BBa_K1583104
type: ComponentDefinition
persistent identity: BBa K1583104
display id: BBa_K1583104
version: 1
was generated by: iGEM to SBOL conversion
description: pRha + Mfp5_CsgA_His fusion protein
created: 2015-09-03T11:00:00Z
modified: 2015-09-18T03:37:06Z
mutable provenance: This part originates from the E.coli K-12 MG1655 genome and from mussels. The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1] 1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.
owned by: james
owned by: myers
mutable description: CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms taken from E.coli K-12 MG1655. Inspired by mussels, the Mfp5 (mussel foot protein) has high adhesive properties towards wet polar surfaces. By creating a fusion protein, the adhesive properties of the mussel foot protein is combined with the formation of nanowires. This DNA was synthesized. The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1] 1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866. The CsgA sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).
discontinued: false
dominant: false
experience: experience/Works
group u list: _2000_
m user id: 24478
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: A mutation had to be introduced at base pair 766 to remove a PstI restriction site. The protein sequence was not changed in doing so.
star: false
creator: Stefan Robert Marsden
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_K1583054
component: BBa_K1583056
component: BBa_K914003
component: BBa_K1583002
component: BBa_K1583061
component: BBa_K1583013
component: BBa_K1583012
component: BBa_K1583057
component: BBa_K1583059
sequence annotation: BBa_K1583054
sequence annotation: BBa_K914003
sequence annotation: BBa_K1583056
sequence annotation: BBa_K1583061
sequence annotation: BBa_K1583002
sequence annotation: BBa_K1583012
sequence annotation: BBa_K1583013
sequence annotation: BBa_K1583059
sequence annotation: BBa_K1583057
sequence: https://synbiohub.org/public/igem/BBa_K1583104_sequence/1

BBa_K1583054
type: Component
persistent identity: component2461119
display id: component2461119
version: 1
top level: BBa_K1583104
definition: BBa_K1583054
access: public

BBa_K1583056
type: Component
persistent identity: component2461122
display id: component2461122
version: 1
top level: BBa_K1583104
definition: BBa_K1583056
access: public

BBa_K914003
type: Component
persistent identity: component2461121
display id: component2461121
version: 1
top level: BBa_K1583104
definition: BBa_K914003
access: public

BBa_K1583002
type: Component
persistent identity: component2461124
display id: component2461124
version: 1
top level: BBa_K1583104
definition: BBa_K1583002
access: public

BBa_K1583061
type: Component
persistent identity: component2461123
display id: component2461123
version: 1
top level: BBa_K1583104
definition: BBa_K1583061
access: public

BBa_K1583013
type: Component
persistent identity: component2461126
display id: component2461126
version: 1
top level: BBa_K1583104
definition: BBa_K1583013
access: public

BBa_K1583012
type: Component
persistent identity: component2461125
display id: component2461125
version: 1
top level: BBa_K1583104
definition: BBa_K1583012
access: public

BBa_K1583057
type: Component
persistent identity: component2461128
display id: component2461128
version: 1
top level: BBa_K1583104
definition: BBa_K1583057
access: public

BBa_K1583059
type: Component
persistent identity: component2461127
display id: component2461127
version: 1
top level: BBa_K1583104
definition: BBa_K1583059
access: public

BBa_K1583054
type: SequenceAnnotation
persistent identity: annotation2461119
display id: annotation2461119
version: 1
top level: BBa_K1583104
location: https://synbiohub.org/public/igem/BBa_K1583104/annotation2461119/range2461119/1
component: BBa_K1583054

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persistent identity: range2461119
display id: range2461119
version: 1
top level: BBa_K1583104
direction: direction/reverse
start: 1
end: 6
orientation: inline

BBa_K914003
type: SequenceAnnotation
persistent identity: annotation2461121
display id: annotation2461121
version: 1
top level: BBa_K1583104
location: https://synbiohub.org/public/igem/BBa_K1583104/annotation2461121/range2461121/1
component: BBa_K914003

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persistent identity: range2461121
display id: range2461121
version: 1
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direction: direction/reverse
start: 9
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BBa_K1583056
type: SequenceAnnotation
persistent identity: annotation2461122
display id: annotation2461122
version: 1
top level: BBa_K1583104
location: https://synbiohub.org/public/igem/BBa_K1583104/annotation2461122/range2461122/1
component: BBa_K1583056

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type: Range
persistent identity: range2461122
display id: range2461122
version: 1
top level: BBa_K1583104
direction: direction/reverse
start: 131
end: 157
orientation: inline

BBa_K1583061
type: SequenceAnnotation
persistent identity: annotation2461123
display id: annotation2461123
version: 1
top level: BBa_K1583104
location: https://synbiohub.org/public/igem/BBa_K1583104/annotation2461123/range2461123/1
component: BBa_K1583061

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type: Range
persistent identity: range2461123
display id: range2461123
version: 1
top level: BBa_K1583104
direction: direction/reverse
start: 158
end: 169
orientation: inline

BBa_K1583002
type: SequenceAnnotation
persistent identity: annotation2461124
display id: annotation2461124
version: 1
top level: BBa_K1583104
location: https://synbiohub.org/public/igem/BBa_K1583104/annotation2461124/range2461124/1
component: BBa_K1583002

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type: Range
persistent identity: range2461124
display id: range2461124
version: 1
top level: BBa_K1583104
direction: direction/reverse
start: 170
end: 400
orientation: inline

BBa_K1583012
type: SequenceAnnotation
persistent identity: annotation2461125
display id: annotation2461125
version: 1
top level: BBa_K1583104
location: https://synbiohub.org/public/igem/BBa_K1583104/annotation2461125/range2461125/1
component: BBa_K1583012

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type: Range
persistent identity: range2461125
display id: range2461125
version: 1
top level: BBa_K1583104
direction: direction/reverse
start: 401
end: 430
orientation: inline

BBa_K1583013
type: SequenceAnnotation
persistent identity: annotation2461126
display id: annotation2461126
version: 1
top level: BBa_K1583104
location: https://synbiohub.org/public/igem/BBa_K1583104/annotation2461126/range2461126/1
component: BBa_K1583013

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type: Range
persistent identity: range2461126
display id: range2461126
version: 1
top level: BBa_K1583104
direction: direction/reverse
start: 431
end: 823
orientation: inline

BBa_K1583059
type: SequenceAnnotation
persistent identity: annotation2461127
display id: annotation2461127
version: 1
top level: BBa_K1583104
location: https://synbiohub.org/public/igem/BBa_K1583104/annotation2461127/range2461127/1
component: BBa_K1583059

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type: Range
persistent identity: range2461127
display id: range2461127
version: 1
top level: BBa_K1583104
direction: direction/reverse
start: 824
end: 844
orientation: inline

BBa_K1583057
type: SequenceAnnotation
persistent identity: annotation2461128
display id: annotation2461128
version: 1
top level: BBa_K1583104
location: https://synbiohub.org/public/igem/BBa_K1583104/annotation2461128/range2461128/1
component: BBa_K1583057

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type: Range
persistent identity: range2461128
display id: range2461128
version: 1
top level: BBa_K1583104
direction: direction/reverse
start: 845
end: 850
orientation: inline

BBa_K1583054
type: ComponentDefinition
persistent identity: BBa K1583054
display id: BBa_K1583054
version: 1
was generated by: iGEM to SBOL conversion
description: Non-coding scar site in between RBS and gene
created: 2015-09-03T11:00:00Z
modified: 2015-09-18T08:03:55Z
mutable provenance: The RBS and CsgC were synthesized from the genomic sequence of E.coli K-12 MG1655. The promoter is part of the constitutive promoter family made by John Anderson from iGEM Berkeley 2006.
owned by: james
owned by: myers
mutable description: CsgC is positively involved in the extracellular aggregation of CsgA into amyloid nanowires (curli assembly) in E.coli functioning as a chaperone for CsgA.
discontinued: false
dominant: false
group u list: _2000_
m user id: 24478
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We inserted non-coding scar sequences after the promoter and RBS to prevent missing initial codons during transcription/translation.
star: false
creator: Stefan Robert Marsden
type: DnaRegion
role: partType/Scar
role: SO:0001953
sequence annotation: Scar
sequence: https://synbiohub.org/public/igem/BBa_K1583054_sequence/1

Scar
type: SequenceAnnotation
persistent identity: annotation2475152
display id: annotation2475152
version: 1
top level: BBa_K1583054
location: https://synbiohub.org/public/igem/BBa_K1583054/annotation2475152/range2475152/1
role: feature/scar

https://synbiohub.org/public/igem/BBa_K1583054/annotation2475152/range2475152/1
type: Range
persistent identity: range2475152
display id: range2475152
version: 1
top level: BBa_K1583054
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_K1583002
type: ComponentDefinition
persistent identity: BBa K1583002
display id: BBa_K1583002
version: 1
was generated by: iGEM to SBOL conversion
description: Mfp5 adhesive protein
created: 2015-09-03T11:00:00Z
modified: 2015-09-18T06:35:39Z
mutable provenance: This DNA was synthesized. The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1] 1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C.Stultz, T. Lu, Nature Nanotechnology 2014, 9, 858-866.
owned by: james
owned by: myers
mutable description: Inspired by mussels, the Mfp5 (mussel foot protein) has high adhesive properties towards wet polar surfaces.
discontinued: false
dominant: false
group u list: _2000_
m user id: 24478
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No special considerations were required.
star: false
creator: Stefan Robert Marsden
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Mfp5
sequence: https://synbiohub.org/public/igem/BBa_K1583002_sequence/1

Mfp5
type: SequenceAnnotation
persistent identity: annotation2474745
display id: annotation2474745
version: 1
top level: BBa_K1583002
location: https://synbiohub.org/public/igem/BBa_K1583002/annotation2474745/range2474745/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1583002/annotation2474745/range2474745/1
type: Range
persistent identity: range2474745
display id: range2474745
version: 1
top level: BBa_K1583002
direction: direction/forward
start: 1
end: 231
orientation: inline

BBa_K1583012
type: ComponentDefinition
persistent identity: BBa K1583012
display id: BBa_K1583012
version: 1
was generated by: iGEM to SBOL conversion
description: Linker in fusion protein
created: 2015-09-03T11:00:00Z
modified: 2015-09-18T06:53:59Z
mutable provenance: This DNA was synthesized. The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1] 1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.
owned by: james
owned by: myers
mutable description: This amino acid sequence was used as a linker to create a fusion protein from Mfp3/Mfp5 with CsgA.
discontinued: false
dominant: false
group u list: _2000_
m user id: 24478
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No special design considerations needed to be taken.
star: false
creator: Stefan Robert Marsden
type: DnaRegion
role: SO:0001953
role: partType/Scar
sequence annotation: Linker
sequence: https://synbiohub.org/public/igem/BBa_K1583012_sequence/1

Linker
type: SequenceAnnotation
persistent identity: annotation2474770
display id: annotation2474770
version: 1
top level: BBa_K1583012
location: https://synbiohub.org/public/igem/BBa_K1583012/annotation2474770/range2474770/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K1583012/annotation2474770/range2474770/1
type: Range
persistent identity: range2474770
display id: range2474770
version: 1
top level: BBa_K1583012
direction: direction/forward
start: 1
end: 30
orientation: inline

BBa_K1583056
type: ComponentDefinition
persistent identity: BBa K1583056
display id: BBa_K1583056
version: 1
was generated by: iGEM to SBOL conversion
description: Spacer in between promoter and coding sequence
created: 2015-09-03T11:00:00Z
modified: 2015-09-04T06:32:19Z
mutable provenance: This DNA was synthesized. The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1] 1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.
owned by: james
owned by: myers
mutable description: This sequence was designed as a spacer to prevent errors in transcription by placing the RBS too close to the promoter.
discontinued: false
dominant: false
group u list: _2000_
m user id: 24463
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This sequence was designed as a spacer to prevent errors in transcription by placing the RBS too close to the promoter. Formation of a hairpin structure or self complementary sequences was avoided.
star: false
creator: Stefan Robert Marsden
type: DnaRegion
role: SO:0001953
role: partType/Scar
sequence annotation: Spacer
sequence: https://synbiohub.org/public/igem/BBa_K1583056_sequence/1

Spacer
type: SequenceAnnotation
persistent identity: annotation2475156
display id: annotation2475156
version: 1
top level: BBa_K1583056
location: https://synbiohub.org/public/igem/BBa_K1583056/annotation2475156/range2475156/1
role: feature/scar

https://synbiohub.org/public/igem/BBa_K1583056/annotation2475156/range2475156/1
type: Range
persistent identity: range2475156
display id: range2475156
version: 1
top level: BBa_K1583056
direction: direction/forward
start: 1
end: 27
orientation: inline

BBa_K1583057
type: ComponentDefinition
persistent identity: BBa K1583057
display id: BBa_K1583057
version: 1
was generated by: iGEM to SBOL conversion
description: Spacer in between coding sequence and suffix
created: 2015-09-03T11:00:00Z
modified: 2015-09-04T06:40:05Z
mutable provenance: Non-coding and non-self-complementary codons were chosen at random.
owned by: james
owned by: myers
mutable description: Designed as a spacer of 6 base pairs between our coding sequence and the biobrick suffix.
discontinued: false
dominant: false
group u list: _2000_
m user id: 24463
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Hairpin formation and self-complementary sequences were avoided.
star: false
creator: Stefan Robert Marsden
type: DnaRegion
role: partType/Scar
role: SO:0001953
sequence annotation: Scar
sequence: https://synbiohub.org/public/igem/BBa_K1583057_sequence/1

Scar
type: SequenceAnnotation
persistent identity: annotation2475159
display id: annotation2475159
version: 1
top level: BBa_K1583057
location: https://synbiohub.org/public/igem/BBa_K1583057/annotation2475159/range2475159/1
role: feature/scar

https://synbiohub.org/public/igem/BBa_K1583057/annotation2475159/range2475159/1
type: Range
persistent identity: range2475159
display id: range2475159
version: 1
top level: BBa_K1583057
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_K1583013
type: ComponentDefinition
persistent identity: BBa K1583013
display id: BBa_K1583013
version: 1
was generated by: iGEM to SBOL conversion
description: CsgA missing the start codon for fusion protein
created: 2015-09-03T11:00:00Z
modified: 2015-09-18T06:57:49Z
mutable provenance: This DNA was synthesized. The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1] 1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866. An illegal PstI restriction site (766) in CsgA had to be mutated (A->G) not changing the protein sequence.
owned by: james
owned by: myers
mutable description: In order to create a fusion protein of CsgA with Mfp5 in front of it, CsgA was designed without its start codon.
discontinued: false
dominant: false
group u list: _2000_
m user id: 24478
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: An illegal PstI restriction site (766) in CsgA had to be mutated (A->G) not changing the protein sequence.
star: false
creator: Stefan Robert Marsden
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CsgA
sequence: https://synbiohub.org/public/igem/BBa_K1583013_sequence/1

CsgA
type: SequenceAnnotation
persistent identity: annotation2474838
display id: annotation2474838
version: 1
top level: BBa_K1583013
location: https://synbiohub.org/public/igem/BBa_K1583013/annotation2474838/range2474838/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1583013/annotation2474838/range2474838/1
type: Range
persistent identity: range2474838
display id: range2474838
version: 1
top level: BBa_K1583013
direction: direction/forward
start: 1
end: 393
orientation: inline

BBa_K1583059
type: ComponentDefinition
persistent identity: BBa K1583059
display id: BBa_K1583059
version: 1
was generated by: iGEM to SBOL conversion
description: His-tag without start codon
created: 2015-09-03T11:00:00Z
modified: 2015-09-18T11:05:34Z
mutable provenance: Standard DNA sequence optimized for E.coli.
owned by: james
owned by: myers
mutable description: Addition of a His tag at the N-terminal end of a protein of interest is goal of this basic part.
discontinued: false
dominant: false
experience: experience/None
group u list: _2000_
m user id: 24478
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The codon was optimized for synthesis to avoid repetetive motives of too many codons.
star: false
creator: Stefan Robert Marsden
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: His-tag
sequence: https://synbiohub.org/public/igem/BBa_K1583059_sequence/1

His-tag
type: SequenceAnnotation
persistent identity: annotation2473195
display id: annotation2473195
version: 1
top level: BBa_K1583059
location: https://synbiohub.org/public/igem/BBa_K1583059/annotation2473195/range2473195/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1583059/annotation2473195/range2473195/1
type: Range
persistent identity: range2473195
display id: range2473195
version: 1
top level: BBa_K1583059
direction: direction/forward
start: 1
end: 21
orientation: inline

BBa_K1583061
type: ComponentDefinition
persistent identity: BBa K1583061
display id: BBa_K1583061
version: 1
was generated by: iGEM to SBOL conversion
description: RBS
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T07:43:48Z
mutable provenance: The DNA was synthesized. The sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055). The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1] 1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.
owned by: james
owned by: myers
mutable description: This RBS was used in our constructs Mfp5+CsgA and CsgA+Mfp3. CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms taken from E.coli K-12 MG1655. Inspired by mussels, the Mfp5 (mussel foot protein) has high adhesive properties towards wet polar surfaces. This DNA was synthesized. The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1] 1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.
discontinued: false
dominant: false
group u list: _2000_
m user id: 24463
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1] 1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.
star: false
creator: Stefan Robert Marsden
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K1583061_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2475161
display id: annotation2475161
version: 1
top level: BBa_K1583061
location: https://synbiohub.org/public/igem/BBa_K1583061/annotation2475161/range2475161/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1583061/annotation2475161/range2475161/1
type: Range
persistent identity: range2475161
display id: range2475161
version: 1
top level: BBa_K1583061
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_K914003
type: ComponentDefinition
persistent identity: BBa K914003
display id: BBa_K914003
version: 1
was generated by: iGEM to SBOL conversion
description: L-rhamnose-inducible promoter (pRha)
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:45Z
mutable provenance: Amplification of the plasmid pJOE3075 (Dr. Altenbuchner).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: L-rhamnose-inducible promoter is capable of high-level recombinant protein expression in the presence of L-rhamnose, it is also tightly regulated in the absence of L-rhamnose by the addition of D-glucose.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1179_
m user id: 0
owner id: 13487
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: One base pair modified (90: A -> T) to avoid EcoRI site. Mutation made in a less conserved base pair.
star: false
creator: Denis Samuylov
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: pRha
sequence: https://synbiohub.org/public/igem/BBa_K914003_sequence/1

pRha
type: SequenceAnnotation
persistent identity: annotation2188559
display id: annotation2188559
version: 1
top level: BBa_K914003
location: https://synbiohub.org/public/igem/BBa_K914003/annotation2188559/range2188559/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K914003/annotation2188559/range2188559/1
type: Range
persistent identity: range2188559
display id: range2188559
version: 1
top level: BBa_K914003
direction: direction/forward
start: 1
end: 122
orientation: inline

https://synbiohub.org/public/igem/BBa_K1583104_sequence/1
type: Sequence
persistent identity: BBa K1583104 sequence
display id: BBa_K1583104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagagccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaaatactagagcagcaaggaaatactagaaaagaggagaaaatgagttctgaagaatacaaaggtggttattacccaggcaatacttaccactatcattcaggtggtagttatcacggatccggctatcatggaggatataagggaaagtattacggaaaggcaaagaaatactattataaatataaaaacagcggaaaatacaagtatctgaagaaagctagaaaataccatagaaagggttacaagaagtattatggaggtggtagcagtggcggtggcggtagcggtggcggtggcagtggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgctgcggttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtaccatcaccatcaccatcactaaggtacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583054_sequence/1
type: Sequence
persistent identity: BBa K1583054 sequence
display id: BBa_K1583054_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583002_sequence/1
type: Sequence
persistent identity: BBa K1583002 sequence
display id: BBa_K1583002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagttctgaagaatacaaaggtggttattacccaggcaatacttaccactatcattcaggtggtagttatcacggatccggctatcatggaggatataagggaaagtattacggaaaggcaaagaaatactattataaatataaaaacagcggaaaatacaagtatctgaagaaagctagaaaataccatagaaagggttacaagaagtattatggaggtggtagcagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583012_sequence/1
type: Sequence
persistent identity: BBa K1583012 sequence
display id: BBa_K1583012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcggtggcggtagcggtggcggtggcagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583056_sequence/1
type: Sequence
persistent identity: BBa K1583056 sequence
display id: BBa_K1583056_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atactagagcagcaaggaaatactaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583057_sequence/1
type: Sequence
persistent identity: BBa K1583057 sequence
display id: BBa_K1583057_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583013_sequence/1
type: Sequence
persistent identity: BBa K1583013 sequence
display id: BBa_K1583013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgctgcggttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583059_sequence/1
type: Sequence
persistent identity: BBa K1583059 sequence
display id: BBa_K1583059_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catcaccatcaccatcactaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583061_sequence/1
type: Sequence
persistent identity: BBa K1583061 sequence
display id: BBa_K1583061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K914003_sequence/1
type: Sequence
persistent identity: BBa K914003 sequence
display id: BBa_K914003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z