LOCUS       BBa_K1585351            1259 bp    DNA     linear       22-May-2024
DEFINITION  glgP targeting plasmid
ACCESSION   BBa_K1585351
VERSION     BBa_K1585351.1  
FEATURES             Location/Qualifiers
     misc_feature    30..69
                     /label=spy-tem
     misc_feature    70..111
                     /label=cas9BIRE
     misc_feature    112..131
                     /label=N20 for glgP targeting left
     promoter        138..166
                     /label=pJ23119
     promoter        237..265
                     /label=pJ23119
     misc_feature    272..291
                     /label=N20 for glgP targeting right
     misc_feature    292..333
                     /label=cas9BIRE
     misc_feature    334..373
                     /label=spy-tem
     misc_feature    399..798
                     /label=left homology arm 
     misc_feature    832..1231
                     /label=right homolgy arm
     CDS             799..831
                     /label=iGEM AACHEN MULTI STOP
     CDS             721..798
                     /label=end of glgP cds
     CDS             851..1231
                     /label=glgA cds
     misc_feature    803..805
                     /label=stop
     misc_feature    817..819
                     /label=stop
     misc_feature    828..830
                     /label=stop
ORIGIN
        1 ggtatttcac accgcatatg ctggatccaa aaaaagcacc gactcggtgc cactttttca
       61 agttgataac ggactagcct tattttaact tgctatttct agctctaaaa catatgggct
      121 acttctcttc tactagtatt atacctagga ctgagctagc tgtcaaaggt tctgttaagt
      181 aactgaaccc aatgtcgtta gtgacgctta cctcttaaga ggtcactgac ctaacattga
      241 cagctagctc agtcctaggt ataatactag ttgtaaacgg agcattcata tgttttagag
      301 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
      361 tcggtgcttt ttttgaattc tctagagtcg acctgcagaa tgcgcccagc gccagcataa
      421 aaattttgag cagaaggtaa attgttggcg tgataaggat attacaaact tcaataatac
      481 cggcaagtcc gacacccagc atggcaataa ccaccgccaa aaattgcgcc agtatgggga
      541 tgccgaaaaa agtcattacc agcgaggtca aaatccattt tctgttttgc attattcttt
      601 ccattctttt tgaatggtga aattatactc cccgagtccc cttgcccctt ctggacactt
      661 ttccgaaatg atggcggaaa aaaacgggac cctttggccc cgttctattt attggtgaac
      721 ttacaatctc accggatcga tatgccagat atgatcggcg tactctttga tagtacggtc
      781 agaagagaag tagcccatgt tttcatggca cgccgcttac atttcgccta ttataggcgt
      841 ttcctgaaaa ctatttcaag cgatagtaaa gctcacggta cgacttcgcc gcgacctgcc
      901 agctaaaatc cattgccata gcctgacgtt gcacaaaccg ccacagtgaa ggacgggacc
      961 acagtacaaa agcacgtcga atagcccgta acagcgacca ggcattacta tcttcaaaga
     1021 caaacccact ggcgacgcca tctgcaaggt tctcaagaga acagtcagaa accgtatcag
     1081 caagcccacc ggtgcgccgc actaacggca gcgtaccgta cttcaatcca taaagttgcg
     1141 ttaagccgca cggttcaaaa cggctgggca ccagaatgac gtccgcgccg cccataatgc
     1201 gatgcgaaaa tgcttcgtga tagccaatct gaagcttaga tctattaccc tgttatccc
//