BBa_K157005
1
BBa_K157005
Split-Cerulean-cCFP
2008-10-25T11:00:00Z
2015-05-08T01:10:54Z
Gene synthesis by GeneArt, optimized for expression in homo sapiens.
C-terminal fragment (Amino acids156-236) of the monomeric[5] cyan fluorescent protein variant ???Cerulean???, designed for fusion/BiFC[1,3]. Reassembly with Split-Cerulean-nCFP (Part Bba_K157006) generates the complete, working CFP ???Cerulean???, whereas combination with the N-terminal fragment (Part Bba_K157008) of the monomeric yellow fluorescent Protein ???Venus???[4] (Part Bba_I757008) results in green fluorescence[2].References see "Part Design".
false
false
_232_
0
1673
9
It's complicated
false
Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
false
iGEM Team Freiburg 2008
annotation2064705
1
C-Terminal portion of cerulean CFP
range2064705
1
1
243
BBa_K157005_sequence
1
aagaacggcatcaaggccaacttcaagatccggcacaacatcgaggatggcagcgtgcagctggccgatcactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagagcaagctgtccaaggaccccaacgagaagcgggaccacatggtgctgctggaatttgtgacagccgccggaatcaccctgggcatggacgagctgtacaag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z