BBa_K1582038 1 BBa_K1582038 FsC without stop codon 2015-09-15T11:00:00Z 2015-09-15T11:54:34Z From BBa_K808025. This part is for making FsC+Janus fusion protein. false false _1998_ 23270 23270 9 false We delete the TAATAA stop codon. false Dongqi Bao BBa_K1582021 1 BBa_K1582021 FsC+sJanus-m Fusion Protein 2015-08-06T11:00:00Z 2015-09-16T08:41:49Z Get sequence from BBa_K808025 This part bases on BBa_K1582020. For enhancing success rate and expression of fusion protein, we mutate of eight Cys to Ser because of their resemble structure. After doing some similar experiment, we can ensure the function of mutation that whether it can better express and fold in E. coli and would it have the same function for hydrolyzing PET false false _1998_ 23270 27738 9 false Mutate of eight Cys to Ser because of their resemble structure. false LIU JIASHU component2463329 1 BBa_K1582036 component2463328 1 BBa_K1582038 annotation2463328 1 BBa_K1582038 range2463328 1 1 645 annotation2463329 1 BBa_K1582036 range2463329 1 646 948 BBa_K1582036 1 BBa_K1582036 Linker+sJanus-m 2015-09-15T11:00:00Z 2015-09-15T11:46:51Z From the pET plasmid provided by our instructor. This part is for making fusion protein with sJanus-m. The linker is GGGGSGGGGS. false false _1998_ 23270 23270 9 false Null. false Dongqi Bao BBa_K1582021_sequence 1 atgctgcctacttctaaccctgctcaggagcttgaggcgcgccagcttggtagaacaactcgcgacgatctgatcaacggcaatagcgcttcctgcgccgatgtcatcttcatttatgcccggggttcaacagagacgggcaacttgggaactctcggtcctagcattgcctccaaccttgagtccgccttcggcaaggacggtgtctggattcagggcgttggcggtgcctaccgagccactcttggagacaatgctctccctcgcggtacctctagcgccgcaatcagggagatgcttggtctcttccagcaggccaacaccaagtgccctgacgcgactttgatcgccggtggctacagccagggtgctgcacttgcggccgcctccatcgaggacctcgactcggccattcgtgacaagatcgccggaactgttctgttcggctacaccaagaacctacagaaccgtggccgaatccccaactaccctgccgacaggaccaaggtcttctgcaatacaggagatctcgtttgtactggtagcttgatcgttgctgcacctcacttggcttatggtcctgatgctcggggccctgcccctgagttcctcatcgagaaggttcgggctgtccgtggttctgctacctactctgacaaccgttacggtggtacctgcgacccggacggttgcgactggaacccgtaccgtctgggtaacagcaacggcaacggcaatgtttctcctcccggcctcttcagcaacccccagtcttctgccacccaagtccttggcctcatcggccttgactctaaagtcccctcccagaacgtttacgacggcaccgacttccgcaacgtctctgccaaaaccggcgcccagcctctctcttctgtggcccccgttgccggccaggctcttctgtctcagaccgccgtcggtgcttga BBa_K1582038_sequence 1 atgctgcctacttctaaccctgctcaggagcttgaggcgcgccagcttggtagaacaactcgcgacgatctgatcaacggcaatagcgcttcctgcgccgatgtcatcttcatttatgcccggggttcaacagagacgggcaacttgggaactctcggtcctagcattgcctccaaccttgagtccgccttcggcaaggacggtgtctggattcagggcgttggcggtgcctaccgagccactcttggagacaatgctctccctcgcggtacctctagcgccgcaatcagggagatgcttggtctcttccagcaggccaacaccaagtgccctgacgcgactttgatcgccggtggctacagccagggtgctgcacttgcggccgcctccatcgaggacctcgactcggccattcgtgacaagatcgccggaactgttctgttcggctacaccaagaacctacagaaccgtggccgaatccccaactaccctgccgacaggaccaaggtcttctgcaatacaggagatctcgtttgtactggtagcttgatcgttgctgcacctcacttggcttatggtcctgatgctcggggccctgcccctgagttcctcatcgagaaggttcgggctgtccgtggttctgct BBa_K1582036_sequence 1 acctactctgacaaccgttacggtggtacctgcgacccggacggttgcgactggaacccgtaccgtctgggtaacagcaacggcaacggcaatgtttctcctcccggcctcttcagcaacccccagtcttctgccacccaagtccttggcctcatcggccttgactctaaagtcccctcccagaacgtttacgacggcaccgacttccgcaacgtctctgccaaaaccggcgcccagcctctctcttctgtggcccccgttgccggccaggctcttctgtctcagaccgccgtcggtgcttga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z