LOCUS BBa_K1585241 5103 bp DNA linear 22-Dec-2024 DEFINITION AP19.Mdh2.Hps.Phi.Xpk.B0015 ACCESSION BBa_K1585241 VERSION BBa_K1585241.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23119 misc_feature 36..39 /label=BBa_K1585998 misc_feature 40..43 /label=BBa_K1585997 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..1222 /label=Mdh2 CDS 62..1222 /label=BBa_K1585200 misc_feature 44..1222 /label=BBa_K1585210 misc_feature 1223..1226 /label=BBa_K1585996 misc_feature 1231..1234 /label=conserved RBS 1227..1238 /label=BBa_B0034 CDS 1245..1883 /label=hps CDS 1245..1883 /label=BBa_K1585201 misc_feature 1227..1883 /label=BBa_K1585211 misc_feature 1884..1887 /label=BBa_K1585997 misc_feature 1892..1895 /label=conserved RBS 1888..1899 /label=BBa_B0034 CDS 1906..2463 /label=phi CDS 1906..2463 /label=BBa_K1585202 misc_feature 1888..2463 /label=BBa_K1585212 misc_feature 2464..2467 /label=BBa_K1585996 misc_feature 2472..2475 /label=conserved RBS 2468..2479 /label=BBa_B0034 CDS 2486..4966 /label=phosphoketolase CDS 2486..4966 /label=BBa_K1585203 misc_feature 2468..4966 /label=BBa_K1585213 stem_loop 4986..5029 /label=stem_loop misc_feature 4975..5054 /label=BBa_B0010 terminator 4975..5054 /label=BBa_B0010 stem_loop 5070..5089 /label=T7 TE misc_feature 5096..5096 /label=stop polyA_site 5090..5103 /label=polya misc_feature 5063..5103 /label=BBa_B0012 terminator 5063..5103 /label=BBa_B0012 terminator 4975..5103 /label=BBa_B0015 misc_feature 2468..5103 /label=BBa_K1585223 ORIGIN 1 ttgacagcta gctcagtcct aggtataatg ctagctactg atgaaagagg agaaatacta 61 gatgaccaac acccagagcg cgttttttat gccgagcgtg aacctgtttg gcgcgggcag 121 cgtgaacgaa gtgggcaccc gcctggcgga tctgggcgtg aaaaaagcgc tgctggtgac 181 cgatgcgggc ctgcatggcc tgggcctgag cgaaaaaatt agcagcatta ttcgcgcggc 241 gggcgtggaa gtgagcattt ttccgaaagc ggaaccgaac ccgaccgata aaaacgtggc 301 ggaaggcctg gaagcgtata acgcggaaaa ctgcgatagc attgtgaccc tgggcggcgg 361 cagcagccat gatgcgggca aagcgattgc gctggtggcg gcgaacggcg gcaaaattca 421 tgattatgaa ggcgtggatg tgagcaaaga accgatggtg ccgctgattg cgattaacac 481 caccgcgggc accggcagcg aactgaccaa atttaccatt attaccgata ccgaacgcaa 541 agtgaaaatg gcgattgtgg ataaacatgt gaccccgacc ctgagcatta acgatccgga 601 actgatggtg ggcatgccgc cgagcctgac cgcggcgacc ggtctggatg cgctgaccca 661 cgcgatcgaa gcgtacgtta gcacaggcgc caccccgatc accgacgcgc tggcgattca 721 ggcgatcaaa atcattagca aatatctgcc gcgtgcggtg gccaacggta aagatatcga 781 agctcgcgaa cagatggcgt tcgcccagag cctggcgggc atggcgttta acaacgcggg 841 cctgggctat gtgcatgcga tcgcgcatca actgggcggc ttttataact ttccgcatgg 901 cgtgtgcaac gcggtgctgc tgccgtatgt gtgccgcttt aacctgatta gcaaagtgga 961 acgctatgcg gaaattgcgg cgtttctggg tgaaaacgtt gatggtctga gcacctacga 1021 tgcggccgaa aaagcgatca aagcgatcga acgtatggcg aaagacctga acattccgaa 1081 aggctttaaa gaactgggcg cgaaagaaga agatattgaa accctggcga aaaacgcgat 1141 gaaagatgcg tgcgcgctga ccaacccgcg caaaccgaaa ctggaagaag tgattcagat 1201 tattaaaaac gcgatgtaat aacggcaaag aggagaaata ctagatggaa ctgcaactgg 1261 cgctggatct ggtgaacatt gaagaagcga aacaggtggt ggcggaagtg caggaatatg 1321 tggatattgt ggaaattggc accccggtga ttaaaatttg gggcctgcaa gcggtgaaag 1381 cggtgaaaga tgcgtttccg catctgcaag tgctggcgga tatgaaaacc atggatgcgg 1441 cggcgtatga agtggcgaaa gcggcggaac atggcgcgga tattgtgacc attctggcgg 1501 cggcggaaga tgtgagcatt aaaggcgcgg tggaagaagc gaaaaaactg ggcaaaaaaa 1561 ttctggtgga tatgattgcg gtgaaaaacc tggaagaacg cgcgaaacag gtggatgaaa 1621 tgggcgtgga ttatatttgc gtgcatgcgg gttatgatct gcaagccgtt ggcaagaacc 1681 cgctggatga tctgaaacgc attaaggcgg tggtgaaaaa cgcgaaaacc gcgattgcgg 1741 gcggcattaa actggaaacc ctgccggaag tgattaaagc ggaaccggat ctggtgattg 1801 tgggcggcgg cattgcgaac cagaccgata aaaaagcggc ggcggaaaaa attaacaaac 1861 tggtgaaaca gggcctgtaa taagatgaaa gaggagaaat actagatgct gaccaccgaa 1921 tttctggcgg aaattgtgaa agaactgaac agcagcgtga accagattgc ggatgaagaa 1981 gcggaagcgc tggtgaacgg cattctgcaa agcaaaaaag tgtttgtggc gggcgcgggc 2041 cgcagcggct ttatggcgaa aagctttgcg atgcgtatga tgcatatggg cattgatgcg 2101 tatgtggtgg gcgaaaccgt gaccccgaac tatgaaaaag aagatattct gattattggc 2161 agcggcagcg gcgaaaccaa aagcctggtg agcatggcgc agaaagcgaa aagcattggc 2221 ggcaccattg cggcggtgac cattaacccg gaaagcacca ttggccaact ggcggatatt 2281 gtgattaaaa tgccgggcag cccgaaagat aaaagcgaag cgcgcgaaac cattcagccg 2341 atgggcagcc tgtttgaaca gaccctgctg ctgttttatg atgcggtgat tctgcgcttt 2401 atggaaaaaa aaggcctgga taccaaaacc atgtatggcc gccatgcgaa cctggaataa 2461 taacggcaaa gaggagaaat actagatgac cagcccggtg attggcaccc cgtggaaaaa 2521 actgaacgcg ccggtgagcg aagaagcgat tgaaggcgtg gataaatatt ggcgcgcggc 2581 gaactatctg agcattggcc agatttatct gcgtagcaac ccgctgatga aagaaccgtt 2641 tacccgcgaa gatgtgaaac atcgcctggt gggccattgg ggcaccaccc cgggcctgaa 2701 ctttctgatt ggccatatta accgcctgat tgcggatcat cagcagaaca ccgtgattat 2761 tatgggcccg ggccatggcg gcccggcggg caccgcgcag agctatctgg atggcaccta 2821 taccgaatat tttccgaaca ttaccaaaga tgaagcgggc ctgcaaaaat tttttcgcca 2881 gtttagctat ccgggcggca ttccgagcca ttatgcgccg gaaaccccgg gcagcattca 2941 tgaaggcggc gaactgggct atgcgctgag ccatgcgtat ggcgcggtga tgaacaaccc 3001 gagcctgttt gttccggcga ttgtgggcga tggcgaagcg gaaaccggcc cgctggcgac 3061 cggctggcag agcaacaaac tgattaaccc gcgcaccgat ggcattgtgc tgccgattct 3121 gcatctgaac ggctataaaa ttgcgaaccc gaccattctg agccgcatta gcgatgaaga 3181 actgcatgaa ttttttcatg gcatgggcta tgaaccgtat gaatttgtgg cgggctttga 3241 taacgaagat catctgagca ttcatcgccg ctttgcggaa ctgtttgaaa ccgtgtttga 3301 tgaaatttgc gatattaaag cggcggcgca gaccgatgat atgacccgcc cgttttatcc 3361 gatgattatt tttcgcaccc cgaaaggctg gacctgcccg aaatttattg atggcaaaaa 3421 aaccgaaggt agctggcgca gccatcaggt gccgctggcg agcgcgcgcg ataccgaagc 3481 gcattttgaa gtgctgaaaa actggctgga aagctataaa ccggaagaac tgtttgatga 3541 aaacggcgcg gtgaaaccgg aagtgaccgc gtttatgccg accggcgaac tgcgtattgg 3601 cgaaaacccg aacgcgaacg gtggtcgtat tcgcgaagaa ctgaaactgc cgaaactgga 3661 agattatgaa gttaaagaag tggccgagta tggtcatggt tggggtcaac tggaagcgac 3721 ccgccgcctg ggcgtttata cccgcgatat tattaaaaac aacccggatt ctttccgcat 3781 cttcggtccg gatgaaaccg cctctaaccg cctgcaagcg gcctatgatg tgaccaacaa 3841 acagtgggat gcgggttatc tgtctgccca ggttgatgaa catatggcgg tgaccggtca 3901 ggttaccgaa caactgagcg aacatcagat ggaaggcttt ctggaaggct atctgctgac 3961 cggccgccat ggcatctggt ctagctatga aagcttcgtg catgttatcg attctatgct 4021 gaaccagcat gccaaatggc tggaagcgac cgttcgcgaa attccgtggc gcaaaccgat 4081 ctctagcatg aacctgctgg ttagcagcca tgtgtggcgc caggatcata acggctttag 4141 ccatcaggac ccgggcgtta ccagcgtgct gctgaacaaa tgcttcaata acgaccatgt 4201 gatcggcatt tattttccgg tggatagcaa catgctgctg gccgttgcgg aaaaatgcta 4261 taaaagcacc aacaaaatta acgccattat tgcgggcaaa caaccggccg cgacctggct 4321 gaccctggac gaagcccgcg cggaactgga aaaaggcgcc gcggaatgga aatgggcgag 4381 caacgtgaaa agcaacgatg aagcgcagat tgtgctggcg gcgaccggcg atgtgccgac 4441 ccaggaaatt atggcggcgg cggataaact ggatgcgatg ggcattaaat ttaaagtggt 4501 gaacgtggtg gatctggtga aactgcaaag cgcgaaagaa aacaacgaag cgctgagcga 4561 tgaagaattt gcggaactgt ttaccgaaga taaaccagtg ctgtttgcgt atcatagcta 4621 tgcgcgcgat gtgcgcggcc tgatttatga tcgcccgaac catgataact ttaacgtgca 4681 tggctatgaa gaacagggca gcaccaccac cccgtatgat atggtgcgcg tgaacaacat 4741 tgatcgctat gaactgcaag ccgaagccct gcgtatgatt gatgccgata aatacgccga 4801 taagatcaac gaactggaag ccttccgcca ggaagcgttc cagttcgccg ttgataacgg 4861 ctatgatcat ccggattata ccgattgggt gtatagcggc gttaacacca ataagcaggg 4921 cgcgattagc gccaccgcgg cgaccgcggg cgacaacgaa taataatact agagccaggc 4981 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 5041 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 5101 ata //