polycistronic expression part for methanol assimilation genes
the part is a mix of BioBrick and RDP assembly
This is the translational unit of methanol dehydrogenase 2 from Bacillus methanolicus MGA3. It is codon optimized for E.coli. The mdh catalyzes the conversion of methanol to formaldehyde.
We used this sequence to get the correct spacing between promoter and RBS sequence.
it resembles the beginning of the RFC10 scar
This scar is created by RDP-assembly of two X-ends
This scar is created by RDP-assembly of two Z-ends
protein generator of Xpk (phosphoketolase)
Overlap extension of synthetic oligonucleotides
This is the translational unit of 3-hexulose-6-phosphate synthase (HPS) from Bacillus methanolicus MGA3. It is codon optimized for E.coli.
This is the translational unit of the 6-phospho-3-hexuloisomerase (PHI) from Bacillus methanolicus MGA3. It is codon optimized for E.coli.
Methanol dehydrogenase from Bacillus methanolicus MGA3, codon optimized for E.coli. NADH dependent alcohol dehydrogenase that converts methanol to formaldehyde.
3-hexulose-6-phosphate synthase from Hps Bacillus methanolicus MGA3, codon optimized for E.coli
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Double terminator consisting of BBa_B0010 and BBa_B0012
This is the translational unit for the phosphoketolase (XPK). It derives from Bifidobacterium adolescentis and is codon optimized for E. coli.
6-phospho-3-hexuloisomerase from Bacillus methanolicus MGA3, codon optimized for E.coli, converts D-arabino-hex-3-ulose 6-phosphate to D-fructose 6-phosphate
phosphoketolase from Bifidobacterium adolescentis, codon optimized for E.coli, converts Xu5p and pi to actp and g3p
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1