##gff-version 3
##sequence-region BBa_K1583111 1 1738
BBa_K1583111	.	CDS	1097	1549	.	+	0	ID=BBa_K1583014;Name=BBa_K1583014
BBa_K1583111	.	CDS	1097	1549	.	+	0	ID=annotation2474839;Name=CsgA;Parent=BBa_K1583014
BBa_K1583111	.	ribosome_entry_site	1079	1090	.	+	0	ID=BBa_K1583061;Name=BBa_K1583061
BBa_K1583111	.	ribosome_entry_site	1079	1090	.	+	0	ID=annotation2475161;Name=RBS;Parent=BBa_K1583061
BBa_K1583111	.	restriction_enzyme_assembly_scar	1052	1078	.	+	0	ID=BBa_K1583063;Name=BBa_K1583063
BBa_K1583111	.	null	1052	1078	.	+	0	ID=annotation2475163;Name=Scar;Parent=BBa_K1583063
BBa_K1583111	.	promoter	930	1051	.	+	0	ID=BBa_K914003;Name=BBa_K914003
BBa_K1583111	.	promoter	930	1051	.	+	0	ID=annotation2188559;Name=pRha;Parent=BBa_K914003
BBa_K1583111	.	engineered_region	1	923	.	+	0	ID=BBa_I13521;Name=BBa_I13521
BBa_K1583111	.	terminator	795	874	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13521
BBa_K1583111	.	stem_loop	806	849	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1583111	.	engineered_region	795	874	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1583111	.	CDS	81	761	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13521
BBa_K1583111	.	CDS	81	755	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1583111	.	engineered_tag	762	786	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1583111	.	terminator	883	923	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13521
BBa_K1583111	.	stem_loop	890	909	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1583111	.	polyA_site	910	923	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1583111	.	stop_codon	916	916	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1583111	.	engineered_region	883	923	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1583111	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I13521
BBa_K1583111	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1583111	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1583111	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1583111	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1583111	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1583111	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13521
BBa_K1583111	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1583111	.	restriction_enzyme_assembly_scar	1091	1096	.	+	0	ID=BBa_K1583062;Name=BBa_K1583062
BBa_K1583111	.	null	1091	1096	.	+	0	ID=annotation2475162;Name=Scar;Parent=BBa_K1583062
BBa_K1583111	.	CDS	1718	1738	.	+	0	ID=BBa_K1583059;Name=BBa_K1583059
BBa_K1583111	.	non_covalent_binding_site	1718	1738	.	+	0	ID=annotation2473195;Name=His-tag;Parent=BBa_K1583059
BBa_K1583111	.	CDS	924	1738	.	+	0	ID=BBa_K1583105;Name=BBa_K1583105
BBa_K1583111	.	restriction_enzyme_assembly_scar	1093	1098	.	+	0	ID=BBa_K1583062;Name=BBa_K1583062;Parent=BBa_K1583105
BBa_K1583111	.	null	1093	1098	.	+	0	ID=annotation2475162;Name=Scar;Parent=BBa_K1583062
BBa_K1583111	.	promoter	932	1053	.	+	0	ID=BBa_K914003;Name=BBa_K914003;Parent=BBa_K1583105
BBa_K1583111	.	promoter	932	1053	.	+	0	ID=annotation2188559;Name=pRha;Parent=BBa_K914003
BBa_K1583111	.	restriction_enzyme_assembly_scar	1054	1080	.	+	0	ID=BBa_K1583063;Name=BBa_K1583063;Parent=BBa_K1583105
BBa_K1583111	.	null	1054	1080	.	+	0	ID=annotation2475163;Name=Scar;Parent=BBa_K1583063
BBa_K1583111	.	restriction_enzyme_assembly_scar	1552	1581	.	+	0	ID=BBa_K1583012;Name=BBa_K1583012;Parent=BBa_K1583105
BBa_K1583111	.	non_covalent_binding_site	1552	1581	.	+	0	ID=annotation2474770;Name=Linker;Parent=BBa_K1583012
BBa_K1583111	.	CDS	1582	1719	.	+	0	ID=BBa_K1583011;Name=BBa_K1583011;Parent=BBa_K1583105
BBa_K1583111	.	CDS	1582	1719	.	+	0	ID=annotation2474749;Name=Mfp3;Parent=BBa_K1583011
BBa_K1583111	.	restriction_enzyme_assembly_scar	924	929	.	+	0	ID=BBa_K1583062;Name=BBa_K1583062;Parent=BBa_K1583105
BBa_K1583111	.	null	924	929	.	+	0	ID=annotation2475162;Name=Scar;Parent=BBa_K1583062
BBa_K1583111	.	ribosome_entry_site	1081	1092	.	+	0	ID=BBa_K1583061;Name=BBa_K1583061;Parent=BBa_K1583105
BBa_K1583111	.	ribosome_entry_site	1081	1092	.	+	0	ID=annotation2475161;Name=RBS;Parent=BBa_K1583061
BBa_K1583111	.	CDS	1099	1551	.	+	0	ID=BBa_K1583014;Name=BBa_K1583014;Parent=BBa_K1583105
BBa_K1583111	.	CDS	1099	1551	.	+	0	ID=annotation2474839;Name=CsgA;Parent=BBa_K1583014
BBa_K1583111	.	CDS	1720	1740	.	+	0	ID=BBa_K1583059;Name=BBa_K1583059;Parent=BBa_K1583105
BBa_K1583111	.	non_covalent_binding_site	1720	1740	.	+	0	ID=annotation2473195;Name=His-tag;Parent=BBa_K1583059
BBa_K1583111	.	restriction_enzyme_assembly_scar	1550	1579	.	+	0	ID=BBa_K1583012;Name=BBa_K1583012
BBa_K1583111	.	non_covalent_binding_site	1550	1579	.	+	0	ID=annotation2474770;Name=Linker;Parent=BBa_K1583012
BBa_K1583111	.	CDS	1580	1717	.	+	0	ID=BBa_K1583011;Name=BBa_K1583011
BBa_K1583111	.	CDS	1580	1717	.	+	0	ID=annotation2474749;Name=Mfp3;Parent=BBa_K1583011
>BBa_K1583111
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgag
ttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtc
cgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacat
cccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgtt
accgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccg
acggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaa
aggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaa
aaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcg
ttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatagtagccacaattcagcaaattgt
gaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggc
gctttttagactggtcgtaatgaaatactagagcagcaggtacctactagaaaagaggagaaatagtagatgaaactgc
tgaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcgg
taaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgca
cttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtc
agggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaa
aaattctgaaatgacggttaaacagttcggtggtggcaacggtgctgcggttgaccagactgcatctaactcctccgtc
aacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtacggcggtggcggtagcggtggcggtggcagtg
ctgattattatggtccaaagtatggtcctccaagacgctacggtggtggcaactacaatagatatggcagacgttatgg
cgggtataaaggctggaacaatggttggaaaagaggtcgatggggacgaaagtattatcatcaccatcaccatcactaa