##gff-version 3
##sequence-region BBa_K1585351 30 1231
BBa_K1585351	.	sequence_feature	30	69	.	+	0	ID=annotation2445167;Name=spy-tem
BBa_K1585351	.	stop_codon	828	830	.	+	0	ID=annotation2445182;Name=stop
BBa_K1585351	.	CDS	851	1231	.	+	0	ID=annotation2445179;Name=glgA cds
BBa_K1585351	.	sequence_feature	292	333	.	+	0	ID=annotation2445173;Name=cas9BIRE
BBa_K1585351	.	non_covalent_binding_site	112	131	.	+	0	ID=annotation2445169;Name=N20 for glgP targeting left
BBa_K1585351	.	CDS	721	798	.	+	0	ID=annotation2445178;Name=end of glgP cds
BBa_K1585351	.	sequence_feature	70	111	.	+	0	ID=annotation2445168;Name=cas9BIRE
BBa_K1585351	.	promoter	138	166	.	+	0	ID=annotation2445170;Name=pJ23119
BBa_K1585351	.	sequence_feature	832	1231	.	+	0	ID=annotation2445176;Name=right homolgy arm
BBa_K1585351	.	non_covalent_binding_site	272	291	.	+	0	ID=annotation2445172;Name=N20 for glgP targeting right
BBa_K1585351	.	promoter	237	265	.	+	0	ID=annotation2445171;Name=pJ23119
BBa_K1585351	.	sequence_feature	334	373	.	+	0	ID=annotation2445174;Name=spy-tem
BBa_K1585351	.	CDS	799	831	.	+	0	ID=annotation2445177;Name=iGEM AACHEN MULTI STOP
BBa_K1585351	.	sequence_feature	399	798	.	+	0	ID=annotation2445175;Name=left homology arm 
BBa_K1585351	.	stop_codon	817	819	.	+	0	ID=annotation2445181;Name=stop
BBa_K1585351	.	stop_codon	803	805	.	+	0	ID=annotation2445180;Name=stop
>BBa_K1585351
ggtatttcacaccgcatatgctggatccaaaaaaagcaccgactcggtgccactttttcaagttgataacggactagcc
ttattttaacttgctatttctagctctaaaacatatgggctacttctcttctactagtattatacctaggactgagcta
gctgtcaaaggttctgttaagtaactgaacccaatgtcgttagtgacgcttacctcttaagaggtcactgacctaacat
tgacagctagctcagtcctaggtataatactagttgtaaacggagcattcatatgttttagagctagaaatagcaagtt
aaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgctttttttgaattctctagagtcgacctg
cagaatgcgcccagcgccagcataaaaattttgagcagaaggtaaattgttggcgtgataaggatattacaaacttcaa
taataccggcaagtccgacacccagcatggcaataaccaccgccaaaaattgcgccagtatggggatgccgaaaaaagt
cattaccagcgaggtcaaaatccattttctgttttgcattattctttccattctttttgaatggtgaaattatactccc
cgagtccccttgccccttctggacacttttccgaaatgatggcggaaaaaaacgggaccctttggccccgttctattta
ttggtgaacttacaatctcaccggatcgatatgccagatatgatcggcgtactctttgatagtacggtcagaagagaag
tagcccatgttttcatggcacgccgcttacatttcgcctattataggcgtttcctgaaaactatttcaagcgatagtaa
agctcacggtacgacttcgccgcgacctgccagctaaaatccattgccatagcctgacgttgcacaaaccgccacagtg
aaggacgggaccacagtacaaaagcacgtcgaatagcccgtaacagcgaccaggcattactatcttcaaagacaaaccc
actggcgacgccatctgcaaggttctcaagagaacagtcagaaaccgtatcagcaagcccaccggtgcgccgcactaac
ggcagcgtaccgtacttcaatccataaagttgcgttaagccgcacggttcaaaacggctgggcaccagaatgacgtccg
cgccgcccataatgcgatgcgaaaatgcttcgtgatagccaatctgaagcttagatctattaccctgttatccc