BBa_K1583013
1
BBa_K1583013
CsgA missing the start codon for fusion protein
2015-09-03T11:00:00Z
2015-09-18T06:57:49Z
This DNA was synthesized. The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1]
1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.
An illegal PstI restriction site (766) in CsgA had to be mutated (A->G) not changing the protein sequence.
In order to create a fusion protein of CsgA with Mfp5 in front of it, CsgA was designed without its start codon.
false
false
_2000_
24478
24463
9
false
An illegal PstI restriction site (766) in CsgA had to be mutated (A->G) not changing the protein sequence.
false
Stefan Robert Marsden
annotation2474838
1
CsgA
range2474838
1
1
393
BBa_K1583013_sequence
1
ggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgctgcggttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z