BBa_K1585210 1 BBa_K1585210 B0034.Mdh2 2015-08-23T11:00:00Z 2015-09-17T02:19:10Z Bacillus methanolicus MGA3 This is the translational unit of methanol dehydrogenase 2 from Bacillus methanolicus MGA3. It is codon optimized for E.coli. The mdh catalyzes the conversion of methanol to formaldehyde. false false _2002_ 24474 24505 9 false RFC10 compatible false Laura Grabowski component2439406 1 BBa_K1585200 component2439404 1 BBa_B0034 annotation2439404 1 BBa_B0034 range2439404 1 1 12 annotation2439406 1 BBa_K1585200 range2439406 1 19 1179 BBa_K1585222 1 BBa_K1585222 B0034.Phi.B0015 2015-08-27T11:00:00Z 2015-08-28T09:40:16Z synthetic B0034.Phi.B0015 false false _2002_ 19522 19522 9 false theoretical false Michael Osthege component2439695 1 BBa_K1585210 component2439702 1 BBa_B0015 annotation2439695 1 BBa_K1585210 range2439695 1 1 1179 annotation2439702 1 BBa_B0015 range2439702 1 1188 1316 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_K1585200 1 BBa_K1585200 Mdh2 2015-08-23T11:00:00Z 2015-08-28T08:04:53Z Bacillus methanolicus MGA3 Methanol dehydrogenase from Bacillus methanolicus MGA3, codon optimized for E.coli. NADH dependent alcohol dehydrogenase that converts methanol to formaldehyde. false false _2002_ 19522 24470 9 false Coding sequence false Carolin Gr??tering annotation2438209 1 Mdh2 range2438209 1 1 1161 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1585200_sequence 1 atgaccaacacccagagcgcgttttttatgccgagcgtgaacctgtttggcgcgggcagcgtgaacgaagtgggcacccgcctggcggatctgggcgtgaaaaaagcgctgctggtgaccgatgcgggcctgcatggcctgggcctgagcgaaaaaattagcagcattattcgcgcggcgggcgtggaagtgagcatttttccgaaagcggaaccgaacccgaccgataaaaacgtggcggaaggcctggaagcgtataacgcggaaaactgcgatagcattgtgaccctgggcggcggcagcagccatgatgcgggcaaagcgattgcgctggtggcggcgaacggcggcaaaattcatgattatgaaggcgtggatgtgagcaaagaaccgatggtgccgctgattgcgattaacaccaccgcgggcaccggcagcgaactgaccaaatttaccattattaccgataccgaacgcaaagtgaaaatggcgattgtggataaacatgtgaccccgaccctgagcattaacgatccggaactgatggtgggcatgccgccgagcctgaccgcggcgaccggtctggatgcgctgacccacgcgatcgaagcgtacgttagcacaggcgccaccccgatcaccgacgcgctggcgattcaggcgatcaaaatcattagcaaatatctgccgcgtgcggtggccaacggtaaagatatcgaagctcgcgaacagatggcgttcgcccagagcctggcgggcatggcgtttaacaacgcgggcctgggctatgtgcatgcgatcgcgcatcaactgggcggcttttataactttccgcatggcgtgtgcaacgcggtgctgctgccgtatgtgtgccgctttaacctgattagcaaagtggaacgctatgcggaaattgcggcgtttctgggtgaaaacgttgatggtctgagcacctacgatgcggccgaaaaagcgatcaaagcgatcgaacgtatggcgaaagacctgaacattccgaaaggctttaaagaactgggcgcgaaagaagaagatattgaaaccctggcgaaaaacgcgatgaaagatgcgtgcgcgctgaccaacccgcgcaaaccgaaactggaagaagtgattcagattattaaaaacgcgatgtaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K1585210_sequence 1 aaagaggagaaatactagatgaccaacacccagagcgcgttttttatgccgagcgtgaacctgtttggcgcgggcagcgtgaacgaagtgggcacccgcctggcggatctgggcgtgaaaaaagcgctgctggtgaccgatgcgggcctgcatggcctgggcctgagcgaaaaaattagcagcattattcgcgcggcgggcgtggaagtgagcatttttccgaaagcggaaccgaacccgaccgataaaaacgtggcggaaggcctggaagcgtataacgcggaaaactgcgatagcattgtgaccctgggcggcggcagcagccatgatgcgggcaaagcgattgcgctggtggcggcgaacggcggcaaaattcatgattatgaaggcgtggatgtgagcaaagaaccgatggtgccgctgattgcgattaacaccaccgcgggcaccggcagcgaactgaccaaatttaccattattaccgataccgaacgcaaagtgaaaatggcgattgtggataaacatgtgaccccgaccctgagcattaacgatccggaactgatggtgggcatgccgccgagcctgaccgcggcgaccggtctggatgcgctgacccacgcgatcgaagcgtacgttagcacaggcgccaccccgatcaccgacgcgctggcgattcaggcgatcaaaatcattagcaaatatctgccgcgtgcggtggccaacggtaaagatatcgaagctcgcgaacagatggcgttcgcccagagcctggcgggcatggcgtttaacaacgcgggcctgggctatgtgcatgcgatcgcgcatcaactgggcggcttttataactttccgcatggcgtgtgcaacgcggtgctgctgccgtatgtgtgccgctttaacctgattagcaaagtggaacgctatgcggaaattgcggcgtttctgggtgaaaacgttgatggtctgagcacctacgatgcggccgaaaaagcgatcaaagcgatcgaacgtatggcgaaagacctgaacattccgaaaggctttaaagaactgggcgcgaaagaagaagatattgaaaccctggcgaaaaacgcgatgaaagatgcgtgcgcgctgaccaacccgcgcaaaccgaaactggaagaagtgattcagattattaaaaacgcgatgtaataa BBa_K1585222_sequence 1 aaagaggagaaatactagatgaccaacacccagagcgcgttttttatgccgagcgtgaacctgtttggcgcgggcagcgtgaacgaagtgggcacccgcctggcggatctgggcgtgaaaaaagcgctgctggtgaccgatgcgggcctgcatggcctgggcctgagcgaaaaaattagcagcattattcgcgcggcgggcgtggaagtgagcatttttccgaaagcggaaccgaacccgaccgataaaaacgtggcggaaggcctggaagcgtataacgcggaaaactgcgatagcattgtgaccctgggcggcggcagcagccatgatgcgggcaaagcgattgcgctggtggcggcgaacggcggcaaaattcatgattatgaaggcgtggatgtgagcaaagaaccgatggtgccgctgattgcgattaacaccaccgcgggcaccggcagcgaactgaccaaatttaccattattaccgataccgaacgcaaagtgaaaatggcgattgtggataaacatgtgaccccgaccctgagcattaacgatccggaactgatggtgggcatgccgccgagcctgaccgcggcgaccggtctggatgcgctgacccacgcgatcgaagcgtacgttagcacaggcgccaccccgatcaccgacgcgctggcgattcaggcgatcaaaatcattagcaaatatctgccgcgtgcggtggccaacggtaaagatatcgaagctcgcgaacagatggcgttcgcccagagcctggcgggcatggcgtttaacaacgcgggcctgggctatgtgcatgcgatcgcgcatcaactgggcggcttttataactttccgcatggcgtgtgcaacgcggtgctgctgccgtatgtgtgccgctttaacctgattagcaaagtggaacgctatgcggaaattgcggcgtttctgggtgaaaacgttgatggtctgagcacctacgatgcggccgaaaaagcgatcaaagcgatcgaacgtatggcgaaagacctgaacattccgaaaggctttaaagaactgggcgcgaaagaagaagatattgaaaccctggcgaaaaacgcgatgaaagatgcgtgcgcgctgaccaacccgcgcaaaccgaaactggaagaagtgattcagattattaaaaacgcgatgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z