BBa_K1583111
type: ComponentDefinition
persistent identity: BBa K1583111
display id: BBa_K1583111
version: 1
was generated by: iGEM to SBOL conversion
description: pRha + CsgA_Mfp3_His fusion protein & pTET + RFP
created: 2015-09-03T11:00:00Z
modified: 2015-09-18T03:38:33Z
mutable provenance: The sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).
owned by: james
owned by: myers
mutable description: The fusion protein of CsgA with Mfp3 and N-terminal His tag was improved by insertion of constitutively expressed RFP in a different operon to make the cells red fluorescent.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2000_
m user id: 24478
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: I13521 was placed in front of K1583007 to take advantage of the double terminator in I13521.
star: false
creator: Stefan Robert Marsden
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_I13521
component: BBa_K1583063
component: BBa_K914003
component: BBa_K1583062
component: BBa_K1583061
component: BBa_K1583012
component: BBa_K1583014
component: BBa_K1583059
component: BBa_K1583011
component: BBa_K1583105
sequence annotation: BBa_I13521
sequence annotation: BBa_K1583105
sequence annotation: BBa_K914003
sequence annotation: BBa_K1583063
sequence annotation: BBa_K1583061
sequence annotation: BBa_K1583062
sequence annotation: BBa_K1583014
sequence annotation: BBa_K1583012
sequence annotation: BBa_K1583011
sequence annotation: BBa_K1583059
sequence: https://synbiohub.org/public/igem/BBa_K1583111_sequence/1

BBa_I13521
type: Component
persistent identity: component2453869
display id: component2453869
version: 1
top level: BBa_K1583111
definition: BBa_I13521
access: public

BBa_K1583063
type: Component
persistent identity: component2453873
display id: component2453873
version: 1
top level: BBa_K1583111
definition: BBa_K1583063
access: public

BBa_K914003
type: Component
persistent identity: component2453871
display id: component2453871
version: 1
top level: BBa_K1583111
definition: BBa_K914003
access: public

BBa_K1583062
type: Component
persistent identity: component2453875
display id: component2453875
version: 1
top level: BBa_K1583111
definition: BBa_K1583062
access: public

BBa_K1583061
type: Component
persistent identity: component2453874
display id: component2453874
version: 1
top level: BBa_K1583111
definition: BBa_K1583061
access: public

BBa_K1583012
type: Component
persistent identity: component2453877
display id: component2453877
version: 1
top level: BBa_K1583111
definition: BBa_K1583012
access: public

BBa_K1583014
type: Component
persistent identity: component2453876
display id: component2453876
version: 1
top level: BBa_K1583111
definition: BBa_K1583014
access: public

BBa_K1583059
type: Component
persistent identity: component2453879
display id: component2453879
version: 1
top level: BBa_K1583111
definition: BBa_K1583059
access: public

BBa_K1583011
type: Component
persistent identity: component2453878
display id: component2453878
version: 1
top level: BBa_K1583111
definition: BBa_K1583011
access: public

BBa_K1583105
type: Component
persistent identity: component2453880
display id: component2453880
version: 1
top level: BBa_K1583111
definition: BBa_K1583105
access: public

BBa_I13521
type: SequenceAnnotation
persistent identity: annotation2453869
display id: annotation2453869
version: 1
top level: BBa_K1583111
location: https://synbiohub.org/public/igem/BBa_K1583111/annotation2453869/range2453869/1
component: BBa_I13521

https://synbiohub.org/public/igem/BBa_K1583111/annotation2453869/range2453869/1
type: Range
persistent identity: range2453869
display id: range2453869
version: 1
top level: BBa_K1583111
direction: direction/reverse
start: 1
end: 923
orientation: inline

BBa_K1583105
type: SequenceAnnotation
persistent identity: annotation2453880
display id: annotation2453880
version: 1
top level: BBa_K1583111
location: https://synbiohub.org/public/igem/BBa_K1583111/annotation2453880/range2453880/1
component: BBa_K1583105

https://synbiohub.org/public/igem/BBa_K1583111/annotation2453880/range2453880/1
type: Range
persistent identity: range2453880
display id: range2453880
version: 1
top level: BBa_K1583111
direction: direction/reverse
start: 924
end: 1738
orientation: inline

BBa_K914003
type: SequenceAnnotation
persistent identity: annotation2453871
display id: annotation2453871
version: 1
top level: BBa_K1583111
location: https://synbiohub.org/public/igem/BBa_K1583111/annotation2453871/range2453871/1
component: BBa_K914003

https://synbiohub.org/public/igem/BBa_K1583111/annotation2453871/range2453871/1
type: Range
persistent identity: range2453871
display id: range2453871
version: 1
top level: BBa_K1583111
direction: direction/reverse
start: 930
end: 1051
orientation: inline

BBa_K1583063
type: SequenceAnnotation
persistent identity: annotation2453873
display id: annotation2453873
version: 1
top level: BBa_K1583111
location: https://synbiohub.org/public/igem/BBa_K1583111/annotation2453873/range2453873/1
component: BBa_K1583063

https://synbiohub.org/public/igem/BBa_K1583111/annotation2453873/range2453873/1
type: Range
persistent identity: range2453873
display id: range2453873
version: 1
top level: BBa_K1583111
direction: direction/reverse
start: 1052
end: 1078
orientation: inline

BBa_K1583061
type: SequenceAnnotation
persistent identity: annotation2453874
display id: annotation2453874
version: 1
top level: BBa_K1583111
location: https://synbiohub.org/public/igem/BBa_K1583111/annotation2453874/range2453874/1
component: BBa_K1583061

https://synbiohub.org/public/igem/BBa_K1583111/annotation2453874/range2453874/1
type: Range
persistent identity: range2453874
display id: range2453874
version: 1
top level: BBa_K1583111
direction: direction/reverse
start: 1079
end: 1090
orientation: inline

BBa_K1583062
type: SequenceAnnotation
persistent identity: annotation2453875
display id: annotation2453875
version: 1
top level: BBa_K1583111
location: https://synbiohub.org/public/igem/BBa_K1583111/annotation2453875/range2453875/1
component: BBa_K1583062

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type: Range
persistent identity: range2453875
display id: range2453875
version: 1
top level: BBa_K1583111
direction: direction/reverse
start: 1091
end: 1096
orientation: inline

BBa_K1583014
type: SequenceAnnotation
persistent identity: annotation2453876
display id: annotation2453876
version: 1
top level: BBa_K1583111
location: https://synbiohub.org/public/igem/BBa_K1583111/annotation2453876/range2453876/1
component: BBa_K1583014

https://synbiohub.org/public/igem/BBa_K1583111/annotation2453876/range2453876/1
type: Range
persistent identity: range2453876
display id: range2453876
version: 1
top level: BBa_K1583111
direction: direction/reverse
start: 1097
end: 1549
orientation: inline

BBa_K1583012
type: SequenceAnnotation
persistent identity: annotation2453877
display id: annotation2453877
version: 1
top level: BBa_K1583111
location: https://synbiohub.org/public/igem/BBa_K1583111/annotation2453877/range2453877/1
component: BBa_K1583012

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type: Range
persistent identity: range2453877
display id: range2453877
version: 1
top level: BBa_K1583111
direction: direction/reverse
start: 1550
end: 1579
orientation: inline

BBa_K1583011
type: SequenceAnnotation
persistent identity: annotation2453878
display id: annotation2453878
version: 1
top level: BBa_K1583111
location: https://synbiohub.org/public/igem/BBa_K1583111/annotation2453878/range2453878/1
component: BBa_K1583011

https://synbiohub.org/public/igem/BBa_K1583111/annotation2453878/range2453878/1
type: Range
persistent identity: range2453878
display id: range2453878
version: 1
top level: BBa_K1583111
direction: direction/reverse
start: 1580
end: 1717
orientation: inline

BBa_K1583059
type: SequenceAnnotation
persistent identity: annotation2453879
display id: annotation2453879
version: 1
top level: BBa_K1583111
location: https://synbiohub.org/public/igem/BBa_K1583111/annotation2453879/range2453879/1
component: BBa_K1583059

https://synbiohub.org/public/igem/BBa_K1583111/annotation2453879/range2453879/1
type: Range
persistent identity: range2453879
display id: range2453879
version: 1
top level: BBa_K1583111
direction: direction/reverse
start: 1718
end: 1738
orientation: inline

BBa_I13521
type: ComponentDefinition
persistent identity: BBa I13521
display id: BBa_I13521
version: 1
was generated by: iGEM to SBOL conversion
description: Ptet mRFP
created: 2005-06-21T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive on mRFP (positive control)
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_R0040
component: BBa_E1010
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13521_sequence/1

BBa_R0040
type: Component
persistent identity: component1532558
display id: component1532558
version: 1
top level: BBa_I13521
definition: p(tetR)
access: public

BBa_E1010
type: Component
persistent identity: component1532573
display id: component1532573
version: 1
top level: BBa_I13521
definition: mRFP1
access: public

BBa_B0034
type: Component
persistent identity: component1532566
display id: component1532566
version: 1
top level: BBa_I13521
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1532589
display id: component1532589
version: 1
top level: BBa_I13521
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1532579
display id: component1532579
version: 1
top level: BBa_I13521
definition: BBa_B0010
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1532558
display id: annotation1532558
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532558/range1532558/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13521/annotation1532558/range1532558/1
type: Range
persistent identity: range1532558
display id: range1532558
version: 1
top level: BBa_I13521
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1532566
display id: annotation1532566
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532566/range1532566/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13521/annotation1532566/range1532566/1
type: Range
persistent identity: range1532566
display id: range1532566
version: 1
top level: BBa_I13521
direction: direction/forward
start: 63
end: 74
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1532573
display id: annotation1532573
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532573/range1532573/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13521/annotation1532573/range1532573/1
type: Range
persistent identity: range1532573
display id: range1532573
version: 1
top level: BBa_I13521
direction: direction/forward
start: 81
end: 761
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1532579
display id: annotation1532579
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532579/range1532579/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13521/annotation1532579/range1532579/1
type: Range
persistent identity: range1532579
display id: range1532579
version: 1
top level: BBa_I13521
direction: direction/forward
start: 795
end: 874
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1532589
display id: annotation1532589
version: 1
top level: BBa_I13521
location: https://synbiohub.org/public/igem/BBa_I13521/annotation1532589/range1532589/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13521/annotation1532589/range1532589/1
type: Range
persistent identity: range1532589
display id: range1532589
version: 1
top level: BBa_I13521
direction: direction/forward
start: 883
end: 923
orientation: inline

BBa_K1583011
type: ComponentDefinition
persistent identity: BBa K1583011
display id: BBa_K1583011
version: 1
was generated by: iGEM to SBOL conversion
description: Mfp3 adhesive protein
created: 2015-09-03T11:00:00Z
modified: 2015-09-18T06:40:59Z
mutable provenance: This DNA was synthesized. The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1] 1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.
owned by: james
owned by: myers
mutable description: Inspired by mussels, the Mfp3 (mussel foot protein) has high adhesive properties towards wet polar surfaces.
discontinued: false
dominant: false
group u list: _2000_
m user id: 24478
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No special design considerations were taken.
star: false
creator: Stefan Robert Marsden
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Mfp3
sequence: https://synbiohub.org/public/igem/BBa_K1583011_sequence/1

Mfp3
type: SequenceAnnotation
persistent identity: annotation2474749
display id: annotation2474749
version: 1
top level: BBa_K1583011
location: https://synbiohub.org/public/igem/BBa_K1583011/annotation2474749/range2474749/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1583011/annotation2474749/range2474749/1
type: Range
persistent identity: range2474749
display id: range2474749
version: 1
top level: BBa_K1583011
direction: direction/forward
start: 1
end: 138
orientation: inline

BBa_K1583012
type: ComponentDefinition
persistent identity: BBa K1583012
display id: BBa_K1583012
version: 1
was generated by: iGEM to SBOL conversion
description: Linker in fusion protein
created: 2015-09-03T11:00:00Z
modified: 2015-09-18T06:53:59Z
mutable provenance: This DNA was synthesized. The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1] 1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.
owned by: james
owned by: myers
mutable description: This amino acid sequence was used as a linker to create a fusion protein from Mfp3/Mfp5 with CsgA.
discontinued: false
dominant: false
group u list: _2000_
m user id: 24478
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No special design considerations needed to be taken.
star: false
creator: Stefan Robert Marsden
type: DnaRegion
role: SO:0001953
role: partType/Scar
sequence annotation: Linker
sequence: https://synbiohub.org/public/igem/BBa_K1583012_sequence/1

Linker
type: SequenceAnnotation
persistent identity: annotation2474770
display id: annotation2474770
version: 1
top level: BBa_K1583012
location: https://synbiohub.org/public/igem/BBa_K1583012/annotation2474770/range2474770/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K1583012/annotation2474770/range2474770/1
type: Range
persistent identity: range2474770
display id: range2474770
version: 1
top level: BBa_K1583012
direction: direction/forward
start: 1
end: 30
orientation: inline

BBa_K1583059
type: ComponentDefinition
persistent identity: BBa K1583059
display id: BBa_K1583059
version: 1
was generated by: iGEM to SBOL conversion
description: His-tag without start codon
created: 2015-09-03T11:00:00Z
modified: 2015-09-18T11:05:34Z
mutable provenance: Standard DNA sequence optimized for E.coli.
owned by: james
owned by: myers
mutable description: Addition of a His tag at the N-terminal end of a protein of interest is goal of this basic part.
discontinued: false
dominant: false
experience: experience/None
group u list: _2000_
m user id: 24478
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The codon was optimized for synthesis to avoid repetetive motives of too many codons.
star: false
creator: Stefan Robert Marsden
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: His-tag
sequence: https://synbiohub.org/public/igem/BBa_K1583059_sequence/1

His-tag
type: SequenceAnnotation
persistent identity: annotation2473195
display id: annotation2473195
version: 1
top level: BBa_K1583059
location: https://synbiohub.org/public/igem/BBa_K1583059/annotation2473195/range2473195/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1583059/annotation2473195/range2473195/1
type: Range
persistent identity: range2473195
display id: range2473195
version: 1
top level: BBa_K1583059
direction: direction/forward
start: 1
end: 21
orientation: inline

BBa_K1583061
type: ComponentDefinition
persistent identity: BBa K1583061
display id: BBa_K1583061
version: 1
was generated by: iGEM to SBOL conversion
description: RBS
created: 2015-09-07T11:00:00Z
modified: 2015-09-08T07:43:48Z
mutable provenance: The DNA was synthesized. The sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055). The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1] 1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.
owned by: james
owned by: myers
mutable description: This RBS was used in our constructs Mfp5+CsgA and CsgA+Mfp3. CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms taken from E.coli K-12 MG1655. Inspired by mussels, the Mfp5 (mussel foot protein) has high adhesive properties towards wet polar surfaces. This DNA was synthesized. The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1] 1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.
discontinued: false
dominant: false
group u list: _2000_
m user id: 24463
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1] 1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.
star: false
creator: Stefan Robert Marsden
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_K1583061_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2475161
display id: annotation2475161
version: 1
top level: BBa_K1583061
location: https://synbiohub.org/public/igem/BBa_K1583061/annotation2475161/range2475161/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1583061/annotation2475161/range2475161/1
type: Range
persistent identity: range2475161
display id: range2475161
version: 1
top level: BBa_K1583061
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_K1583014
type: ComponentDefinition
persistent identity: BBa K1583014
display id: BBa_K1583014
version: 1
was generated by: iGEM to SBOL conversion
description: CsgA missing the stop codon for creation of fusion proteins
created: 2015-09-03T11:00:00Z
modified: 2015-09-18T07:00:29Z
mutable provenance: The DNA was synthesized. The sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).
owned by: james
owned by: myers
mutable description: This part was designed to create a fusion protein of CsgA with Mfp3 (mussel foot protein). This protein has got high adhesive properties towards wet polar surfaces. Like this, an amyloid nanowire with high binding properties is created.
discontinued: false
dominant: false
group u list: _2000_
m user id: 24478
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The stop codon was deleted to create a fusion protein.
star: false
creator: Stefan Robert Marsden
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: CsgA
sequence: https://synbiohub.org/public/igem/BBa_K1583014_sequence/1

CsgA
type: SequenceAnnotation
persistent identity: annotation2474839
display id: annotation2474839
version: 1
top level: BBa_K1583014
location: https://synbiohub.org/public/igem/BBa_K1583014/annotation2474839/range2474839/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1583014/annotation2474839/range2474839/1
type: Range
persistent identity: range2474839
display id: range2474839
version: 1
top level: BBa_K1583014
direction: direction/forward
start: 1
end: 453
orientation: inline

BBa_K1583062
type: ComponentDefinition
persistent identity: BBa K1583062
display id: BBa_K1583062
version: 1
was generated by: iGEM to SBOL conversion
description: Scar in CsgA_Mfp3
created: 2015-09-07T11:00:00Z
modified: 2015-09-18T07:52:53Z
mutable provenance: The DNA was synthesized. The sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).
owned by: james
owned by: myers
mutable description: This scar is part of a synthesized construct. It is used in BBa_K1583007.
discontinued: false
dominant: false
experience: experience/None
group u list: _2000_
m user id: 24478
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No special desing considerations.
star: false
creator: Stefan Robert Marsden
type: DnaRegion
role: SO:0001953
role: partType/Scar
sequence annotation: Scar
sequence: https://synbiohub.org/public/igem/BBa_K1583062_sequence/1

Scar
type: SequenceAnnotation
persistent identity: annotation2475162
display id: annotation2475162
version: 1
top level: BBa_K1583062
location: https://synbiohub.org/public/igem/BBa_K1583062/annotation2475162/range2475162/1
role: feature/scar

https://synbiohub.org/public/igem/BBa_K1583062/annotation2475162/range2475162/1
type: Range
persistent identity: range2475162
display id: range2475162
version: 1
top level: BBa_K1583062
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_K1583063
type: ComponentDefinition
persistent identity: BBa K1583063
display id: BBa_K1583063
version: 1
was generated by: iGEM to SBOL conversion
description: Scar in CsgA_Mfp3
created: 2015-09-07T11:00:00Z
modified: 2015-09-18T08:02:08Z
mutable provenance: The DNA was synthesized. The sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).
owned by: james
owned by: myers
mutable description: This scar is part of a synthesized construct. It is used in BBa_K1583007.
discontinued: false
dominant: false
experience: experience/None
group u list: _2000_
m user id: 24478
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No special design considerations.
star: false
creator: Stefan Robert Marsden
type: DnaRegion
role: SO:0001953
role: partType/Scar
sequence annotation: Scar
sequence: https://synbiohub.org/public/igem/BBa_K1583063_sequence/1

Scar
type: SequenceAnnotation
persistent identity: annotation2475163
display id: annotation2475163
version: 1
top level: BBa_K1583063
location: https://synbiohub.org/public/igem/BBa_K1583063/annotation2475163/range2475163/1
role: feature/scar

https://synbiohub.org/public/igem/BBa_K1583063/annotation2475163/range2475163/1
type: Range
persistent identity: range2475163
display id: range2475163
version: 1
top level: BBa_K1583063
direction: direction/forward
start: 1
end: 27
orientation: inline

BBa_K914003
type: ComponentDefinition
persistent identity: BBa K914003
display id: BBa_K914003
version: 1
was generated by: iGEM to SBOL conversion
description: L-rhamnose-inducible promoter (pRha)
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:45Z
mutable provenance: Amplification of the plasmid pJOE3075 (Dr. Altenbuchner).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: L-rhamnose-inducible promoter is capable of high-level recombinant protein expression in the presence of L-rhamnose, it is also tightly regulated in the absence of L-rhamnose by the addition of D-glucose.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1179_
m user id: 0
owner id: 13487
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: One base pair modified (90: A -> T) to avoid EcoRI site. Mutation made in a less conserved base pair.
star: false
creator: Denis Samuylov
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: pRha
sequence: https://synbiohub.org/public/igem/BBa_K914003_sequence/1

pRha
type: SequenceAnnotation
persistent identity: annotation2188559
display id: annotation2188559
version: 1
top level: BBa_K914003
location: https://synbiohub.org/public/igem/BBa_K914003/annotation2188559/range2188559/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K914003/annotation2188559/range2188559/1
type: Range
persistent identity: range2188559
display id: range2188559
version: 1
top level: BBa_K914003
direction: direction/forward
start: 1
end: 122
orientation: inline

BBa_K1583105
type: ComponentDefinition
persistent identity: BBa K1583105
display id: BBa_K1583105
version: 1
was generated by: iGEM to SBOL conversion
description: pRha + CsgA_Mfp3_His fusion protein
created: 2015-09-03T11:00:00Z
modified: 2015-09-18T04:28:25Z
mutable provenance: This DNA was synthesized. The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1] 1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866. The CsgA sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).
owned by: james
owned by: myers
mutable description: CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms taken from E.coli K-12 MG1655. Inspired by mussels, the Mfp3 (mussel foot protein) has high adhesive properties towards wet polar surfaces.By creating a fusion protein, the adhesive properties of the mussel foot protein is combined with the formation of bacterial nanowires. The design originates from "Strong underwater adhesives made by self-assembling multi-protein nanofibres" C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2000_
m user id: 24478
owner id: 24463
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: A mutation in CsgA had to be introduced to remove a PstI restriction site. This was done without changing the protein sequence and a codon optimizer was used to ensure high expression in E.coli.
star: false
creator: Stefan Robert Marsden
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K914003
component: BBa_K1583062
component: BBa_K1583061
component: BBa_K1583063
component: BBa_K1583059
component: BBa_K1583014
component: BBa_K1583011
component: BBa_K1583012
sequence annotation: BBa_K1583062
sequence annotation: BBa_K914003
sequence annotation: BBa_K1583063
sequence annotation: BBa_K1583061
sequence annotation: BBa_K1583014
sequence annotation: BBa_K1583012
sequence annotation: BBa_K1583011
sequence annotation: BBa_K1583059
sequence: https://synbiohub.org/public/igem/BBa_K1583105_sequence/1

BBa_K914003
type: Component
persistent identity: component2461210
display id: component2461210
version: 1
top level: BBa_K1583105
definition: BBa_K914003
access: public

BBa_K1583062
type: Component
persistent identity: component2461208
display id: component2461208
version: 1
top level: BBa_K1583105
definition: BBa_K1583062
access: public

BBa_K1583061
type: Component
persistent identity: component2461212
display id: component2461212
version: 1
top level: BBa_K1583105
definition: BBa_K1583061
access: public

BBa_K1583063
type: Component
persistent identity: component2461211
display id: component2461211
version: 1
top level: BBa_K1583105
definition: BBa_K1583063
access: public

BBa_K1583059
type: Component
persistent identity: component2461217
display id: component2461217
version: 1
top level: BBa_K1583105
definition: BBa_K1583059
access: public

BBa_K1583014
type: Component
persistent identity: component2461214
display id: component2461214
version: 1
top level: BBa_K1583105
definition: BBa_K1583014
access: public

BBa_K1583062
type: Component
persistent identity: component2461213
display id: component2461213
version: 1
top level: BBa_K1583105
definition: BBa_K1583062
access: public

BBa_K1583011
type: Component
persistent identity: component2461216
display id: component2461216
version: 1
top level: BBa_K1583105
definition: BBa_K1583011
access: public

BBa_K1583012
type: Component
persistent identity: component2461215
display id: component2461215
version: 1
top level: BBa_K1583105
definition: BBa_K1583012
access: public

BBa_K1583062
type: SequenceAnnotation
persistent identity: annotation2461208
display id: annotation2461208
version: 1
top level: BBa_K1583105
location: https://synbiohub.org/public/igem/BBa_K1583105/annotation2461208/range2461208/1
component: BBa_K1583062

https://synbiohub.org/public/igem/BBa_K1583105/annotation2461208/range2461208/1
type: Range
persistent identity: range2461208
display id: range2461208
version: 1
top level: BBa_K1583105
direction: direction/reverse
start: 1
end: 6
orientation: inline

BBa_K914003
type: SequenceAnnotation
persistent identity: annotation2461210
display id: annotation2461210
version: 1
top level: BBa_K1583105
location: https://synbiohub.org/public/igem/BBa_K1583105/annotation2461210/range2461210/1
component: BBa_K914003

https://synbiohub.org/public/igem/BBa_K1583105/annotation2461210/range2461210/1
type: Range
persistent identity: range2461210
display id: range2461210
version: 1
top level: BBa_K1583105
direction: direction/reverse
start: 9
end: 130
orientation: inline

BBa_K1583063
type: SequenceAnnotation
persistent identity: annotation2461211
display id: annotation2461211
version: 1
top level: BBa_K1583105
location: https://synbiohub.org/public/igem/BBa_K1583105/annotation2461211/range2461211/1
component: BBa_K1583063

https://synbiohub.org/public/igem/BBa_K1583105/annotation2461211/range2461211/1
type: Range
persistent identity: range2461211
display id: range2461211
version: 1
top level: BBa_K1583105
direction: direction/reverse
start: 131
end: 157
orientation: inline

BBa_K1583061
type: SequenceAnnotation
persistent identity: annotation2461212
display id: annotation2461212
version: 1
top level: BBa_K1583105
location: https://synbiohub.org/public/igem/BBa_K1583105/annotation2461212/range2461212/1
component: BBa_K1583061

https://synbiohub.org/public/igem/BBa_K1583105/annotation2461212/range2461212/1
type: Range
persistent identity: range2461212
display id: range2461212
version: 1
top level: BBa_K1583105
direction: direction/reverse
start: 158
end: 169
orientation: inline

BBa_K1583062
type: SequenceAnnotation
persistent identity: annotation2461213
display id: annotation2461213
version: 1
top level: BBa_K1583105
location: https://synbiohub.org/public/igem/BBa_K1583105/annotation2461213/range2461213/1
component: BBa_K1583062

https://synbiohub.org/public/igem/BBa_K1583105/annotation2461213/range2461213/1
type: Range
persistent identity: range2461213
display id: range2461213
version: 1
top level: BBa_K1583105
direction: direction/reverse
start: 170
end: 175
orientation: inline

BBa_K1583014
type: SequenceAnnotation
persistent identity: annotation2461214
display id: annotation2461214
version: 1
top level: BBa_K1583105
location: https://synbiohub.org/public/igem/BBa_K1583105/annotation2461214/range2461214/1
component: BBa_K1583014

https://synbiohub.org/public/igem/BBa_K1583105/annotation2461214/range2461214/1
type: Range
persistent identity: range2461214
display id: range2461214
version: 1
top level: BBa_K1583105
direction: direction/reverse
start: 176
end: 628
orientation: inline

BBa_K1583012
type: SequenceAnnotation
persistent identity: annotation2461215
display id: annotation2461215
version: 1
top level: BBa_K1583105
location: https://synbiohub.org/public/igem/BBa_K1583105/annotation2461215/range2461215/1
component: BBa_K1583012

https://synbiohub.org/public/igem/BBa_K1583105/annotation2461215/range2461215/1
type: Range
persistent identity: range2461215
display id: range2461215
version: 1
top level: BBa_K1583105
direction: direction/reverse
start: 629
end: 658
orientation: inline

BBa_K1583011
type: SequenceAnnotation
persistent identity: annotation2461216
display id: annotation2461216
version: 1
top level: BBa_K1583105
location: https://synbiohub.org/public/igem/BBa_K1583105/annotation2461216/range2461216/1
component: BBa_K1583011

https://synbiohub.org/public/igem/BBa_K1583105/annotation2461216/range2461216/1
type: Range
persistent identity: range2461216
display id: range2461216
version: 1
top level: BBa_K1583105
direction: direction/reverse
start: 659
end: 796
orientation: inline

BBa_K1583059
type: SequenceAnnotation
persistent identity: annotation2461217
display id: annotation2461217
version: 1
top level: BBa_K1583105
location: https://synbiohub.org/public/igem/BBa_K1583105/annotation2461217/range2461217/1
component: BBa_K1583059

https://synbiohub.org/public/igem/BBa_K1583105/annotation2461217/range2461217/1
type: Range
persistent identity: range2461217
display id: range2461217
version: 1
top level: BBa_K1583105
direction: direction/reverse
start: 797
end: 817
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

https://synbiohub.org/public/igem/BBa_K1583111_sequence/1
type: Sequence
persistent identity: BBa K1583111 sequence
display id: BBa_K1583111_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatagtagccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaaatactagagcagcaggtacctactagaaaagaggagaaatagtagatgaaactgctgaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgctgcggttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtacggcggtggcggtagcggtggcggtggcagtgctgattattatggtccaaagtatggtcctccaagacgctacggtggtggcaactacaatagatatggcagacgttatggcgggtataaaggctggaacaatggttggaaaagaggtcgatggggacgaaagtattatcatcaccatcaccatcactaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13521_sequence/1
type: Sequence
persistent identity: BBa I13521 sequence
display id: BBa_I13521_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583011_sequence/1
type: Sequence
persistent identity: BBa K1583011 sequence
display id: BBa_K1583011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctgattattatggtccaaagtatggtcctccaagacgctacggtggtggcaactacaatagatatggcagacgttatggcgggtataaaggctggaacaatggttggaaaagaggtcgatggggacgaaagtattat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583012_sequence/1
type: Sequence
persistent identity: BBa K1583012 sequence
display id: BBa_K1583012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcggtggcggtagcggtggcggtggcagt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583059_sequence/1
type: Sequence
persistent identity: BBa K1583059 sequence
display id: BBa_K1583059_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catcaccatcaccatcactaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583061_sequence/1
type: Sequence
persistent identity: BBa K1583061 sequence
display id: BBa_K1583061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583014_sequence/1
type: Sequence
persistent identity: BBa K1583014 sequence
display id: BBa_K1583014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaactgctgaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgctgcggttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583062_sequence/1
type: Sequence
persistent identity: BBa K1583062 sequence
display id: BBa_K1583062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tagtag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583063_sequence/1
type: Sequence
persistent identity: BBa K1583063 sequence
display id: BBa_K1583063_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atactagagcagcaggtacctactaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K914003_sequence/1
type: Sequence
persistent identity: BBa K914003 sequence
display id: BBa_K914003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1583105_sequence/1
type: Sequence
persistent identity: BBa K1583105 sequence
display id: BBa_K1583105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tagtagagccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaaatactagagcagcaggtacctactagaaaagaggagaaatagtagatgaaactgctgaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgctgcggttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtacggcggtggcggtagcggtggcggtggcagtgctgattattatggtccaaagtatggtcctccaagacgctacggtggtggcaactacaatagatatggcagacgttatggcgggtataaaggctggaacaatggttggaaaagaggtcgatggggacgaaagtattatcatcaccatcaccatcactaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z