LOCUS       BBa_K1586001             921 bp    DNA     linear       22-Nov-2024
DEFINITION  Synthetic toehold riboswitch - T7
ACCESSION   BBa_K1586001
VERSION     BBa_K1586001.1  
FEATURES             Location/Qualifiers
     promoter        1..17
                     /label=T7
     misc_feature    18..20
                     /label=GGG leader sequence
     misc_feature    21..47
                     /label=Switch region
     CDS             75..866
                     /label=Translated region
     CDS             108..866
                     /label=GFP
     misc_feature    867..921
                     /label=T7 terminator
     stem_loop       33..81
                     /label=Stem-loop
ORIGIN
        1 taatacgact cactataggg tcttatctta tctatctcgt ttatccctgc atacagaaac
       61 agaggagata tgcaatgata aacgagaacc tggcggcagc gcaaaagatg cgtaaaggag
      121 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
      181 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
      241 aatttatttg cactactgga aaactacctg ttccgtggcc aacacttgtc actactttcg
      301 gttatggtgt tcaatgcttt gcgagatacc cagatcacat gaaacagcat gactttttca
      361 agagtgccat gcccgaaggt tacgtacagg aaagaactat atttttcaaa gatgacggga
      421 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
      481 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
      541 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact
      601 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
      661 atactccgat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
      721 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
      781 ccgctgctgg gattacacat ggcatggatg aactatacaa aaggcctaca gcaaacgacg
      841 aaaactacgc tgcatcagtt taataaattt aaattagcat aaccccttgg ggcctctaaa
      901 cgggtcttga ggggtttttt g
//