BBa_K1585104
1
BBa_K1585104
Anderson Promoter with lacI binding site
2015-08-31T11:00:00Z
2015-09-01T03:26:03Z
Assambly of [http://parts.igem.org/Part:BBa_J23104 J23104] and lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010]
The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207].
Promoters of the Anderson Promoter Collection with lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010] to make them [http://openwetware.org/wiki/IPTG IPTG] iducible.
false
false
_2002_
24474
24474
9
false
We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable.
The part is shipped in backbone [http://parts.igem.org/Part:BBa_J61002 J61002] because the functionality is directly visible by adding IPTG. RFP ([http://parts.igem.org/Part:BBa_E1010 E1010]) should get expressed then.
[[File:K15751xx design.png |600px]]
false
Tobias Schwanemann
component2441422
1
BBa_K1585999
component2441420
1
BBa_J23104
annotation2441422
1
BBa_K1585999
range2441422
1
44
78
annotation2441420
1
BBa_J23104
range2441420
1
1
35
BBa_J23104
1
BBa_J23104
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
isolated from library of promoters
replace later
false
false
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K1585999
1
BBa_K1585999
lacI binding site
2015-08-23T11:00:00Z
2015-08-25T06:18:49Z
lacI-binding-site of [http://parts.igem.org/Part:BBa_R0010 R_0010]
lacI binding site derived from [http://parts.igem.org/Part:BBa_R0010 R_0010]
false
false
_2002_
24474
24474
9
false
The "lacI binding site" of Part [http://parts.igem.org/Part:BBa_R0010 R_0010] differs from lacO from Part [http://parts.igem.org/Part:BBa_J33207 BBa_J33207] although they should be same.
We choosed part BBa_R0010 because we assumed older parts would be reliable.
false
Tobias Schwanemann
annotation2436909
1
lacO/lacI-binding-site
range2436909
1
1
35
BBa_K1585104_sequence
1
ttgacagctagctcagtcctaggtattgtgctagctactagagtgtgtggaattgtgagcggataacaatttcacaca
BBa_K1585999_sequence
1
tgtgtggaattgtgagcggataacaatttcacaca
BBa_J23104_sequence
1
ttgacagctagctcagtcctaggtattgtgctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z