BBa_K1582025 1 BBa_K1582025 Thc_Cut1+sJanus-m Fusion Protein 2015-09-07T11:00:00Z 2015-09-16T09:58:18Z Thc_Cut1+mHFBI Thc_Cut1+mHFBI false false _1998_ 23270 23270 9 false Thc_Cut1+mHFBI false Dongqi Bao component2463710 1 BBa_K1582039 component2463711 1 BBa_K1582034 annotation2463710 1 BBa_K1582039 range2463710 1 1 786 annotation2463711 1 BBa_K1582034 range2463711 1 787 1089 BBa_K1582034 1 BBa_K1582034 Linker+sJanus 2015-09-15T11:00:00Z 2015-09-15T11:42:30Z From the pET plasmid provided by our instructor. This part is for making fusion protein with sJanus. The linker is GGGGSGGGGS. false false _1998_ 23270 23270 9 false Null. false Dongqi Bao BBa_K1582039 1 BBa_K1582039 Thc_Cut1 without stop codon 2015-09-15T11:00:00Z 2015-09-15T11:57:59Z We delete the TGA stop codon. This part is for making Thc_Cut1+Janus fusion protein. false false _1998_ 23270 23270 9 false From IDT gBlock fragment synthesis. false Dongqi Bao BBa_K1582025_sequence 1 atggctaatccatacgagcggggtccaaatccgacggatgcgctgttagaagcgtcttccggtcctttctccgttagtgaagaaaatgttagtcgtctgagcgcctccggctttgggggcggcaccatctattacccccgtgaaaataatacctatggcgcggtggctatttcgccgggctataccggcacggaggcttctatcgcatggctcggcgagcggatcgcttcgcatgggtttgttgttattacaatcgacaccattaccactctggatcagccggactcacgcgccgagcaactgaacgcagcccttaaccatatgattaatcgtgcctcctcaacagtccgctcacgcattgatagtagccggttagcagtaatggggcatagcatgggcggcggtggtacattacgtttggcgtcacagcgtccggacttaaaagcggcgatcccgctgaccccgtggcatctgaataaaaattggtccagcgtgacggtccccactttaattatcggagcggatttagataccatcgcgccggtcgccacccacgcgaaaccgttctataacagcctcccctcgtccatttcaaaggcgtatcttgaacttgatggtgcgacccatttcgcaccgaatattcccaacaagattatcggcaagtacagcgtggcttggctgaagcgtttcgtggataatgatacgcgctacactcagttcttgtgtcccgggccgcgtgatggccttttcggagaagttgaagaatatcgttcgacgtgtccgttcacctactctgacaaccgttacggtggtacctgcgacccggacggttgcgactggaacccgtaccgtctgggtaacagcaacggcaacggcaatgtttgccctcccggcctcttcagcaacccccagtgctgtgccacccaagtccttggcctcatcggccttgactgcaaagtcccctcccagaacgtttacgacggcaccgacttccgcaacgtctgcgccaaaaccggcgcccagcctctctgctgcgtggcccccgttgccggccaggctcttctgtgccagaccgccgtcggtgcttga BBa_K1582039_sequence 1 atggctaatccatacgagcggggtccaaatccgacggatgcgctgttagaagcgtcttccggtcctttctccgttagtgaagaaaatgttagtcgtctgagcgcctccggctttgggggcggcaccatctattacccccgtgaaaataatacctatggcgcggtggctatttcgccgggctataccggcacggaggcttctatcgcatggctcggcgagcggatcgcttcgcatgggtttgttgttattacaatcgacaccattaccactctggatcagccggactcacgcgccgagcaactgaacgcagcccttaaccatatgattaatcgtgcctcctcaacagtccgctcacgcattgatagtagccggttagcagtaatggggcatagcatgggcggcggtggtacattacgtttggcgtcacagcgtccggacttaaaagcggcgatcccgctgaccccgtggcatctgaataaaaattggtccagcgtgacggtccccactttaattatcggagcggatttagataccatcgcgccggtcgccacccacgcgaaaccgttctataacagcctcccctcgtccatttcaaaggcgtatcttgaacttgatggtgcgacccatttcgcaccgaatattcccaacaagattatcggcaagtacagcgtggcttggctgaagcgtttcgtggataatgatacgcgctacactcagttcttgtgtcccgggccgcgtgatggccttttcggagaagttgaagaatatcgttcgacgtgtccgttc BBa_K1582034_sequence 1 acctactctgacaaccgttacggtggtacctgcgacccggacggttgcgactggaacccgtaccgtctgggtaacagcaacggcaacggcaatgtttgccctcccggcctcttcagcaacccccagtgctgtgccacccaagtccttggcctcatcggccttgactgcaaagtcccctcccagaacgtttacgacggcaccgacttccgcaacgtctgcgccaaaaccggcgcccagcctctctgctgcgtggcccccgttgccggccaggctcttctgtgccagaccgccgtcggtgcttga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z