BBa_K1585221 1 BBa_K1585221 B0034.Hps.B0015 2015-08-27T11:00:00Z 2015-08-28T09:29:57Z synthetic polycistronic expression of methanol assimilation genes false false _2002_ 19522 19522 9 Not in stock false the part is a mix of BioBrick and RDP assembly false Michael Osthege component2439678 1 BBa_B0015 component2439671 1 BBa_K1585211 annotation2439678 1 BBa_B0015 range2439678 1 666 794 annotation2439671 1 BBa_K1585211 range2439671 1 1 657 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K1585201 1 BBa_K1585201 HPS 2015-08-24T11:00:00Z 2015-08-28T08:20:38Z Bacillus methanolicus 3-hexulose-6-phosphate synthase from Hps Bacillus methanolicus MGA3, codon optimized for E.coli false false _2002_ 19522 24470 9 false RFC10 compatible false Carolin Gr??tering annotation2474236 1 hps range2474236 1 1 639 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_K1585211 1 BBa_K1585211 B0034.HPS 2015-08-24T11:00:00Z 2015-08-28T08:21:36Z Bacillus methanolicus MGA3 This is the translational unit of 3-hexulose-6-phosphate synthase (HPS) from Bacillus methanolicus MGA3. It is codon optimized for E.coli. false false _2002_ 19522 24505 9 false RFC10 compatible false Laura Grabowski component2439453 1 BBa_K1585201 component2439452 1 BBa_B0034 annotation2439452 1 BBa_B0034 range2439452 1 1 12 annotation2439453 1 BBa_K1585201 range2439453 1 19 657 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1585221_sequence 1 aaagaggagaaatactagatggaactgcaactggcgctggatctggtgaacattgaagaagcgaaacaggtggtggcggaagtgcaggaatatgtggatattgtggaaattggcaccccggtgattaaaatttggggcctgcaagcggtgaaagcggtgaaagatgcgtttccgcatctgcaagtgctggcggatatgaaaaccatggatgcggcggcgtatgaagtggcgaaagcggcggaacatggcgcggatattgtgaccattctggcggcggcggaagatgtgagcattaaaggcgcggtggaagaagcgaaaaaactgggcaaaaaaattctggtggatatgattgcggtgaaaaacctggaagaacgcgcgaaacaggtggatgaaatgggcgtggattatatttgcgtgcatgcgggttatgatctgcaagccgttggcaagaacccgctggatgatctgaaacgcattaaggcggtggtgaaaaacgcgaaaaccgcgattgcgggcggcattaaactggaaaccctgccggaagtgattaaagcggaaccggatctggtgattgtgggcggcggcattgcgaaccagaccgataaaaaagcggcggcggaaaaaattaacaaactggtgaaacagggcctgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_K1585211_sequence 1 aaagaggagaaatactagatggaactgcaactggcgctggatctggtgaacattgaagaagcgaaacaggtggtggcggaagtgcaggaatatgtggatattgtggaaattggcaccccggtgattaaaatttggggcctgcaagcggtgaaagcggtgaaagatgcgtttccgcatctgcaagtgctggcggatatgaaaaccatggatgcggcggcgtatgaagtggcgaaagcggcggaacatggcgcggatattgtgaccattctggcggcggcggaagatgtgagcattaaaggcgcggtggaagaagcgaaaaaactgggcaaaaaaattctggtggatatgattgcggtgaaaaacctggaagaacgcgcgaaacaggtggatgaaatgggcgtggattatatttgcgtgcatgcgggttatgatctgcaagccgttggcaagaacccgctggatgatctgaaacgcattaaggcggtggtgaaaaacgcgaaaaccgcgattgcgggcggcattaaactggaaaccctgccggaagtgattaaagcggaaccggatctggtgattgtgggcggcggcattgcgaaccagaccgataaaaaagcggcggcggaaaaaattaacaaactggtgaaacagggcctgtaataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1585201_sequence 1 atggaactgcaactggcgctggatctggtgaacattgaagaagcgaaacaggtggtggcggaagtgcaggaatatgtggatattgtggaaattggcaccccggtgattaaaatttggggcctgcaagcggtgaaagcggtgaaagatgcgtttccgcatctgcaagtgctggcggatatgaaaaccatggatgcggcggcgtatgaagtggcgaaagcggcggaacatggcgcggatattgtgaccattctggcggcggcggaagatgtgagcattaaaggcgcggtggaagaagcgaaaaaactgggcaaaaaaattctggtggatatgattgcggtgaaaaacctggaagaacgcgcgaaacaggtggatgaaatgggcgtggattatatttgcgtgcatgcgggttatgatctgcaagccgttggcaagaacccgctggatgatctgaaacgcattaaggcggtggtgaaaaacgcgaaaaccgcgattgcgggcggcattaaactggaaaccctgccggaagtgattaaagcggaaccggatctggtgattgtgggcggcggcattgcgaaccagaccgataaaaaagcggcggcggaaaaaattaacaaactggtgaaacagggcctgtaataa BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z