LOCUS       BBa_K1583104             850 bp    DNA     linear       29-Nov-2024
DEFINITION  pRha + Mfp5_CsgA_His fusion protein
ACCESSION   BBa_K1583104
VERSION     BBa_K1583104.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=Scar
     misc_feature    1..6
                     /label=BBa_K1583054
     promoter        9..130
                     /label=pRha
     promoter        9..130
                     /label=BBa_K914003
     misc_feature    131..157
                     /label=Spacer
     misc_feature    131..157
                     /label=BBa_K1583056
     RBS             158..169
                     /label=RBS
     RBS             158..169
                     /label=BBa_K1583061
     CDS             170..400
                     /label=Mfp5
     CDS             170..400
                     /label=BBa_K1583002
     misc_feature    401..430
                     /label=Linker
     misc_feature    401..430
                     /label=BBa_K1583012
     CDS             431..823
                     /label=CsgA
     CDS             431..823
                     /label=BBa_K1583013
     misc_feature    824..844
                     /label=His-tag
     CDS             824..844
                     /label=BBa_K1583059
     misc_feature    845..850
                     /label=Scar
     misc_feature    845..850
                     /label=BBa_K1583057
ORIGIN
        1 tactagagcc acaattcagc aaattgtgaa catcatcacg ttcatctttc cctggttgcc
       61 aatggcccat tttcctgtca gtaacgagaa ggtcgcgtat tcaggcgctt tttagactgg
      121 tcgtaatgaa atactagagc agcaaggaaa tactagaaaa gaggagaaaa tgagttctga
      181 agaatacaaa ggtggttatt acccaggcaa tacttaccac tatcattcag gtggtagtta
      241 tcacggatcc ggctatcatg gaggatataa gggaaagtat tacggaaagg caaagaaata
      301 ctattataaa tataaaaaca gcggaaaata caagtatctg aagaaagcta gaaaatacca
      361 tagaaagggt tacaagaagt attatggagg tggtagcagt ggcggtggcg gtagcggtgg
      421 cggtggcagt ggtgttgttc ctcagtacgg cggcggcggt aaccacggtg gtggcggtaa
      481 taatagcggc ccaaattctg agctgaacat ttaccagtac ggtggcggta actctgcact
      541 tgctctgcaa actgatgccc gtaactctga cttgactatt acccagcatg gcggcggtaa
      601 tggtgcagat gttggtcagg gctcagatga cagctcaatc gatctgaccc aacgtggctt
      661 cggtaacagc gctactcttg atcagtggaa cggcaaaaat tctgaaatga cggttaaaca
      721 gttcggtggt ggcaacggtg ctgcggttga ccagactgca tctaactcct ccgtcaacgt
      781 gactcaggtt ggctttggta acaacgcgac cgctcatcag taccatcacc atcaccatca
      841 ctaaggtacc
//