BBa_K1583018
1
BBa_K1583018
SpyCatcher without startcodon + His-tag
2015-09-13T11:00:00Z
2015-09-14T01:44:54Z
This gene was synthesized as part of biobrick BBa_K1583113. The idea of using Spytag/SpyCatcher was based on the paper "Programmable biofilm-based materials fromengineered curli nanofibres"[1]
1. Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.
SpyCatcher is a protein that can spontaneously form covalent isopeptide linkages with it??s counterpart `Spytag` under physiological conditions.
false
false
_2000_
24478
24478
9
false
Startcodon was not included, since BFP (BBa_K1583017) was placed in front of this biobrick.
false
Max van 't Hof
annotation2453887
1
His-tag
range2453887
1
388
405
annotation2453886
1
SpyCatcher
range2453886
1
1
387
annotation2453888
1
Stop-codon
range2453888
1
406
408
BBa_K1583018_sequence
1
gattacgacatcccaacgaccgaaaacctgtattttcagggcgccatggttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatgacaattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatattcatcatcaccatcaccactaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z