LOCUS       BBa_K1583112            1521 bp    DNA     linear       26-Nov-2024
DEFINITION  pRha + CsgA & GFP in same operon
ACCESSION   BBa_K1583112
VERSION     BBa_K1583112.1  
FEATURES             Location/Qualifiers
     misc_feature    1..8
                     /label=BBa_G0002
     promoter        9..130
                     /label=pRha
     promoter        9..130
                     /label=BBa_K914003
     misc_feature    131..155
                     /label=Scar
     misc_feature    131..155
                     /label=BBa_K1583050
     RBS             156..167
                     /label=BBa_K1583008
     CDS             169..624
                     /label=CsgA
     CDS             169..624
                     /label=BBa_K1583000
     misc_feature    625..638
                     /label=Scar
     misc_feature    625..638
                     /label=BBa_K1583051
     CDS             1..638
                     /label=BBa_K1583100
     misc_feature    651..654
                     /label=conserved
     RBS             647..658
                     /label=BBa_B0034
     CDS             665..1384
                     /label=GFP protein
     CDS             665..1384
                     /label=BBa_E0040
     stem_loop       1404..1447
                     /label=stem_loop
     misc_feature    1393..1472
                     /label=BBa_B0010
     terminator      1393..1472
                     /label=BBa_B0010
     stem_loop       1488..1507
                     /label=T7 TE
     misc_feature    1514..1514
                     /label=stop
     polyA_site      1508..1521
                     /label=polya
     misc_feature    1481..1521
                     /label=BBa_B0012
     terminator      1481..1521
                     /label=BBa_B0012
     misc_feature    647..1521
                     /label=BBa_I13504
ORIGIN
        1 tactagagcc acaattcagc aaattgtgaa catcatcacg ttcatctttc cctggttgcc
       61 aatggcccat tttcctgtca gtaacgagaa ggtcgcgtat tcaggcgctt tttagactgg
      121 tcgtaatgaa tactagagca gcaaggaaat actagaagga ggtatataat gaaactgctg
      181 aaagtagcag caattgcagc aatcgtattc tccggtagcg ctctggcagg tgttgttcct
      241 cagtacggcg gcggcggtaa ccacggtggt ggcggtaata atagcggccc aaattctgag
      301 ctgaacattt accagtacgg tggcggtaac tctgcacttg ctctgcaaac tgatgcccgt
      361 aactctgact tgactattac ccagcatggc ggcggtaatg gtgcagatgt tggtcagggc
      421 tcagatgaca gctcaatcga tctgacccaa cgtggcttcg gtaacagcgc tactcttgat
      481 cagtggaacg gcaaaaattc tgaaatgacg gttaaacagt tcggtggtgg caacggtgca
      541 gcagttgacc agactgcatc taactcctcc gtcaacgtga ctcaggttgg ctttggtaac
      601 aacgcgaccg ctcatcagta ctaactacta gaaggaggta ctagagaaag aggagaaata
      661 ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
      721 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
      781 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
      841 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa
      901 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
      961 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct
     1021 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca
     1081 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
     1141 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
     1201 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
     1261 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
     1321 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata
     1381 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1441 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     1501 gtgggccttt ctgcgtttat a
//