LOCUS       BBa_K1584035            1205 bp    DNA     linear       22-Dec-2024
DEFINITION  Constitutive LuxR plus AHL-inducible LacZ
ACCESSION   BBa_K1584035
VERSION     BBa_K1584035.1  
FEATURES             Location/Qualifiers
     RBS             1..12
                     /label=BBa_J61100
     misc_feature    19..20
                     /label=prefix
     misc_feature    192..192
                     /label=T
     misc_feature    510..510
                     /label=A
     CDS             19..768
                     /label=luxR
     misc_feature    19..774
                     /label=BBa_C0062
     misc_feature    775..799
                     /label=Help:Barcodes
     CDS             19..774
                     /label=BBa_C0062
     stem_loop       815..834
                     /label=T7 TE
     misc_feature    841..841
                     /label=stop
     polyA_site      835..848
                     /label=polya
     misc_feature    808..848
                     /label=BBa_B0012
     terminator      808..848
                     /label=BBa_B0012
     stem_loop       869..891
                     /label=stem_loop
     misc_feature    857..902
                     /label=BBa_B0011
     terminator      857..902
                     /label=BBa_B0011
     terminator      808..902
                     /label=BBa_B0014
     terminator      808..902
                     /label=BBa_K823017
     misc_feature    911..930
                     /label=LuxR/HSL
     promoter        930..935
                     /label=-35
     promoter        952..957
                     /label=-10
     misc_feature    963..963
                     /label=start
     misc_feature    911..965
                     /label=BBa_R0062
     promoter        911..965
                     /label=BBa_R0062
     CDS             972..1205
                     /label=lacZ
     misc_feature    972..974
                     /label=START
     misc_feature    1200..1205
                     /label=STOP
     CDS             972..1205
                     /label=BBa_I732006
ORIGIN
        1 aaagagggga catactagat gaaaaacata aatgccgacg acacatacag aataattaat
       61 aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa
      121 atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct
      181 gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat
      241 ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc aattaattgg
      301 aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa
      361 acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga
      421 atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg
      481 tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa aataaatata
      541 gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca
      601 tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc
      661 actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt
      721 tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta ataacactga
      781 tagtgctagt gtagatcact actagagtca cactggctca ccttcgggtg ggcctttctg
      841 cgtttatata ctagagagag aatataaaaa gccagattat taatccggct tttttattat
      901 tttactagag acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga
      961 ataaatacta gatgaccatg attacggatt cactggccgt cgttttacaa cgtcgtgact
     1021 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     1081 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     1141 gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt
     1201 aataa
//