BBa_K1585223
1
BBa_K1585223
B0034.Xpk.B0015
2015-08-27T11:00:00Z
2015-08-28T09:26:42Z
assembled from other parts
protein generator of Xpk (phosphoketolase)
false
false
_2002_
19522
19522
9
false
this is a precursor for another part
false
Michael Osthege
component2439647
1
BBa_K1585213
component2439654
1
BBa_B0015
annotation2439654
1
BBa_B0015
range2439654
1
2508
2636
annotation2439647
1
BBa_K1585213
range2439647
1
1
2499
BBa_K1585203
1
BBa_K1585203
XPK
2015-08-24T11:00:00Z
2015-08-28T08:33:31Z
Bifidobacterium adolescentis
phosphoketolase from Bifidobacterium adolescentis, codon optimized for E.coli, converts Xu5p and pi to actp and g3p
false
false
_2002_
19522
24470
9
false
codon optimized for E.coli
false
Carolin Gr??tering
annotation2439475
1
phosphoketolase
range2439475
1
1
2481
BBa_K1585213
1
BBa_K1585213
B0034.XPK
2015-08-24T11:00:00Z
2015-08-28T08:37:34Z
Bifidobacterium adolescentis
This is the translational unit for the phosphoketolase (XPK). It derives from Bifidobacterium adolescentis and is codon optimized for E. coli.
false
false
_2002_
19522
24505
9
false
RFC10 compatible
false
Laura Grabowski
component2439488
1
BBa_K1585203
component2439486
1
BBa_B0034
annotation2439486
1
BBa_B0034
range2439486
1
1
12
annotation2439488
1
BBa_K1585203
range2439488
1
19
2499
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1585213_sequence
1
aaagaggagaaatactagatgaccagcccggtgattggcaccccgtggaaaaaactgaacgcgccggtgagcgaagaagcgattgaaggcgtggataaatattggcgcgcggcgaactatctgagcattggccagatttatctgcgtagcaacccgctgatgaaagaaccgtttacccgcgaagatgtgaaacatcgcctggtgggccattggggcaccaccccgggcctgaactttctgattggccatattaaccgcctgattgcggatcatcagcagaacaccgtgattattatgggcccgggccatggcggcccggcgggcaccgcgcagagctatctggatggcacctataccgaatattttccgaacattaccaaagatgaagcgggcctgcaaaaattttttcgccagtttagctatccgggcggcattccgagccattatgcgccggaaaccccgggcagcattcatgaaggcggcgaactgggctatgcgctgagccatgcgtatggcgcggtgatgaacaacccgagcctgtttgttccggcgattgtgggcgatggcgaagcggaaaccggcccgctggcgaccggctggcagagcaacaaactgattaacccgcgcaccgatggcattgtgctgccgattctgcatctgaacggctataaaattgcgaacccgaccattctgagccgcattagcgatgaagaactgcatgaattttttcatggcatgggctatgaaccgtatgaatttgtggcgggctttgataacgaagatcatctgagcattcatcgccgctttgcggaactgtttgaaaccgtgtttgatgaaatttgcgatattaaagcggcggcgcagaccgatgatatgacccgcccgttttatccgatgattatttttcgcaccccgaaaggctggacctgcccgaaatttattgatggcaaaaaaaccgaaggtagctggcgcagccatcaggtgccgctggcgagcgcgcgcgataccgaagcgcattttgaagtgctgaaaaactggctggaaagctataaaccggaagaactgtttgatgaaaacggcgcggtgaaaccggaagtgaccgcgtttatgccgaccggcgaactgcgtattggcgaaaacccgaacgcgaacggtggtcgtattcgcgaagaactgaaactgccgaaactggaagattatgaagttaaagaagtggccgagtatggtcatggttggggtcaactggaagcgacccgccgcctgggcgtttatacccgcgatattattaaaaacaacccggattctttccgcatcttcggtccggatgaaaccgcctctaaccgcctgcaagcggcctatgatgtgaccaacaaacagtgggatgcgggttatctgtctgcccaggttgatgaacatatggcggtgaccggtcaggttaccgaacaactgagcgaacatcagatggaaggctttctggaaggctatctgctgaccggccgccatggcatctggtctagctatgaaagcttcgtgcatgttatcgattctatgctgaaccagcatgccaaatggctggaagcgaccgttcgcgaaattccgtggcgcaaaccgatctctagcatgaacctgctggttagcagccatgtgtggcgccaggatcataacggctttagccatcaggacccgggcgttaccagcgtgctgctgaacaaatgcttcaataacgaccatgtgatcggcatttattttccggtggatagcaacatgctgctggccgttgcggaaaaatgctataaaagcaccaacaaaattaacgccattattgcgggcaaacaaccggccgcgacctggctgaccctggacgaagcccgcgcggaactggaaaaaggcgccgcggaatggaaatgggcgagcaacgtgaaaagcaacgatgaagcgcagattgtgctggcggcgaccggcgatgtgccgacccaggaaattatggcggcggcggataaactggatgcgatgggcattaaatttaaagtggtgaacgtggtggatctggtgaaactgcaaagcgcgaaagaaaacaacgaagcgctgagcgatgaagaatttgcggaactgtttaccgaagataaaccagtgctgtttgcgtatcatagctatgcgcgcgatgtgcgcggcctgatttatgatcgcccgaaccatgataactttaacgtgcatggctatgaagaacagggcagcaccaccaccccgtatgatatggtgcgcgtgaacaacattgatcgctatgaactgcaagccgaagccctgcgtatgattgatgccgataaatacgccgataagatcaacgaactggaagccttccgccaggaagcgttccagttcgccgttgataacggctatgatcatccggattataccgattgggtgtatagcggcgttaacaccaataagcagggcgcgattagcgccaccgcggcgaccgcgggcgacaacgaataataa
BBa_K1585223_sequence
1
aaagaggagaaatactagatgaccagcccggtgattggcaccccgtggaaaaaactgaacgcgccggtgagcgaagaagcgattgaaggcgtggataaatattggcgcgcggcgaactatctgagcattggccagatttatctgcgtagcaacccgctgatgaaagaaccgtttacccgcgaagatgtgaaacatcgcctggtgggccattggggcaccaccccgggcctgaactttctgattggccatattaaccgcctgattgcggatcatcagcagaacaccgtgattattatgggcccgggccatggcggcccggcgggcaccgcgcagagctatctggatggcacctataccgaatattttccgaacattaccaaagatgaagcgggcctgcaaaaattttttcgccagtttagctatccgggcggcattccgagccattatgcgccggaaaccccgggcagcattcatgaaggcggcgaactgggctatgcgctgagccatgcgtatggcgcggtgatgaacaacccgagcctgtttgttccggcgattgtgggcgatggcgaagcggaaaccggcccgctggcgaccggctggcagagcaacaaactgattaacccgcgcaccgatggcattgtgctgccgattctgcatctgaacggctataaaattgcgaacccgaccattctgagccgcattagcgatgaagaactgcatgaattttttcatggcatgggctatgaaccgtatgaatttgtggcgggctttgataacgaagatcatctgagcattcatcgccgctttgcggaactgtttgaaaccgtgtttgatgaaatttgcgatattaaagcggcggcgcagaccgatgatatgacccgcccgttttatccgatgattatttttcgcaccccgaaaggctggacctgcccgaaatttattgatggcaaaaaaaccgaaggtagctggcgcagccatcaggtgccgctggcgagcgcgcgcgataccgaagcgcattttgaagtgctgaaaaactggctggaaagctataaaccggaagaactgtttgatgaaaacggcgcggtgaaaccggaagtgaccgcgtttatgccgaccggcgaactgcgtattggcgaaaacccgaacgcgaacggtggtcgtattcgcgaagaactgaaactgccgaaactggaagattatgaagttaaagaagtggccgagtatggtcatggttggggtcaactggaagcgacccgccgcctgggcgtttatacccgcgatattattaaaaacaacccggattctttccgcatcttcggtccggatgaaaccgcctctaaccgcctgcaagcggcctatgatgtgaccaacaaacagtgggatgcgggttatctgtctgcccaggttgatgaacatatggcggtgaccggtcaggttaccgaacaactgagcgaacatcagatggaaggctttctggaaggctatctgctgaccggccgccatggcatctggtctagctatgaaagcttcgtgcatgttatcgattctatgctgaaccagcatgccaaatggctggaagcgaccgttcgcgaaattccgtggcgcaaaccgatctctagcatgaacctgctggttagcagccatgtgtggcgccaggatcataacggctttagccatcaggacccgggcgttaccagcgtgctgctgaacaaatgcttcaataacgaccatgtgatcggcatttattttccggtggatagcaacatgctgctggccgttgcggaaaaatgctataaaagcaccaacaaaattaacgccattattgcgggcaaacaaccggccgcgacctggctgaccctggacgaagcccgcgcggaactggaaaaaggcgccgcggaatggaaatgggcgagcaacgtgaaaagcaacgatgaagcgcagattgtgctggcggcgaccggcgatgtgccgacccaggaaattatggcggcggcggataaactggatgcgatgggcattaaatttaaagtggtgaacgtggtggatctggtgaaactgcaaagcgcgaaagaaaacaacgaagcgctgagcgatgaagaatttgcggaactgtttaccgaagataaaccagtgctgtttgcgtatcatagctatgcgcgcgatgtgcgcggcctgatttatgatcgcccgaaccatgataactttaacgtgcatggctatgaagaacagggcagcaccaccaccccgtatgatatggtgcgcgtgaacaacattgatcgctatgaactgcaagccgaagccctgcgtatgattgatgccgataaatacgccgataagatcaacgaactggaagccttccgccaggaagcgttccagttcgccgttgataacggctatgatcatccggattataccgattgggtgtatagcggcgttaacaccaataagcagggcgcgattagcgccaccgcggcgaccgcgggcgacaacgaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1585203_sequence
1
atgaccagcccggtgattggcaccccgtggaaaaaactgaacgcgccggtgagcgaagaagcgattgaaggcgtggataaatattggcgcgcggcgaactatctgagcattggccagatttatctgcgtagcaacccgctgatgaaagaaccgtttacccgcgaagatgtgaaacatcgcctggtgggccattggggcaccaccccgggcctgaactttctgattggccatattaaccgcctgattgcggatcatcagcagaacaccgtgattattatgggcccgggccatggcggcccggcgggcaccgcgcagagctatctggatggcacctataccgaatattttccgaacattaccaaagatgaagcgggcctgcaaaaattttttcgccagtttagctatccgggcggcattccgagccattatgcgccggaaaccccgggcagcattcatgaaggcggcgaactgggctatgcgctgagccatgcgtatggcgcggtgatgaacaacccgagcctgtttgttccggcgattgtgggcgatggcgaagcggaaaccggcccgctggcgaccggctggcagagcaacaaactgattaacccgcgcaccgatggcattgtgctgccgattctgcatctgaacggctataaaattgcgaacccgaccattctgagccgcattagcgatgaagaactgcatgaattttttcatggcatgggctatgaaccgtatgaatttgtggcgggctttgataacgaagatcatctgagcattcatcgccgctttgcggaactgtttgaaaccgtgtttgatgaaatttgcgatattaaagcggcggcgcagaccgatgatatgacccgcccgttttatccgatgattatttttcgcaccccgaaaggctggacctgcccgaaatttattgatggcaaaaaaaccgaaggtagctggcgcagccatcaggtgccgctggcgagcgcgcgcgataccgaagcgcattttgaagtgctgaaaaactggctggaaagctataaaccggaagaactgtttgatgaaaacggcgcggtgaaaccggaagtgaccgcgtttatgccgaccggcgaactgcgtattggcgaaaacccgaacgcgaacggtggtcgtattcgcgaagaactgaaactgccgaaactggaagattatgaagttaaagaagtggccgagtatggtcatggttggggtcaactggaagcgacccgccgcctgggcgtttatacccgcgatattattaaaaacaacccggattctttccgcatcttcggtccggatgaaaccgcctctaaccgcctgcaagcggcctatgatgtgaccaacaaacagtgggatgcgggttatctgtctgcccaggttgatgaacatatggcggtgaccggtcaggttaccgaacaactgagcgaacatcagatggaaggctttctggaaggctatctgctgaccggccgccatggcatctggtctagctatgaaagcttcgtgcatgttatcgattctatgctgaaccagcatgccaaatggctggaagcgaccgttcgcgaaattccgtggcgcaaaccgatctctagcatgaacctgctggttagcagccatgtgtggcgccaggatcataacggctttagccatcaggacccgggcgttaccagcgtgctgctgaacaaatgcttcaataacgaccatgtgatcggcatttattttccggtggatagcaacatgctgctggccgttgcggaaaaatgctataaaagcaccaacaaaattaacgccattattgcgggcaaacaaccggccgcgacctggctgaccctggacgaagcccgcgcggaactggaaaaaggcgccgcggaatggaaatgggcgagcaacgtgaaaagcaacgatgaagcgcagattgtgctggcggcgaccggcgatgtgccgacccaggaaattatggcggcggcggataaactggatgcgatgggcattaaatttaaagtggtgaacgtggtggatctggtgaaactgcaaagcgcgaaagaaaacaacgaagcgctgagcgatgaagaatttgcggaactgtttaccgaagataaaccagtgctgtttgcgtatcatagctatgcgcgcgatgtgcgcggcctgatttatgatcgcccgaaccatgataactttaacgtgcatggctatgaagaacagggcagcaccaccaccccgtatgatatggtgcgcgtgaacaacattgatcgctatgaactgcaagccgaagccctgcgtatgattgatgccgataaatacgccgataagatcaacgaactggaagccttccgccaggaagcgttccagttcgccgttgataacggctatgatcatccggattataccgattgggtgtatagcggcgttaacaccaataagcagggcgcgattagcgccaccgcggcgaccgcgggcgacaacgaataataa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z