BBa_K118016 1 BBa_K118016 glgC16 (glgC with G336D substitution) 2008-10-05T11:00:00Z 2015-05-08T01:09:37Z ''Escherichia coli'' JM109 genomic DNA Released HQ 2013 This is the coding sequence of ''glgC'' (ADP-glucose pyrophosphorylase) from ''Escherichia coli'' JM109 with the substitution G336D. This mutation is known to cause increased activity of ADP-glucose pyrophosphorylase in the absence of the activator fructose 1,6-bisphosphate (FBP), high affinity for FBP and substrates lower affinity for the inhibitor AMP. (Leung ''et al'', 1986; Meyer ''et al.'', 1988) false false _192_ 0 3282 9 In stock true The mutation G336D was achieved by the substitution of A for G at position 1076 (codon GGC->GAC) by MABEL. true Andrew Hall annotation1978558 1 EcoRI site removed range1978558 1 571 576 annotation1978559 1 EcoRI site removed range1978559 1 1068 1073 annotation1978557 1 G336D range1978557 1 1006 1008 BBa_K1585311 1 BBa_K1585311 B0034.GlgB 2015-08-24T11:00:00Z 2015-09-16T11:17:55Z E. coli This is the translational unit of GlgB (glycogen branching enzyme) from E. coli. The enzyme catalyzes the formation of alpha-1,6-branches in glycogen synthesis. false false _2002_ 24469 24505 9 false - false Sarah Lubjuhn, Laura Helleckes, Michael Osthege component2437911 1 BBa_B0034 component2437912 1 BBa_K1585301 annotation2437912 1 BBa_K1585301 range2437912 1 19 2208 annotation2437911 1 BBa_B0034 range2437911 1 1 12 BBa_K1585312 1 BBa_K1585312 B0034.GlgC 2015-08-24T11:00:00Z 2015-09-17T02:21:03Z E. coli This is the translational unit of GlgC. It creates ADP-glucose from glucose-1-phosphate for glycogen synthesis. false false _2002_ 24474 24505 9 false - false Michael Osthege, Sarah Lubjuhn, Laura Helleckes component2437914 1 BBa_B0034 component2437918 1 BBa_K118016 annotation2437914 1 BBa_B0034 range2437914 1 1 12 annotation2437918 1 BBa_K118016 range2437918 1 19 1317 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1585301 1 BBa_K1585301 GlgB 2015-08-24T11:00:00Z 2015-08-25T06:54:01Z E.coli branching enzyme; synthesizes branches in glycogen; from E.coli false false _2002_ 24470 24470 9 false - false Carolin Gr??tering BBa_K1585310 1 BBa_K1585310 B0034.GlgA 2015-08-24T11:00:00Z 2015-09-17T02:20:38Z E. coli This is the translational unit of GlgA (Glycogen synthase) for elongation of glycogen chains. Through autophosphorylation, GlgA is also the starting point of glycogen synthesis in bacteria. false false _2002_ 24474 24505 9 false - false Laura Helleckes, Michael Osthege, Sarah Lubjuhn component2437901 1 BBa_B0034 component2437909 1 BBa_K1585300 annotation2437901 1 BBa_B0034 range2437901 1 1 12 annotation2437909 1 BBa_K1585300 range2437909 1 19 1455 BBa_K1585300 1 BBa_K1585300 Glycogen synthase GlgA from E. coli K12 2015-08-22T11:00:00Z 2015-08-23T02:08:19Z The BioBrick was created from the genomic sequence of glgA in ''E.&nbsp;coli''&nbsp;K12. The glycogen synthase synthesizes alpha-1,4-glucan chains using ADP-glucose. The BioBrick was created from the genomic sequence of glgA in ''E.&nbsp;coli''&nbsp;K12. Six illegal restriction sites were removed to ensure full BioBrick compability. false false _2002_ 19522 19522 9 false Six illegal restriction sites were removed to ensure full BioBrick compability. The native stop codon was replaced with TAATAA. false Michael Osthege annotation2436886 1 glgA range2436886 1 1 1437 annotation2436889 1 Replaced C with T to remove EcoRI site range2436889 1 573 573 annotation2436888 1 Replaced G with C to remove AvrII site range2436888 1 405 405 annotation2436891 1 Replaced G with A to remove PstI site range2436891 1 1005 1005 annotation2436890 1 Replaced G with A to remove PvuII site range2436890 1 969 969 annotation2436887 1 Replaced G with C to remove AviII site range2436887 1 162 162 annotation2436892 1 Replaced T with C to remove AgeI site range2436892 1 1197 1197 BBa_K1585321 1 BBa_K1585321 glgCAB for polycistronic expression of glycogen synthesis genes in E.coli 2015-08-24T11:00:00Z 2015-09-18T06:07:57Z E. coli This composite part combines all 3 enzymes enzymes involved in glycogen synthesis. GlgC creates ADP-glucose from glucose-1-phosphate, GlgA elongates alpha-1,4-linked chains and GlgB catalyzes the formation of alpha-1,6-linked branches. false false _2002_ 24505 24505 9 true - false Sarah Lubjuhn, Laura Helleckes, Michael Osthege component2437951 1 BBa_K1585310 component2437940 1 BBa_K1585312 component2437955 1 BBa_K1585311 annotation2437955 1 BBa_K1585311 range2437955 1 2789 4996 annotation2437940 1 BBa_K1585312 range2437940 1 1 1317 annotation2437951 1 BBa_K1585310 range2437951 1 1326 2780 BBa_K1585300_sequence 1 atgcaggttttacatgtatgttcagagatgttcccgctgcttaaaaccggcggtctggctgatgttattggggcattacccgcagcacaaatcgcagacggcgttgacgctcgcgtactgttgcctgcatttcccgatattcgccgtggcgtgaccgatgcccaggtagtatcccgtcgtgataccttcgccggacatatcacgctgttgttcggtcattacaacggggttggcatttacctgattgacgcgccgcatctctatgatcgtccgggaagcccgtatcacgataccaacttatttgcctataccgacaacgtattgcgttttgcgctgctggggtgggttggggcagaaatggccagcgggcttgacccattctggcgtcctgatgtggtgcatgcccacgactggcatgcaggccttgcgcctgcgtatctggcggcgcgcgggcgtccggcgaagtcggtgtttactgtgcacaacctggcctatcaaggcatgttttatgcacatcacatgaatgacatccaattgccatggtcattctttaatattcatgggctggaatttaacggacaaatctctttcctgaaggccggtctgtactatgccgatcacattacggcggtcagtccaacctacgctcgcgagatcaccgaaccgcagtttgcctacggtatggaaggtctgttgcaacagcgtcaccgtgaagggcgtctttccggcgtactgaacggcgtggacgagaaaatctggagtccagagacggacttactgttggcctcgcgttacacccgcgatacgttggaagataaagcggaaaataagcgccagttacaaatcgcaatggggcttaaggttgacgataaagtgccgctttttgcagtggtgagccgtctgaccagccagaaaggtctcgacctggtgctggaagccttaccgggtcttctggagcagggcgggcaactggcgctactcggcgcgggcgatccggtgctgcaagaaggtttccttgcggcggcagcggaataccccggtcaggtgggcgttcagattggctatcacgaagcattttcgcatcgcattatgggcggcgcggacgtcattctggtgcccagccgttttgaaccgtgcggcttaacgcaactttatggattgaagtacggtacgctgccgttagtgcggcgcaccggcgggcttgctgatacggtttctgactgttctcttgagaaccttgcagatggcgtcgccagtgggtttgtctttgaagatagtaatgcctggtcgctgttacgggctattcgacgtgcttttgtactgtggtcccgtccttcactgtggcggtttgtgcaacgtcaggctatggcaatggattttagctggcaggtcgcggcgaagtcgtaccgtgagctttactatcgcttgaaataataa BBa_K1585312_sequence 1 aaagaggagaaatactagatggttagtttagagaagaacgatcacttaatgttggcgcgccagctgccattgaaatctgttgccctgatactggcgggaggacgtggtacccgcctgaaggatttaaccaataagcgagcaaaaccggccgtacacttcggcggtaagttccgcattatcgactttgcgctgtctaactgcatcaactccgggatccgtcgtatgggcgtgatcacccagtaccagtcccacactctggtgcagcacattcagcgcggctggtcattcttcaatgaagaaatgaacgagtttgtcgatctgctgccagcacagcagagaatgaaaggggaaaactggtatcgcggcaccgcagatgcggtcacccaaaacctcgacattatccgccgttataaagcggaatacgtggtgatcctggcgggcgaccatatctacaagcaagactactcgcgtatgcttatcgatcacgtcgaaaaaggcgcacgttgcaccgttgcttgtatgccagtaccgattgaagaagcctccgcatttggcgttatggcggttgatgagaacgataaaattatcgaatttgttgaaaaacctgctaacccgccgtcaatgccgaacgatccgagcaaatctctggcgagtatgggtatctacgtctttgacgccgactatctgtatgaactgctggaagaagacgatcgcgatgagaactccagccacgactttggcaaagatttgattcccaagatcaccgaagccggtctggcctatgcgcacccgttcccgctctcttgcgtacaatccgacccggatgccgagccgtactggcgcgatgtgggtacgctggaagcttactggaaagcgaacctcgatctggcctctgtggtgccggaactggatatgtacgatcgcaattggccaattcgcacctacaatgaatcattaccgccagcgaaattcgtgcaggatcgctccggtagccacgggatgacccttaactcactggtttccgacggttgtgtgatctccggttcggtggtggtgcagtccgttctgttctcgcgcgttcgcgtgaactcattctgcaacattgattccgccgtattgttaccggaagtatgggtaggtcgctcgtgccgtctgcgccgctgcgtcatcgatcgtgcttgtgttattccggaaggcatggtgattggtgaaaacgcagaggaagatgcacgtcgtttctatcgttcagaagaaggcatcgtgctggtaacgcgcgaaatgctacggaagttagggcataaacaggagcgataataa BBa_K1585311_sequence 1 aaagaggagaaatactagatgtccgatcgtatcgatagagacgtgattaacgcgctaattgcaggccattttgccgatcctttttccgtactgggaatgcataaaaccaccgcgggactggaagtccgtgcccttttacccgacgctaccgatgtgtgggtgattgaaccgaaaaccgggcgcaaactcgcaaaactggagtgtctcgactcacggggattctttagcggcgtcattccgcgacgtaagaattttttccgctatcagttggctgttgtctggcatggtcagcaaaacctgattgatgatccttaccgttttggtccgctaatccaggaaatggatgcctggctattatctgaaggtactcacctgcgcccgtatgaaaccttaggcgcgcatgcagatactatggatggcgtcacaggtacgcgtttctctgtctgggctccaaacgcccgtcgggtctcggtggttgggcaattcaactactgggacggtcgccgtcacccgatgcgcctgcgtaaagagagcggcatctgggaactgtttatccctggggcgcataacggtcagctctataaatacgagatgattgatgccaatggcaacttgcgtctgaagtccgacccttatgcctttgaagcgcaaatgcgcccggaaaccgcgtctcttatttgcgggctgccggaaaaggttgtacagactgaagaacgcaaaaaagcgaatcagtttgatgcgccaatctctatttatgaagttcacctgggttcctggcgtcgccacaccgacaacaatttctggttgagctaccgcgagctggccgatcaactggtgccttatgctaaatggatgggctttacccacctcgaactactgcccattaacgagcatcccttcgatggcagttggggttatcagccaaccggcctgtatgcgccaacccgccgttttggtactcgcgacgacttccgttatttcattgatgccgcacacgcggctggtctgaacgtgattctcgactgggtgccaggccacttcccgactgatgactttgcgcttgccgaatttgatggcacgaacttgtatgaacacagcgatccgcgtgaaggctatcatcaggactggaacacgctgatctacaactatggtcgccgtgaagtcagtaacttcctcgtcggtaacgcgctttactggattgaacgttttggtattgatgcgctgcgcgtcgatgcggtggcgtcaatgatttatcgcgactacagccgtaaagagggggagtggattccgaacgaatttggcgggcgcgagaatcttgaagcgattgaatttttgcgtaataccaaccgtattcttggtgagcaggtttccggtgcggtgacaatggctgaggagtctaccgatttccctggcgtttctcgtccgcaggatatgggcggtctgggcttctggtacaagtggaacctcggctggatgcatgacaccctggactacatgaagctcgacccggtttatcgtcagtatcatcacgataaactgaccttcgggattctctacaactacactgaaaacttcgtcctgccgttgtcgcatgatgaagtggtccacggtaaaaaatcgattctcgaccgcatgccgggcgacgcatggcagaaattcgcgaacctgcgcgcctactatggctggatgtgggcattcccgggcaagaaactactgttcatgggtaacgaatttgcccagggccgcgagtggaaccatgacgccagcctcgactggcatctgttggaaggcggcgataactggcaccacggtgtccagcgtctggtgcgcgatctgaacctcacctaccgccaccataaagcaatgcatgaactggattttgacccgtacggctttgaatggctggtggtggatgacaaagaacgctcggtgctgatctttgtgcgtcgcgataaagagggtaacgaaatcatcgttgccagtaactttacgccggtaccgcgtcatgattatcgcttcggcataaaccagccgggcaaatggcgtgaaatcctcaataccgattccatgcactatcacggcagtaatgcaggcaatggcggcacggtacacagcgatgagattgccagccacggtcgtcagcattcactaagcctgacgctaccaccgctggccactatctggctggttcgggaggcagaataataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K1585310_sequence 1 aaagaggagaaatactagatgcaggttttacatgtatgttcagagatgttcccgctgcttaaaaccggcggtctggctgatgttattggggcattacccgcagcacaaatcgcagacggcgttgacgctcgcgtactgttgcctgcatttcccgatattcgccgtggcgtgaccgatgcccaggtagtatcccgtcgtgataccttcgccggacatatcacgctgttgttcggtcattacaacggggttggcatttacctgattgacgcgccgcatctctatgatcgtccgggaagcccgtatcacgataccaacttatttgcctataccgacaacgtattgcgttttgcgctgctggggtgggttggggcagaaatggccagcgggcttgacccattctggcgtcctgatgtggtgcatgcccacgactggcatgcaggccttgcgcctgcgtatctggcggcgcgcgggcgtccggcgaagtcggtgtttactgtgcacaacctggcctatcaaggcatgttttatgcacatcacatgaatgacatccaattgccatggtcattctttaatattcatgggctggaatttaacggacaaatctctttcctgaaggccggtctgtactatgccgatcacattacggcggtcagtccaacctacgctcgcgagatcaccgaaccgcagtttgcctacggtatggaaggtctgttgcaacagcgtcaccgtgaagggcgtctttccggcgtactgaacggcgtggacgagaaaatctggagtccagagacggacttactgttggcctcgcgttacacccgcgatacgttggaagataaagcggaaaataagcgccagttacaaatcgcaatggggcttaaggttgacgataaagtgccgctttttgcagtggtgagccgtctgaccagccagaaaggtctcgacctggtgctggaagccttaccgggtcttctggagcagggcgggcaactggcgctactcggcgcgggcgatccggtgctgcaagaaggtttccttgcggcggcagcggaataccccggtcaggtgggcgttcagattggctatcacgaagcattttcgcatcgcattatgggcggcgcggacgtcattctggtgcccagccgttttgaaccgtgcggcttaacgcaactttatggattgaagtacggtacgctgccgttagtgcggcgcaccggcgggcttgctgatacggtttctgactgttctcttgagaaccttgcagatggcgtcgccagtgggtttgtctttgaagatagtaatgcctggtcgctgttacgggctattcgacgtgcttttgtactgtggtcccgtccttcactgtggcggtttgtgcaacgtcaggctatggcaatggattttagctggcaggtcgcggcgaagtcgtaccgtgagctttactatcgcttgaaataataa BBa_K1585321_sequence 1 aaagaggagaaatactagatggttagtttagagaagaacgatcacttaatgttggcgcgccagctgccattgaaatctgttgccctgatactggcgggaggacgtggtacccgcctgaaggatttaaccaataagcgagcaaaaccggccgtacacttcggcggtaagttccgcattatcgactttgcgctgtctaactgcatcaactccgggatccgtcgtatgggcgtgatcacccagtaccagtcccacactctggtgcagcacattcagcgcggctggtcattcttcaatgaagaaatgaacgagtttgtcgatctgctgccagcacagcagagaatgaaaggggaaaactggtatcgcggcaccgcagatgcggtcacccaaaacctcgacattatccgccgttataaagcggaatacgtggtgatcctggcgggcgaccatatctacaagcaagactactcgcgtatgcttatcgatcacgtcgaaaaaggcgcacgttgcaccgttgcttgtatgccagtaccgattgaagaagcctccgcatttggcgttatggcggttgatgagaacgataaaattatcgaatttgttgaaaaacctgctaacccgccgtcaatgccgaacgatccgagcaaatctctggcgagtatgggtatctacgtctttgacgccgactatctgtatgaactgctggaagaagacgatcgcgatgagaactccagccacgactttggcaaagatttgattcccaagatcaccgaagccggtctggcctatgcgcacccgttcccgctctcttgcgtacaatccgacccggatgccgagccgtactggcgcgatgtgggtacgctggaagcttactggaaagcgaacctcgatctggcctctgtggtgccggaactggatatgtacgatcgcaattggccaattcgcacctacaatgaatcattaccgccagcgaaattcgtgcaggatcgctccggtagccacgggatgacccttaactcactggtttccgacggttgtgtgatctccggttcggtggtggtgcagtccgttctgttctcgcgcgttcgcgtgaactcattctgcaacattgattccgccgtattgttaccggaagtatgggtaggtcgctcgtgccgtctgcgccgctgcgtcatcgatcgtgcttgtgttattccggaaggcatggtgattggtgaaaacgcagaggaagatgcacgtcgtttctatcgttcagaagaaggcatcgtgctggtaacgcgcgaaatgctacggaagttagggcataaacaggagcgataataatactagagaaagaggagaaatactagatgcaggttttacatgtatgttcagagatgttcccgctgcttaaaaccggcggtctggctgatgttattggggcattacccgcagcacaaatcgcagacggcgttgacgctcgcgtactgttgcctgcatttcccgatattcgccgtggcgtgaccgatgcccaggtagtatcccgtcgtgataccttcgccggacatatcacgctgttgttcggtcattacaacggggttggcatttacctgattgacgcgccgcatctctatgatcgtccgggaagcccgtatcacgataccaacttatttgcctataccgacaacgtattgcgttttgcgctgctggggtgggttggggcagaaatggccagcgggcttgacccattctggcgtcctgatgtggtgcatgcccacgactggcatgcaggccttgcgcctgcgtatctggcggcgcgcgggcgtccggcgaagtcggtgtttactgtgcacaacctggcctatcaaggcatgttttatgcacatcacatgaatgacatccaattgccatggtcattctttaatattcatgggctggaatttaacggacaaatctctttcctgaaggccggtctgtactatgccgatcacattacggcggtcagtccaacctacgctcgcgagatcaccgaaccgcagtttgcctacggtatggaaggtctgttgcaacagcgtcaccgtgaagggcgtctttccggcgtactgaacggcgtggacgagaaaatctggagtccagagacggacttactgttggcctcgcgttacacccgcgatacgttggaagataaagcggaaaataagcgccagttacaaatcgcaatggggcttaaggttgacgataaagtgccgctttttgcagtggtgagccgtctgaccagccagaaaggtctcgacctggtgctggaagccttaccgggtcttctggagcagggcgggcaactggcgctactcggcgcgggcgatccggtgctgcaagaaggtttccttgcggcggcagcggaataccccggtcaggtgggcgttcagattggctatcacgaagcattttcgcatcgcattatgggcggcgcggacgtcattctggtgcccagccgttttgaaccgtgcggcttaacgcaactttatggattgaagtacggtacgctgccgttagtgcggcgcaccggcgggcttgctgatacggtttctgactgttctcttgagaaccttgcagatggcgtcgccagtgggtttgtctttgaagatagtaatgcctggtcgctgttacgggctattcgacgtgcttttgtactgtggtcccgtccttcactgtggcggtttgtgcaacgtcaggctatggcaatggattttagctggcaggtcgcggcgaagtcgtaccgtgagctttactatcgcttgaaataataatactagagaaagaggagaaatactagatgtccgatcgtatcgatagagacgtgattaacgcgctaattgcaggccattttgccgatcctttttccgtactgggaatgcataaaaccaccgcgggactggaagtccgtgcccttttacccgacgctaccgatgtgtgggtgattgaaccgaaaaccgggcgcaaactcgcaaaactggagtgtctcgactcacggggattctttagcggcgtcattccgcgacgtaagaattttttccgctatcagttggctgttgtctggcatggtcagcaaaacctgattgatgatccttaccgttttggtccgctaatccaggaaatggatgcctggctattatctgaaggtactcacctgcgcccgtatgaaaccttaggcgcgcatgcagatactatggatggcgtcacaggtacgcgtttctctgtctgggctccaaacgcccgtcgggtctcggtggttgggcaattcaactactgggacggtcgccgtcacccgatgcgcctgcgtaaagagagcggcatctgggaactgtttatccctggggcgcataacggtcagctctataaatacgagatgattgatgccaatggcaacttgcgtctgaagtccgacccttatgcctttgaagcgcaaatgcgcccggaaaccgcgtctcttatttgcgggctgccggaaaaggttgtacagactgaagaacgcaaaaaagcgaatcagtttgatgcgccaatctctatttatgaagttcacctgggttcctggcgtcgccacaccgacaacaatttctggttgagctaccgcgagctggccgatcaactggtgccttatgctaaatggatgggctttacccacctcgaactactgcccattaacgagcatcccttcgatggcagttggggttatcagccaaccggcctgtatgcgccaacccgccgttttggtactcgcgacgacttccgttatttcattgatgccgcacacgcggctggtctgaacgtgattctcgactgggtgccaggccacttcccgactgatgactttgcgcttgccgaatttgatggcacgaacttgtatgaacacagcgatccgcgtgaaggctatcatcaggactggaacacgctgatctacaactatggtcgccgtgaagtcagtaacttcctcgtcggtaacgcgctttactggattgaacgttttggtattgatgcgctgcgcgtcgatgcggtggcgtcaatgatttatcgcgactacagccgtaaagagggggagtggattccgaacgaatttggcgggcgcgagaatcttgaagcgattgaatttttgcgtaataccaaccgtattcttggtgagcaggtttccggtgcggtgacaatggctgaggagtctaccgatttccctggcgtttctcgtccgcaggatatgggcggtctgggcttctggtacaagtggaacctcggctggatgcatgacaccctggactacatgaagctcgacccggtttatcgtcagtatcatcacgataaactgaccttcgggattctctacaactacactgaaaacttcgtcctgccgttgtcgcatgatgaagtggtccacggtaaaaaatcgattctcgaccgcatgccgggcgacgcatggcagaaattcgcgaacctgcgcgcctactatggctggatgtgggcattcccgggcaagaaactactgttcatgggtaacgaatttgcccagggccgcgagtggaaccatgacgccagcctcgactggcatctgttggaaggcggcgataactggcaccacggtgtccagcgtctggtgcgcgatctgaacctcacctaccgccaccataaagcaatgcatgaactggattttgacccgtacggctttgaatggctggtggtggatgacaaagaacgctcggtgctgatctttgtgcgtcgcgataaagagggtaacgaaatcatcgttgccagtaactttacgccggtaccgcgtcatgattatcgcttcggcataaaccagccgggcaaatggcgtgaaatcctcaataccgattccatgcactatcacggcagtaatgcaggcaatggcggcacggtacacagcgatgagattgccagccacggtcgtcagcattcactaagcctgacgctaccaccgctggccactatctggctggttcgggaggcagaataataa BBa_K1585301_sequence 1 atgtccgatcgtatcgatagagacgtgattaacgcgctaattgcaggccattttgccgatcctttttccgtactgggaatgcataaaaccaccgcgggactggaagtccgtgcccttttacccgacgctaccgatgtgtgggtgattgaaccgaaaaccgggcgcaaactcgcaaaactggagtgtctcgactcacggggattctttagcggcgtcattccgcgacgtaagaattttttccgctatcagttggctgttgtctggcatggtcagcaaaacctgattgatgatccttaccgttttggtccgctaatccaggaaatggatgcctggctattatctgaaggtactcacctgcgcccgtatgaaaccttaggcgcgcatgcagatactatggatggcgtcacaggtacgcgtttctctgtctgggctccaaacgcccgtcgggtctcggtggttgggcaattcaactactgggacggtcgccgtcacccgatgcgcctgcgtaaagagagcggcatctgggaactgtttatccctggggcgcataacggtcagctctataaatacgagatgattgatgccaatggcaacttgcgtctgaagtccgacccttatgcctttgaagcgcaaatgcgcccggaaaccgcgtctcttatttgcgggctgccggaaaaggttgtacagactgaagaacgcaaaaaagcgaatcagtttgatgcgccaatctctatttatgaagttcacctgggttcctggcgtcgccacaccgacaacaatttctggttgagctaccgcgagctggccgatcaactggtgccttatgctaaatggatgggctttacccacctcgaactactgcccattaacgagcatcccttcgatggcagttggggttatcagccaaccggcctgtatgcgccaacccgccgttttggtactcgcgacgacttccgttatttcattgatgccgcacacgcggctggtctgaacgtgattctcgactgggtgccaggccacttcccgactgatgactttgcgcttgccgaatttgatggcacgaacttgtatgaacacagcgatccgcgtgaaggctatcatcaggactggaacacgctgatctacaactatggtcgccgtgaagtcagtaacttcctcgtcggtaacgcgctttactggattgaacgttttggtattgatgcgctgcgcgtcgatgcggtggcgtcaatgatttatcgcgactacagccgtaaagagggggagtggattccgaacgaatttggcgggcgcgagaatcttgaagcgattgaatttttgcgtaataccaaccgtattcttggtgagcaggtttccggtgcggtgacaatggctgaggagtctaccgatttccctggcgtttctcgtccgcaggatatgggcggtctgggcttctggtacaagtggaacctcggctggatgcatgacaccctggactacatgaagctcgacccggtttatcgtcagtatcatcacgataaactgaccttcgggattctctacaactacactgaaaacttcgtcctgccgttgtcgcatgatgaagtggtccacggtaaaaaatcgattctcgaccgcatgccgggcgacgcatggcagaaattcgcgaacctgcgcgcctactatggctggatgtgggcattcccgggcaagaaactactgttcatgggtaacgaatttgcccagggccgcgagtggaaccatgacgccagcctcgactggcatctgttggaaggcggcgataactggcaccacggtgtccagcgtctggtgcgcgatctgaacctcacctaccgccaccataaagcaatgcatgaactggattttgacccgtacggctttgaatggctggtggtggatgacaaagaacgctcggtgctgatctttgtgcgtcgcgataaagagggtaacgaaatcatcgttgccagtaactttacgccggtaccgcgtcatgattatcgcttcggcataaaccagccgggcaaatggcgtgaaatcctcaataccgattccatgcactatcacggcagtaatgcaggcaatggcggcacggtacacagcgatgagattgccagccacggtcgtcagcattcactaagcctgacgctaccaccgctggccactatctggctggttcgggaggcagaataataa BBa_K118016_sequence 1 atggttagtttagagaagaacgatcacttaatgttggcgcgccagctgccattgaaatctgttgccctgatactggcgggaggacgtggtacccgcctgaaggatttaaccaataagcgagcaaaaccggccgtacacttcggcggtaagttccgcattatcgactttgcgctgtctaactgcatcaactccgggatccgtcgtatgggcgtgatcacccagtaccagtcccacactctggtgcagcacattcagcgcggctggtcattcttcaatgaagaaatgaacgagtttgtcgatctgctgccagcacagcagagaatgaaaggggaaaactggtatcgcggcaccgcagatgcggtcacccaaaacctcgacattatccgccgttataaagcggaatacgtggtgatcctggcgggcgaccatatctacaagcaagactactcgcgtatgcttatcgatcacgtcgaaaaaggcgcacgttgcaccgttgcttgtatgccagtaccgattgaagaagcctccgcatttggcgttatggcggttgatgagaacgataaaattatcgaatttgttgaaaaacctgctaacccgccgtcaatgccgaacgatccgagcaaatctctggcgagtatgggtatctacgtctttgacgccgactatctgtatgaactgctggaagaagacgatcgcgatgagaactccagccacgactttggcaaagatttgattcccaagatcaccgaagccggtctggcctatgcgcacccgttcccgctctcttgcgtacaatccgacccggatgccgagccgtactggcgcgatgtgggtacgctggaagcttactggaaagcgaacctcgatctggcctctgtggtgccggaactggatatgtacgatcgcaattggccaattcgcacctacaatgaatcattaccgccagcgaaattcgtgcaggatcgctccggtagccacgggatgacccttaactcactggtttccgacggttgtgtgatctccggttcggtggtggtgcagtccgttctgttctcgcgcgttcgcgtgaactcattctgcaacattgattccgccgtattgttaccggaagtatgggtaggtcgctcgtgccgtctgcgccgctgcgtcatcgatcgtgcttgtgttattccggaaggcatggtgattggtgaaaacgcagaggaagatgcacgtcgtttctatcgttcagaagaaggcatcgtgctggtaacgcgcgaaatgctacggaagttagggcataaacaggagcgataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z