BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1585997 1 BBa_K1585997 RDP X-scar 2015-08-27T11:00:00Z 2015-08-28T07:39:17Z synthetic This scar is created by RDP-assembly of two X-ends false false _2002_ 19522 19522 9 false - false Michael Osthege BBa_K1585998 1 BBa_K1585998 Promoter-RBS spacer 2015-08-27T11:00:00Z 2015-08-28T07:34:54Z synthetic We used this sequence to get the correct spacing between promoter and RBS sequence. false false _2002_ 19522 19522 9 false it resembles the beginning of the RFC10 scar false Michael Osthege BBa_K1585995 1 BBa_K1585995 Trp terminator 2015-08-27T11:00:00Z 2015-08-28T08:07:59Z synthesized by SynBiota Terminator from the SynBiota iGEM Kit false false _2002_ 19522 19522 9 false designed by SynBiota false Michael Osthege annotation2439384 1 stop range2439384 1 1 3 BBa_K1585996 1 BBa_K1585996 RDP Z-scar 2015-08-27T11:00:00Z 2015-08-28T07:41:55Z synthetic This scar is created by RDP-assembly of two Z-ends false false _2002_ 19522 19522 9 false - false Michael Osthege BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_K1585210 1 BBa_K1585210 B0034.Mdh2 2015-08-23T11:00:00Z 2015-09-17T02:19:10Z Bacillus methanolicus MGA3 This is the translational unit of methanol dehydrogenase 2 from Bacillus methanolicus MGA3. It is codon optimized for E.coli. The mdh catalyzes the conversion of methanol to formaldehyde. false false _2002_ 24474 24505 9 false RFC10 compatible false Laura Grabowski component2439404 1 BBa_B0034 component2439406 1 BBa_K1585200 annotation2439404 1 BBa_B0034 range2439404 1 1 12 annotation2439406 1 BBa_K1585200 range2439406 1 19 1179 BBa_K1585200 1 BBa_K1585200 Mdh2 2015-08-23T11:00:00Z 2015-08-28T08:04:53Z Bacillus methanolicus MGA3 Methanol dehydrogenase from Bacillus methanolicus MGA3, codon optimized for E.coli. NADH dependent alcohol dehydrogenase that converts methanol to formaldehyde. false false _2002_ 19522 24470 9 false Coding sequence false Carolin Gr??tering annotation2438209 1 Mdh2 range2438209 1 1 1161 BBa_K1585240 1 BBa_K1585240 AP19.MDH.T 2015-08-27T11:00:00Z 2015-08-28T09:15:55Z assembled by RDP assembly constitutive expression part for Mdh2 false false _2002_ 19522 19522 9 false RDP-compatible, but the sequence is a mix of BioBrick/RDP standard false Michael Osthege component2439610 1 BBa_K1585997 component2439608 1 BBa_J23119 component2439615 1 BBa_K1585210 component2439618 1 BBa_K1585995 component2439609 1 BBa_K1585998 component2439616 1 BBa_K1585996 annotation2439618 1 BBa_K1585995 range2439618 1 1227 1271 annotation2439609 1 BBa_K1585998 range2439609 1 36 39 annotation2439608 1 BBa_J23119 range2439608 1 1 35 annotation2439616 1 BBa_K1585996 range2439616 1 1223 1226 annotation2439610 1 BBa_K1585997 range2439610 1 40 43 annotation2439615 1 BBa_K1585210 range2439615 1 44 1222 BBa_K1585200_sequence 1 atgaccaacacccagagcgcgttttttatgccgagcgtgaacctgtttggcgcgggcagcgtgaacgaagtgggcacccgcctggcggatctgggcgtgaaaaaagcgctgctggtgaccgatgcgggcctgcatggcctgggcctgagcgaaaaaattagcagcattattcgcgcggcgggcgtggaagtgagcatttttccgaaagcggaaccgaacccgaccgataaaaacgtggcggaaggcctggaagcgtataacgcggaaaactgcgatagcattgtgaccctgggcggcggcagcagccatgatgcgggcaaagcgattgcgctggtggcggcgaacggcggcaaaattcatgattatgaaggcgtggatgtgagcaaagaaccgatggtgccgctgattgcgattaacaccaccgcgggcaccggcagcgaactgaccaaatttaccattattaccgataccgaacgcaaagtgaaaatggcgattgtggataaacatgtgaccccgaccctgagcattaacgatccggaactgatggtgggcatgccgccgagcctgaccgcggcgaccggtctggatgcgctgacccacgcgatcgaagcgtacgttagcacaggcgccaccccgatcaccgacgcgctggcgattcaggcgatcaaaatcattagcaaatatctgccgcgtgcggtggccaacggtaaagatatcgaagctcgcgaacagatggcgttcgcccagagcctggcgggcatggcgtttaacaacgcgggcctgggctatgtgcatgcgatcgcgcatcaactgggcggcttttataactttccgcatggcgtgtgcaacgcggtgctgctgccgtatgtgtgccgctttaacctgattagcaaagtggaacgctatgcggaaattgcggcgtttctgggtgaaaacgttgatggtctgagcacctacgatgcggccgaaaaagcgatcaaagcgatcgaacgtatggcgaaagacctgaacattccgaaaggctttaaagaactgggcgcgaaagaagaagatattgaaaccctggcgaaaaacgcgatgaaagatgcgtgcgcgctgaccaacccgcgcaaaccgaaactggaagaagtgattcagattattaaaaacgcgatgtaataa BBa_K1585997_sequence 1 gatg BBa_B0034_sequence 1 aaagaggagaaa BBa_K1585998_sequence 1 tact BBa_K1585240_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactgatgaaagaggagaaatactagatgaccaacacccagagcgcgttttttatgccgagcgtgaacctgtttggcgcgggcagcgtgaacgaagtgggcacccgcctggcggatctgggcgtgaaaaaagcgctgctggtgaccgatgcgggcctgcatggcctgggcctgagcgaaaaaattagcagcattattcgcgcggcgggcgtggaagtgagcatttttccgaaagcggaaccgaacccgaccgataaaaacgtggcggaaggcctggaagcgtataacgcggaaaactgcgatagcattgtgaccctgggcggcggcagcagccatgatgcgggcaaagcgattgcgctggtggcggcgaacggcggcaaaattcatgattatgaaggcgtggatgtgagcaaagaaccgatggtgccgctgattgcgattaacaccaccgcgggcaccggcagcgaactgaccaaatttaccattattaccgataccgaacgcaaagtgaaaatggcgattgtggataaacatgtgaccccgaccctgagcattaacgatccggaactgatggtgggcatgccgccgagcctgaccgcggcgaccggtctggatgcgctgacccacgcgatcgaagcgtacgttagcacaggcgccaccccgatcaccgacgcgctggcgattcaggcgatcaaaatcattagcaaatatctgccgcgtgcggtggccaacggtaaagatatcgaagctcgcgaacagatggcgttcgcccagagcctggcgggcatggcgtttaacaacgcgggcctgggctatgtgcatgcgatcgcgcatcaactgggcggcttttataactttccgcatggcgtgtgcaacgcggtgctgctgccgtatgtgtgccgctttaacctgattagcaaagtggaacgctatgcggaaattgcggcgtttctgggtgaaaacgttgatggtctgagcacctacgatgcggccgaaaaagcgatcaaagcgatcgaacgtatggcgaaagacctgaacattccgaaaggctttaaagaactgggcgcgaaagaagaagatattgaaaccctggcgaaaaacgcgatgaaagatgcgtgcgcgctgaccaacccgcgcaaaccgaaactggaagaagtgattcagattattaaaaacgcgatgtaataacggctgatgagggtcgggcggattactcgcccgaaaaaaaacttgtttt BBa_K1585996_sequence 1 cggc BBa_K1585210_sequence 1 aaagaggagaaatactagatgaccaacacccagagcgcgttttttatgccgagcgtgaacctgtttggcgcgggcagcgtgaacgaagtgggcacccgcctggcggatctgggcgtgaaaaaagcgctgctggtgaccgatgcgggcctgcatggcctgggcctgagcgaaaaaattagcagcattattcgcgcggcgggcgtggaagtgagcatttttccgaaagcggaaccgaacccgaccgataaaaacgtggcggaaggcctggaagcgtataacgcggaaaactgcgatagcattgtgaccctgggcggcggcagcagccatgatgcgggcaaagcgattgcgctggtggcggcgaacggcggcaaaattcatgattatgaaggcgtggatgtgagcaaagaaccgatggtgccgctgattgcgattaacaccaccgcgggcaccggcagcgaactgaccaaatttaccattattaccgataccgaacgcaaagtgaaaatggcgattgtggataaacatgtgaccccgaccctgagcattaacgatccggaactgatggtgggcatgccgccgagcctgaccgcggcgaccggtctggatgcgctgacccacgcgatcgaagcgtacgttagcacaggcgccaccccgatcaccgacgcgctggcgattcaggcgatcaaaatcattagcaaatatctgccgcgtgcggtggccaacggtaaagatatcgaagctcgcgaacagatggcgttcgcccagagcctggcgggcatggcgtttaacaacgcgggcctgggctatgtgcatgcgatcgcgcatcaactgggcggcttttataactttccgcatggcgtgtgcaacgcggtgctgctgccgtatgtgtgccgctttaacctgattagcaaagtggaacgctatgcggaaattgcggcgtttctgggtgaaaacgttgatggtctgagcacctacgatgcggccgaaaaagcgatcaaagcgatcgaacgtatggcgaaagacctgaacattccgaaaggctttaaagaactgggcgcgaaagaagaagatattgaaaccctggcgaaaaacgcgatgaaagatgcgtgcgcgctgaccaacccgcgcaaaccgaaactggaagaagtgattcagattattaaaaacgcgatgtaataa BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc BBa_K1585995_sequence 1 tgatgagggtcgggcggattactcgcccgaaaaaaaacttgtttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z