BBa_K1585350 1 anti X glgX targeting plasmid 2015-09-04T11:00:00Z 2015-09-18T06:05:04Z The part consists of genomic repair templates with a gene-edit and a synthetic sgRNA sequence. This plasmid is targeting glgX in the E. coli genome by expressing a sgRNA. It contains a repair template that disrupts the beginning of the glgX coding sequence and introduces a stop codon. false false _2002_ 24505 19522 9 true It is not designed to be BioBrick-compatible, but it can be used AS IS to knock out glgX in E. coli. It has to be used together with pCas (https://www.addgene.org/62225/) false Laura Helleckes, Michael Osthege, Sarah Lubjuhn annotation2444220 1 right homology arm range2444220 1 624 1008 annotation2444212 1 N20 for glgX targeting range2444212 1 64 83 annotation2444221 1 beginning of glgX cds range2444221 1 191 638 annotation2444217 1 stop range2444217 1 595 597 annotation2444214 1 spy-tem range2444214 1 126 165 annotation2444215 1 left homology arm range2444215 1 191 590 annotation2444216 1 iGEM AACHEN MULTI STOP range2444216 1 591 623 annotation2444213 1 cas9BIRE range2444213 1 84 125 annotation2444218 1 stop range2444218 1 609 611 annotation2444219 1 stop range2444219 1 620 622 annotation2444222 1 end of glgB cds range2444222 1 635 1008 annotation2444211 1 J23119 range2444211 1 29 57 BBa_K1585350_sequence 1 ggtatttcacaccgcatatgctggatccttgacagctagctcagtcctaggtataatactagtgtaatgcgcgccgaggggaggttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgctttttttgaattctctagagtcgacctgcaggcggggcatcatcttcccagtcatagtgatcaaccaccactacgcatttcggcgcaatggcggcgttgtcgcgatagtcaggttcattatgaccggcgtgcagcagcgggttatctttaaactccccgtcaatttgccgcgcgcaaggatcaatcaacaacttcgccgggttaaagcgatgcccctcggcgggttgccaggggccatgaacgcgataaccataacgcaaacccgggcgcgcatccggcagataaccgtgccaaatgtcgccactgtgccctggcaagtcatagcgatgttcctggccattggcgtcaaagacacacagttctacccgctcggcatgagcggagaaaagtgtgaagttgacgccctgaccgtcgtaatgcgcgccgagggggttttcatggcacgccgcttacatttcgcctatggcgagttgtgtcattctgcctcccgaaccagccagatagtggccagcggtggtagcgtcaggcttagtgaatgctgacgaccgtggctggcaatctcatcgctgtgtaccgtgccgccattgcctgcattactgccgtgatagtgcatggaatcggtattgaggatttcacgccatttgcccggctggtttatgccgaagcgataatcatgacgcggtaccggcgtaaagttactggcaacgatgatttcgttaccctctttatcgcgacgcacaaagatcagcaccgagcgttctttgtcatccaccaccagccattcaaagccgtacgggtcaaaatccagttcatgcattgctttatggtggcggtaggtgaggttcagataagcttagatctattaccctgttatccc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z