LOCUS       BBa_K1583111            1738 bp    DNA     linear       06-Dec-2025
DEFINITION  pRha + CsgA_Mfp3_His fusion protein & pTET + RFP
ACCESSION   BBa_K1583111
VERSION     BBa_K1583111.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..755
                     /label=mrfp1
     misc_feature    762..786
                     /label=Help:Barcodes
     CDS             81..761
                     /label=BBa_E1010
     stem_loop       806..849
                     /label=stem_loop
     misc_feature    795..874
                     /label=BBa_B0010
     terminator      795..874
                     /label=BBa_B0010
     stem_loop       890..909
                     /label=T7 TE
     misc_feature    916..916
                     /label=stop
     polyA_site      910..923
                     /label=polya
     misc_feature    883..923
                     /label=BBa_B0012
     terminator      883..923
                     /label=BBa_B0012
     misc_feature    1..923
                     /label=BBa_I13521
     promoter        930..1051
                     /label=pRha
     promoter        930..1051
                     /label=BBa_K914003
     misc_feature    1052..1078
                     /label=Scar
     misc_feature    1052..1078
                     /label=BBa_K1583063
     RBS             1079..1090
                     /label=RBS
     RBS             1079..1090
                     /label=BBa_K1583061
     misc_feature    1091..1096
                     /label=Scar
     misc_feature    1091..1096
                     /label=BBa_K1583062
     CDS             1097..1549
                     /label=CsgA
     CDS             1097..1549
                     /label=BBa_K1583014
     misc_feature    1550..1579
                     /label=Linker
     misc_feature    1550..1579
                     /label=BBa_K1583012
     CDS             1580..1717
                     /label=Mfp3
     CDS             1580..1717
                     /label=BBa_K1583011
     misc_feature    1718..1738
                     /label=His-tag
     CDS             1718..1738
                     /label=BBa_K1583059
     misc_feature    924..929
                     /label=Scar
     misc_feature    924..929
                     /label=BBa_K1583062
     promoter        932..1053
                     /label=pRha
     promoter        932..1053
                     /label=BBa_K914003
     misc_feature    1054..1080
                     /label=Scar
     misc_feature    1054..1080
                     /label=BBa_K1583063
     RBS             1081..1092
                     /label=RBS
     RBS             1081..1092
                     /label=BBa_K1583061
     misc_feature    1093..1098
                     /label=Scar
     misc_feature    1093..1098
                     /label=BBa_K1583062
     CDS             1099..1551
                     /label=CsgA
     CDS             1099..1551
                     /label=BBa_K1583014
     misc_feature    1552..1581
                     /label=Linker
     misc_feature    1552..1581
                     /label=BBa_K1583012
     CDS             1582..1719
                     /label=Mfp3
     CDS             1582..1719
                     /label=BBa_K1583011
     misc_feature    1720..1740
                     /label=His-tag
     CDS             1720..1740
                     /label=BBa_K1583059
     CDS             924..1738
                     /label=BBa_K1583105
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt
      121 tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg
      181 aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc
      241 tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta
      301 aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg
      361 aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc
      421 aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc
      481 cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag
      541 acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg
      601 acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca
      661 aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt
      721 acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta
      781 gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      841 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      901 gggtgggcct ttctgcgttt atatagtagc cacaattcag caaattgtga acatcatcac
      961 gttcatcttt ccctggttgc caatggccca ttttcctgtc agtaacgaga aggtcgcgta
     1021 ttcaggcgct ttttagactg gtcgtaatga aatactagag cagcaggtac ctactagaaa
     1081 agaggagaaa tagtagatga aactgctgaa agtagcagca attgcagcaa tcgtattctc
     1141 cggtagcgct ctggcaggtg ttgttcctca gtacggcggc ggcggtaacc acggtggtgg
     1201 cggtaataat agcggcccaa attctgagct gaacatttac cagtacggtg gcggtaactc
     1261 tgcacttgct ctgcaaactg atgcccgtaa ctctgacttg actattaccc agcatggcgg
     1321 cggtaatggt gcagatgttg gtcagggctc agatgacagc tcaatcgatc tgacccaacg
     1381 tggcttcggt aacagcgcta ctcttgatca gtggaacggc aaaaattctg aaatgacggt
     1441 taaacagttc ggtggtggca acggtgctgc ggttgaccag actgcatcta actcctccgt
     1501 caacgtgact caggttggct ttggtaacaa cgcgaccgct catcagtacg gcggtggcgg
     1561 tagcggtggc ggtggcagtg ctgattatta tggtccaaag tatggtcctc caagacgcta
     1621 cggtggtggc aactacaata gatatggcag acgttatggc gggtataaag gctggaacaa
     1681 tggttggaaa agaggtcgat ggggacgaaa gtattatcat caccatcacc atcactaa
//