BBa_K1585202 1 BBa_K1585202 PHI 2015-08-24T11:00:00Z 2015-08-28T08:23:36Z Bacillus methanolicus 6-phospho-3-hexuloisomerase from Bacillus methanolicus MGA3, codon optimized for E.coli, converts D-arabino-hex-3-ulose 6-phosphate to D-fructose 6-phosphate false false _2002_ 19522 24470 9 false codon optimized false Carolin Gr??tering annotation2474281 1 phi range2474281 1 1 558 BBa_K1585212 1 BBa_K1585212 B0034.PHI 2015-08-24T11:00:00Z 2015-08-28T08:24:03Z Bacillus methanolicus This is the translational unit of the 6-phospho-3-hexuloisomerase (PHI) from Bacillus methanolicus MGA3. It is codon optimized for E.coli. false false _2002_ 19522 24505 9 false RFC10 compatible false Laura Grabowski component2439456 1 BBa_K1585202 component2439455 1 BBa_B0034 annotation2439455 1 BBa_B0034 range2439455 1 1 12 annotation2439456 1 BBa_K1585202 range2439456 1 19 576 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1585202_sequence 1 atgctgaccaccgaatttctggcggaaattgtgaaagaactgaacagcagcgtgaaccagattgcggatgaagaagcggaagcgctggtgaacggcattctgcaaagcaaaaaagtgtttgtggcgggcgcgggccgcagcggctttatggcgaaaagctttgcgatgcgtatgatgcatatgggcattgatgcgtatgtggtgggcgaaaccgtgaccccgaactatgaaaaagaagatattctgattattggcagcggcagcggcgaaaccaaaagcctggtgagcatggcgcagaaagcgaaaagcattggcggcaccattgcggcggtgaccattaacccggaaagcaccattggccaactggcggatattgtgattaaaatgccgggcagcccgaaagataaaagcgaagcgcgcgaaaccattcagccgatgggcagcctgtttgaacagaccctgctgctgttttatgatgcggtgattctgcgctttatggaaaaaaaaggcctggataccaaaaccatgtatggccgccatgcgaacctggaataataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K1585212_sequence 1 aaagaggagaaatactagatgctgaccaccgaatttctggcggaaattgtgaaagaactgaacagcagcgtgaaccagattgcggatgaagaagcggaagcgctggtgaacggcattctgcaaagcaaaaaagtgtttgtggcgggcgcgggccgcagcggctttatggcgaaaagctttgcgatgcgtatgatgcatatgggcattgatgcgtatgtggtgggcgaaaccgtgaccccgaactatgaaaaagaagatattctgattattggcagcggcagcggcgaaaccaaaagcctggtgagcatggcgcagaaagcgaaaagcattggcggcaccattgcggcggtgaccattaacccggaaagcaccattggccaactggcggatattgtgattaaaatgccgggcagcccgaaagataaaagcgaagcgcgcgaaaccattcagccgatgggcagcctgtttgaacagaccctgctgctgttttatgatgcggtgattctgcgctttatggaaaaaaaaggcctggataccaaaaccatgtatggccgccatgcgaacctggaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z