BBa_K1583108
type: ComponentDefinition
persistent identity: BBa K1583108
display id: BBa_K1583108
version: 1
was generated by: iGEM to SBOL conversion
description: pRha + CsgA with Hydroxyapatite-affinity tag & pTet + RFP
created: 2015-09-07T11:00:00Z
modified: 2016-01-25T11:31:05Z
mutable provenance: The DNA was synthesized. The sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).
owned by: james
owned by: myers
mutable description: Upon induction with rhamnose, a fusion protein of CsgA with a hydroxyapatite affinity tag is expressed. The CsgA protein monomers can aggregate to form amyloid nanofibres. The affinity tag is supposed to increase adhesive properties of the biofilm created as a result of the CsgA induction. In order to better visualize the individual cells in the biofilm, we added the constitutive expression of RFP to our plasmid in a different operon. For our design, we took advantage of the double terminator present in the biobrick I13521 by placing it in front of our genes.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2000_
m user id: 4206
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: For our design, we took advantage of the double terminator present in the biobrick I13521 by placing it in front of our genes.
star: false
creator: Stefan Robert Marsden
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1583102
component: BBa_I13521
sequence annotation: BBa_I13521
sequence annotation: BBa_K1583102
sequence: https://synbiohub.org/public/igem/BBa_K1583108_sequence/1

BBa_K1583102
type: Component
persistent identity: component2461061
display id: component2461061
version: 1
top level: BBa_K1583108
definition: BBa_K1583102
access: public

BBa_I13521
type: Component
persistent identity: component2461052
display id: component2461052
version: 1
top level: BBa_K1583108
definition: BBa_I13521
access: public

BBa_I13521
type: SequenceAnnotation
persistent identity: annotation2461052
display id: annotation2461052
version: 1
top level: BBa_K1583108
location: https://synbiohub.org/public/igem/BBa_K1583108/annotation2461052/range2461052/1
component: BBa_I13521

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type: Range
persistent identity: range2461052
display id: range2461052
version: 1
top level: BBa_K1583108
direction: direction/reverse
start: 1
end: 923
orientation: inline

BBa_K1583102
type: SequenceAnnotation
persistent identity: annotation2461061
display id: annotation2461061
version: 1
top level: BBa_K1583108
location: https://synbiohub.org/public/igem/BBa_K1583108/annotation2461061/range2461061/1
component: BBa_K1583102

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type: Range
persistent identity: range2461061
display id: range2461061
version: 1
top level: BBa_K1583108
direction: direction/reverse
start: 932
end: 1615
orientation: inline

BBa_I13521
type: ComponentDefinition
persistent identity: BBa I13521
display id: BBa_I13521
version: 1
was generated by: iGEM to SBOL conversion
description: Ptet mRFP
created: 2005-06-21T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive on mRFP (positive control)
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_R0040
component: BBa_E1010
component: BBa_B0034
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13521_sequence/1

BBa_B0012
type: Component
persistent identity: component1532589
display id: component1532589
version: 1
top level: BBa_I13521
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1532579
display id: component1532579
version: 1
top level: BBa_I13521
definition: BBa_B0010
access: public

BBa_R0040
type: Component
persistent identity: component1532558
display id: component1532558
version: 1
top level: BBa_I13521
definition: p(tetR)
access: public

BBa_E1010
type: Component
persistent identity: component1532573
display id: component1532573
version: 1
top level: BBa_I13521
definition: mRFP1
access: public

BBa_B0034
type: Component
persistent identity: component1532566
display id: component1532566
version: 1
top level: BBa_I13521
definition: BBa_B0034
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1532558
display id: annotation1532558
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532558/range1532558/1
component: BBa_R0040

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type: Range
persistent identity: range1532558
display id: range1532558
version: 1
top level: BBa_I13521
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1532566
display id: annotation1532566
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532566/range1532566/1
component: BBa_B0034

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type: Range
persistent identity: range1532566
display id: range1532566
version: 1
top level: BBa_I13521
direction: direction/forward
start: 63
end: 74
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1532573
display id: annotation1532573
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532573/range1532573/1
component: BBa_E1010

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type: Range
persistent identity: range1532573
display id: range1532573
version: 1
top level: BBa_I13521
direction: direction/forward
start: 81
end: 761
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1532579
display id: annotation1532579
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532579/range1532579/1
component: BBa_B0010

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type: Range
persistent identity: range1532579
display id: range1532579
version: 1
top level: BBa_I13521
direction: direction/forward
start: 795
end: 874
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1532589
display id: annotation1532589
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532589/range1532589/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13521/annotation1532589/range1532589/1
type: Range
persistent identity: range1532589
display id: range1532589
version: 1
top level: BBa_I13521
direction: direction/forward
start: 883
end: 923
orientation: inline

BBa_K1583102
type: ComponentDefinition
persistent identity: BBa K1583102
display id: BBa_K1583102
version: 1
was generated by: iGEM to SBOL conversion
description: pRha + CsgA + Hydroxyapatite-affinity tag
created: 2015-09-07T11:00:00Z
modified: 2015-09-18T11:07:01Z
mutable provenance: The DNA was synthesized. The sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).
owned by: james
owned by: myers
mutable description: This part was synthesized. The rhamnose promoter was used by the iGEM 2014 TU Delft team and originally added by the iGEM12 Paris Bettencourt team. RBS and CsgA originate from E. coli K-12 MG1655. The nucleotides encoding for the second and third amino acid of the CsgA gene were changed to optimize synthesis success. (silent mutations). Upon induction with rhamnose, CsgA is expressed and exported into the extracellular space. There, the membrane protein CsgB can trigger the CsgA protein monomers to aggregate and form amyloid nanofibres.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2000_
m user id: 24478
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: The stop codon needed to be removed from CsgA to add the N-terminal hydroxyapatite affinity tag.
star: false
creator: Stefan Robert Marsden, Hector Sanguesa Ferrer
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_K1583014
component: BBa_K1583008
component: BBa_K1583050
component: BBa_K914003
component: BBa_K1583051
component: BBa_K1583060
component: BBa_K1583015
sequence annotation: BBa_K914003
sequence annotation: BBa_K1583050
sequence annotation: BBa_K1583008
sequence annotation: BBa_K1583014
sequence annotation: BBa_K1583015
sequence annotation: BBa_K1583060
sequence annotation: BBa_K1583051
sequence: https://synbiohub.org/public/igem/BBa_K1583102_sequence/1

BBa_K1583014
type: Component
persistent identity: component2461032
display id: component2461032
version: 1
top level: BBa_K1583102
definition: BBa_K1583014
access: public

BBa_K1583008
type: Component
persistent identity: component2461031
display id: component2461031
version: 1
top level: BBa_K1583102
definition: BBa_K1583008
access: public

BBa_K1583050
type: Component
persistent identity: component2461030
display id: component2461030
version: 1
top level: BBa_K1583102
definition: BBa_K1583050
access: public

BBa_K914003
type: Component
persistent identity: component2461029
display id: component2461029
version: 1
top level: BBa_K1583102
definition: BBa_K914003
access: public

BBa_K1583051
type: Component
persistent identity: component2461035
display id: component2461035
version: 1
top level: BBa_K1583102
definition: BBa_K1583051
access: public

BBa_K1583060
type: Component
persistent identity: component2461034
display id: component2461034
version: 1
top level: BBa_K1583102
definition: BBa_K1583060
access: public

BBa_K1583015
type: Component
persistent identity: component2461033
display id: component2461033
version: 1
top level: BBa_K1583102
definition: BBa_K1583015
access: public

BBa_K914003
type: SequenceAnnotation
persistent identity: annotation2461029
display id: annotation2461029
version: 1
top level: BBa_K1583102
location: https://synbiohub.org/public/igem/BBa_K1583102/annotation2461029/range2461029/1
component: BBa_K914003

https://synbiohub.org/public/igem/BBa_K1583102/annotation2461029/range2461029/1
type: Range
persistent identity: range2461029
display id: range2461029
version: 1
top level: BBa_K1583102
direction: direction/reverse
start: 1
end: 122
orientation: inline

BBa_K1583050
type: SequenceAnnotation
persistent identity: annotation2461030
display id: annotation2461030
version: 1
top level: BBa_K1583102
location: https://synbiohub.org/public/igem/BBa_K1583102/annotation2461030/range2461030/1
component: BBa_K1583050

https://synbiohub.org/public/igem/BBa_K1583102/annotation2461030/range2461030/1
type: Range
persistent identity: range2461030
display id: range2461030
version: 1
top level: BBa_K1583102
direction: direction/reverse
start: 123
end: 147
orientation: inline

BBa_K1583008
type: SequenceAnnotation
persistent identity: annotation2461031
display id: annotation2461031
version: 1
top level: BBa_K1583102
location: https://synbiohub.org/public/igem/BBa_K1583102/annotation2461031/range2461031/1
component: BBa_K1583008

https://synbiohub.org/public/igem/BBa_K1583102/annotation2461031/range2461031/1
type: Range
persistent identity: range2461031
display id: range2461031
version: 1
top level: BBa_K1583102
direction: direction/reverse
start: 148
end: 159
orientation: inline

BBa_K1583014
type: SequenceAnnotation
persistent identity: annotation2461032
display id: annotation2461032
version: 1
top level: BBa_K1583102
location: https://synbiohub.org/public/igem/BBa_K1583102/annotation2461032/range2461032/1
component: BBa_K1583014

https://synbiohub.org/public/igem/BBa_K1583102/annotation2461032/range2461032/1
type: Range
persistent identity: range2461032
display id: range2461032
version: 1
top level: BBa_K1583102
direction: direction/reverse
start: 161
end: 613
orientation: inline

BBa_K1583015
type: SequenceAnnotation
persistent identity: annotation2461033
display id: annotation2461033
version: 1
top level: BBa_K1583102
location: https://synbiohub.org/public/igem/BBa_K1583102/annotation2461033/range2461033/1
component: BBa_K1583015

https://synbiohub.org/public/igem/BBa_K1583102/annotation2461033/range2461033/1
type: Range
persistent identity: range2461033
display id: range2461033
version: 1
top level: BBa_K1583102
direction: direction/reverse
start: 614
end: 631
orientation: inline

BBa_K1583060
type: SequenceAnnotation
persistent identity: annotation2461034
display id: annotation2461034
version: 1
top level: BBa_K1583102
location: https://synbiohub.org/public/igem/BBa_K1583102/annotation2461034/range2461034/1
component: BBa_K1583060

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type: Range
persistent identity: range2461034
display id: range2461034
version: 1
top level: BBa_K1583102
direction: direction/reverse
start: 632
end: 670
orientation: inline

BBa_K1583051
type: SequenceAnnotation
persistent identity: annotation2461035
display id: annotation2461035
version: 1
top level: BBa_K1583102
location: https://synbiohub.org/public/igem/BBa_K1583102/annotation2461035/range2461035/1
component: BBa_K1583051

https://synbiohub.org/public/igem/BBa_K1583102/annotation2461035/range2461035/1
type: Range
persistent identity: range2461035
display id: range2461035
version: 1
top level: BBa_K1583102
direction: direction/reverse
start: 671
end: 684
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_K1583050
type: ComponentDefinition
persistent identity: BBa K1583050
display id: BBa_K1583050
version: 1
was generated by: iGEM to SBOL conversion
description: Scar 1
created: 2015-09-03T11:00:00Z
modified: 2015-09-18T07:33:29Z
mutable provenance: This scar is part of a synthesized construct.
owned by: james
owned by: myers
mutable description: Scar in constructs BBa_K1583100 - BBa_K1583112
discontinued: false
dominant: false
group u list: _2000_
m user id: 24478
owner id: 24478
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This scar originates from the part BBa_K1316015, on which the composite part BBa_K1583100 is based.
star: false
creator: Max van 't Hof
type: DnaRegion
role: SO:0001953
role: partType/Scar
sequence annotation: Scar
sequence: https://synbiohub.org/public/igem/BBa_K1583050_sequence/1

Scar
type: SequenceAnnotation
persistent identity: annotation2475064
display id: annotation2475064
version: 1
top level: BBa_K1583050
location: https://synbiohub.org/public/igem/BBa_K1583050/annotation2475064/range2475064/1
role: feature/scar

https://synbiohub.org/public/igem/BBa_K1583050/annotation2475064/range2475064/1
type: Range
persistent identity: range2475064
display id: range2475064
version: 1
top level: BBa_K1583050
direction: direction/forward
start: 1
end: 25
orientation: inline

BBa_K914003
type: ComponentDefinition
persistent identity: BBa K914003
display id: BBa_K914003
version: 1
was generated by: iGEM to SBOL conversion
description: L-rhamnose-inducible promoter (pRha)
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:45Z
mutable provenance: Amplification of the plasmid pJOE3075 (Dr. Altenbuchner).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: L-rhamnose-inducible promoter is capable of high-level recombinant protein expression in the presence of L-rhamnose, it is also tightly regulated in the absence of L-rhamnose by the addition of D-glucose.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1179_
m user id: 0
owner id: 13487
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: One base pair modified (90: A -> T) to avoid EcoRI site. Mutation made in a less conserved base pair.
star: false
creator: Denis Samuylov
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: pRha
sequence: https://synbiohub.org/public/igem/BBa_K914003_sequence/1

pRha
type: SequenceAnnotation
persistent identity: annotation2188559
display id: annotation2188559
version: 1
top level: BBa_K914003
location: https://synbiohub.org/public/igem/BBa_K914003/annotation2188559/range2188559/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K914003/annotation2188559/range2188559/1
type: Range
persistent identity: range2188559
display id: range2188559
version: 1
top level: BBa_K914003
direction: direction/forward
start: 1
end: 122
orientation: inline

BBa_K1583051
type: ComponentDefinition
persistent identity: BBa K1583051
display id: BBa_K1583051
version: 1
was generated by: iGEM to SBOL conversion
description: Scar 2
created: 2015-09-03T11:00:00Z
modified: 2015-09-18T07:34:25Z
mutable provenance: This scar is part of a synthesized construct.
owned by: james
owned by: myers
mutable description: Scar in constructs BBa_K1583100 - BBa_K1583112
discontinued: false
dominant: false
group u list: _2000_
m user id: 24478
owner id: 24478
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This scar originates from the part BBa_K1316015, on which the composite part BBa_K1583100 is based.
star: false
creator: Max van 't Hof
type: DnaRegion
role: SO:0001953
role: partType/Scar
sequence annotation: Scar
sequence: https://synbiohub.org/public/igem/BBa_K1583051_sequence/1

Scar
type: SequenceAnnotation
persistent identity: annotation2475065
display id: annotation2475065
version: 1
top level: BBa_K1583051
location: https://synbiohub.org/public/igem/BBa_K1583051/annotation2475065/range2475065/1
role: feature/scar

https://synbiohub.org/public/igem/BBa_K1583051/annotation2475065/range2475065/1
type: Range
persistent identity: range2475065
display id: range2475065
version: 1
top level: BBa_K1583051
direction: direction/forward
start: 1
end: 14
orientation: inline

BBa_K1583008
type: ComponentDefinition
persistent identity: BBa K1583008
display id: BBa_K1583008
version: 1
was generated by: iGEM to SBOL conversion
description: RBS
created: 2015-09-03T11:00:00Z
modified: 2015-09-04T05:18:43Z
mutable provenance: Synthesized
owned by: james
owned by: myers
mutable description: RBS
discontinued: false
dominant: false
experience: experience/None
group u list: _2000_
m user id: 24478
owner id: 24478
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Based on RBS of BBa_K1316015.
star: false
creator: Max van
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_K1583008_sequence/1

BBa_K1583014
type: ComponentDefinition
persistent identity: BBa K1583014
display id: BBa_K1583014
version: 1
was generated by: iGEM to SBOL conversion
description: CsgA missing the stop codon for creation of fusion proteins
created: 2015-09-03T11:00:00Z
modified: 2015-09-18T07:00:29Z
mutable provenance: The DNA was synthesized. The sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).
owned by: james
owned by: myers
mutable description: This part was designed to create a fusion protein of CsgA with Mfp3 (mussel foot protein). This protein has got high adhesive properties towards wet polar surfaces. Like this, an amyloid nanowire with high binding properties is created.
discontinued: false
dominant: false
group u list: _2000_
m user id: 24478
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The stop codon was deleted to create a fusion protein.
star: false
creator: Stefan Robert Marsden
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: CsgA
sequence: https://synbiohub.org/public/igem/BBa_K1583014_sequence/1

CsgA
type: SequenceAnnotation
persistent identity: annotation2474839
display id: annotation2474839
version: 1
top level: BBa_K1583014
location: https://synbiohub.org/public/igem/BBa_K1583014/annotation2474839/range2474839/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1583014/annotation2474839/range2474839/1
type: Range
persistent identity: range2474839
display id: range2474839
version: 1
top level: BBa_K1583014
direction: direction/forward
start: 1
end: 453
orientation: inline

BBa_K1583060
type: ComponentDefinition
persistent identity: BBa K1583060
display id: BBa_K1583060
version: 1
was generated by: iGEM to SBOL conversion
description: Hydroxyapatite affinity tag
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T04:31:43Z
mutable provenance: The sequence was obtained from the paper "Identification of a Highly Specific Hydroxyapatite-binding Peptide using Phage Display" [1] 1. M. Roy, S. Stanley, E. Amis, M. Becker, Adv. Mater., 2008, 20, 1830-1836.
owned by: james
owned by: myers
mutable description: This part generates a peptide tag which shows high adhesive properties towards hydroxyapatite, a main component of teeth.
discontinued: false
dominant: false
group u list: _2000_
m user id: 24463
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No specific design considerations were made.
star: false
creator: Stefan Robert Marsden
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Hydroxyapatite-affinity tag
sequence: https://synbiohub.org/public/igem/BBa_K1583060_sequence/1

Hydroxyapatite-affinity tag
type: SequenceAnnotation
persistent identity: annotation2473127
display id: annotation2473127
version: 1
top level: BBa_K1583060
location: https://synbiohub.org/public/igem/BBa_K1583060/annotation2473127/range2473127/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1583060/annotation2473127/range2473127/1
type: Range
persistent identity: range2473127
display id: range2473127
version: 1
top level: BBa_K1583060
direction: direction/forward
start: 1
end: 39
orientation: inline

BBa_K1583015
type: ComponentDefinition
persistent identity: BBa K1583015
display id: BBa_K1583015
version: 1
was generated by: iGEM to SBOL conversion
description: Linker in fusion protein
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T04:50:17Z
mutable provenance: The DNA was synthesized. The sequence of CsgA originates from the genomic DNA of E. coli K-12 MG1655.(http://www.ncbi.nlm.nih.gov/gene/949055). The design was based on the paper "Programmable biofilm-based materials from engineered curli nanofibres"[1] 1. Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.
owned by: james
owned by: myers
mutable description: A linker with the protein sequence GSGGSG was placed in between the CsgA and the affinity tag for increased performance reasons. The design was based on the paper "Programmable biofilm-based materials from engineered curli nanofibres"[1] 1. Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.
discontinued: false
dominant: false
group u list: _2000_
m user id: 24463
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We followed the design of "Programmable biofilm-based materials from engineered curli nanofibres"[1] 1. Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.
star: false
creator: Stefan Robert Marsden
type: DnaRegion
role: partType/Scar
role: SO:0001953
sequence annotation: Linker
sequence: https://synbiohub.org/public/igem/BBa_K1583015_sequence/1

Linker
type: SequenceAnnotation
persistent identity: annotation2474933
display id: annotation2474933
version: 1
top level: BBa_K1583015
location: https://synbiohub.org/public/igem/BBa_K1583015/annotation2474933/range2474933/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1583015/annotation2474933/range2474933/1
type: Range
persistent identity: range2474933
display id: range2474933
version: 1
top level: BBa_K1583015
direction: direction/forward
start: 1
end: 18
orientation: inline

https://synbiohub.org/public/igem/BBa_K1583108_sequence/1
type: Sequence
persistent identity: BBa K1583108 sequence
display id: BBa_K1583108_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaatactagagcagcaaggaaatactagaaggaggtatataatgaaactgctgaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgctgcggttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtacggcagcggtggcagtggctctgtttctgttggtatgaaaccgtctccgcgtccgtaactactagaaggagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13521_sequence/1
type: Sequence
persistent identity: BBa I13521 sequence
display id: BBa_I13521_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583102_sequence/1
type: Sequence
persistent identity: BBa K1583102 sequence
display id: BBa_K1583102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaatactagagcagcaaggaaatactagaaggaggtatataatgaaactgctgaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgctgcggttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtacggcagcggtggcagtggctctgtttctgttggtatgaaaccgtctccgcgtccgtaactactagaaggagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583050_sequence/1
type: Sequence
persistent identity: BBa K1583050 sequence
display id: BBa_K1583050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagagcagcaaggaaatactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K914003_sequence/1
type: Sequence
persistent identity: BBa K914003 sequence
display id: BBa_K914003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583051_sequence/1
type: Sequence
persistent identity: BBa K1583051 sequence
display id: BBa_K1583051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctactagaaggagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583008_sequence/1
type: Sequence
persistent identity: BBa K1583008 sequence
display id: BBa_K1583008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaggaggtatat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583014_sequence/1
type: Sequence
persistent identity: BBa K1583014 sequence
display id: BBa_K1583014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaactgctgaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgctgcggttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583060_sequence/1
type: Sequence
persistent identity: BBa K1583060 sequence
display id: BBa_K1583060_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctgtttctgttggtatgaaaccgtctccgcgtccgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583015_sequence/1
type: Sequence
persistent identity: BBa K1583015 sequence
display id: BBa_K1583015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcagcggtggcagtggc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z