BBa_K1585351 1 BBa_K1585351 glgP targeting plasmid 2015-09-05T11:00:00Z 2015-09-18T06:06:01Z The part consists of genomic repair templates with a gene-edit and a synthetic sgRNA sequence. This plasmid is targeting glgP in the E. coli genome by expressing two sgRNAs. The complete glgP coding sequence is cut out and a stop codon is introduced by the repair templates. false false _2002_ 24505 24589 9 true It is not designed to be BioBrick-compatible, but it can be used AS IS to knock out glgX in E. coli. It has to be used together with pCas (https://www.addgene.org/62225/) false Michael Osthege, Sarah Lubjuhn, Laura Helleckes annotation2445182 1 stop range2445182 1 828 830 annotation2445173 1 cas9BIRE range2445173 1 292 333 annotation2445168 1 cas9BIRE range2445168 1 70 111 annotation2445178 1 end of glgP cds range2445178 1 721 798 annotation2445169 1 N20 for glgP targeting left range2445169 1 112 131 annotation2445179 1 glgA cds range2445179 1 851 1231 annotation2445172 1 N20 for glgP targeting right range2445172 1 272 291 annotation2445176 1 right homolgy arm range2445176 1 832 1231 annotation2445170 1 pJ23119 range2445170 1 138 166 annotation2445171 1 pJ23119 range2445171 1 237 265 annotation2445174 1 spy-tem range2445174 1 334 373 annotation2445175 1 left homology arm range2445175 1 399 798 annotation2445177 1 iGEM AACHEN MULTI STOP range2445177 1 799 831 annotation2445180 1 stop range2445180 1 803 805 annotation2445181 1 stop range2445181 1 817 819 annotation2445167 1 spy-tem range2445167 1 30 69 BBa_K1585351_sequence 1 ggtatttcacaccgcatatgctggatccaaaaaaagcaccgactcggtgccactttttcaagttgataacggactagccttattttaacttgctatttctagctctaaaacatatgggctacttctcttctactagtattatacctaggactgagctagctgtcaaaggttctgttaagtaactgaacccaatgtcgttagtgacgcttacctcttaagaggtcactgacctaacattgacagctagctcagtcctaggtataatactagttgtaaacggagcattcatatgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgctttttttgaattctctagagtcgacctgcagaatgcgcccagcgccagcataaaaattttgagcagaaggtaaattgttggcgtgataaggatattacaaacttcaataataccggcaagtccgacacccagcatggcaataaccaccgccaaaaattgcgccagtatggggatgccgaaaaaagtcattaccagcgaggtcaaaatccattttctgttttgcattattctttccattctttttgaatggtgaaattatactccccgagtccccttgccccttctggacacttttccgaaatgatggcggaaaaaaacgggaccctttggccccgttctatttattggtgaacttacaatctcaccggatcgatatgccagatatgatcggcgtactctttgatagtacggtcagaagagaagtagcccatgttttcatggcacgccgcttacatttcgcctattataggcgtttcctgaaaactatttcaagcgatagtaaagctcacggtacgacttcgccgcgacctgccagctaaaatccattgccatagcctgacgttgcacaaaccgccacagtgaaggacgggaccacagtacaaaagcacgtcgaatagcccgtaacagcgaccaggcattactatcttcaaagacaaacccactggcgacgccatctgcaaggttctcaagagaacagtcagaaaccgtatcagcaagcccaccggtgcgccgcactaacggcagcgtaccgtacttcaatccataaagttgcgttaagccgcacggttcaaaacggctgggcaccagaatgacgtccgcgccgcccataatgcgatgcgaaaatgcttcgtgatagccaatctgaagcttagatctattaccctgttatccc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z