LOCUS       BBa_K1583110            1767 bp    DNA     linear       27-Dec-2024
DEFINITION  pRha + Mfp5_CsgA_His fusion protein & pTET + RFP
ACCESSION   BBa_K1583110
VERSION     BBa_K1583110.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..755
                     /label=mrfp1
     misc_feature    762..786
                     /label=Help:Barcodes
     CDS             81..761
                     /label=BBa_E1010
     stem_loop       806..849
                     /label=stem_loop
     misc_feature    795..874
                     /label=BBa_B0010
     terminator      795..874
                     /label=BBa_B0010
     stem_loop       890..909
                     /label=T7 TE
     misc_feature    916..916
                     /label=stop
     polyA_site      910..923
                     /label=polya
     misc_feature    883..923
                     /label=BBa_B0012
     terminator      883..923
                     /label=BBa_B0012
     misc_feature    1..923
                     /label=BBa_I13521
     promoter        932..1053
                     /label=pRha
     promoter        932..1053
                     /label=BBa_K914003
     misc_feature    1054..1080
                     /label=Spacer
     misc_feature    1054..1080
                     /label=BBa_K1583056
     RBS             1081..1092
                     /label=RBS
     RBS             1081..1092
                     /label=BBa_K1583058
     CDS             1093..1323
                     /label=Mfp5
     CDS             1093..1323
                     /label=BBa_K1583002
     misc_feature    1324..1353
                     /label=Linker
     misc_feature    1324..1353
                     /label=BBa_K1583012
     CDS             1354..1746
                     /label=CsgA
     CDS             1354..1746
                     /label=BBa_K1583013
     misc_feature    1747..1767
                     /label=His-tag
     CDS             1747..1767
                     /label=BBa_K1583059
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt
      121 tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg
      181 aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc
      241 tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta
      301 aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg
      361 aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc
      421 aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc
      481 cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag
      541 acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg
      601 acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca
      661 aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt
      721 acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta
      781 gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      841 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      901 gggtgggcct ttctgcgttt atatactaga gccacaattc agcaaattgt gaacatcatc
      961 acgttcatct ttccctggtt gccaatggcc cattttcctg tcagtaacga gaaggtcgcg
     1021 tattcaggcg ctttttagac tggtcgtaat gaaatactag agcagcaagg aaatactaga
     1081 aggaggtata taatgagttc tgaagaatac aaaggtggtt attacccagg caatacttac
     1141 cactatcatt caggtggtag ttatcacgga tccggctatc atggaggata taagggaaag
     1201 tattacggaa aggcaaagaa atactattat aaatataaaa acagcggaaa atacaagtat
     1261 ctgaagaaag ctagaaaata ccatagaaag ggttacaaga agtattatgg aggtggtagc
     1321 agtggcggtg gcggtagcgg tggcggtggc agtggtgttg ttcctcagta cggcggcggc
     1381 ggtaaccacg gtggtggcgg taataatagc ggcccaaatt ctgagctgaa catttaccag
     1441 tacggtggcg gtaactctgc acttgctctg caaactgatg cccgtaactc tgacttgact
     1501 attacccagc atggcggcgg taatggtgca gatgttggtc agggctcaga tgacagctca
     1561 atcgatctga cccaacgtgg cttcggtaac agcgctactc ttgatcagtg gaacggcaaa
     1621 aattctgaaa tgacggttaa acagttcggt ggtggcaacg gtgctgcggt tgaccagact
     1681 gcatctaact cctccgtcaa cgtgactcag gttggctttg gtaacaacgc gaccgctcat
     1741 cagtaccatc accatcacca tcactaa
//