BBa_K1585321
type: ComponentDefinition
persistent identity: BBa K1585321
display id: BBa_K1585321
version: 1
was generated by: iGEM to SBOL conversion
description: glgCAB for polycistronic expression of glycogen synthesis genes in E.coli
created: 2015-08-24T11:00:00Z
modified: 2015-09-18T06:07:57Z
mutable provenance: E. coli
owned by: james
owned by: myers
mutable description: This composite part combines all 3 enzymes enzymes involved in glycogen synthesis. GlgC creates ADP-glucose from glucose-1-phosphate, GlgA elongates alpha-1,4-linked chains and GlgB catalyzes the formation of alpha-1,6-linked branches.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2002_
m user id: 24505
owner id: 24505
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: -
star: false
creator: Sarah Lubjuhn, Laura Helleckes, Michael Osthege
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1585311
component: BBa_K1585310
component: BBa_K1585312
sequence annotation: BBa_K1585312
sequence annotation: BBa_K1585310
sequence annotation: BBa_K1585311
sequence: https://synbiohub.org/public/igem/BBa_K1585321_sequence/1

BBa_K1585311
type: Component
persistent identity: component2437955
display id: component2437955
version: 1
top level: BBa_K1585321
definition: BBa_K1585311
access: public

BBa_K1585310
type: Component
persistent identity: component2437951
display id: component2437951
version: 1
top level: BBa_K1585321
definition: BBa_K1585310
access: public

BBa_K1585312
type: Component
persistent identity: component2437940
display id: component2437940
version: 1
top level: BBa_K1585321
definition: BBa_K1585312
access: public

BBa_K1585312
type: SequenceAnnotation
persistent identity: annotation2437940
display id: annotation2437940
version: 1
top level: BBa_K1585321
location: https://synbiohub.org/public/igem/BBa_K1585321/annotation2437940/range2437940/1
component: BBa_K1585312

https://synbiohub.org/public/igem/BBa_K1585321/annotation2437940/range2437940/1
type: Range
persistent identity: range2437940
display id: range2437940
version: 1
top level: BBa_K1585321
direction: direction/reverse
start: 1
end: 1317
orientation: inline

BBa_K1585310
type: SequenceAnnotation
persistent identity: annotation2437951
display id: annotation2437951
version: 1
top level: BBa_K1585321
location: https://synbiohub.org/public/igem/BBa_K1585321/annotation2437951/range2437951/1
component: BBa_K1585310

https://synbiohub.org/public/igem/BBa_K1585321/annotation2437951/range2437951/1
type: Range
persistent identity: range2437951
display id: range2437951
version: 1
top level: BBa_K1585321
direction: direction/reverse
start: 1326
end: 2780
orientation: inline

BBa_K1585311
type: SequenceAnnotation
persistent identity: annotation2437955
display id: annotation2437955
version: 1
top level: BBa_K1585321
location: https://synbiohub.org/public/igem/BBa_K1585321/annotation2437955/range2437955/1
component: BBa_K1585311

https://synbiohub.org/public/igem/BBa_K1585321/annotation2437955/range2437955/1
type: Range
persistent identity: range2437955
display id: range2437955
version: 1
top level: BBa_K1585321
direction: direction/reverse
start: 2789
end: 4996
orientation: inline

BBa_K1585311
type: ComponentDefinition
persistent identity: BBa K1585311
display id: BBa_K1585311
version: 1
was generated by: iGEM to SBOL conversion
description: B0034.GlgB
created: 2015-08-24T11:00:00Z
modified: 2015-09-16T11:17:55Z
mutable provenance: E. coli
owned by: james
owned by: myers
mutable description: This is the translational unit of GlgB (glycogen branching enzyme) from E. coli. The enzyme catalyzes the formation of alpha-1,6-branches in glycogen synthesis.
discontinued: false
dominant: false
experience: experience/None
group u list: _2002_
m user id: 24469
owner id: 24505
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Sarah Lubjuhn, Laura Helleckes, Michael Osthege
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0034
component: BBa_K1585301
sequence annotation: BBa_B0034
sequence annotation: BBa_K1585301
sequence: https://synbiohub.org/public/igem/BBa_K1585311_sequence/1

BBa_B0034
type: Component
persistent identity: component2437911
display id: component2437911
version: 1
top level: BBa_K1585311
definition: BBa_B0034
access: public

BBa_K1585301
type: Component
persistent identity: component2437912
display id: component2437912
version: 1
top level: BBa_K1585311
definition: BBa_K1585301
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2437911
display id: annotation2437911
version: 1
top level: BBa_K1585311
location: https://synbiohub.org/public/igem/BBa_K1585311/annotation2437911/range2437911/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1585311/annotation2437911/range2437911/1
type: Range
persistent identity: range2437911
display id: range2437911
version: 1
top level: BBa_K1585311
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1585301
type: SequenceAnnotation
persistent identity: annotation2437912
display id: annotation2437912
version: 1
top level: BBa_K1585311
location: https://synbiohub.org/public/igem/BBa_K1585311/annotation2437912/range2437912/1
component: BBa_K1585301

https://synbiohub.org/public/igem/BBa_K1585311/annotation2437912/range2437912/1
type: Range
persistent identity: range2437912
display id: range2437912
version: 1
top level: BBa_K1585311
direction: direction/reverse
start: 19
end: 2208
orientation: inline

BBa_K1585310
type: ComponentDefinition
persistent identity: BBa K1585310
display id: BBa_K1585310
version: 1
was generated by: iGEM to SBOL conversion
description: B0034.GlgA
created: 2015-08-24T11:00:00Z
modified: 2015-09-17T02:20:38Z
mutable provenance: E. coli
owned by: james
owned by: myers
mutable description: This is the translational unit of GlgA (Glycogen synthase) for elongation of glycogen chains. Through autophosphorylation, GlgA is also the starting point of glycogen synthesis in bacteria.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2002_
m user id: 24474
owner id: 24505
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Laura Helleckes, Michael Osthege, Sarah Lubjuhn
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1585300
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K1585300
sequence: https://synbiohub.org/public/igem/BBa_K1585310_sequence/1

BBa_K1585300
type: Component
persistent identity: component2437909
display id: component2437909
version: 1
top level: BBa_K1585310
definition: BBa_K1585300
access: public

BBa_B0034
type: Component
persistent identity: component2437901
display id: component2437901
version: 1
top level: BBa_K1585310
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2437901
display id: annotation2437901
version: 1
top level: BBa_K1585310
location: https://synbiohub.org/public/igem/BBa_K1585310/annotation2437901/range2437901/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1585310/annotation2437901/range2437901/1
type: Range
persistent identity: range2437901
display id: range2437901
version: 1
top level: BBa_K1585310
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1585300
type: SequenceAnnotation
persistent identity: annotation2437909
display id: annotation2437909
version: 1
top level: BBa_K1585310
location: https://synbiohub.org/public/igem/BBa_K1585310/annotation2437909/range2437909/1
component: BBa_K1585300

https://synbiohub.org/public/igem/BBa_K1585310/annotation2437909/range2437909/1
type: Range
persistent identity: range2437909
display id: range2437909
version: 1
top level: BBa_K1585310
direction: direction/reverse
start: 19
end: 1455
orientation: inline

BBa_K1585312
type: ComponentDefinition
persistent identity: BBa K1585312
display id: BBa_K1585312
version: 1
was generated by: iGEM to SBOL conversion
description: B0034.GlgC
created: 2015-08-24T11:00:00Z
modified: 2015-09-17T02:21:03Z
mutable provenance: E. coli
owned by: james
owned by: myers
mutable description: This is the translational unit of GlgC. It creates ADP-glucose from glucose-1-phosphate for glycogen synthesis.
discontinued: false
dominant: false
experience: experience/Works
group u list: _2002_
m user id: 24474
owner id: 24505
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Michael Osthege, Sarah Lubjuhn, Laura Helleckes
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0034
component: BBa_K118016
sequence annotation: BBa_B0034
sequence annotation: BBa_K118016
sequence: https://synbiohub.org/public/igem/BBa_K1585312_sequence/1

BBa_B0034
type: Component
persistent identity: component2437914
display id: component2437914
version: 1
top level: BBa_K1585312
definition: BBa_B0034
access: public

BBa_K118016
type: Component
persistent identity: component2437918
display id: component2437918
version: 1
top level: BBa_K1585312
definition: BBa_K118016
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2437914
display id: annotation2437914
version: 1
top level: BBa_K1585312
location: https://synbiohub.org/public/igem/BBa_K1585312/annotation2437914/range2437914/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1585312/annotation2437914/range2437914/1
type: Range
persistent identity: range2437914
display id: range2437914
version: 1
top level: BBa_K1585312
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K118016
type: SequenceAnnotation
persistent identity: annotation2437918
display id: annotation2437918
version: 1
top level: BBa_K1585312
location: https://synbiohub.org/public/igem/BBa_K1585312/annotation2437918/range2437918/1
component: BBa_K118016

https://synbiohub.org/public/igem/BBa_K1585312/annotation2437918/range2437918/1
type: Range
persistent identity: range2437918
display id: range2437918
version: 1
top level: BBa_K1585312
direction: direction/reverse
start: 19
end: 1317
orientation: inline

BBa_K1585300
type: ComponentDefinition
persistent identity: BBa K1585300
display id: BBa_K1585300
version: 1
was generated by: iGEM to SBOL conversion
description: Glycogen synthase GlgA from E. coli K12
created: 2015-08-22T11:00:00Z
modified: 2015-08-23T02:08:19Z
mutable provenance: The BioBrick was created from the genomic sequence of glgA in ''E. coli'' K12.
owned by: james
owned by: myers
mutable description: The glycogen synthase synthesizes alpha-1,4-glucan chains using ADP-glucose. The BioBrick was created from the genomic sequence of glgA in ''E. coli'' K12. Six illegal restriction sites were removed to ensure full BioBrick compability.
discontinued: false
dominant: false
group u list: _2002_
m user id: 19522
owner id: 19522
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Six illegal restriction sites were removed to ensure full BioBrick compability. The native stop codon was replaced with TAATAA.
star: false
creator: Michael Osthege
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: glgA
sequence annotation: Replaced G with C to remove AviII site
sequence annotation: Replaced G with C to remove AvrII site
sequence annotation: Replaced C with T to remove EcoRI site
sequence annotation: Replaced G with A to remove PvuII site
sequence annotation: Replaced G with A to remove PstI site
sequence annotation: Replaced T with C to remove AgeI site
sequence: https://synbiohub.org/public/igem/BBa_K1585300_sequence/1

glgA
type: SequenceAnnotation
persistent identity: annotation2436886
display id: annotation2436886
version: 1
top level: BBa_K1585300
location: https://synbiohub.org/public/igem/BBa_K1585300/annotation2436886/range2436886/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1585300/annotation2436886/range2436886/1
type: Range
persistent identity: range2436886
display id: range2436886
version: 1
top level: BBa_K1585300
direction: direction/forward
start: 1
end: 1437
orientation: inline

Replaced G with C to remove AviII site
type: SequenceAnnotation
persistent identity: annotation2436887
display id: annotation2436887
version: 1
top level: BBa_K1585300
location: https://synbiohub.org/public/igem/BBa_K1585300/annotation2436887/range2436887/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K1585300/annotation2436887/range2436887/1
type: Range
persistent identity: range2436887
display id: range2436887
version: 1
top level: BBa_K1585300
direction: direction/forward
start: 162
end: 162
orientation: inline

Replaced G with C to remove AvrII site
type: SequenceAnnotation
persistent identity: annotation2436888
display id: annotation2436888
version: 1
top level: BBa_K1585300
location: https://synbiohub.org/public/igem/BBa_K1585300/annotation2436888/range2436888/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K1585300/annotation2436888/range2436888/1
type: Range
persistent identity: range2436888
display id: range2436888
version: 1
top level: BBa_K1585300
direction: direction/forward
start: 405
end: 405
orientation: inline

Replaced C with T to remove EcoRI site
type: SequenceAnnotation
persistent identity: annotation2436889
display id: annotation2436889
version: 1
top level: BBa_K1585300
location: https://synbiohub.org/public/igem/BBa_K1585300/annotation2436889/range2436889/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K1585300/annotation2436889/range2436889/1
type: Range
persistent identity: range2436889
display id: range2436889
version: 1
top level: BBa_K1585300
direction: direction/forward
start: 573
end: 573
orientation: inline

Replaced G with A to remove PvuII site
type: SequenceAnnotation
persistent identity: annotation2436890
display id: annotation2436890
version: 1
top level: BBa_K1585300
location: https://synbiohub.org/public/igem/BBa_K1585300/annotation2436890/range2436890/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K1585300/annotation2436890/range2436890/1
type: Range
persistent identity: range2436890
display id: range2436890
version: 1
top level: BBa_K1585300
direction: direction/forward
start: 969
end: 969
orientation: inline

Replaced G with A to remove PstI site
type: SequenceAnnotation
persistent identity: annotation2436891
display id: annotation2436891
version: 1
top level: BBa_K1585300
location: https://synbiohub.org/public/igem/BBa_K1585300/annotation2436891/range2436891/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K1585300/annotation2436891/range2436891/1
type: Range
persistent identity: range2436891
display id: range2436891
version: 1
top level: BBa_K1585300
direction: direction/forward
start: 1005
end: 1005
orientation: inline

Replaced T with C to remove AgeI site
type: SequenceAnnotation
persistent identity: annotation2436892
display id: annotation2436892
version: 1
top level: BBa_K1585300
location: https://synbiohub.org/public/igem/BBa_K1585300/annotation2436892/range2436892/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K1585300/annotation2436892/range2436892/1
type: Range
persistent identity: range2436892
display id: range2436892
version: 1
top level: BBa_K1585300
direction: direction/forward
start: 1197
end: 1197
orientation: inline

BBa_K1585301
type: ComponentDefinition
persistent identity: BBa K1585301
display id: BBa_K1585301
version: 1
was generated by: iGEM to SBOL conversion
description: GlgB
created: 2015-08-24T11:00:00Z
modified: 2015-08-25T06:54:01Z
mutable provenance: E.coli
owned by: james
owned by: myers
mutable description: branching enzyme; synthesizes branches in glycogen; from E.coli
discontinued: false
dominant: false
group u list: _2002_
m user id: 24470
owner id: 24470
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Carolin Gr??tering
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1585301_sequence/1

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_K118016
type: ComponentDefinition
persistent identity: BBa K118016
display id: BBa_K118016
version: 1
was generated by: iGEM to SBOL conversion
description: glgC16 (glgC with G336D substitution)
created: 2008-10-05T11:00:00Z
modified: 2015-05-08T01:09:37Z
mutable provenance: ''Escherichia coli'' JM109 genomic DNA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the coding sequence of ''glgC'' (ADP-glucose pyrophosphorylase) from ''Escherichia coli'' JM109 with the substitution G336D. This mutation is known to cause increased activity of ADP-glucose pyrophosphorylase in the absence of the activator fructose 1,6-bisphosphate (FBP), high affinity for FBP and substrates lower affinity for the inhibitor AMP. (Leung ''et al'', 1986; Meyer ''et al.'', 1988)
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The mutation G336D was achieved by the substitution of A for G at position 1076 (codon GGC->GAC) by MABEL.
star: true
creator: Andrew Hall
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EcoRI site removed
sequence annotation: G336D
sequence: https://synbiohub.org/public/igem/BBa_K118016_sequence/1

EcoRI site removed
type: SequenceAnnotation
persistent identity: annotation1978558
display id: annotation1978558
version: 1
top level: BBa_K118016
location: https://synbiohub.org/public/igem/BBa_K118016/annotation1978558/range1978558/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K118016/annotation1978558/range1978558/1
type: Range
persistent identity: range1978558
display id: range1978558
version: 1
top level: BBa_K118016
direction: direction/forward
start: 571
end: 576
orientation: inline

G336D
type: SequenceAnnotation
persistent identity: annotation1978557
display id: annotation1978557
version: 1
top level: BBa_K118016
location: https://synbiohub.org/public/igem/BBa_K118016/annotation1978557/range1978557/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K118016/annotation1978557/range1978557/1
type: Range
persistent identity: range1978557
display id: range1978557
version: 1
top level: BBa_K118016
direction: direction/forward
start: 1006
end: 1008
orientation: inline

EcoRI site removed
type: SequenceAnnotation
persistent identity: annotation1978559
display id: annotation1978559
version: 1
top level: BBa_K118016
location: https://synbiohub.org/public/igem/BBa_K118016/annotation1978559/range1978559/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K118016/annotation1978559/range1978559/1
type: Range
persistent identity: range1978559
display id: range1978559
version: 1
top level: BBa_K118016
direction: direction/forward
start: 1068
end: 1073
orientation: inline

https://synbiohub.org/public/igem/BBa_K1585321_sequence/1
type: Sequence
persistent identity: BBa K1585321 sequence
display id: BBa_K1585321_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggttagtttagagaagaacgatcacttaatgttggcgcgccagctgccattgaaatctgttgccctgatactggcgggaggacgtggtacccgcctgaaggatttaaccaataagcgagcaaaaccggccgtacacttcggcggtaagttccgcattatcgactttgcgctgtctaactgcatcaactccgggatccgtcgtatgggcgtgatcacccagtaccagtcccacactctggtgcagcacattcagcgcggctggtcattcttcaatgaagaaatgaacgagtttgtcgatctgctgccagcacagcagagaatgaaaggggaaaactggtatcgcggcaccgcagatgcggtcacccaaaacctcgacattatccgccgttataaagcggaatacgtggtgatcctggcgggcgaccatatctacaagcaagactactcgcgtatgcttatcgatcacgtcgaaaaaggcgcacgttgcaccgttgcttgtatgccagtaccgattgaagaagcctccgcatttggcgttatggcggttgatgagaacgataaaattatcgaatttgttgaaaaacctgctaacccgccgtcaatgccgaacgatccgagcaaatctctggcgagtatgggtatctacgtctttgacgccgactatctgtatgaactgctggaagaagacgatcgcgatgagaactccagccacgactttggcaaagatttgattcccaagatcaccgaagccggtctggcctatgcgcacccgttcccgctctcttgcgtacaatccgacccggatgccgagccgtactggcgcgatgtgggtacgctggaagcttactggaaagcgaacctcgatctggcctctgtggtgccggaactggatatgtacgatcgcaattggccaattcgcacctacaatgaatcattaccgccagcgaaattcgtgcaggatcgctccggtagccacgggatgacccttaactcactggtttccgacggttgtgtgatctccggttcggtggtggtgcagtccgttctgttctcgcgcgttcgcgtgaactcattctgcaacattgattccgccgtattgttaccggaagtatgggtaggtcgctcgtgccgtctgcgccgctgcgtcatcgatcgtgcttgtgttattccggaaggcatggtgattggtgaaaacgcagaggaagatgcacgtcgtttctatcgttcagaagaaggcatcgtgctggtaacgcgcgaaatgctacggaagttagggcataaacaggagcgataataatactagagaaagaggagaaatactagatgcaggttttacatgtatgttcagagatgttcccgctgcttaaaaccggcggtctggctgatgttattggggcattacccgcagcacaaatcgcagacggcgttgacgctcgcgtactgttgcctgcatttcccgatattcgccgtggcgtgaccgatgcccaggtagtatcccgtcgtgataccttcgccggacatatcacgctgttgttcggtcattacaacggggttggcatttacctgattgacgcgccgcatctctatgatcgtccgggaagcccgtatcacgataccaacttatttgcctataccgacaacgtattgcgttttgcgctgctggggtgggttggggcagaaatggccagcgggcttgacccattctggcgtcctgatgtggtgcatgcccacgactggcatgcaggccttgcgcctgcgtatctggcggcgcgcgggcgtccggcgaagtcggtgtttactgtgcacaacctggcctatcaaggcatgttttatgcacatcacatgaatgacatccaattgccatggtcattctttaatattcatgggctggaatttaacggacaaatctctttcctgaaggccggtctgtactatgccgatcacattacggcggtcagtccaacctacgctcgcgagatc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1585310_sequence/1
type: Sequence
persistent identity: BBa K1585310 sequence
display id: BBa_K1585310_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgcaggttttacatgtatgttcagagatgttcccgctgcttaaaaccggcggtctggctgatgttattggggcattacccgcagcacaaatcgcagacggcgttgacgctcgcgtactgttgcctgcatttcccgatattcgccgtggcgtgaccgatgcccaggtagtatcccgtcgtgataccttcgccggacatatcacgctgttgttcggtcattacaacggggttggcatttacctgattgacgcgccgcatctctatgatcgtccgggaagcccgtatcacgataccaacttatttgcctataccgacaacgtattgcgttttgcgctgctggggtgggttggggcagaaatggccagcgggcttgacccattctggcgtcctgatgtggtgcatgcccacgactggcatgcaggccttgcgcctgcgtatctggcggcgcgcgggcgtccggcgaagtcggtgtttactgtgcacaacctggcctatcaaggcatgttttatgcacatcacatgaatgacatccaattgccatggtcattctttaatattcatgggctggaatttaacggacaaatctctttcctgaaggccggtctgtactatgccgatcacattacggcggtcagtccaacctacgctcgcgagatcaccgaaccgcagtttgcctacggtatggaaggtctgttgcaacagcgtcaccgtgaagggcgtctttccggcgtactgaacggcgtggacgagaaaatctggagtccagagacggacttactgttggcctcgcgttacacccgcgatacgttggaagataaagcggaaaataagcgccagttacaaatcgcaatggggcttaaggttgacgataaagtgccgctttttgcagtggtgagccgtctgaccagccagaaaggtctcgacctggtgctggaagccttaccgggtcttctggagcagggcgggcaactggcgctactcggcgcgggcgatccggtgctgcaagaaggtttccttgcggcggcagcggaataccccggtcaggtgggcgttcagattggctatcacgaagcattttcgcatcgcattatgggcggcgcggacgtcattctggtgcccagccgttttgaaccgtgcggcttaacgcaactttatggattgaagtacggtacgctgccgttagtgcggcgcaccggcgggcttgctgatacggtttctgactgttctcttgagaaccttgcagatggcgtcgccagtgggtttgtctttgaagatagtaatgcctggtcgctgttacgggctattcgacgtgcttttgtactgtggtcccgtccttcactgtggcggtttgtgcaacgtcaggctatggcaatggattttagctggcaggtcgcggcgaagtcgtaccgtgagctttactatcgcttgaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1585311_sequence/1
type: Sequence
persistent identity: BBa K1585311 sequence
display id: BBa_K1585311_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtccgatcgtatcgatagagacgtgattaacgcgctaattgcaggccattttgccgatcctttttccgtactgggaatgcataaaaccaccgcgggactggaagtccgtgcccttttacccgacgctaccgatgtgtgggtgattgaaccgaaaaccgggcgcaaactcgcaaaactggagtgtctcgactcacggggattctttagcggcgtcattccgcgacgtaagaattttttccgctatcagttggctgttgtctggcatggtcagcaaaacctgattgatgatccttaccgttttggtccgctaatccaggaaatggatgcctggctattatctgaaggtactcacctgcgcccgtatgaaaccttaggcgcgcatgcagatactatggatggcgtcacaggtacgcgtttctctgtctgggctccaaacgcccgtcgggtctcggtggttgggcaattcaactactgggacggtcgccgtcacccgatgcgcctgcgtaaagagagcggcatctgggaactgtttatccctggggcgcataacggtcagctctataaatacgagatgattgatgccaatggcaacttgcgtctgaagtccgacccttatgcctttgaagcgcaaatgcgcccggaaaccgcgtctcttatttgcgggctgccggaaaaggttgtacagactgaagaacgcaaaaaagcgaatcagtttgatgcgccaatctctatttatgaagttcacctgggttcctggcgtcgccacaccgacaacaatttctggttgagctaccgcgagctggccgatcaactggtgccttatgctaaatggatgggctttacccacctcgaactactgcccattaacgagcatcccttcgatggcagttggggttatcagccaaccggcctgtatgcgccaacccgccgttttggtactcgcgacgacttccgttatttcattgatgccgcacacgcggctggtctgaacgtgattctcgactgggtgccaggccacttcccgactgatgactttgcgcttgccgaatttgatggcacgaacttgtatgaacacagcgatccgcgtgaaggctatcatcaggactggaacacgctgatctacaactatggtcgccgtgaagtcagtaacttcctcgtcggtaacgcgctttactggattgaacgttttggtattgatgcgctgcgcgtcgatgcggtggcgtcaatgatttatcgcgactacagccgtaaagagggggagtggattccgaacgaatttggcgggcgcgagaatcttgaagcgattgaatttttgcgtaataccaaccgtattcttggtgagcaggtttccggtgcggtgacaatggctgaggagtctaccgatttccctggcgtttctcgtccgcaggatatgggcggtctgggcttctggtacaagtggaacctcggctggatgcatgacaccctggactacatgaagctcgacccggtttatcgtcagtatcatcacgataaactgaccttcgggattctctacaactacactgaaaacttcgtcctgccgttgtcgcatgatgaagtggtccacggtaaaaaatcgattctcgaccgcatgccgggcgacgcatggcagaaattcgcgaacctgcgcgcctactatggctggatgtgggcattcccgggcaagaaactactgttcatgggtaacgaatttgcccagggccgcgagtggaaccatgacgccagcctcgactggcatctgttggaaggcggcgataactggcaccacggtgtccagcgtctggtgcgcgatctgaacctcacctaccgccaccataaagcaatgcatgaactggattttgacccgtacggctttgaatggctggtggtggatgacaaagaacgctcggtgctgatctttgtgcgtcgcgataaagagggtaacgaaatcatcgttgccagtaactttacgccggtacc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1585312_sequence/1
type: Sequence
persistent identity: BBa K1585312 sequence
display id: BBa_K1585312_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggttagtttagagaagaacgatcacttaatgttggcgcgccagctgccattgaaatctgttgccctgatactggcgggaggacgtggtacccgcctgaaggatttaaccaataagcgagcaaaaccggccgtacacttcggcggtaagttccgcattatcgactttgcgctgtctaactgcatcaactccgggatccgtcgtatgggcgtgatcacccagtaccagtcccacactctggtgcagcacattcagcgcggctggtcattcttcaatgaagaaatgaacgagtttgtcgatctgctgccagcacagcagagaatgaaaggggaaaactggtatcgcggcaccgcagatgcggtcacccaaaacctcgacattatccgccgttataaagcggaatacgtggtgatcctggcgggcgaccatatctacaagcaagactactcgcgtatgcttatcgatcacgtcgaaaaaggcgcacgttgcaccgttgcttgtatgccagtaccgattgaagaagcctccgcatttggcgttatggcggttgatgagaacgataaaattatcgaatttgttgaaaaacctgctaacccgccgtcaatgccgaacgatccgagcaaatctctggcgagtatgggtatctacgtctttgacgccgactatctgtatgaactgctggaagaagacgatcgcgatgagaactccagccacgactttggcaaagatttgattcccaagatcaccgaagccggtctggcctatgcgcacccgttcccgctctcttgcgtacaatccgacccggatgccgagccgtactggcgcgatgtgggtacgctggaagcttactggaaagcgaacctcgatctggcctctgtggtgccggaactggatatgtacgatcgcaattggccaattcgcacctacaatgaatcattaccgccagcgaaattcgtgcaggatcgctccggtagccacgggatgacccttaactcactggtttccgacggttgtgtgatctccggttcggtggtggtgcagtccgttctgttctcgcgcgttcgcgtgaactcattctgcaacattgattccgccgtattgttaccggaagtatgggtaggtcgctcgtgccgtctgcgccgctgcgtcatcgatcgtgcttgtgttattccggaaggcatggtgattggtgaaaacgcagaggaagatgcacgtcgtttctatcgttcagaagaaggcatcgtgctggtaacgcgcgaaatgctacggaagttagggcataaacaggagcgataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1585300_sequence/1
type: Sequence
persistent identity: BBa K1585300 sequence
display id: BBa_K1585300_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcaggttttacatgtatgttcagagatgttcccgctgcttaaaaccggcggtctggctgatgttattggggcattacccgcagcacaaatcgcagacggcgttgacgctcgcgtactgttgcctgcatttcccgatattcgccgtggcgtgaccgatgcccaggtagtatcccgtcgtgataccttcgccggacatatcacgctgttgttcggtcattacaacggggttggcatttacctgattgacgcgccgcatctctatgatcgtccgggaagcccgtatcacgataccaacttatttgcctataccgacaacgtattgcgttttgcgctgctggggtgggttggggcagaaatggccagcgggcttgacccattctggcgtcctgatgtggtgcatgcccacgactggcatgcaggccttgcgcctgcgtatctggcggcgcgcgggcgtccggcgaagtcggtgtttactgtgcacaacctggcctatcaaggcatgttttatgcacatcacatgaatgacatccaattgccatggtcattctttaatattcatgggctggaatttaacggacaaatctctttcctgaaggccggtctgtactatgccgatcacattacggcggtcagtccaacctacgctcgcgagatcaccgaaccgcagtttgcctacggtatggaaggtctgttgcaacagcgtcaccgtgaagggcgtctttccggcgtactgaacggcgtggacgagaaaatctggagtccagagacggacttactgttggcctcgcgttacacccgcgatacgttggaagataaagcggaaaataagcgccagttacaaatcgcaatggggcttaaggttgacgataaagtgccgctttttgcagtggtgagccgtctgaccagccagaaaggtctcgacctggtgctggaagccttaccgggtcttctggagcagggcgggcaactggcgctactcggcgcgggcgatccggtgctgcaagaaggtttccttgcggcggcagcggaataccccggtcaggtgggcgttcagattggctatcacgaagcattttcgcatcgcattatgggcggcgcggacgtcattctggtgcccagccgttttgaaccgtgcggcttaacgcaactttatggattgaagtacggtacgctgccgttagtgcggcgcaccggcgggcttgctgatacggtttctgactgttctcttgagaaccttgcagatggcgtcgccagtgggtttgtctttgaagatagtaatgcctggtcgctgttacgggctattcgacgtgcttttgtactgtggtcccgtccttcactgtggcggtttgtgcaacgtcaggctatggcaatggattttagctggcaggtcgcggcgaagtcgtaccgtgagctttactatcgcttgaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1585301_sequence/1
type: Sequence
persistent identity: BBa K1585301 sequence
display id: BBa_K1585301_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccgatcgtatcgatagagacgtgattaacgcgctaattgcaggccattttgccgatcctttttccgtactgggaatgcataaaaccaccgcgggactggaagtccgtgcccttttacccgacgctaccgatgtgtgggtgattgaaccgaaaaccgggcgcaaactcgcaaaactggagtgtctcgactcacggggattctttagcggcgtcattccgcgacgtaagaattttttccgctatcagttggctgttgtctggcatggtcagcaaaacctgattgatgatccttaccgttttggtccgctaatccaggaaatggatgcctggctattatctgaaggtactcacctgcgcccgtatgaaaccttaggcgcgcatgcagatactatggatggcgtcacaggtacgcgtttctctgtctgggctccaaacgcccgtcgggtctcggtggttgggcaattcaactactgggacggtcgccgtcacccgatgcgcctgcgtaaagagagcggcatctgggaactgtttatccctggggcgcataacggtcagctctataaatacgagatgattgatgccaatggcaacttgcgtctgaagtccgacccttatgcctttgaagcgcaaatgcgcccggaaaccgcgtctcttatttgcgggctgccggaaaaggttgtacagactgaagaacgcaaaaaagcgaatcagtttgatgcgccaatctctatttatgaagttcacctgggttcctggcgtcgccacaccgacaacaatttctggttgagctaccgcgagctggccgatcaactggtgccttatgctaaatggatgggctttacccacctcgaactactgcccattaacgagcatcccttcgatggcagttggggttatcagccaaccggcctgtatgcgccaacccgccgttttggtactcgcgacgacttccgttatttcattgatgccgcacacgcggctggtctgaacgtgattctcgactgggtgccaggccacttcccgactgatgactttgcgcttgccgaatttgatggcacgaacttgtatgaacacagcgatccgcgtgaaggctatcatcaggactggaacacgctgatctacaactatggtcgccgtgaagtcagtaacttcctcgtcggtaacgcgctttactggattgaacgttttggtattgatgcgctgcgcgtcgatgcggtggcgtcaatgatttatcgcgactacagccgtaaagagggggagtggattccgaacgaatttggcgggcgcgagaatcttgaagcgattgaatttttgcgtaataccaaccgtattcttggtgagcaggtttccggtgcggtgacaatggctgaggagtctaccgatttccctggcgtttctcgtccgcaggatatgggcggtctgggcttctggtacaagtggaacctcggctggatgcatgacaccctggactacatgaagctcgacccggtttatcgtcagtatcatcacgataaactgaccttcgggattctctacaactacactgaaaacttcgtcctgccgttgtcgcatgatgaagtggtccacggtaaaaaatcgattctcgaccgcatgccgggcgacgcatggcagaaattcgcgaacctgcgcgcctactatggctggatgtgggcattcccgggcaagaaactactgttcatgggtaacgaatttgcccagggccgcgagtggaaccatgacgccagcctcgactggcatctgttggaaggcggcgataactggcaccacggtgtccagcgtctggtgcgcgatctgaacctcacctaccgccaccataaagcaatgcatgaactggattttgacccgtacggctttgaatggctggtggtggatgacaaagaacgctcggtgctgatctttgtgcgtcgcgataaagagggtaacgaaatcatcgttgccagtaactttacgccggtaccgcgtcatgattatcgctt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118016_sequence/1
type: Sequence
persistent identity: BBa K118016 sequence
display id: BBa_K118016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggttagtttagagaagaacgatcacttaatgttggcgcgccagctgccattgaaatctgttgccctgatactggcgggaggacgtggtacccgcctgaaggatttaaccaataagcgagcaaaaccggccgtacacttcggcggtaagttccgcattatcgactttgcgctgtctaactgcatcaactccgggatccgtcgtatgggcgtgatcacccagtaccagtcccacactctggtgcagcacattcagcgcggctggtcattcttcaatgaagaaatgaacgagtttgtcgatctgctgccagcacagcagagaatgaaaggggaaaactggtatcgcggcaccgcagatgcggtcacccaaaacctcgacattatccgccgttataaagcggaatacgtggtgatcctggcgggcgaccatatctacaagcaagactactcgcgtatgcttatcgatcacgtcgaaaaaggcgcacgttgcaccgttgcttgtatgccagtaccgattgaagaagcctccgcatttggcgttatggcggttgatgagaacgataaaattatcgaatttgttgaaaaacctgctaacccgccgtcaatgccgaacgatccgagcaaatctctggcgagtatgggtatctacgtctttgacgccgactatctgtatgaactgctggaagaagacgatcgcgatgagaactccagccacgactttggcaaagatttgattcccaagatcaccgaagccggtctggcctatgcgcacccgttcccgctctcttgcgtacaatccgacccggatgccgagccgtactggcgcgatgtgggtacgctggaagcttactggaaagcgaacctcgatctggcctctgtggtgccggaactggatatgtacgatcgcaattggccaattcgcacctacaatgaatcattaccgccagcgaaattcgtgcaggatcgctccggtagccacgggatgacccttaactcactggtttccgacggttgtgtgatctccggttcggtggtggtgcagtccgttctgttctcgcgcgttcgcgtgaactcattctgcaacattgattccgccgtattgttaccggaagtatgggtaggtcgctcgtgccgtctgcgccgctgcgtcatcgatcgtgcttgtgttattccggaaggcatggtgattggtgaaaacgcagaggaagatgcacgtcgtttctatcgttcagaagaaggcatcgtgctggtaacgcgcgaaatgctacggaagttagggcataaacaggagcgataataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z