaggctggcagttcctggtgaacaacaacctcggtggctcactggactcgaacaatttcgccaagtacgacaagctggttcaaggatgtctgtccctgggagcatactgtatcgtcgatgtccacaattacgctcgctggaacggcggcgtcatcggacaaggcggtcccaccgacgaccagttcaccagtctctggactcaactggccactcactacaagagtgaaagcaagatcatctttggtgtgatgaacgagccacacgacctggacatcaaccgatgggccaccaccgtgcaaaaggccgtgacggcgatccgcaaggccggcgccacttcccaaatgatccttcttccaggtaccgacttcaccagtgcggccaactttgtcgagaatggctccggtgccgcgctctcggccgtcaccaacctggacggatccaccaccaacctgatcttcgacgtgcacaagtatctcgactccgataacagcggcacccacgccgagtgcgtgaccaacaacgccgacgctttcaacagcctggcccaatggctccgcaccaacaagcgtcaagccatgctcaccgagaccggtggtggtaacgtccagagctgcggaacatacatgtgccagcaactggatgtgctcaaccaaaactctgacgtttaccttggatggacctcgtggagcgctggaggtttccaggtttcctggaactatgtgctgggtgaggtcccaaccaacaacgttgatacctacctggtcaagcaatgctttgttcccaagtggaagaac BBa_K1033910_sequence 1 atgacggcactgactgaaggcgcaaaactgttcgagaaagaaatcccatatatcactgagctggaaggtgacgttgaaggtatgaagtttatcatcaagggtgaaggtaccggtgacgcgagcgtcggtaaagtggatgctcagttcatttgtaccacgggcgacgttccggttccgtggagcacgctggtcaccacgctgacgtatggtgctcagtgctttgccaagtatccgcgccacattgcggatttcttcaaaagctgcatgccggaaggttacgtccaagagcgcaccatcacctttgagggtgatggcgtgttcaagacccgtgcggaagtcacctttgaaaatggcagcgtgtacaaccgtgtaaaactgaacggccagggtttcaagaaggacggccacgtgctgggcaaaaatctggagtttaactttacccctcattgtttgtacatttggggtgaccaagcgaatcatggcctgaagagcgcgttcaaaatcatgcatgagatcaccggctccaaagaggatttcattgttgccgatcacacccaaatgaataccccgattggtggtggtccggtgcacgtgccggagtaccaccacattacgtatcatgttaccctgtctaaagacgtcaccgatcaccgtgaccatttgaacattgttgaggtgatcaaggcagttgacctggagacgtaccgttaataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K914003_sequence 1 ccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaa BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1565001_sequence 1 ccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaatactagatgagatcctcacataagcgtaatttggctgcgctgctttccatcagcggagccgcggtggcacagcagacggcatggggtcaatgcggaggacaaggatggtccggtgcgacatcatgtgtgtcaggctacacttgctcctacacaaatgactggtactcccagtgcgttcctggatcatccggcggcggaggtagcaaccctacaacgaccaccacgatgatccggacgaccacgacggcttcctcctcgggccccaccggcggcgctggtggtggtaaggttcgatttgccggtgtcaacatcgccggctttgatttcggagtggtgatcagcggcactcaagacatgacccaaattgtggacgagtccgtcgatggtgtcaatcagatgcgacactttgtcaacgacgacggcttcaacatcttccgcctcccgtcaggctggcagttcctggtgaacaacaacctcggtggctcactggactcgaacaatttcgccaagtacgacaagctggttcaaggatgtctgtccctgggagcatactgtatcgtcgatgtccacaattacgctcgctggaacggcggcgtcatcggacaaggcggtcccaccgacgaccagttcaccagtctctggactcaactggccactcactacaagagtgaaagcaagatcatctttggtgtgatgaacgagccacacgacctggacatcaaccgatgggccaccaccgtgcaaaaggccgtgacggcgatccgcaaggccggcgccacttcccaaatgatccttcttccaggtaccgacttcaccagtgcggccaactttgtcgagaatggctccggtgccgcgctctcggccgtcaccaacctggacggatccaccaccaacctgatcttcgacgtgcacaagtatctcgactccgataacagcggcacccacgccgagtgcgtgaccaacaacgccgacgctttcaacagcctggcccaatggctccgcaccaacaagcgtcaagccatgctcaccgagaccggtggtggtaacgtccagagctgcggaacatacatgtgccagcaactggatgtgctcaaccaaaactctgacgtttaccttggatggacctcgtggagcgctggaggtttccaggtttcctggaactatgtgctgggtgaggtcccaaccaacaacgttgatacctacctggtcaagcaatgctttgttcccaagtggaagaactactagagcgcgccccgcgattcgctaaggtgctgttgtgtcagtgataagccatccgggactgatggcggaggatgcgcatcgtcgggaaactgatgcctactagagatctatgaccctggataaagcgctggtgctgcgcacttgtgccaataatatggccgaccattgtggcctgatctggcctgcttctggtacagttgaaagccgctattggcagtctacacgtcgtcacgaaaacgggctggttgggctgctgtggggtgccggaacatccgcctttctgagcgttcatgccgatgctcgttggattgtttgtgaggttgctgttgccgacatcattagcctggaagaaccgggtatggtgaaatttcctcgtgctgaagtggtccatgttggtgatcgtattagcgcctcccatttcatttcagctcgtcaggctgatccagcaagcacatcaacaagcacctctaccagtacactgacaccaatgccaaccgctatccctactcctatgccggctgtagcctctgttactctgcctgtggccgaacaagctcgccacgaagtctttgacgttgcctctgtgtctgctgctgctgctcctgttaacactctgcctgttaccacgcctcaaaatctgcaaacggcgacctatggctcaacactgagtggtgataaccattctcgtctgattgccgggtatggctctaacgaaaccgccggaaaccattctgatctgatcggtggtcacgattgtacgctgatggcaggtgatcaatctcgtctgaccgctggcaaaaattccgttctgaccgctggggcacgttctaaactgatcgggagcgaaggctctactctgtccgccggagaagattctaccctgatctttcgtctgtgggatggtaaacgttatcgtcagctggtcgctcgtacaggtgaaaatggtgtggaagccgacattccgtattatgtgaatgaggacgatgatattgtggacaaaccggacgaagatgatgattggatcgaagtgaaaggtactagatgacggcactgactgaaggcgcaaaactgttcgagaaagaaatcccatatatcactgagctggaaggtgacgttgaaggtatgaagtttatcatcaagggtgaaggtaccggtgacgcgagcgtcggtaaagtggatgctcagttcatttgtaccacgggcgacgttccggttccgtggagcacgctggtcaccacgctgacgtatggtgctcagtgctttgccaagtatccgcgccacattgcggatttcttcaaaagctgcatgccggaaggttacgtccaagagcgcaccatcacctttgagggtgatggcgtgttcaagacccgtgcggaagtcacctttgaaaatggcagcgtgtacaaccgtgtaaaactgaacggccagggtttcaagaaggacggccacgtgctgggcaaaaatctggagtttaactttacccctcattgtttgtacatttggggtgaccaagcgaatcatggcctgaagagcgcgttcaaaatcatgcatgagatcaccggctccaaagaggatttcattgttgccgatcacacccaaatgaataccccgattggtggtggtccggtgcacgtgccggagtaccaccacattacgtatcatgttaccctgtctaaagacgtcaccgatcaccgtgaccatttgaacattgttgaggtgatcaaggcagttgacctggagacgtaccgttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z