LOCUS BBa_K157041 3425 bp DNA linear 26-Dec-2024 DEFINITION Transfection vector (similar to pSB1AK8) ACCESSION BBa_K157041 VERSION BBa_K157041.1 FEATURES Location/Qualifiers stem_loop 21..257 /label=Eukaryotic terminator misc_feature 1..21 /label=BioBrick suffix (missing a C) stem_loop 267..301 /label=Stem loop primer_bind 394..413 /label=VR misc_feature 498..1196 /label=rep (pMB1) CDS 1334..2149 /label=kanamycin resistance marker CDS 2304..3164 /label=ampicillin resistance marker primer_bind 3207..3226 /label=VF2 misc_feature 3405..3425 /label=BioBrick prefix (missing a 3' G) ORIGIN 1 tactagtagc ggccgctgca gctagagctc gctgatcagc ctcgactgtg ccttctagtt 61 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc 121 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt 181 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca 241 ggcatgctgg ggatatgcac cggcaaaaaa acgggcaagg tgtcaccacc ctgccctttt 301 tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg 361 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 421 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 481 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 541 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 601 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 661 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 721 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 781 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 841 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 901 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 961 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 1021 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 1081 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 1141 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 1201 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 1261 tggtctgaca gctcgagtcc cgtcaagtca gcgtaatgct ctgccagtgt tacaaccaat 1321 taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat 1381 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac 1441 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa 1501 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac 1561 catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt 1621 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat 1681 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac 1741 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac 1801 ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga 1861 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt 1921 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc 1981 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac 2041 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg 2101 aatttaatcg cggcctggag caagacgttt cccgttgaat atggctcata acaccccttg 2161 tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg 2221 caatgtaaca tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg 2281 ctgagttgaa ggatcagctc gagttaccaa tgcttaatca gtgaggcacc tatctcagcg 2341 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 2401 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 2461 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 2521 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 2581 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 2641 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 2701 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 2761 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 2821 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 2881 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 2941 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 3001 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 3061 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 3121 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 3181 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 3241 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 3301 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat 3361 ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc gcggccgctt 3421 ctaga //