BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_K914003
1
BBa_K914003
L-rhamnose-inducible promoter (pRha)
2012-09-19T11:00:00Z
2015-05-08T01:13:45Z
Amplification of the plasmid pJOE3075 (Dr. Altenbuchner).
Released HQ 2013
L-rhamnose-inducible promoter is capable of high-level recombinant protein expression in the presence of L-rhamnose, it is also tightly regulated in the absence of L-rhamnose by the addition of D-glucose.
false
false
_1179_
0
13487
9
In stock
true
One base pair modified (90: A -> T) to avoid EcoRI site. Mutation made in a less conserved base pair.
false
Denis Samuylov
annotation2188559
1
pRha
range2188559
1
1
122
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_K1565001
1
ColiAlert
Colorimetric indicator of cell lysis
2014-10-09T11:00:00Z
2015-05-08T01:10:54Z
It is formed by biobricks already available in the registry.
This biobrick makes E. coli to have pink color under natural conditions. It is formed by biobricks already available in the registry. It is responsible for the production of yellow chromoprotein (BBa_K1033910) which in the presence of basic media change their color to pink. This part can be used as a reporter cell lysis.
false
false
_1951_
0
22300
9
It's complicated
false
The endoglucanase gene was added to biobrick wrongly. The biobrick works and should be made ​​without this gene.
false
Deborah M Landim Zanforlin
component2415894
1
BBa_B0015
component2415880
1
BBa_I746361
component2415878
1
BBa_K805011
component2415885
1
BBa_K523008
component2415887
1
BBa_K1033910
component2415875
1
BBa_K914003
annotation2415887
1
BBa_K1033910
range2415887
1
2406
3119
annotation2415875
1
BBa_K914003
range2415875
1
1
122
annotation2415880
1
BBa_I746361
range2415880
1
1370
1461
annotation2415878
1
BBa_K805011
range2415878
1
129
1361
annotation2415894
1
BBa_B0015
range2415894
1
3128
3256
annotation2415885
1
BBa_K523008
range2415885
1
1470
2399
BBa_K805011
1
Cel5A
Cel5A, endoglucanases
2012-09-19T11:00:00Z
2015-06-19T01:48:30Z
yes
Released HQ 2013
test
false
false
_1062_
4206
11895
9
In stock
true
test
false
Liu Ping
annotation2200607
1
start codon
range2200607
1
1
3
annotation2200608
1
protein
range2200608
1
4
1233
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_K1033910
1
fwYellow
fwYellow, yellow chromoprotein
2013-09-16T11:00:00Z
2016-01-25T02:41:53Z
Synthetic chromoprotein from DNA 2.0.
This chromoprotein naturally exhibits strong color when expressed.
false
false
_1340_
4206
13997
9
In stock
false
This construct is synthetic. It was synthesized from a template where the consensus sequence for the "color dependancy" in order to obtain a chromoprotein with a unique yellow color.
false
Sabri Jamal
annotation2371218
1
fwYellow
range2371218
1
1
711
BBa_K523008
1
BBa_K523008
INP for BioSandwich
2011-08-03T11:00:00Z
2015-05-08T01:12:33Z
From <partinfo>BBa_K265008</partinfo>.
Released HQ 2013
This is a modified version of <partinfo>BBa_K265008</partinfo> for use with the BioSandwich protocol of Edinburgh 2011.
false
false
_688_
0
8799
9
In stock
false
N/A.
false
Mun Ching Lee, Sylvia Ispasanie
annotation2124327
1
GG
range2124327
1
929
930
annotation2124328
1
INPNC
range2124328
1
5
928
annotation2124326
1
ATG
range2124326
1
5
5
annotation2124325
1
BglII
range2124325
1
1
4
BBa_I746361
1
BBa_I746361
PO promoter from P2 phage
2007-09-10T11:00:00Z
2015-08-31T04:08:04Z
The source of the DNA is the P2 phage genome.
Released HQ 2013
This is the PO promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PO promoter.
false
false
_116_
0
2122
9
In stock
false
no special considerations
true
Stefan Milde
annotation1943784
1
PO
range1943784
1
1
92
BBa_K523008_sequence
1
atctatgaccctggataaagcgctggtgctgcgcacttgtgccaataatatggccgaccattgtggcctgatctggcctgcttctggtacagttgaaagccgctattggcagtctacacgtcgtcacgaaaacgggctggttgggctgctgtggggtgccggaacatccgcctttctgagcgttcatgccgatgctcgttggattgtttgtgaggttgctgttgccgacatcattagcctggaagaaccgggtatggtgaaatttcctcgtgctgaagtggtccatgttggtgatcgtattagcgcctcccatttcatttcagctcgtcaggctgatccagcaagcacatcaacaagcacctctaccagtacactgacaccaatgccaaccgctatccctactcctatgccggctgtagcctctgttactctgcctgtggccgaacaagctcgccacgaagtctttgacgttgcctctgtgtctgctgctgctgctcctgttaacactctgcctgttaccacgcctcaaaatctgcaaacggcgacctatggctcaacactgagtggtgataaccattctcgtctgattgccgggtatggctctaacgaaaccgccggaaaccattctgatctgatcggtggtcacgattgtacgctgatggcaggtgatcaatctcgtctgaccgctggcaaaaattccgttctgaccgctggggcacgttctaaactgatcgggagcgaaggctctactctgtccgccggagaagattctaccctgatctttcgtctgtgggatggtaaacgttatcgtcagctggtcgctcgtacaggtgaaaatggtgtggaagccgacattccgtattatgtgaatgaggacgatgatattgtggacaaaccggacgaagatgatgattggatcgaagtgaaagg
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_I746361_sequence
1
cgcgccccgcgattcgctaaggtgctgttgtgtcagtgataagccatccgggactgatggcggaggatgcgcatcgtcgggaaactgatgcc
BBa_K805011_sequence
1
atgagatcctcacataagcgtaatttggctgcgctgctttccatcagcggagccgcggtggcacagcagacggcatggggtcaatgcggaggacaaggatggtccggtgcgacatcatgtgtgtcaggctacacttgctcctacacaaatgactggtactcccagtgcgttcctggatcatccggcggcggaggtagcaaccctacaacgaccaccacgatgatccggacgaccacgacggcttcctcctcgggccccaccggcggcgctggtggtggtaaggttcgatttgccggtgtcaacatcgccggctttgatttcggagtggtgatcagcggcactcaagacatgacccaaattgtggacgagtccgtcgatggtgtcaatcagatgcgacactttgtcaacgacgacggcttcaacatcttccgcctcccgtcaggctggcagttcctggtgaacaacaacctcggtggctcactggactcgaacaatttcgccaagtacgacaagctggttcaaggatgtctgtccctgggagcatactgtatcgtcgatgtccacaattacgctcgctggaacggcggcgtcatcggacaaggcggtcccaccgacgaccagttcaccagtctctggactcaactggccactcactacaagagtgaaagcaagatcatctttggtgtgatgaacgagccacacgacctggacatcaaccgatgggccaccaccgtgcaaaaggccgtgacggcgatccgcaaggccggcgccacttcccaaatgatccttcttccaggtaccgacttcaccagtgcggccaactttgtcgagaatggctccggtgccgcgctctcggccgtcaccaacctggacggatccaccaccaacctgatcttcgacgtgcacaagtatctcgactccgataacagcggcacccacgccgagtgcgtgaccaacaacgccgacgctttcaacagcctggcccaatggctccgcaccaacaagcgtcaagccatgctcaccgagaccggtggtggtaacgtccagagctgcggaacatacatgtgccagcaactggatgtgctcaaccaaaactctgacgtttaccttggatggacctcgtggagcgctggaggtttccaggtttcctggaactatgtgctgggtgaggtcccaaccaacaacgttgatacctacctggtcaagcaatgctttgttcccaagtggaagaac
BBa_K1033910_sequence
1
atgacggcactgactgaaggcgcaaaactgttcgagaaagaaatcccatatatcactgagctggaaggtgacgttgaaggtatgaagtttatcatcaagggtgaaggtaccggtgacgcgagcgtcggtaaagtggatgctcagttcatttgtaccacgggcgacgttccggttccgtggagcacgctggtcaccacgctgacgtatggtgctcagtgctttgccaagtatccgcgccacattgcggatttcttcaaaagctgcatgccggaaggttacgtccaagagcgcaccatcacctttgagggtgatggcgtgttcaagacccgtgcggaagtcacctttgaaaatggcagcgtgtacaaccgtgtaaaactgaacggccagggtttcaagaaggacggccacgtgctgggcaaaaatctggagtttaactttacccctcattgtttgtacatttggggtgaccaagcgaatcatggcctgaagagcgcgttcaaaatcatgcatgagatcaccggctccaaagaggatttcattgttgccgatcacacccaaatgaataccccgattggtggtggtccggtgcacgtgccggagtaccaccacattacgtatcatgttaccctgtctaaagacgtcaccgatcaccgtgaccatttgaacattgttgaggtgatcaaggcagttgacctggagacgtaccgttaataa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K914003_sequence
1
ccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaa
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1565001_sequence
1
ccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaatactagatgagatcctcacataagcgtaatttggctgcgctgctttccatcagcggagccgcggtggcacagcagacggcatggggtcaatgcggaggacaaggatggtccggtgcgacatcatgtgtgtcaggctacacttgctcctacacaaatgactggtactcccagtgcgttcctggatcatccggcggcggaggtagcaaccctacaacgaccaccacgatgatccggacgaccacgacggcttcctcctcgggccccaccggcggcgctggtggtggtaaggttcgatttgccggtgtcaacatcgccggctttgatttcggagtggtgatcagcggcactcaagacatgacccaaattgtggacgagtccgtcgatggtgtcaatcagatgcgacactttgtcaacgacgacggcttcaacatcttccgcctcccgtcaggctggcagttcctggtgaacaacaacctcggtggctcactggactcgaacaatttcgccaagtacgacaagctggttcaaggatgtctgtccctgggagcatactgtatcgtcgatgtccacaattacgctcgctggaacggcggcgtcatcggacaaggcggtcccaccgacgaccagttcaccagtctctggactcaactggccactcactacaagagtgaaagcaagatcatctttggtgtgatgaacgagccacacgacctggacatcaaccgatgggccaccaccgtgcaaaaggccgtgacggcgatccgcaaggccggcgccacttcccaaatgatccttcttccaggtaccgacttcaccagtgcggccaactttgtcgagaatggctccggtgccgcgctctcggccgtcaccaacctggacggatccaccaccaacctgatcttcgacgtgcacaagtatctcgactccgataacagcggcacccacgccgagtgcgtgaccaacaacgccgacgctttcaacagcctggcccaatggctccgcaccaacaagcgtcaagccatgctcaccgagaccggtggtggtaacgtccagagctgcggaacatacatgtgccagcaactggatgtgctcaaccaaaactctgacgtttaccttggatggacctcgtggagcgctggaggtttccaggtttcctggaactatgtgctgggtgaggtcccaaccaacaacgttgatacctacctggtcaagcaatgctttgttcccaagtggaagaactactagagcgcgccccgcgattcgctaaggtgctgttgtgtcagtgataagccatccgggactgatggcggaggatgcgcatcgtcgggaaactgatgcctactagagatctatgaccctggataaagcgctggtgctgcgcacttgtgccaataatatggccgaccattgtggcctgatctggcctgcttctggtacagttgaaagccgctattggcagtctacacgtcgtcacgaaaacgggctggttgggctgctgtggggtgccggaacatccgcctttctgagcgttcatgccgatgctcgttggattgtttgtgaggttgctgttgccgacatcattagcctggaagaaccgggtatggtgaaatttcctcgtgctgaagtggtccatgttggtgatcgtattagcgcctcccatttcatttcagctcgtcaggctgatccagcaagcacatcaacaagcacctctaccagtacactgacaccaatgccaaccgctatccctactcctatgccggctgtagcctctgttactctgcctgtggccgaacaagctcgccacgaagtctttgacgttgcctctgtgtctgctgctgctgctcctgttaacactctgcctgttaccacgcctcaaaatctgcaaacggcgacctatggctcaacactgagtggtgataaccattctcgtctgattgccgggtatggctctaacgaaaccgccggaaaccattctgatctgatcggtggtcacgattgtacgctgatggcaggtgatcaatctcgtctgaccgctggcaaaaattccgttctgaccgctggggcacgttctaaactgatcgggagcgaaggctctactctgtccgccggagaagattctaccctgatctttcgtctgtgggatggtaaacgttatcgtcagctggtcgctcgtacaggtgaaaatggtgtggaagccgacattccgtattatgtgaatgaggacgatgatattgtggacaaaccggacgaagatgatgattggatcgaagtgaaaggtactagatgacggcactgactgaaggcgcaaaactgttcgagaaagaaatcccatatatcactgagctggaaggtgacgttgaaggtatgaagtttatcatcaagggtgaaggtaccggtgacgcgagcgtcggtaaagtggatgctcagttcatttgtaccacgggcgacgttccggttccgtggagcacgctggtcaccacgctgacgtatggtgctcagtgctttgccaagtatccgcgccacattgcggatttcttcaaaagctgcatgccggaaggttacgtccaagagcgcaccatcacctttgagggtgatggcgtgttcaagacccgtgcggaagtcacctttgaaaatggcagcgtgtacaaccgtgtaaaactgaacggccagggtttcaagaaggacggccacgtgctgggcaaaaatctggagtttaactttacccctcattgtttgtacatttggggtgaccaagcgaatcatggcctgaagagcgcgttcaaaatcatgcatgagatcaccggctccaaagaggatttcattgttgccgatcacacccaaatgaataccccgattggtggtggtccggtgcacgtgccggagtaccaccacattacgtatcatgttaccctgtctaaagacgtcaccgatcaccgtgaccatttgaacattgttgaggtgatcaaggcagttgacctggagacgtaccgttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z