##gff-version 3
##sequence-region BBa_K1583108 1 1615
BBa_K1583108	.	engineered_region	1	923	.	+	0	ID=BBa_I13521;Name=BBa_I13521
BBa_K1583108	.	terminator	795	874	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13521
BBa_K1583108	.	stem_loop	806	849	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1583108	.	engineered_region	795	874	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1583108	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13521
BBa_K1583108	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1583108	.	terminator	883	923	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13521
BBa_K1583108	.	polyA_site	910	923	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1583108	.	stem_loop	890	909	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1583108	.	engineered_region	883	923	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1583108	.	stop_codon	916	916	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1583108	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I13521
BBa_K1583108	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1583108	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1583108	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1583108	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1583108	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1583108	.	CDS	81	761	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13521
BBa_K1583108	.	engineered_tag	762	786	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1583108	.	CDS	81	755	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1583108	.	CDS	932	1615	.	+	0	ID=BBa_K1583102;Name=BBa_K1583102
BBa_K1583108	.	restriction_enzyme_assembly_scar	1054	1078	.	+	0	ID=BBa_K1583050;Name=BBa_K1583050;Parent=BBa_K1583102
BBa_K1583108	.	null	1054	1078	.	+	0	ID=annotation2475064;Name=Scar;Parent=BBa_K1583050
BBa_K1583108	.	ribosome_entry_site	1079	1090	.	+	0	ID=BBa_K1583008;Name=BBa_K1583008;Parent=BBa_K1583102
BBa_K1583108	.	restriction_enzyme_assembly_scar	1545	1562	.	+	0	ID=BBa_K1583015;Name=BBa_K1583015;Parent=BBa_K1583102
BBa_K1583108	.	non_covalent_binding_site	1545	1562	.	+	0	ID=annotation2474933;Name=Linker;Parent=BBa_K1583015
BBa_K1583108	.	promoter	932	1053	.	+	0	ID=BBa_K914003;Name=BBa_K914003;Parent=BBa_K1583102
BBa_K1583108	.	promoter	932	1053	.	+	0	ID=annotation2188559;Name=pRha;Parent=BBa_K914003
BBa_K1583108	.	CDS	1563	1601	.	+	0	ID=BBa_K1583060;Name=BBa_K1583060;Parent=BBa_K1583102
BBa_K1583108	.	non_covalent_binding_site	1563	1601	.	+	0	ID=annotation2473127;Name=Hydroxyapatite-affinity tag;Parent=BBa_K1583060
BBa_K1583108	.	CDS	1092	1544	.	+	0	ID=BBa_K1583014;Name=BBa_K1583014;Parent=BBa_K1583102
BBa_K1583108	.	CDS	1092	1544	.	+	0	ID=annotation2474839;Name=CsgA;Parent=BBa_K1583014
BBa_K1583108	.	restriction_enzyme_assembly_scar	1602	1615	.	+	0	ID=BBa_K1583051;Name=BBa_K1583051;Parent=BBa_K1583102
BBa_K1583108	.	null	1602	1615	.	+	0	ID=annotation2475065;Name=Scar;Parent=BBa_K1583051
>BBa_K1583108
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgag
ttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtc
cgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacat
cccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgtt
accgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccg
acggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaa
aggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaa
aaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcg
ttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagccacaattcagcaaatt
gtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcag
gcgctttttagactggtcgtaatgaatactagagcagcaaggaaatactagaaggaggtatataatgaaactgctgaaa
gtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaacc
acggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgc
tctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggc
tcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaatt
ctgaaatgacggttaaacagttcggtggtggcaacggtgctgcggttgaccagactgcatctaactcctccgtcaacgt
gactcaggttggctttggtaacaacgcgaccgctcatcagtacggcagcggtggcagtggctctgtttctgttggtatg
aaaccgtctccgcgtccgtaactactagaaggagg