BBa_K1582007 1 BBa_K1582007 GFP+inJanus-m Fusion Protein 2015-08-06T11:00:00Z 2015-09-16T08:13:59Z It come from some genomic sequence. mHGFI is a small protein produced by filamentous fungi, which use serine to replace all of HGFI's conserved eight-cysteine residues.Hydrophobins are amphipathic, which means they have the hydrophobic part and hydrophilic part at the same time. mHGFI is a kind of hydrophobins and it has magic properties. We combine GFP with mHGFI by a linker and make them become a fusion protein. This fusion protein could be used in aqueous two-phase systems to make itself seperated frome mixture. Meanwile, hydrophobin has a property that it can generate a membrane on the surface of solid automatically, which is called self-assembly. We can get a fluorescent protein' membrane by it. false false _1998_ 23270 27746 9 false We combine GFP with mHGFI by a linker and make them become a fusion protein. false Yuwei Zhang component2463081 1 BBa_K1582035 component2463080 1 BBa_K1582032 annotation2463081 1 BBa_K1582035 range2463081 1 715 1041 annotation2463080 1 BBa_K1582032 range2463080 1 1 714 BBa_K1582032 1 BBa_K1582032 GFP without stop codon 2015-09-15T11:00:00Z 2015-09-15T11:28:02Z From BBa_E0040. This part is for making GFP+Janus fusion protein. false false _1998_ 23270 23270 9 false We delete the TAATAA stop codon. false Dongqi Bao BBa_K1582035 1 BBa_K1582035 Linker+inJanus-m 2015-09-15T11:00:00Z 2015-09-15T11:44:39Z From the pET plasmid provided by our instructor. This part is for making fusion protein with inJanus-m. The linker is GGGGSGGGGS. false false _1998_ 23270 23270 9 false Null. false Dongqi Bao BBa_K1582032_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaa BBa_K1582007_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaacctactctgacaaccgttacggtggtacctgcgacccggacggttgcgactggaacccgtaccgtctgggtaaccaacagtctaccactggccagctccagtcttctgagtctacctccactgcgaacgacccggccaccagcgagctcctcggtctgatcggcgtcgtcatctctgatgtcgacgcactcgtcggtctcacctcttcgccgatctccgtcatcggcgttggcagtggctctgcgtctaccgcgaacccagtgtcttctgactcgtcgcccattggtggactcgtctccatcggatctgttccggttaacgtctga BBa_K1582035_sequence 1 acctactctgacaaccgttacggtggtacctgcgacccggacggttgcgactggaacccgtaccgtctgggtaaccaacagtctaccactggccagctccagtcttctgagtctacctccactgcgaacgacccggccaccagcgagctcctcggtctgatcggcgtcgtcatctctgatgtcgacgcactcgtcggtctcacctcttcgccgatctccgtcatcggcgttggcagtggctctgcgtctaccgcgaacccagtgtcttctgactcgtcgcccattggtggactcgtctccatcggatctgttccggttaacgtctga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z