BBa_K1582005 1 BBa_K1582005 GFP+sJanus-m Fusion Protein 2015-08-05T11:00:00Z 2015-09-16T08:08:20Z GFP comes frome iGEM's standard part. We construct the mutant of HFBI ourselves, and the original HFBI comes from Trichoderma reesei. HFBI is a modle of class I of hydrophobins, which are naturally produced by filamentous fungi. We use serine, which is similar with cysteine in structure, to replace all of its conserved eight-cysteine residues and get the mutant of HFBI. Hydrophobins are amphipathic, which means they have the hydrophobic part and hydropholic part at the same time. HFBI is a kind of hydrophobins and it has magic properties. We combine GFP with HFBI by a linker and make them become a fusion protein. This fusion protein could be used in aqueous two-phase systems to make itself seperated frome mixture. Meanwile, hydrophobin has a property that it can generate a membrane on the surface of solid automatically, which is called self-assembly. We can get a fluorescent protein' membrane by it. false false _1998_ 23270 27735 9 false When amplify the HFBI and GFP with PCR, temperature is important. false Chenmao Wang component2463064 1 BBa_K1582036 component2463063 1 BBa_K1582032 annotation2463064 1 BBa_K1582036 range2463064 1 715 1017 annotation2463063 1 BBa_K1582032 range2463063 1 1 714 BBa_K1582032 1 BBa_K1582032 GFP without stop codon 2015-09-15T11:00:00Z 2015-09-15T11:28:02Z From BBa_E0040. This part is for making GFP+Janus fusion protein. false false _1998_ 23270 23270 9 false We delete the TAATAA stop codon. false Dongqi Bao BBa_K1582036 1 BBa_K1582036 Linker+sJanus-m 2015-09-15T11:00:00Z 2015-09-15T11:46:51Z From the pET plasmid provided by our instructor. This part is for making fusion protein with sJanus-m. The linker is GGGGSGGGGS. false false _1998_ 23270 23270 9 false Null. false Dongqi Bao BBa_K1582032_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaa BBa_K1582005_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaacctactctgacaaccgttacggtggtacctgcgacccggacggttgcgactggaacccgtaccgtctgggtaacagcaacggcaacggcaatgtttctcctcccggcctcttcagcaacccccagtcttctgccacccaagtccttggcctcatcggccttgactctaaagtcccctcccagaacgtttacgacggcaccgacttccgcaacgtctctgccaaaaccggcgcccagcctctctcttctgtggcccccgttgccggccaggctcttctgtctcagaccgccgtcggtgcttga BBa_K1582036_sequence 1 acctactctgacaaccgttacggtggtacctgcgacccggacggttgcgactggaacccgtaccgtctgggtaacagcaacggcaacggcaatgtttctcctcccggcctcttcagcaacccccagtcttctgccacccaagtccttggcctcatcggccttgactctaaagtcccctcccagaacgtttacgacggcaccgacttccgcaacgtctctgccaaaaccggcgcccagcctctctcttctgtggcccccgttgccggccaggctcttctgtctcagaccgccgtcggtgcttga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z