BBa_K1583107
type: ComponentDefinition
persistent identity: BBa K1583107
display id: BBa_K1583107
version: 1
was generated by: iGEM to SBOL conversion
description: pRha + CsgA with His-tag & pTet + RFP
created: 2015-09-07T11:00:00Z
modified: 2016-01-25T11:30:54Z
mutable provenance: The DNA was synthesized. The sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).
owned by: james
owned by: myers
mutable description: Upon induction with rhamnose, CsgA with a N-terminal His tag is expressed. These protein monomers can aggregate to form amyloid nanofibres. In order to better visualize the individual cells in the biofilm, we added the constitutive expression of RFP to our plasmid in a different operon. For our design, we took advantage of the double terminator present in the biobrick I13521 by placing it in front of our genes.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2000_
m user id: 4206
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: For our design, we took advantage of the double terminator present in the biobrick I13521 by placing it in front of our genes.
star: false
creator: Stefan Robert Marsden
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1583101
component: BBa_I13521
sequence annotation: BBa_I13521
sequence annotation: BBa_K1583101
sequence: https://synbiohub.org/public/igem/BBa_K1583107_sequence/1

BBa_K1583101
type: Component
persistent identity: component2446932
display id: component2446932
version: 1
top level: BBa_K1583107
definition: BBa_K1583101
access: public

BBa_I13521
type: Component
persistent identity: component2446922
display id: component2446922
version: 1
top level: BBa_K1583107
definition: BBa_I13521
access: public

BBa_I13521
type: SequenceAnnotation
persistent identity: annotation2446922
display id: annotation2446922
version: 1
top level: BBa_K1583107
location: https://synbiohub.org/public/igem/BBa_K1583107/annotation2446922/range2446922/1
component: BBa_I13521

https://synbiohub.org/public/igem/BBa_K1583107/annotation2446922/range2446922/1
type: Range
persistent identity: range2446922
display id: range2446922
version: 1
top level: BBa_K1583107
direction: direction/reverse
start: 1
end: 923
orientation: inline

BBa_K1583101
type: SequenceAnnotation
persistent identity: annotation2446932
display id: annotation2446932
version: 1
top level: BBa_K1583107
location: https://synbiohub.org/public/igem/BBa_K1583107/annotation2446932/range2446932/1
component: BBa_K1583101

https://synbiohub.org/public/igem/BBa_K1583107/annotation2446932/range2446932/1
type: Range
persistent identity: range2446932
display id: range2446932
version: 1
top level: BBa_K1583107
direction: direction/reverse
start: 932
end: 1591
orientation: inline

BBa_I13521
type: ComponentDefinition
persistent identity: BBa I13521
display id: BBa_I13521
version: 1
was generated by: iGEM to SBOL conversion
description: Ptet mRFP
created: 2005-06-21T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive on mRFP (positive control)
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_R0040
component: BBa_E1010
component: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13521_sequence/1

BBa_B0012
type: Component
persistent identity: component1532589
display id: component1532589
version: 1
top level: BBa_I13521
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1532579
display id: component1532579
version: 1
top level: BBa_I13521
definition: BBa_B0010
access: public

BBa_R0040
type: Component
persistent identity: component1532558
display id: component1532558
version: 1
top level: BBa_I13521
definition: p(tetR)
access: public

BBa_E1010
type: Component
persistent identity: component1532573
display id: component1532573
version: 1
top level: BBa_I13521
definition: mRFP1
access: public

BBa_B0034
type: Component
persistent identity: component1532566
display id: component1532566
version: 1
top level: BBa_I13521
definition: BBa_B0034
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1532558
display id: annotation1532558
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532558/range1532558/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13521/annotation1532558/range1532558/1
type: Range
persistent identity: range1532558
display id: range1532558
version: 1
top level: BBa_I13521
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1532566
display id: annotation1532566
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532566/range1532566/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13521/annotation1532566/range1532566/1
type: Range
persistent identity: range1532566
display id: range1532566
version: 1
top level: BBa_I13521
direction: direction/forward
start: 63
end: 74
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1532573
display id: annotation1532573
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532573/range1532573/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13521/annotation1532573/range1532573/1
type: Range
persistent identity: range1532573
display id: range1532573
version: 1
top level: BBa_I13521
direction: direction/forward
start: 81
end: 761
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1532579
display id: annotation1532579
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532579/range1532579/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13521/annotation1532579/range1532579/1
type: Range
persistent identity: range1532579
display id: range1532579
version: 1
top level: BBa_I13521
direction: direction/forward
start: 795
end: 874
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1532589
display id: annotation1532589
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532589/range1532589/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13521/annotation1532589/range1532589/1
type: Range
persistent identity: range1532589
display id: range1532589
version: 1
top level: BBa_I13521
direction: direction/forward
start: 883
end: 923
orientation: inline

BBa_K1583101
type: ComponentDefinition
persistent identity: BBa K1583101
display id: BBa_K1583101
version: 1
was generated by: iGEM to SBOL conversion
description: pRha + CsgA + His-tag
created: 2015-09-03T11:00:00Z
modified: 2016-01-25T11:29:45Z
mutable provenance: The DNA was synthesized. The sequence of CsgA originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055). The design was based on the paper ""Programmable biofilm-based materials from engineered curli nanofibres""[1] 1. Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.
owned by: james
owned by: myers
mutable description: CsgA with HIS-tag attachted to the C-terminus under control of L-rhamnose-inducible promoter
discontinued: false
dominant: false
experience: experience/Works
group u list: _2000_
m user id: 4206
owner id: 24478
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The nucleotides encoding for the second and third amino acid of the CsgA gene were changed to optimize synthesis success. (silent mutations)
star: false
creator: Hector Sanguesa Ferrer, Max van
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K914003
component: BBa_G0002
component: BBa_K1583008
component: BBa_K1583050
component: BBa_K1583003
sequence annotation: BBa_G0002
sequence annotation: BBa_K914003
sequence annotation: BBa_K1583050
sequence annotation: BBa_K1583008
sequence annotation: BBa_K1583003
sequence: https://synbiohub.org/public/igem/BBa_K1583101_sequence/1

BBa_K914003
type: Component
persistent identity: component2443475
display id: component2443475
version: 1
top level: BBa_K1583101
definition: BBa_K914003
access: public

BBa_G0002
type: Component
persistent identity: component2443473
display id: component2443473
version: 1
top level: BBa_K1583101
definition: SX scar
access: public

BBa_K1583008
type: Component
persistent identity: component2443477
display id: component2443477
version: 1
top level: BBa_K1583101
definition: BBa_K1583008
access: public

BBa_K1583050
type: Component
persistent identity: component2443476
display id: component2443476
version: 1
top level: BBa_K1583101
definition: BBa_K1583050
access: public

BBa_K1583003
type: Component
persistent identity: component2443481
display id: component2443481
version: 1
top level: BBa_K1583101
definition: BBa_K1583003
access: public

BBa_G0002
type: SequenceAnnotation
persistent identity: annotation2443473
display id: annotation2443473
version: 1
top level: BBa_K1583101
location: https://synbiohub.org/public/igem/BBa_K1583101/annotation2443473/range2443473/1
component: BBa_G0002

https://synbiohub.org/public/igem/BBa_K1583101/annotation2443473/range2443473/1
type: Range
persistent identity: range2443473
display id: range2443473
version: 1
top level: BBa_K1583101
direction: direction/reverse
start: 1
end: 8
orientation: inline

BBa_K914003
type: SequenceAnnotation
persistent identity: annotation2443475
display id: annotation2443475
version: 1
top level: BBa_K1583101
location: https://synbiohub.org/public/igem/BBa_K1583101/annotation2443475/range2443475/1
component: BBa_K914003

https://synbiohub.org/public/igem/BBa_K1583101/annotation2443475/range2443475/1
type: Range
persistent identity: range2443475
display id: range2443475
version: 1
top level: BBa_K1583101
direction: direction/reverse
start: 9
end: 130
orientation: inline

BBa_K1583050
type: SequenceAnnotation
persistent identity: annotation2443476
display id: annotation2443476
version: 1
top level: BBa_K1583101
location: https://synbiohub.org/public/igem/BBa_K1583101/annotation2443476/range2443476/1
component: BBa_K1583050

https://synbiohub.org/public/igem/BBa_K1583101/annotation2443476/range2443476/1
type: Range
persistent identity: range2443476
display id: range2443476
version: 1
top level: BBa_K1583101
direction: direction/reverse
start: 131
end: 155
orientation: inline

BBa_K1583008
type: SequenceAnnotation
persistent identity: annotation2443477
display id: annotation2443477
version: 1
top level: BBa_K1583101
location: https://synbiohub.org/public/igem/BBa_K1583101/annotation2443477/range2443477/1
component: BBa_K1583008

https://synbiohub.org/public/igem/BBa_K1583101/annotation2443477/range2443477/1
type: Range
persistent identity: range2443477
display id: range2443477
version: 1
top level: BBa_K1583101
direction: direction/reverse
start: 156
end: 167
orientation: inline

BBa_K1583003
type: SequenceAnnotation
persistent identity: annotation2443481
display id: annotation2443481
version: 1
top level: BBa_K1583101
location: https://synbiohub.org/public/igem/BBa_K1583101/annotation2443481/range2443481/1
component: BBa_K1583003

https://synbiohub.org/public/igem/BBa_K1583101/annotation2443481/range2443481/1
type: Range
persistent identity: range2443481
display id: range2443481
version: 1
top level: BBa_K1583101
direction: direction/reverse
start: 169
end: 660
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

SX scar
type: ComponentDefinition
persistent identity: BBa G0002
display id: BBa_G0002
version: 1
was generated by: iGEM to SBOL conversion
description: SpeI/XbaI mixed site
created: 2007-02-26T12:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: XbaI and SpeI sites
owned by: james
owned by: myers
mutable description: XbaI/SpeI mixed site. Simply used to aid in entry of parts into the registry.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_G0002_sequence/1

BBa_K1583050
type: ComponentDefinition
persistent identity: BBa K1583050
display id: BBa_K1583050
version: 1
was generated by: iGEM to SBOL conversion
description: Scar 1
created: 2015-09-03T11:00:00Z
modified: 2015-09-18T07:33:29Z
mutable provenance: This scar is part of a synthesized construct.
owned by: james
owned by: myers
mutable description: Scar in constructs BBa_K1583100 - BBa_K1583112
discontinued: false
dominant: false
group u list: _2000_
m user id: 24478
owner id: 24478
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This scar originates from the part BBa_K1316015, on which the composite part BBa_K1583100 is based.
star: false
creator: Max van 't Hof
type: DnaRegion
role: SO:0001953
role: partType/Scar
sequence annotation: Scar
sequence: https://synbiohub.org/public/igem/BBa_K1583050_sequence/1

Scar
type: SequenceAnnotation
persistent identity: annotation2475064
display id: annotation2475064
version: 1
top level: BBa_K1583050
location: https://synbiohub.org/public/igem/BBa_K1583050/annotation2475064/range2475064/1
role: feature/scar

https://synbiohub.org/public/igem/BBa_K1583050/annotation2475064/range2475064/1
type: Range
persistent identity: range2475064
display id: range2475064
version: 1
top level: BBa_K1583050
direction: direction/forward
start: 1
end: 25
orientation: inline

BBa_K1583008
type: ComponentDefinition
persistent identity: BBa K1583008
display id: BBa_K1583008
version: 1
was generated by: iGEM to SBOL conversion
description: RBS
created: 2015-09-03T11:00:00Z
modified: 2015-09-04T05:18:43Z
mutable provenance: Synthesized
owned by: james
owned by: myers
mutable description: RBS
discontinued: false
dominant: false
experience: experience/None
group u list: _2000_
m user id: 24478
owner id: 24478
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Based on RBS of BBa_K1316015.
star: false
creator: Max van
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_K1583008_sequence/1

BBa_K914003
type: ComponentDefinition
persistent identity: BBa K914003
display id: BBa_K914003
version: 1
was generated by: iGEM to SBOL conversion
description: L-rhamnose-inducible promoter (pRha)
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:45Z
mutable provenance: Amplification of the plasmid pJOE3075 (Dr. Altenbuchner).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: L-rhamnose-inducible promoter is capable of high-level recombinant protein expression in the presence of L-rhamnose, it is also tightly regulated in the absence of L-rhamnose by the addition of D-glucose.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1179_
m user id: 0
owner id: 13487
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: One base pair modified (90: A -> T) to avoid EcoRI site. Mutation made in a less conserved base pair.
star: false
creator: Denis Samuylov
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: pRha
sequence: https://synbiohub.org/public/igem/BBa_K914003_sequence/1

pRha
type: SequenceAnnotation
persistent identity: annotation2188559
display id: annotation2188559
version: 1
top level: BBa_K914003
location: https://synbiohub.org/public/igem/BBa_K914003/annotation2188559/range2188559/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K914003/annotation2188559/range2188559/1
type: Range
persistent identity: range2188559
display id: range2188559
version: 1
top level: BBa_K914003
direction: direction/forward
start: 1
end: 122
orientation: inline

BBa_K1583003
type: ComponentDefinition
persistent identity: BBa K1583003
display id: BBa_K1583003
version: 1
was generated by: iGEM to SBOL conversion
description: CsgA_His
created: 2015-09-03T11:00:00Z
modified: 2015-09-04T04:16:37Z
mutable provenance: The DNA was synthesized. The sequence of CsgA originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055). The design was based on the paper "Programmable biofilm-based materials from engineered curli nanofibres"[1] 1. Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.
owned by: james
owned by: myers
mutable description: This gene encodes for the protein CsgA with a HIS-tag attached to it. CsgA is a curlin subunit that can self-assemble into curli after nucleation on CsgB. They are produced in an unpolymerized form and secreted from the cell. CsgA is naturally produced by e.g. E. coli K-12 MG1655 from the operon CsgBA. A second operon, CsgDEFG encodes four additional proteins that are required for the curli assembly and secretion.
discontinued: false
dominant: false
group u list: _2000_
m user id: 24478
owner id: 24478
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The nucleotides encoding for the third and fourth amino acids of the gene are changed to improve the success change of synthesis
star: false
creator: Max van 't Hof
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CsgA
sequence annotation: Linker
sequence annotation: HIS-tag
sequence: https://synbiohub.org/public/igem/BBa_K1583003_sequence/1

CsgA
type: SequenceAnnotation
persistent identity: annotation2443426
display id: annotation2443426
version: 1
top level: BBa_K1583003
location: https://synbiohub.org/public/igem/BBa_K1583003/annotation2443426/range2443426/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1583003/annotation2443426/range2443426/1
type: Range
persistent identity: range2443426
display id: range2443426
version: 1
top level: BBa_K1583003
direction: direction/forward
start: 1
end: 452
orientation: inline

Linker
type: SequenceAnnotation
persistent identity: annotation2443427
display id: annotation2443427
version: 1
top level: BBa_K1583003
location: https://synbiohub.org/public/igem/BBa_K1583003/annotation2443427/range2443427/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K1583003/annotation2443427/range2443427/1
type: Range
persistent identity: range2443427
display id: range2443427
version: 1
top level: BBa_K1583003
direction: direction/forward
start: 453
end: 471
orientation: inline

HIS-tag
type: SequenceAnnotation
persistent identity: annotation2443428
display id: annotation2443428
version: 1
top level: BBa_K1583003
location: https://synbiohub.org/public/igem/BBa_K1583003/annotation2443428/range2443428/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1583003/annotation2443428/range2443428/1
type: Range
persistent identity: range2443428
display id: range2443428
version: 1
top level: BBa_K1583003
direction: direction/forward
start: 472
end: 492
orientation: inline

https://synbiohub.org/public/igem/BBa_K1583107_sequence/1
type: Sequence
persistent identity: BBa K1583107 sequence
display id: BBa_K1583107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtactagagccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaatactagagcagcaaggaaatactagaaggaggtatataatgaaactgctgaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgcagcagttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtacggcagcggtggcagtggccatcaccaccatcaccactaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13521_sequence/1
type: Sequence
persistent identity: BBa I13521 sequence
display id: BBa_I13521_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583101_sequence/1
type: Sequence
persistent identity: BBa K1583101 sequence
display id: BBa_K1583101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagagccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaatactagagcagcaaggaaatactagaaggaggtatataatgaaactgctgaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgcagcagttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtacggcagcggtggcagtggccatcaccaccatcaccactaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G0002_sequence/1
type: Sequence
persistent identity: BBa G0002 sequence
display id: BBa_G0002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583050_sequence/1
type: Sequence
persistent identity: BBa K1583050 sequence
display id: BBa_K1583050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagagcagcaaggaaatactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583008_sequence/1
type: Sequence
persistent identity: BBa K1583008 sequence
display id: BBa_K1583008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaggaggtatat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K914003_sequence/1
type: Sequence
persistent identity: BBa K914003 sequence
display id: BBa_K914003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583003_sequence/1
type: Sequence
persistent identity: BBa K1583003 sequence
display id: BBa_K1583003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaactgctgaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgcagcagttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtacggcagcggtggcagtggccatcaccaccatcaccactaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z