BBa_K1585351
1
BBa_K1585351
glgP targeting plasmid
2015-09-05T11:00:00Z
2015-09-18T06:06:01Z
The part consists of genomic repair templates with a gene-edit and a synthetic sgRNA sequence.
This plasmid is targeting glgP in the E. coli genome by expressing two sgRNAs. The complete glgP coding sequence is cut out and a stop codon is introduced by the repair templates.
false
false
_2002_
24505
24589
9
true
It is not designed to be BioBrick-compatible, but it can be used AS IS to knock out glgX in E. coli.
It has to be used together with pCas (https://www.addgene.org/62225/)
false
Michael Osthege, Sarah Lubjuhn, Laura Helleckes
annotation2445169
1
N20 for glgP targeting left
range2445169
1
112
131
annotation2445173
1
cas9BIRE
range2445173
1
292
333
annotation2445181
1
stop
range2445181
1
817
819
annotation2445167
1
spy-tem
range2445167
1
30
69
annotation2445172
1
N20 for glgP targeting right
range2445172
1
272
291
annotation2445168
1
cas9BIRE
range2445168
1
70
111
annotation2445177
1
iGEM AACHEN MULTI STOP
range2445177
1
799
831
annotation2445170
1
pJ23119
range2445170
1
138
166
annotation2445180
1
stop
range2445180
1
803
805
annotation2445178
1
end of glgP cds
range2445178
1
721
798
annotation2445171
1
pJ23119
range2445171
1
237
265
annotation2445179
1
glgA cds
range2445179
1
851
1231
annotation2445182
1
stop
range2445182
1
828
830
annotation2445174
1
spy-tem
range2445174
1
334
373
annotation2445176
1
right homolgy arm
range2445176
1
832
1231
annotation2445175
1
left homology arm
range2445175
1
399
798
BBa_K1585351_sequence
1
ggtatttcacaccgcatatgctggatccaaaaaaagcaccgactcggtgccactttttcaagttgataacggactagccttattttaacttgctatttctagctctaaaacatatgggctacttctcttctactagtattatacctaggactgagctagctgtcaaaggttctgttaagtaactgaacccaatgtcgttagtgacgcttacctcttaagaggtcactgacctaacattgacagctagctcagtcctaggtataatactagttgtaaacggagcattcatatgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgctttttttgaattctctagagtcgacctgcagaatgcgcccagcgccagcataaaaattttgagcagaaggtaaattgttggcgtgataaggatattacaaacttcaataataccggcaagtccgacacccagcatggcaataaccaccgccaaaaattgcgccagtatggggatgccgaaaaaagtcattaccagcgaggtcaaaatccattttctgttttgcattattctttccattctttttgaatggtgaaattatactccccgagtccccttgccccttctggacacttttccgaaatgatggcggaaaaaaacgggaccctttggccccgttctatttattggtgaacttacaatctcaccggatcgatatgccagatatgatcggcgtactctttgatagtacggtcagaagagaagtagcccatgttttcatggcacgccgcttacatttcgcctattataggcgtttcctgaaaactatttcaagcgatagtaaagctcacggtacgacttcgccgcgacctgccagctaaaatccattgccatagcctgacgttgcacaaaccgccacagtgaaggacgggaccacagtacaaaagcacgtcgaatagcccgtaacagcgaccaggcattactatcttcaaagacaaacccactggcgacgccatctgcaaggttctcaagagaacagtcagaaaccgtatcagcaagcccaccggtgcgccgcactaacggcagcgtaccgtacttcaatccataaagttgcgttaagccgcacggttcaaaacggctgggcaccagaatgacgtccgcgccgcccataatgcgatgcgaaaatgcttcgtgatagccaatctgaagcttagatctattaccctgttatccc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z