LOCUS       BBa_K1585350            1036 bp    DNA     linear       22-Nov-2024
DEFINITION  glgX targeting plasmid
ACCESSION   BBa_K1585350
VERSION     BBa_K1585350.1  
FEATURES             Location/Qualifiers
     promoter        29..57
                     /label=J23119
     misc_feature    64..83
                     /label=N20 for glgX targeting
     misc_feature    84..125
                     /label=cas9BIRE
     misc_feature    126..165
                     /label=spy-tem
     misc_feature    191..590
                     /label=left homology arm
     CDS             591..623
                     /label=iGEM AACHEN MULTI STOP
     misc_feature    595..597
                     /label=stop
     misc_feature    609..611
                     /label=stop
     misc_feature    620..622
                     /label=stop
     misc_feature    624..1008
                     /label=right homology arm
     CDS             191..638
                     /label=beginning of glgX cds
     CDS             635..1008
                     /label=end of glgB cds
ORIGIN
        1 ggtatttcac accgcatatg ctggatcctt gacagctagc tcagtcctag gtataatact
       61 agtgtaatgc gcgccgaggg gaggttttag agctagaaat agcaagttaa aataaggcta
      121 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttgaat tctctagagt
      181 cgacctgcag gcggggcatc atcttcccag tcatagtgat caaccaccac tacgcatttc
      241 ggcgcaatgg cggcgttgtc gcgatagtca ggttcattat gaccggcgtg cagcagcggg
      301 ttatctttaa actccccgtc aatttgccgc gcgcaaggat caatcaacaa cttcgccggg
      361 ttaaagcgat gcccctcggc gggttgccag gggccatgaa cgcgataacc ataacgcaaa
      421 cccgggcgcg catccggcag ataaccgtgc caaatgtcgc cactgtgccc tggcaagtca
      481 tagcgatgtt cctggccatt ggcgtcaaag acacacagtt ctacccgctc ggcatgagcg
      541 gagaaaagtg tgaagttgac gccctgaccg tcgtaatgcg cgccgagggg gttttcatgg
      601 cacgccgctt acatttcgcc tatggcgagt tgtgtcattc tgcctcccga accagccaga
      661 tagtggccag cggtggtagc gtcaggctta gtgaatgctg acgaccgtgg ctggcaatct
      721 catcgctgtg taccgtgccg ccattgcctg cattactgcc gtgatagtgc atggaatcgg
      781 tattgaggat ttcacgccat ttgcccggct ggtttatgcc gaagcgataa tcatgacgcg
      841 gtaccggcgt aaagttactg gcaacgatga tttcgttacc ctctttatcg cgacgcacaa
      901 agatcagcac cgagcgttct ttgtcatcca ccaccagcca ttcaaagccg tacgggtcaa
      961 aatccagttc atgcattgct ttatggtggc ggtaggtgag gttcagataa gcttagatct
     1021 attaccctgt tatccc
//