LOCUS       BBa_K1586000            1021 bp    DNA     linear       14-Nov-2024
DEFINITION  Synthetic toehold riboswitch - J23100
ACCESSION   BBa_K1586000
VERSION     BBa_K1586000.1  
FEATURES             Location/Qualifiers
     promoter        1..33
                     /label=J23100
     misc_feature    34..36
                     /label=GGG leader sequence
     misc_feature    37..61
                     /label=Switch region
     CDS             88..884
                     /label=Protein coding region
     misc_feature    893..1021
                     /label=Terminator
     CDS             126..884
                     /label=GFP
     stem_loop       51..104
                     /label=Stem-loop
     misc_feature    93..95
                     /label=ATG
     misc_feature    879..884
                     /label=Double stop codon (TAA TAA)
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagcgggtc ttatcttatc tatctcgttt
       61 atccctgcat acagaaacag aggagatatg caatgataaa cgagaacctg gcggcagcgc
      121 aaaagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
      181 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
      241 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccgtggccaa
      301 cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca gatcacatga
      361 aacagcatga ctttttcaag agtgccatgc ccgaaggtta cgtacaggaa agaactatat
      421 ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc
      481 ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac
      541 acaaattgga atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga
      601 atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc gttcaactag
      661 cagaccatta tcaacaaaat actccgattg gcgatggccc tgtcctttta ccagacaacc
      721 attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg
      781 tccttcttga gtttgtaacc gctgctggga ttacacatgg catggatgaa ctatacaaaa
      841 ggcctacagc aaacgacgaa aactacgctg catcagttta ataaatttaa atccaggcat
      901 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
      961 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1021 a
//