LOCUS BBa_K1585321 4996 bp DNA linear 08-May-2024 DEFINITION glgCAB for polycistronic expression of glycogen synthesis genes in E.coli ACCESSION BBa_K1585321 VERSION BBa_K1585321.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 1024..1026 /label=G336D misc_feature 589..594 /label=EcoRI site removed misc_feature 1086..1091 /label=EcoRI site removed CDS 19..1317 /label=BBa_K118016 misc_feature 1..1317 /label=BBa_K1585312 misc_feature 1330..1333 /label=conserved RBS 1326..1337 /label=BBa_B0034 CDS 1344..2780 /label=glgA misc_feature 1505..1505 /label=Replaced G with C to remove AviII site misc_feature 1748..1748 /label=Replaced G with C to remove AvrII site misc_feature 1916..1916 /label=Replaced C with T to remove EcoRI site misc_feature 2312..2312 /label=Replaced G with A to remove PvuII site misc_feature 2348..2348 /label=Replaced G with A to remove PstI site misc_feature 2540..2540 /label=Replaced T with C to remove AgeI site CDS 1344..2780 /label=BBa_K1585300 misc_feature 1326..2780 /label=BBa_K1585310 misc_feature 2793..2796 /label=conserved RBS 2789..2800 /label=BBa_B0034 CDS 2807..4996 /label=BBa_K1585301 misc_feature 2789..4996 /label=BBa_K1585311 ORIGIN 1 aaagaggaga aatactagat ggttagttta gagaagaacg atcacttaat gttggcgcgc 61 cagctgccat tgaaatctgt tgccctgata ctggcgggag gacgtggtac ccgcctgaag 121 gatttaacca ataagcgagc aaaaccggcc gtacacttcg gcggtaagtt ccgcattatc 181 gactttgcgc tgtctaactg catcaactcc gggatccgtc gtatgggcgt gatcacccag 241 taccagtccc acactctggt gcagcacatt cagcgcggct ggtcattctt caatgaagaa 301 atgaacgagt ttgtcgatct gctgccagca cagcagagaa tgaaagggga aaactggtat 361 cgcggcaccg cagatgcggt cacccaaaac ctcgacatta tccgccgtta taaagcggaa 421 tacgtggtga tcctggcggg cgaccatatc tacaagcaag actactcgcg tatgcttatc 481 gatcacgtcg aaaaaggcgc acgttgcacc gttgcttgta tgccagtacc gattgaagaa 541 gcctccgcat ttggcgttat ggcggttgat gagaacgata aaattatcga atttgttgaa 601 aaacctgcta acccgccgtc aatgccgaac gatccgagca aatctctggc gagtatgggt 661 atctacgtct ttgacgccga ctatctgtat gaactgctgg aagaagacga tcgcgatgag 721 aactccagcc acgactttgg caaagatttg attcccaaga tcaccgaagc cggtctggcc 781 tatgcgcacc cgttcccgct ctcttgcgta caatccgacc cggatgccga gccgtactgg 841 cgcgatgtgg gtacgctgga agcttactgg aaagcgaacc tcgatctggc ctctgtggtg 901 ccggaactgg atatgtacga tcgcaattgg ccaattcgca cctacaatga atcattaccg 961 ccagcgaaat tcgtgcagga tcgctccggt agccacggga tgacccttaa ctcactggtt 1021 tccgacggtt gtgtgatctc cggttcggtg gtggtgcagt ccgttctgtt ctcgcgcgtt 1081 cgcgtgaact cattctgcaa cattgattcc gccgtattgt taccggaagt atgggtaggt 1141 cgctcgtgcc gtctgcgccg ctgcgtcatc gatcgtgctt gtgttattcc ggaaggcatg 1201 gtgattggtg aaaacgcaga ggaagatgca cgtcgtttct atcgttcaga agaaggcatc 1261 gtgctggtaa cgcgcgaaat gctacggaag ttagggcata aacaggagcg ataataatac 1321 tagagaaaga ggagaaatac tagatgcagg ttttacatgt atgttcagag atgttcccgc 1381 tgcttaaaac cggcggtctg gctgatgtta ttggggcatt acccgcagca caaatcgcag 1441 acggcgttga cgctcgcgta ctgttgcctg catttcccga tattcgccgt ggcgtgaccg 1501 atgcccaggt agtatcccgt cgtgatacct tcgccggaca tatcacgctg ttgttcggtc 1561 attacaacgg ggttggcatt tacctgattg acgcgccgca tctctatgat cgtccgggaa 1621 gcccgtatca cgataccaac ttatttgcct ataccgacaa cgtattgcgt tttgcgctgc 1681 tggggtgggt tggggcagaa atggccagcg ggcttgaccc attctggcgt cctgatgtgg 1741 tgcatgccca cgactggcat gcaggccttg cgcctgcgta tctggcggcg cgcgggcgtc 1801 cggcgaagtc ggtgtttact gtgcacaacc tggcctatca aggcatgttt tatgcacatc 1861 acatgaatga catccaattg ccatggtcat tctttaatat tcatgggctg gaatttaacg 1921 gacaaatctc tttcctgaag gccggtctgt actatgccga tcacattacg gcggtcagtc 1981 caacctacgc tcgcgagatc accgaaccgc agtttgccta cggtatggaa ggtctgttgc 2041 aacagcgtca ccgtgaaggg cgtctttccg gcgtactgaa cggcgtggac gagaaaatct 2101 ggagtccaga gacggactta ctgttggcct cgcgttacac ccgcgatacg ttggaagata 2161 aagcggaaaa taagcgccag ttacaaatcg caatggggct taaggttgac gataaagtgc 2221 cgctttttgc agtggtgagc cgtctgacca gccagaaagg tctcgacctg gtgctggaag 2281 ccttaccggg tcttctggag cagggcgggc aactggcgct actcggcgcg ggcgatccgg 2341 tgctgcaaga aggtttcctt gcggcggcag cggaataccc cggtcaggtg ggcgttcaga 2401 ttggctatca cgaagcattt tcgcatcgca ttatgggcgg cgcggacgtc attctggtgc 2461 ccagccgttt tgaaccgtgc ggcttaacgc aactttatgg attgaagtac ggtacgctgc 2521 cgttagtgcg gcgcaccggc gggcttgctg atacggtttc tgactgttct cttgagaacc 2581 ttgcagatgg cgtcgccagt gggtttgtct ttgaagatag taatgcctgg tcgctgttac 2641 gggctattcg acgtgctttt gtactgtggt cccgtccttc actgtggcgg tttgtgcaac 2701 gtcaggctat ggcaatggat tttagctggc aggtcgcggc gaagtcgtac cgtgagcttt 2761 actatcgctt gaaataataa tactagagaa agaggagaaa tactagatgt ccgatcgtat 2821 cgatagagac gtgattaacg cgctaattgc aggccatttt gccgatcctt tttccgtact 2881 gggaatgcat aaaaccaccg cgggactgga agtccgtgcc cttttacccg acgctaccga 2941 tgtgtgggtg attgaaccga aaaccgggcg caaactcgca aaactggagt gtctcgactc 3001 acggggattc tttagcggcg tcattccgcg acgtaagaat tttttccgct atcagttggc 3061 tgttgtctgg catggtcagc aaaacctgat tgatgatcct taccgttttg gtccgctaat 3121 ccaggaaatg gatgcctggc tattatctga aggtactcac ctgcgcccgt atgaaacctt 3181 aggcgcgcat gcagatacta tggatggcgt cacaggtacg cgtttctctg tctgggctcc 3241 aaacgcccgt cgggtctcgg tggttgggca attcaactac tgggacggtc gccgtcaccc 3301 gatgcgcctg cgtaaagaga gcggcatctg ggaactgttt atccctgggg cgcataacgg 3361 tcagctctat aaatacgaga tgattgatgc caatggcaac ttgcgtctga agtccgaccc 3421 ttatgccttt gaagcgcaaa tgcgcccgga aaccgcgtct cttatttgcg ggctgccgga 3481 aaaggttgta cagactgaag aacgcaaaaa agcgaatcag tttgatgcgc caatctctat 3541 ttatgaagtt cacctgggtt cctggcgtcg ccacaccgac aacaatttct ggttgagcta 3601 ccgcgagctg gccgatcaac tggtgcctta tgctaaatgg atgggcttta cccacctcga 3661 actactgccc attaacgagc atcccttcga tggcagttgg ggttatcagc caaccggcct 3721 gtatgcgcca acccgccgtt ttggtactcg cgacgacttc cgttatttca ttgatgccgc 3781 acacgcggct ggtctgaacg tgattctcga ctgggtgcca ggccacttcc cgactgatga 3841 ctttgcgctt gccgaatttg atggcacgaa cttgtatgaa cacagcgatc cgcgtgaagg 3901 ctatcatcag gactggaaca cgctgatcta caactatggt cgccgtgaag tcagtaactt 3961 cctcgtcggt aacgcgcttt actggattga acgttttggt attgatgcgc tgcgcgtcga 4021 tgcggtggcg tcaatgattt atcgcgacta cagccgtaaa gagggggagt ggattccgaa 4081 cgaatttggc gggcgcgaga atcttgaagc gattgaattt ttgcgtaata ccaaccgtat 4141 tcttggtgag caggtttccg gtgcggtgac aatggctgag gagtctaccg atttccctgg 4201 cgtttctcgt ccgcaggata tgggcggtct gggcttctgg tacaagtgga acctcggctg 4261 gatgcatgac accctggact acatgaagct cgacccggtt tatcgtcagt atcatcacga 4321 taaactgacc ttcgggattc tctacaacta cactgaaaac ttcgtcctgc cgttgtcgca 4381 tgatgaagtg gtccacggta aaaaatcgat tctcgaccgc atgccgggcg acgcatggca 4441 gaaattcgcg aacctgcgcg cctactatgg ctggatgtgg gcattcccgg gcaagaaact 4501 actgttcatg ggtaacgaat ttgcccaggg ccgcgagtgg aaccatgacg ccagcctcga 4561 ctggcatctg ttggaaggcg gcgataactg gcaccacggt gtccagcgtc tggtgcgcga 4621 tctgaacctc acctaccgcc accataaagc aatgcatgaa ctggattttg acccgtacgg 4681 ctttgaatgg ctggtggtgg atgacaaaga acgctcggtg ctgatctttg tgcgtcgcga 4741 taaagagggt aacgaaatca tcgttgccag taactttacg ccggtaccgc gtcatgatta 4801 tcgcttcggc ataaaccagc cgggcaaatg gcgtgaaatc ctcaataccg attccatgca 4861 ctatcacggc agtaatgcag gcaatggcgg cacggtacac agcgatgaga ttgccagcca 4921 cggtcgtcag cattcactaa gcctgacgct accaccgctg gccactatct ggctggttcg 4981 ggaggcagaa taataa //