BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1585997
1
BBa_K1585997
RDP X-scar
2015-08-27T11:00:00Z
2015-08-28T07:39:17Z
synthetic
This scar is created by RDP-assembly of two X-ends
false
false
_2002_
19522
19522
9
false
-
false
Michael Osthege
BBa_K1585998
1
BBa_K1585998
Promoter-RBS spacer
2015-08-27T11:00:00Z
2015-08-28T07:34:54Z
synthetic
We used this sequence to get the correct spacing between promoter and RBS sequence.
false
false
_2002_
19522
19522
9
false
it resembles the beginning of the RFC10 scar
false
Michael Osthege
BBa_K1585995
1
BBa_K1585995
Trp terminator
2015-08-27T11:00:00Z
2015-08-28T08:07:59Z
synthesized by SynBiota
Terminator from the SynBiota iGEM Kit
false
false
_2002_
19522
19522
9
false
designed by SynBiota
false
Michael Osthege
annotation2439384
1
stop
range2439384
1
1
3
BBa_K1585996
1
BBa_K1585996
RDP Z-scar
2015-08-27T11:00:00Z
2015-08-28T07:41:55Z
synthetic
This scar is created by RDP-assembly of two Z-ends
false
false
_2002_
19522
19522
9
false
-
false
Michael Osthege
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_K1585210
1
BBa_K1585210
B0034.Mdh2
2015-08-23T11:00:00Z
2015-09-17T02:19:10Z
Bacillus methanolicus MGA3
This is the translational unit of methanol dehydrogenase 2 from Bacillus methanolicus MGA3. It is codon optimized for E.coli. The mdh catalyzes the conversion of methanol to formaldehyde.
false
false
_2002_
24474
24505
9
false
RFC10 compatible
false
Laura Grabowski
component2439404
1
BBa_B0034
component2439406
1
BBa_K1585200
annotation2439404
1
BBa_B0034
range2439404
1
1
12
annotation2439406
1
BBa_K1585200
range2439406
1
19
1179
BBa_K1585200
1
BBa_K1585200
Mdh2
2015-08-23T11:00:00Z
2015-08-28T08:04:53Z
Bacillus methanolicus MGA3
Methanol dehydrogenase from Bacillus methanolicus MGA3, codon optimized for E.coli. NADH dependent alcohol dehydrogenase that converts methanol to formaldehyde.
false
false
_2002_
19522
24470
9
false
Coding sequence
false
Carolin Gr??tering
annotation2438209
1
Mdh2
range2438209
1
1
1161
BBa_K1585240
1
BBa_K1585240
AP19.MDH.T
2015-08-27T11:00:00Z
2015-08-28T09:15:55Z
assembled by RDP assembly
constitutive expression part for Mdh2
false
false
_2002_
19522
19522
9
false
RDP-compatible, but the sequence is a mix of BioBrick/RDP standard
false
Michael Osthege
component2439610
1
BBa_K1585997
component2439608
1
BBa_J23119
component2439615
1
BBa_K1585210
component2439618
1
BBa_K1585995
component2439609
1
BBa_K1585998
component2439616
1
BBa_K1585996
annotation2439618
1
BBa_K1585995
range2439618
1
1227
1271
annotation2439609
1
BBa_K1585998
range2439609
1
36
39
annotation2439608
1
BBa_J23119
range2439608
1
1
35
annotation2439616
1
BBa_K1585996
range2439616
1
1223
1226
annotation2439610
1
BBa_K1585997
range2439610
1
40
43
annotation2439615
1
BBa_K1585210
range2439615
1
44
1222
BBa_K1585200_sequence
1
atgaccaacacccagagcgcgttttttatgccgagcgtgaacctgtttggcgcgggcagcgtgaacgaagtgggcacccgcctggcggatctgggcgtgaaaaaagcgctgctggtgaccgatgcgggcctgcatggcctgggcctgagcgaaaaaattagcagcattattcgcgcggcgggcgtggaagtgagcatttttccgaaagcggaaccgaacccgaccgataaaaacgtggcggaaggcctggaagcgtataacgcggaaaactgcgatagcattgtgaccctgggcggcggcagcagccatgatgcgggcaaagcgattgcgctggtggcggcgaacggcggcaaaattcatgattatgaaggcgtggatgtgagcaaagaaccgatggtgccgctgattgcgattaacaccaccgcgggcaccggcagcgaactgaccaaatttaccattattaccgataccgaacgcaaagtgaaaatggcgattgtggataaacatgtgaccccgaccctgagcattaacgatccggaactgatggtgggcatgccgccgagcctgaccgcggcgaccggtctggatgcgctgacccacgcgatcgaagcgtacgttagcacaggcgccaccccgatcaccgacgcgctggcgattcaggcgatcaaaatcattagcaaatatctgccgcgtgcggtggccaacggtaaagatatcgaagctcgcgaacagatggcgttcgcccagagcctggcgggcatggcgtttaacaacgcgggcctgggctatgtgcatgcgatcgcgcatcaactgggcggcttttataactttccgcatggcgtgtgcaacgcggtgctgctgccgtatgtgtgccgctttaacctgattagcaaagtggaacgctatgcggaaattgcggcgtttctgggtgaaaacgttgatggtctgagcacctacgatgcggccgaaaaagcgatcaaagcgatcgaacgtatggcgaaagacctgaacattccgaaaggctttaaagaactgggcgcgaaagaagaagatattgaaaccctggcgaaaaacgcgatgaaagatgcgtgcgcgctgaccaacccgcgcaaaccgaaactggaagaagtgattcagattattaaaaacgcgatgtaataa
BBa_K1585997_sequence
1
gatg
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1585998_sequence
1
tact
BBa_K1585240_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactgatgaaagaggagaaatactagatgaccaacacccagagcgcgttttttatgccgagcgtgaacctgtttggcgcgggcagcgtgaacgaagtgggcacccgcctggcggatctgggcgtgaaaaaagcgctgctggtgaccgatgcgggcctgcatggcctgggcctgagcgaaaaaattagcagcattattcgcgcggcgggcgtggaagtgagcatttttccgaaagcggaaccgaacccgaccgataaaaacgtggcggaaggcctggaagcgtataacgcggaaaactgcgatagcattgtgaccctgggcggcggcagcagccatgatgcgggcaaagcgattgcgctggtggcggcgaacggcggcaaaattcatgattatgaaggcgtggatgtgagcaaagaaccgatggtgccgctgattgcgattaacaccaccgcgggcaccggcagcgaactgaccaaatttaccattattaccgataccgaacgcaaagtgaaaatggcgattgtggataaacatgtgaccccgaccctgagcattaacgatccggaactgatggtgggcatgccgccgagcctgaccgcggcgaccggtctggatgcgctgacccacgcgatcgaagcgtacgttagcacaggcgccaccccgatcaccgacgcgctggcgattcaggcgatcaaaatcattagcaaatatctgccgcgtgcggtggccaacggtaaagatatcgaagctcgcgaacagatggcgttcgcccagagcctggcgggcatggcgtttaacaacgcgggcctgggctatgtgcatgcgatcgcgcatcaactgggcggcttttataactttccgcatggcgtgtgcaacgcggtgctgctgccgtatgtgtgccgctttaacctgattagcaaagtggaacgctatgcggaaattgcggcgtttctgggtgaaaacgttgatggtctgagcacctacgatgcggccgaaaaagcgatcaaagcgatcgaacgtatggcgaaagacctgaacattccgaaaggctttaaagaactgggcgcgaaagaagaagatattgaaaccctggcgaaaaacgcgatgaaagatgcgtgcgcgctgaccaacccgcgcaaaccgaaactggaagaagtgattcagattattaaaaacgcgatgtaataacggctgatgagggtcgggcggattactcgcccgaaaaaaaacttgtttt
BBa_K1585996_sequence
1
cggc
BBa_K1585210_sequence
1
aaagaggagaaatactagatgaccaacacccagagcgcgttttttatgccgagcgtgaacctgtttggcgcgggcagcgtgaacgaagtgggcacccgcctggcggatctgggcgtgaaaaaagcgctgctggtgaccgatgcgggcctgcatggcctgggcctgagcgaaaaaattagcagcattattcgcgcggcgggcgtggaagtgagcatttttccgaaagcggaaccgaacccgaccgataaaaacgtggcggaaggcctggaagcgtataacgcggaaaactgcgatagcattgtgaccctgggcggcggcagcagccatgatgcgggcaaagcgattgcgctggtggcggcgaacggcggcaaaattcatgattatgaaggcgtggatgtgagcaaagaaccgatggtgccgctgattgcgattaacaccaccgcgggcaccggcagcgaactgaccaaatttaccattattaccgataccgaacgcaaagtgaaaatggcgattgtggataaacatgtgaccccgaccctgagcattaacgatccggaactgatggtgggcatgccgccgagcctgaccgcggcgaccggtctggatgcgctgacccacgcgatcgaagcgtacgttagcacaggcgccaccccgatcaccgacgcgctggcgattcaggcgatcaaaatcattagcaaatatctgccgcgtgcggtggccaacggtaaagatatcgaagctcgcgaacagatggcgttcgcccagagcctggcgggcatggcgtttaacaacgcgggcctgggctatgtgcatgcgatcgcgcatcaactgggcggcttttataactttccgcatggcgtgtgcaacgcggtgctgctgccgtatgtgtgccgctttaacctgattagcaaagtggaacgctatgcggaaattgcggcgtttctgggtgaaaacgttgatggtctgagcacctacgatgcggccgaaaaagcgatcaaagcgatcgaacgtatggcgaaagacctgaacattccgaaaggctttaaagaactgggcgcgaaagaagaagatattgaaaccctggcgaaaaacgcgatgaaagatgcgtgcgcgctgaccaacccgcgcaaaccgaaactggaagaagtgattcagattattaaaaacgcgatgtaataa
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
BBa_K1585995_sequence
1
tgatgagggtcgggcggattactcgcccgaaaaaaaacttgtttt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z