BBa_K1585241
type: ComponentDefinition
persistent identity: BBa K1585241
display id: BBa_K1585241
version: 1
was generated by: iGEM to SBOL conversion
description: AP19.Mdh2.Hps.Phi.Xpk.B0015
created: 2015-08-27T11:00:00Z
modified: 2015-09-18T06:08:30Z
mutable provenance: all single parts were synthesized
owned by: james
owned by: myers
mutable description: polycistronic expression part for methanol assimilation genes
discontinued: false
dominant: false
experience: experience/None
group u list: _2002_
m user id: 24505
owner id: 19522
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: the part is a mix of BioBrick and RDP assembly
star: false
creator: Michael Osthege
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1585996
component: BBa_K1585212
component: BBa_K1585997
component: BBa_K1585211
component: BBa_K1585223
component: BBa_J23119
component: BBa_K1585210
component: BBa_K1585998
sequence annotation: BBa_J23119
sequence annotation: BBa_K1585998
sequence annotation: BBa_K1585997
sequence annotation: BBa_K1585210
sequence annotation: BBa_K1585996
sequence annotation: BBa_K1585211
sequence annotation: BBa_K1585212
sequence annotation: BBa_K1585223
sequence: https://synbiohub.org/public/igem/BBa_K1585241_sequence/1

BBa_K1585996
type: Component
persistent identity: component2439792
display id: component2439792
version: 1
top level: BBa_K1585241
definition: BBa_K1585996
access: public

BBa_K1585212
type: Component
persistent identity: component2439791
display id: component2439791
version: 1
top level: BBa_K1585241
definition: BBa_K1585212
access: public

BBa_K1585997
type: Component
persistent identity: component2439787
display id: component2439787
version: 1
top level: BBa_K1585241
definition: BBa_K1585997
access: public

BBa_K1585211
type: Component
persistent identity: component2439786
display id: component2439786
version: 1
top level: BBa_K1585241
definition: BBa_K1585211
access: public

BBa_K1585223
type: Component
persistent identity: component2439805
display id: component2439805
version: 1
top level: BBa_K1585241
definition: BBa_K1585223
access: public

BBa_J23119
type: Component
persistent identity: component2439774
display id: component2439774
version: 1
top level: BBa_K1585241
definition: BBa_J23119
access: public

BBa_K1585996
type: Component
persistent identity: component2439782
display id: component2439782
version: 1
top level: BBa_K1585241
definition: BBa_K1585996
access: public

BBa_K1585210
type: Component
persistent identity: component2439781
display id: component2439781
version: 1
top level: BBa_K1585241
definition: BBa_K1585210
access: public

BBa_K1585997
type: Component
persistent identity: component2439776
display id: component2439776
version: 1
top level: BBa_K1585241
definition: BBa_K1585997
access: public

BBa_K1585998
type: Component
persistent identity: component2439775
display id: component2439775
version: 1
top level: BBa_K1585241
definition: BBa_K1585998
access: public

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2439774
display id: annotation2439774
version: 1
top level: BBa_K1585241
location: https://synbiohub.org/public/igem/BBa_K1585241/annotation2439774/range2439774/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K1585241/annotation2439774/range2439774/1
type: Range
persistent identity: range2439774
display id: range2439774
version: 1
top level: BBa_K1585241
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K1585998
type: SequenceAnnotation
persistent identity: annotation2439775
display id: annotation2439775
version: 1
top level: BBa_K1585241
location: https://synbiohub.org/public/igem/BBa_K1585241/annotation2439775/range2439775/1
component: BBa_K1585998

https://synbiohub.org/public/igem/BBa_K1585241/annotation2439775/range2439775/1
type: Range
persistent identity: range2439775
display id: range2439775
version: 1
top level: BBa_K1585241
direction: direction/reverse
start: 36
end: 39
orientation: inline

BBa_K1585997
type: SequenceAnnotation
persistent identity: annotation2439776
display id: annotation2439776
version: 1
top level: BBa_K1585241
location: https://synbiohub.org/public/igem/BBa_K1585241/annotation2439776/range2439776/1
component: BBa_K1585997

https://synbiohub.org/public/igem/BBa_K1585241/annotation2439776/range2439776/1
type: Range
persistent identity: range2439776
display id: range2439776
version: 1
top level: BBa_K1585241
direction: direction/reverse
start: 40
end: 43
orientation: inline

BBa_K1585210
type: SequenceAnnotation
persistent identity: annotation2439781
display id: annotation2439781
version: 1
top level: BBa_K1585241
location: https://synbiohub.org/public/igem/BBa_K1585241/annotation2439781/range2439781/1
component: BBa_K1585210

https://synbiohub.org/public/igem/BBa_K1585241/annotation2439781/range2439781/1
type: Range
persistent identity: range2439781
display id: range2439781
version: 1
top level: BBa_K1585241
direction: direction/reverse
start: 44
end: 1222
orientation: inline

BBa_K1585996
type: SequenceAnnotation
persistent identity: annotation2439782
display id: annotation2439782
version: 1
top level: BBa_K1585241
location: https://synbiohub.org/public/igem/BBa_K1585241/annotation2439782/range2439782/1
component: BBa_K1585996

https://synbiohub.org/public/igem/BBa_K1585241/annotation2439782/range2439782/1
type: Range
persistent identity: range2439782
display id: range2439782
version: 1
top level: BBa_K1585241
direction: direction/reverse
start: 1223
end: 1226
orientation: inline

BBa_K1585211
type: SequenceAnnotation
persistent identity: annotation2439786
display id: annotation2439786
version: 1
top level: BBa_K1585241
location: https://synbiohub.org/public/igem/BBa_K1585241/annotation2439786/range2439786/1
component: BBa_K1585211

https://synbiohub.org/public/igem/BBa_K1585241/annotation2439786/range2439786/1
type: Range
persistent identity: range2439786
display id: range2439786
version: 1
top level: BBa_K1585241
direction: direction/reverse
start: 1227
end: 1883
orientation: inline

BBa_K1585997
type: SequenceAnnotation
persistent identity: annotation2439787
display id: annotation2439787
version: 1
top level: BBa_K1585241
location: https://synbiohub.org/public/igem/BBa_K1585241/annotation2439787/range2439787/1
component: BBa_K1585997

https://synbiohub.org/public/igem/BBa_K1585241/annotation2439787/range2439787/1
type: Range
persistent identity: range2439787
display id: range2439787
version: 1
top level: BBa_K1585241
direction: direction/reverse
start: 1884
end: 1887
orientation: inline

BBa_K1585212
type: SequenceAnnotation
persistent identity: annotation2439791
display id: annotation2439791
version: 1
top level: BBa_K1585241
location: https://synbiohub.org/public/igem/BBa_K1585241/annotation2439791/range2439791/1
component: BBa_K1585212

https://synbiohub.org/public/igem/BBa_K1585241/annotation2439791/range2439791/1
type: Range
persistent identity: range2439791
display id: range2439791
version: 1
top level: BBa_K1585241
direction: direction/reverse
start: 1888
end: 2463
orientation: inline

BBa_K1585996
type: SequenceAnnotation
persistent identity: annotation2439792
display id: annotation2439792
version: 1
top level: BBa_K1585241
location: https://synbiohub.org/public/igem/BBa_K1585241/annotation2439792/range2439792/1
component: BBa_K1585996

https://synbiohub.org/public/igem/BBa_K1585241/annotation2439792/range2439792/1
type: Range
persistent identity: range2439792
display id: range2439792
version: 1
top level: BBa_K1585241
direction: direction/reverse
start: 2464
end: 2467
orientation: inline

BBa_K1585223
type: SequenceAnnotation
persistent identity: annotation2439805
display id: annotation2439805
version: 1
top level: BBa_K1585241
location: https://synbiohub.org/public/igem/BBa_K1585241/annotation2439805/range2439805/1
component: BBa_K1585223

https://synbiohub.org/public/igem/BBa_K1585241/annotation2439805/range2439805/1
type: Range
persistent identity: range2439805
display id: range2439805
version: 1
top level: BBa_K1585241
direction: direction/reverse
start: 2468
end: 5103
orientation: inline

BBa_K1585210
type: ComponentDefinition
persistent identity: BBa K1585210
display id: BBa_K1585210
version: 1
was generated by: iGEM to SBOL conversion
description: B0034.Mdh2
created: 2015-08-23T11:00:00Z
modified: 2015-09-17T02:19:10Z
mutable provenance: Bacillus methanolicus MGA3
owned by: james
owned by: myers
mutable description: This is the translational unit of methanol dehydrogenase 2 from Bacillus methanolicus MGA3. It is codon optimized for E.coli. The mdh catalyzes the conversion of methanol to formaldehyde.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2002_
m user id: 24474
owner id: 24505
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: RFC10 compatible
star: false
creator: Laura Grabowski
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1585200
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1585200
sequence: https://synbiohub.org/public/igem/BBa_K1585210_sequence/1

BBa_K1585200
type: Component
persistent identity: component2439406
display id: component2439406
version: 1
top level: BBa_K1585210
definition: BBa_K1585200
access: public

BBa_B0034
type: Component
persistent identity: component2439404
display id: component2439404
version: 1
top level: BBa_K1585210
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2439404
display id: annotation2439404
version: 1
top level: BBa_K1585210
location: https://synbiohub.org/public/igem/BBa_K1585210/annotation2439404/range2439404/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1585210/annotation2439404/range2439404/1
type: Range
persistent identity: range2439404
display id: range2439404
version: 1
top level: BBa_K1585210
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1585200
type: SequenceAnnotation
persistent identity: annotation2439406
display id: annotation2439406
version: 1
top level: BBa_K1585210
location: https://synbiohub.org/public/igem/BBa_K1585210/annotation2439406/range2439406/1
component: BBa_K1585200

https://synbiohub.org/public/igem/BBa_K1585210/annotation2439406/range2439406/1
type: Range
persistent identity: range2439406
display id: range2439406
version: 1
top level: BBa_K1585210
direction: direction/reverse
start: 19
end: 1179
orientation: inline

BBa_K1585998
type: ComponentDefinition
persistent identity: BBa K1585998
display id: BBa_K1585998
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter-RBS spacer
created: 2015-08-27T11:00:00Z
modified: 2015-08-28T07:34:54Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: We used this sequence to get the correct spacing between promoter and RBS sequence.
discontinued: false
dominant: false
group u list: _2002_
m user id: 19522
owner id: 19522
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: it resembles the beginning of the RFC10 scar
star: false
creator: Michael Osthege
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1585998_sequence/1

BBa_K1585997
type: ComponentDefinition
persistent identity: BBa K1585997
display id: BBa_K1585997
version: 1
was generated by: iGEM to SBOL conversion
description: RDP X-scar
created: 2015-08-27T11:00:00Z
modified: 2015-08-28T07:39:17Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: This scar is created by RDP-assembly of two X-ends
discontinued: false
dominant: false
group u list: _2002_
m user id: 19522
owner id: 19522
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Michael Osthege
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1585997_sequence/1

BBa_K1585996
type: ComponentDefinition
persistent identity: BBa K1585996
display id: BBa_K1585996
version: 1
was generated by: iGEM to SBOL conversion
description: RDP Z-scar
created: 2015-08-27T11:00:00Z
modified: 2015-08-28T07:41:55Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: This scar is created by RDP-assembly of two Z-ends
discontinued: false
dominant: false
group u list: _2002_
m user id: 19522
owner id: 19522
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Michael Osthege
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K1585996_sequence/1

BBa_K1585223
type: ComponentDefinition
persistent identity: BBa K1585223
display id: BBa_K1585223
version: 1
was generated by: iGEM to SBOL conversion
description: B0034.Xpk.B0015
created: 2015-08-27T11:00:00Z
modified: 2015-08-28T09:26:42Z
mutable provenance: assembled from other parts
owned by: james
owned by: myers
mutable description: protein generator of Xpk (phosphoketolase)
discontinued: false
dominant: false
group u list: _2002_
m user id: 19522
owner id: 19522
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: this is a precursor for another part
star: false
creator: Michael Osthege
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_K1585213
sequence annotation: BBa_K1585213
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1585223_sequence/1

BBa_B0015
type: Component
persistent identity: component2439654
display id: component2439654
version: 1
top level: BBa_K1585223
definition: BBa_B0015
access: public

BBa_K1585213
type: Component
persistent identity: component2439647
display id: component2439647
version: 1
top level: BBa_K1585223
definition: BBa_K1585213
access: public

BBa_K1585213
type: SequenceAnnotation
persistent identity: annotation2439647
display id: annotation2439647
version: 1
top level: BBa_K1585223
location: https://synbiohub.org/public/igem/BBa_K1585223/annotation2439647/range2439647/1
component: BBa_K1585213

https://synbiohub.org/public/igem/BBa_K1585223/annotation2439647/range2439647/1
type: Range
persistent identity: range2439647
display id: range2439647
version: 1
top level: BBa_K1585223
direction: direction/reverse
start: 1
end: 2499
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2439654
display id: annotation2439654
version: 1
top level: BBa_K1585223
location: https://synbiohub.org/public/igem/BBa_K1585223/annotation2439654/range2439654/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1585223/annotation2439654/range2439654/1
type: Range
persistent identity: range2439654
display id: range2439654
version: 1
top level: BBa_K1585223
direction: direction/reverse
start: 2508
end: 2636
orientation: inline

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

BBa_K1585211
type: ComponentDefinition
persistent identity: BBa K1585211
display id: BBa_K1585211
version: 1
was generated by: iGEM to SBOL conversion
description: B0034.HPS
created: 2015-08-24T11:00:00Z
modified: 2015-08-28T08:21:36Z
mutable provenance: Bacillus methanolicus MGA3
owned by: james
owned by: myers
mutable description: This is the translational unit of 3-hexulose-6-phosphate synthase (HPS) from Bacillus methanolicus MGA3. It is codon optimized for E.coli.
discontinued: false
dominant: false
group u list: _2002_
m user id: 19522
owner id: 24505
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: RFC10 compatible
star: false
creator: Laura Grabowski
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1585201
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1585201
sequence: https://synbiohub.org/public/igem/BBa_K1585211_sequence/1

BBa_K1585201
type: Component
persistent identity: component2439453
display id: component2439453
version: 1
top level: BBa_K1585211
definition: BBa_K1585201
access: public

BBa_B0034
type: Component
persistent identity: component2439452
display id: component2439452
version: 1
top level: BBa_K1585211
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2439452
display id: annotation2439452
version: 1
top level: BBa_K1585211
location: https://synbiohub.org/public/igem/BBa_K1585211/annotation2439452/range2439452/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1585211/annotation2439452/range2439452/1
type: Range
persistent identity: range2439452
display id: range2439452
version: 1
top level: BBa_K1585211
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1585201
type: SequenceAnnotation
persistent identity: annotation2439453
display id: annotation2439453
version: 1
top level: BBa_K1585211
location: https://synbiohub.org/public/igem/BBa_K1585211/annotation2439453/range2439453/1
component: BBa_K1585201

https://synbiohub.org/public/igem/BBa_K1585211/annotation2439453/range2439453/1
type: Range
persistent identity: range2439453
display id: range2439453
version: 1
top level: BBa_K1585211
direction: direction/reverse
start: 19
end: 657
orientation: inline

BBa_K1585212
type: ComponentDefinition
persistent identity: BBa K1585212
display id: BBa_K1585212
version: 1
was generated by: iGEM to SBOL conversion
description: B0034.PHI
created: 2015-08-24T11:00:00Z
modified: 2015-08-28T08:24:03Z
mutable provenance: Bacillus methanolicus
owned by: james
owned by: myers
mutable description: This is the translational unit of the 6-phospho-3-hexuloisomerase (PHI) from Bacillus methanolicus MGA3. It is codon optimized for E.coli.
discontinued: false
dominant: false
group u list: _2002_
m user id: 19522
owner id: 24505
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: RFC10 compatible
star: false
creator: Laura Grabowski
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1585202
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1585202
sequence: https://synbiohub.org/public/igem/BBa_K1585212_sequence/1

BBa_K1585202
type: Component
persistent identity: component2439456
display id: component2439456
version: 1
top level: BBa_K1585212
definition: BBa_K1585202
access: public

BBa_B0034
type: Component
persistent identity: component2439455
display id: component2439455
version: 1
top level: BBa_K1585212
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2439455
display id: annotation2439455
version: 1
top level: BBa_K1585212
location: https://synbiohub.org/public/igem/BBa_K1585212/annotation2439455/range2439455/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1585212/annotation2439455/range2439455/1
type: Range
persistent identity: range2439455
display id: range2439455
version: 1
top level: BBa_K1585212
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1585202
type: SequenceAnnotation
persistent identity: annotation2439456
display id: annotation2439456
version: 1
top level: BBa_K1585212
location: https://synbiohub.org/public/igem/BBa_K1585212/annotation2439456/range2439456/1
component: BBa_K1585202

https://synbiohub.org/public/igem/BBa_K1585212/annotation2439456/range2439456/1
type: Range
persistent identity: range2439456
display id: range2439456
version: 1
top level: BBa_K1585212
direction: direction/reverse
start: 19
end: 576
orientation: inline

BBa_K1585200
type: ComponentDefinition
persistent identity: BBa K1585200
display id: BBa_K1585200
version: 1
was generated by: iGEM to SBOL conversion
description: Mdh2
created: 2015-08-23T11:00:00Z
modified: 2015-08-28T08:04:53Z
mutable provenance: Bacillus methanolicus MGA3
owned by: james
owned by: myers
mutable description: Methanol dehydrogenase from Bacillus methanolicus MGA3, codon optimized for E.coli. NADH dependent alcohol dehydrogenase that converts methanol to formaldehyde.
discontinued: false
dominant: false
group u list: _2002_
m user id: 19522
owner id: 24470
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Coding sequence
star: false
creator: Carolin Gr??tering
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Mdh2
sequence: https://synbiohub.org/public/igem/BBa_K1585200_sequence/1

Mdh2
type: SequenceAnnotation
persistent identity: annotation2438209
display id: annotation2438209
version: 1
top level: BBa_K1585200
location: https://synbiohub.org/public/igem/BBa_K1585200/annotation2438209/range2438209/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1585200/annotation2438209/range2438209/1
type: Range
persistent identity: range2438209
display id: range2438209
version: 1
top level: BBa_K1585200
direction: direction/forward
start: 1
end: 1161
orientation: inline

BBa_K1585201
type: ComponentDefinition
persistent identity: BBa K1585201
display id: BBa_K1585201
version: 1
was generated by: iGEM to SBOL conversion
description: HPS
created: 2015-08-24T11:00:00Z
modified: 2015-08-28T08:20:38Z
mutable provenance: Bacillus methanolicus
owned by: james
owned by: myers
mutable description: 3-hexulose-6-phosphate synthase from Hps Bacillus methanolicus MGA3, codon optimized for E.coli
discontinued: false
dominant: false
group u list: _2002_
m user id: 19522
owner id: 24470
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: RFC10 compatible
star: false
creator: Carolin Gr??tering
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: hps
sequence: https://synbiohub.org/public/igem/BBa_K1585201_sequence/1

hps
type: SequenceAnnotation
persistent identity: annotation2474236
display id: annotation2474236
version: 1
top level: BBa_K1585201
location: https://synbiohub.org/public/igem/BBa_K1585201/annotation2474236/range2474236/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1585201/annotation2474236/range2474236/1
type: Range
persistent identity: range2474236
display id: range2474236
version: 1
top level: BBa_K1585201
direction: direction/forward
start: 1
end: 639
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K1585213
type: ComponentDefinition
persistent identity: BBa K1585213
display id: BBa_K1585213
version: 1
was generated by: iGEM to SBOL conversion
description: B0034.XPK
created: 2015-08-24T11:00:00Z
modified: 2015-08-28T08:37:34Z
mutable provenance: Bifidobacterium adolescentis
owned by: james
owned by: myers
mutable description: This is the translational unit for the phosphoketolase (XPK). It derives from Bifidobacterium adolescentis and is codon optimized for E. coli.
discontinued: false
dominant: false
group u list: _2002_
m user id: 19522
owner id: 24505
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: RFC10 compatible
star: false
creator: Laura Grabowski
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1585203
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1585203
sequence: https://synbiohub.org/public/igem/BBa_K1585213_sequence/1

BBa_K1585203
type: Component
persistent identity: component2439488
display id: component2439488
version: 1
top level: BBa_K1585213
definition: BBa_K1585203
access: public

BBa_B0034
type: Component
persistent identity: component2439486
display id: component2439486
version: 1
top level: BBa_K1585213
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2439486
display id: annotation2439486
version: 1
top level: BBa_K1585213
location: https://synbiohub.org/public/igem/BBa_K1585213/annotation2439486/range2439486/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1585213/annotation2439486/range2439486/1
type: Range
persistent identity: range2439486
display id: range2439486
version: 1
top level: BBa_K1585213
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1585203
type: SequenceAnnotation
persistent identity: annotation2439488
display id: annotation2439488
version: 1
top level: BBa_K1585213
location: https://synbiohub.org/public/igem/BBa_K1585213/annotation2439488/range2439488/1
component: BBa_K1585203

https://synbiohub.org/public/igem/BBa_K1585213/annotation2439488/range2439488/1
type: Range
persistent identity: range2439488
display id: range2439488
version: 1
top level: BBa_K1585213
direction: direction/reverse
start: 19
end: 2499
orientation: inline

BBa_K1585202
type: ComponentDefinition
persistent identity: BBa K1585202
display id: BBa_K1585202
version: 1
was generated by: iGEM to SBOL conversion
description: PHI
created: 2015-08-24T11:00:00Z
modified: 2015-08-28T08:23:36Z
mutable provenance: Bacillus methanolicus
owned by: james
owned by: myers
mutable description: 6-phospho-3-hexuloisomerase from Bacillus methanolicus MGA3, codon optimized for E.coli, converts D-arabino-hex-3-ulose 6-phosphate to D-fructose 6-phosphate
discontinued: false
dominant: false
group u list: _2002_
m user id: 19522
owner id: 24470
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: codon optimized
star: false
creator: Carolin Gr??tering
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: phi
sequence: https://synbiohub.org/public/igem/BBa_K1585202_sequence/1

phi
type: SequenceAnnotation
persistent identity: annotation2474281
display id: annotation2474281
version: 1
top level: BBa_K1585202
location: https://synbiohub.org/public/igem/BBa_K1585202/annotation2474281/range2474281/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1585202/annotation2474281/range2474281/1
type: Range
persistent identity: range2474281
display id: range2474281
version: 1
top level: BBa_K1585202
direction: direction/forward
start: 1
end: 558
orientation: inline

BBa_K1585203
type: ComponentDefinition
persistent identity: BBa K1585203
display id: BBa_K1585203
version: 1
was generated by: iGEM to SBOL conversion
description: XPK
created: 2015-08-24T11:00:00Z
modified: 2015-08-28T08:33:31Z
mutable provenance: Bifidobacterium adolescentis
owned by: james
owned by: myers
mutable description: phosphoketolase from Bifidobacterium adolescentis, codon optimized for E.coli, converts Xu5p and pi to actp and g3p
discontinued: false
dominant: false
group u list: _2002_
m user id: 19522
owner id: 24470
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: codon optimized for E.coli
star: false
creator: Carolin Gr??tering
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: phosphoketolase
sequence: https://synbiohub.org/public/igem/BBa_K1585203_sequence/1

phosphoketolase
type: SequenceAnnotation
persistent identity: annotation2439475
display id: annotation2439475
version: 1
top level: BBa_K1585203
location: https://synbiohub.org/public/igem/BBa_K1585203/annotation2439475/range2439475/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1585203/annotation2439475/range2439475/1
type: Range
persistent identity: range2439475
display id: range2439475
version: 1
top level: BBa_K1585203
direction: direction/forward
start: 1
end: 2481
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1585241_sequence/1
type: Sequence
persistent identity: BBa K1585241 sequence
display id: BBa_K1585241_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagctactgatgaaagaggagaaatactagatgaccaacacccagagcgcgttttttatgccgagcgtgaacctgtttggcgcgggcagcgtgaacgaagtgggcacccgcctggcggatctgggcgtgaaaaaagcgctgctggtgaccgatgcgggcctgcatggcctgggcctgagcgaaaaaattagcagcattattcgcgcggcgggcgtggaagtgagcatttttccgaaagcggaaccgaacccgaccgataaaaacgtggcggaaggcctggaagcgtataacgcggaaaactgcgatagcattgtgaccctgggcggcggcagcagccatgatgcgggcaaagcgattgcgctggtggcggcgaacggcggcaaaattcatgattatgaaggcgtggatgtgagcaaagaaccgatggtgccgctgattgcgattaacaccaccgcgggcaccggcagcgaactgaccaaatttaccattattaccgataccgaacgcaaagtgaaaatggcgattgtggataaacatgtgaccccgaccctgagcattaacgatccggaactgatggtgggcatgccgccgagcctgaccgcggcgaccggtctggatgcgctgacccacgcgatcgaagcgtacgttagcacaggcgccaccccgatcaccgacgcgctggcgattcaggcgatcaaaatcattagcaaatatctgccgcgtgcggtggccaacggtaaagatatcgaagctcgcgaacagatggcgttcgcccagagcctggcgggcatggcgtttaacaacgcgggcctgggctatgtgcatgcgatcgcgcatcaactgggcggcttttataactttccgcatggcgtgtgcaacgcggtgctgctgccgtatgtgtgccgctttaacctgattagcaaagtggaacgctatgcggaaattgcggcgtttctgggtgaaaacgttgatggtctgagcacctacgatgcggccgaaaaagcgatcaaagcgatcgaacgtatggcgaaagacctgaacattccgaaaggctttaaagaactgggcgcgaaagaagaagatattgaaaccctggcgaaaaacgcgatgaaagatgcgtgcgcgctgaccaacccgcgcaaaccgaaactggaagaagtgattcagattattaaaaacgcgatgtaataacggcaaagaggagaaatactagatggaactgcaactggcgctggatctggtgaacattgaagaagcgaaacaggtggtggcggaagtgcaggaatatgtggatattgtggaaattggcaccccggtgattaaaatttggggcctgcaagcggtgaaagcggtgaaagatgcgtttccgcatctgcaagtgctggcggatatgaaaaccatggatgcggcggcgtatgaagtggcgaaagcggcggaacatggcgcggatattgtgaccattctggcggcggcggaagatgtgagcattaaaggcgcggtggaagaagcgaaaaaactgggcaaaaaaattctggtggatatgattgcggtgaaaaacctggaagaacgcgcgaaacaggtggatgaaatgggcgtggattatatttgcgtgcatgcgggttatgatctgcaagccgttggcaagaacccgctggatgatctgaaacgcattaaggcggtggtgaaaaacgcgaaaaccgcgattgcgggcggcattaaactggaaaccctgccggaagtgattaaagcggaaccggatctggtgattgtgggcggcggcattgcgaaccagaccgataaaaaagcggcggcggaaaaaattaacaaactggtgaaacagggcctgtaataagatgaaagaggagaaatactagatgctgaccaccgaatttctggcggaaattgtgaaagaactgaacagcagcgtgaaccagattgcggatgaagaagcggaagcgctggtgaacgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1585210_sequence/1
type: Sequence
persistent identity: BBa K1585210 sequence
display id: BBa_K1585210_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaccaacacccagagcgcgttttttatgccgagcgtgaacctgtttggcgcgggcagcgtgaacgaagtgggcacccgcctggcggatctgggcgtgaaaaaagcgctgctggtgaccgatgcgggcctgcatggcctgggcctgagcgaaaaaattagcagcattattcgcgcggcgggcgtggaagtgagcatttttccgaaagcggaaccgaacccgaccgataaaaacgtggcggaaggcctggaagcgtataacgcggaaaactgcgatagcattgtgaccctgggcggcggcagcagccatgatgcgggcaaagcgattgcgctggtggcggcgaacggcggcaaaattcatgattatgaaggcgtggatgtgagcaaagaaccgatggtgccgctgattgcgattaacaccaccgcgggcaccggcagcgaactgaccaaatttaccattattaccgataccgaacgcaaagtgaaaatggcgattgtggataaacatgtgaccccgaccctgagcattaacgatccggaactgatggtgggcatgccgccgagcctgaccgcggcgaccggtctggatgcgctgacccacgcgatcgaagcgtacgttagcacaggcgccaccccgatcaccgacgcgctggcgattcaggcgatcaaaatcattagcaaatatctgccgcgtgcggtggccaacggtaaagatatcgaagctcgcgaacagatggcgttcgcccagagcctggcgggcatggcgtttaacaacgcgggcctgggctatgtgcatgcgatcgcgcatcaactgggcggcttttataactttccgcatggcgtgtgcaacgcggtgctgctgccgtatgtgtgccgctttaacctgattagcaaagtggaacgctatgcggaaattgcggcgtttctgggtgaaaacgttgatggtctgagcacctacgatgcggccgaaaaagcgatcaaagcgatcgaacgtatggcgaaagacctgaacattccgaaaggctttaaagaactgggcgcgaaagaagaagatattgaaaccctggcgaaaaacgcgatgaaagatgcgtgcgcgctgaccaacccgcgcaaaccgaaactggaagaagtgattcagattattaaaaacgcgatgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1585998_sequence/1
type: Sequence
persistent identity: BBa K1585998 sequence
display id: BBa_K1585998_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1585997_sequence/1
type: Sequence
persistent identity: BBa K1585997 sequence
display id: BBa_K1585997_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1585996_sequence/1
type: Sequence
persistent identity: BBa K1585996 sequence
display id: BBa_K1585996_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1585223_sequence/1
type: Sequence
persistent identity: BBa K1585223 sequence
display id: BBa_K1585223_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaccagcccggtgattggcaccccgtggaaaaaactgaacgcgccggtgagcgaagaagcgattgaaggcgtggataaatattggcgcgcggcgaactatctgagcattggccagatttatctgcgtagcaacccgctgatgaaagaaccgtttacccgcgaagatgtgaaacatcgcctggtgggccattggggcaccaccccgggcctgaactttctgattggccatattaaccgcctgattgcggatcatcagcagaacaccgtgattattatgggcccgggccatggcggcccggcgggcaccgcgcagagctatctggatggcacctataccgaatattttccgaacattaccaaagatgaagcgggcctgcaaaaattttttcgccagtttagctatccgggcggcattccgagccattatgcgccggaaaccccgggcagcattcatgaaggcggcgaactgggctatgcgctgagccatgcgtatggcgcggtgatgaacaacccgagcctgtttgttccggcgattgtgggcgatggcgaagcggaaaccggcccgctggcgaccggctggcagagcaacaaactgattaacccgcgcaccgatggcattgtgctgccgattctgcatctgaacggctataaaattgcgaacccgaccattctgagccgcattagcgatgaagaactgcatgaattttttcatggcatgggctatgaaccgtatgaatttgtggcgggctttgataacgaagatcatctgagcattcatcgccgctttgcggaactgtttgaaaccgtgtttgatgaaatttgcgatattaaagcggcggcgcagaccgatgatatgacccgcccgttttatccgatgattatttttcgcaccccgaaaggctggacctgcccgaaatttattgatggcaaaaaaaccgaaggtagctggcgcagccatcaggtgccgctggcgagcgcgcgcgataccgaagcgcattttgaagtgctgaaaaactggctggaaagctataaaccggaagaactgtttgatgaaaacggcgcggtgaaaccggaagtgaccgcgtttatgccgaccggcgaactgcgtattggcgaaaacccgaacgcgaacggtggtcgtattcgcgaagaactgaaactgccgaaactggaagattatgaagttaaagaagtggccgagtatggtcatggttggggtcaactggaagcgacccgccgcctgggcgtttatacccgcgatattattaaaaacaacccggattctttccgcatcttcggtccggatgaaaccgcctctaaccgcctgcaagcggcctatgatgtgaccaacaaacagtgggatgcgggttatctgtctgcccaggttgatgaacatatggcggtgaccggtcaggttaccgaacaactgagcgaacatcagatggaaggctttctggaaggctatctgctgaccggccgccatggcatctggtctagctatgaaagcttcgtgcatgttatcgattctatgctgaaccagcatgccaaatggctggaagcgaccgttcgcgaaattccgtggcgcaaaccgatctctagcatgaacctgctggttagcagccatgtgtggcgccaggatcataacggctttagccatcaggacccgggcgttaccagcgtgctgctgaacaaatgcttcaataacgaccatgtgatcggcatttattttccggtggatagcaacatgctgctggccgttgcggaaaaatgctataaaagcaccaacaaaattaacgccattattgcgggcaaacaaccggccgcgacctggctgaccctggacgaagcccgcgcggaactggaaaaaggcgccgcggaatggaaatgggcgagcaacgtgaaaagcaacgatgaagcgcagattgtgctggcggcgaccggcgatgtgccgacccaggaaattatggcggcggcggataa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23119_sequence/1
type: Sequence
persistent identity: BBa J23119 sequence
display id: BBa_J23119_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1585211_sequence/1
type: Sequence
persistent identity: BBa K1585211 sequence
display id: BBa_K1585211_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggaactgcaactggcgctggatctggtgaacattgaagaagcgaaacaggtggtggcggaagtgcaggaatatgtggatattgtggaaattggcaccccggtgattaaaatttggggcctgcaagcggtgaaagcggtgaaagatgcgtttccgcatctgcaagtgctggcggatatgaaaaccatggatgcggcggcgtatgaagtggcgaaagcggcggaacatggcgcggatattgtgaccattctggcggcggcggaagatgtgagcattaaaggcgcggtggaagaagcgaaaaaactgggcaaaaaaattctggtggatatgattgcggtgaaaaacctggaagaacgcgcgaaacaggtggatgaaatgggcgtggattatatttgcgtgcatgcgggttatgatctgcaagccgttggcaagaacccgctggatgatctgaaacgcattaaggcggtggtgaaaaacgcgaaaaccgcgattgcgggcggcattaaactggaaaccctgccggaagtgattaaagcggaaccggatctggtgattgtgggcggcggcattgcgaaccagaccgataaaaaagcggcggcggaaaaaattaacaaactggtgaaacagggcctgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1585212_sequence/1
type: Sequence
persistent identity: BBa K1585212 sequence
display id: BBa_K1585212_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgctgaccaccgaatttctggcggaaattgtgaaagaactgaacagcagcgtgaaccagattgcggatgaagaagcggaagcgctggtgaacggcattctgcaaagcaaaaaagtgtttgtggcgggcgcgggccgcagcggctttatggcgaaaagctttgcgatgcgtatgatgcatatgggcattgatgcgtatgtggtgggcgaaaccgtgaccccgaactatgaaaaagaagatattctgattattggcagcggcagcggcgaaaccaaaagcctggtgagcatggcgcagaaagcgaaaagcattggcggcaccattgcggcggtgaccattaacccggaaagcaccattggccaactggcggatattgtgattaaaatgccgggcagcccgaaagataaaagcgaagcgcgcgaaaccattcagccgatgggcagcctgtttgaacagaccctgctgctgttttatgatgcggtgattctgcgctttatggaaaaaaaaggcctggataccaaaaccatgtatggccgccatgcgaacctggaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1585200_sequence/1
type: Sequence
persistent identity: BBa K1585200 sequence
display id: BBa_K1585200_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccaacacccagagcgcgttttttatgccgagcgtgaacctgtttggcgcgggcagcgtgaacgaagtgggcacccgcctggcggatctgggcgtgaaaaaagcgctgctggtgaccgatgcgggcctgcatggcctgggcctgagcgaaaaaattagcagcattattcgcgcggcgggcgtggaagtgagcatttttccgaaagcggaaccgaacccgaccgataaaaacgtggcggaaggcctggaagcgtataacgcggaaaactgcgatagcattgtgaccctgggcggcggcagcagccatgatgcgggcaaagcgattgcgctggtggcggcgaacggcggcaaaattcatgattatgaaggcgtggatgtgagcaaagaaccgatggtgccgctgattgcgattaacaccaccgcgggcaccggcagcgaactgaccaaatttaccattattaccgataccgaacgcaaagtgaaaatggcgattgtggataaacatgtgaccccgaccctgagcattaacgatccggaactgatggtgggcatgccgccgagcctgaccgcggcgaccggtctggatgcgctgacccacgcgatcgaagcgtacgttagcacaggcgccaccccgatcaccgacgcgctggcgattcaggcgatcaaaatcattagcaaatatctgccgcgtgcggtggccaacggtaaagatatcgaagctcgcgaacagatggcgttcgcccagagcctggcgggcatggcgtttaacaacgcgggcctgggctatgtgcatgcgatcgcgcatcaactgggcggcttttataactttccgcatggcgtgtgcaacgcggtgctgctgccgtatgtgtgccgctttaacctgattagcaaagtggaacgctatgcggaaattgcggcgtttctgggtgaaaacgttgatggtctgagcacctacgatgcggccgaaaaagcgatcaaagcgatcgaacgtatggcgaaagacctgaacattccgaaaggctttaaagaactgggcgcgaaagaagaagatattgaaaccctggcgaaaaacgcgatgaaagatgcgtgcgcgctgaccaacccgcgcaaaccgaaactggaagaagtgattcagattattaaaaacgcgatgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1585201_sequence/1
type: Sequence
persistent identity: BBa K1585201 sequence
display id: BBa_K1585201_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggaactgcaactggcgctggatctggtgaacattgaagaagcgaaacaggtggtggcggaagtgcaggaatatgtggatattgtggaaattggcaccccggtgattaaaatttggggcctgcaagcggtgaaagcggtgaaagatgcgtttccgcatctgcaagtgctggcggatatgaaaaccatggatgcggcggcgtatgaagtggcgaaagcggcggaacatggcgcggatattgtgaccattctggcggcggcggaagatgtgagcattaaaggcgcggtggaagaagcgaaaaaactgggcaaaaaaattctggtggatatgattgcggtgaaaaacctggaagaacgcgcgaaacaggtggatgaaatgggcgtggattatatttgcgtgcatgcgggttatgatctgcaagccgttggcaagaacccgctggatgatctgaaacgcattaaggcggtggtgaaaaacgcgaaaaccgcgattgcgggcggcattaaactggaaaccctgccggaagtgattaaagcggaaccggatctggtgattgtgggcggcggcattgcgaaccagaccgataaaaaagcggcggcggaaaaaattaacaaactggtgaaacagggcctgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1585213_sequence/1
type: Sequence
persistent identity: BBa K1585213 sequence
display id: BBa_K1585213_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaccagcccggtgattggcaccccgtggaaaaaactgaacgcgccggtgagcgaagaagcgattgaaggcgtggataaatattggcgcgcggcgaactatctgagcattggccagatttatctgcgtagcaacccgctgatgaaagaaccgtttacccgcgaagatgtgaaacatcgcctggtgggccattggggcaccaccccgggcctgaactttctgattggccatattaaccgcctgattgcggatcatcagcagaacaccgtgattattatgggcccgggccatggcggcccggcgggcaccgcgcagagctatctggatggcacctataccgaatattttccgaacattaccaaagatgaagcgggcctgcaaaaattttttcgccagtttagctatccgggcggcattccgagccattatgcgccggaaaccccgggcagcattcatgaaggcggcgaactgggctatgcgctgagccatgcgtatggcgcggtgatgaacaacccgagcctgtttgttccggcgattgtgggcgatggcgaagcggaaaccggcccgctggcgaccggctggcagagcaacaaactgattaacccgcgcaccgatggcattgtgctgccgattctgcatctgaacggctataaaattgcgaacccgaccattctgagccgcattagcgatgaagaactgcatgaattttttcatggcatgggctatgaaccgtatgaatttgtggcgggctttgataacgaagatcatctgagcattcatcgccgctttgcggaactgtttgaaaccgtgtttgatgaaatttgcgatattaaagcggcggcgcagaccgatgatatgacccgcccgttttatccgatgattatttttcgcaccccgaaaggctggacctgcccgaaatttattgatggcaaaaaaaccgaaggtagctggcgcagccatcaggtgccgctggcgagcgcgcgcgataccgaagcgcattttgaagtgctgaaaaactggctggaaagctataaaccggaagaactgtttgatgaaaacggcgcggtgaaaccggaagtgaccgcgtttatgccgaccggcgaactgcgtattggcgaaaacccgaacgcgaacggtggtcgtattcgcgaagaactgaaactgccgaaactggaagattatgaagttaaagaagtggccgagtatggtcatggttggggtcaactggaagcgacccgccgcctgggcgtttatacccgcgatattattaaaaacaacccggattctttccgcatcttcggtccggatgaaaccgcctctaaccgcctgcaagcggcctatgatgtgaccaacaaacagtgggatgcgggttatctgtctgcccaggttgatgaacatatggcggtgaccggtcaggttaccgaacaactgagcgaacatcagatggaaggctttctggaaggctatctgctgaccggccgccatggcatctggtctagctatgaaagcttcgtgcatgttatcgattctatgctgaaccagcatgccaaatggctggaagcgaccgttcgcgaaattccgtggcgcaaaccgatctctagcatgaacctgctggttagcagccatgtgtggcgccaggatcataacggctttagccatcaggacccgggcgttaccagcgtgctgctgaacaaatgcttcaataacgaccatgtgatcggcatttattttccggtggatagcaacatgctgctggccgttgcggaaaaatgctataaaagcaccaacaaaattaacgccattattgcgggcaaacaaccggccgcgacctggctgaccctggacgaagcccgcgcggaactggaaaaaggcgccgcggaatggaaatgggcgagcaacgtgaaaagcaacgatgaagcgcagattgtgctggcggcgaccggcgatgtgccgacccaggaaattatggcggcggcggataa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1585202_sequence/1
type: Sequence
persistent identity: BBa K1585202 sequence
display id: BBa_K1585202_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgctgaccaccgaatttctggcggaaattgtgaaagaactgaacagcagcgtgaaccagattgcggatgaagaagcggaagcgctggtgaacggcattctgcaaagcaaaaaagtgtttgtggcgggcgcgggccgcagcggctttatggcgaaaagctttgcgatgcgtatgatgcatatgggcattgatgcgtatgtggtgggcgaaaccgtgaccccgaactatgaaaaagaagatattctgattattggcagcggcagcggcgaaaccaaaagcctggtgagcatggcgcagaaagcgaaaagcattggcggcaccattgcggcggtgaccattaacccggaaagcaccattggccaactggcggatattgtgattaaaatgccgggcagcccgaaagataaaagcgaagcgcgcgaaaccattcagccgatgggcagcctgtttgaacagaccctgctgctgttttatgatgcggtgattctgcgctttatggaaaaaaaaggcctggataccaaaaccatgtatggccgccatgcgaacctggaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1585203_sequence/1
type: Sequence
persistent identity: BBa K1585203 sequence
display id: BBa_K1585203_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccagcccggtgattggcaccccgtggaaaaaactgaacgcgccggtgagcgaagaagcgattgaaggcgtggataaatattggcgcgcggcgaactatctgagcattggccagatttatctgcgtagcaacccgctgatgaaagaaccgtttacccgcgaagatgtgaaacatcgcctggtgggccattggggcaccaccccgggcctgaactttctgattggccatattaaccgcctgattgcggatcatcagcagaacaccgtgattattatgggcccgggccatggcggcccggcgggcaccgcgcagagctatctggatggcacctataccgaatattttccgaacattaccaaagatgaagcgggcctgcaaaaattttttcgccagtttagctatccgggcggcattccgagccattatgcgccggaaaccccgggcagcattcatgaaggcggcgaactgggctatgcgctgagccatgcgtatggcgcggtgatgaacaacccgagcctgtttgttccggcgattgtgggcgatggcgaagcggaaaccggcccgctggcgaccggctggcagagcaacaaactgattaacccgcgcaccgatggcattgtgctgccgattctgcatctgaacggctataaaattgcgaacccgaccattctgagccgcattagcgatgaagaactgcatgaattttttcatggcatgggctatgaaccgtatgaatttgtggcgggctttgataacgaagatcatctgagcattcatcgccgctttgcggaactgtttgaaaccgtgtttgatgaaatttgcgatattaaagcggcggcgcagaccgatgatatgacccgcccgttttatccgatgattatttttcgcaccccgaaaggctggacctgcccgaaatttattgatggcaaaaaaaccgaaggtagctggcgcagccatcaggtgccgctggcgagcgcgcgcgataccgaagcgcattttgaagtgctgaaaaactggctggaaagctataaaccggaagaactgtttgatgaaaacggcgcggtgaaaccggaagtgaccgcgtttatgccgaccggcgaactgcgtattggcgaaaacccgaacgcgaacggtggtcgtattcgcgaagaactgaaactgccgaaactggaagattatgaagttaaagaagtggccgagtatggtcatggttggggtcaactggaagcgacccgccgcctgggcgtttatacccgcgatattattaaaaacaacccggattctttccgcatcttcggtccggatgaaaccgcctctaaccgcctgcaagcggcctatgatgtgaccaacaaacagtgggatgcgggttatctgtctgcccaggttgatgaacatatggcggtgaccggtcaggttaccgaacaactgagcgaacatcagatggaaggctttctggaaggctatctgctgaccggccgccatggcatctggtctagctatgaaagcttcgtgcatgttatcgattctatgctgaaccagcatgccaaatggctggaagcgaccgttcgcgaaattccgtggcgcaaaccgatctctagcatgaacctgctggttagcagccatgtgtggcgccaggatcataacggctttagccatcaggacccgggcgttaccagcgtgctgctgaacaaatgcttcaataacgaccatgtgatcggcatttattttccggtggatagcaacatgctgctggccgttgcggaaaaatgctataaaagcaccaacaaaattaacgccattattgcgggcaaacaaccggccgcgacctggctgaccctggacgaagcccgcgcggaactggaaaaaggcgccgcggaatggaaatgggcgagcaacgtgaaaagcaacgatgaagcgcagattgtgctggcggcgaccggcgatgtgccgacccaggaaattatggcggcggcggataaactggatgcgatgggcat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z