LOCUS       BBa_K1584026            1848 bp    DNA     linear       22-Dec-2024
DEFINITION  Constitutive LuxR with AHL-inducible GFP
ACCESSION   BBa_K1584026
VERSION     BBa_K1584026.1  
FEATURES             Location/Qualifiers
     RBS             1..12
                     /label=BBa_J61100
     misc_feature    19..20
                     /label=prefix
     misc_feature    192..192
                     /label=T
     misc_feature    510..510
                     /label=A
     CDS             19..768
                     /label=luxR
     misc_feature    19..774
                     /label=BBa_C0062
     misc_feature    775..799
                     /label=Help:Barcodes
     CDS             19..774
                     /label=BBa_C0062
     stem_loop       815..834
                     /label=T7 TE
     misc_feature    841..841
                     /label=stop
     polyA_site      835..848
                     /label=polya
     misc_feature    808..848
                     /label=BBa_B0012
     terminator      808..848
                     /label=BBa_B0012
     stem_loop       869..891
                     /label=stem_loop
     misc_feature    857..902
                     /label=BBa_B0011
     terminator      857..902
                     /label=BBa_B0011
     terminator      808..902
                     /label=BBa_B0014
     terminator      808..902
                     /label=BBa_K823017
     misc_feature    911..930
                     /label=LuxR/HSL
     promoter        930..935
                     /label=-35
     promoter        952..957
                     /label=-10
     misc_feature    963..963
                     /label=start
     misc_feature    911..965
                     /label=BBa_R0062
     promoter        911..965
                     /label=BBa_R0062
     misc_feature    978..981
                     /label=conserved
     RBS             974..985
                     /label=BBa_B0034
     CDS             992..1711
                     /label=GFP protein
     CDS             992..1711
                     /label=BBa_E0040
     stem_loop       1731..1774
                     /label=stem_loop
     misc_feature    1720..1799
                     /label=BBa_B0010
     terminator      1720..1799
                     /label=BBa_B0010
     stem_loop       1815..1834
                     /label=T7 TE
     misc_feature    1841..1841
                     /label=stop
     polyA_site      1835..1848
                     /label=polya
     misc_feature    1808..1848
                     /label=BBa_B0012
     terminator      1808..1848
                     /label=BBa_B0012
     misc_feature    911..1848
                     /label=BBa_J37032
ORIGIN
        1 aaagagggga catactagat gaaaaacata aatgccgacg acacatacag aataattaat
       61 aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa
      121 atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct
      181 gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat
      241 ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc aattaattgg
      301 aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa
      361 acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga
      421 atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg
      481 tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa aataaatata
      541 gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca
      601 tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc
      661 actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt
      721 tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta ataacactga
      781 tagtgctagt gtagatcact actagagtca cactggctca ccttcgggtg ggcctttctg
      841 cgtttatata ctagagagag aatataaaaa gccagattat taatccggct tttttattat
      901 tttactagag acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga
      961 ataaatacta gagaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg
     1021 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
     1081 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
     1141 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg
     1201 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga
     1261 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc
     1321 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt
     1381 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt
     1441 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa
     1501 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
     1561 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
     1621 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
     1681 acatggcatg gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa
     1741 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1801 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//