LOCUS       BBa_K1586002            1006 bp    DNA     linear       23-Dec-2024
DEFINITION  EsxB synthetic toehold switch J23100 STOP
ACCESSION   BBa_K1586002
VERSION     BBa_K1586002.1  
FEATURES             Location/Qualifiers
     promoter        1..33
                     /label=J23100
     misc_feature    34..36
                     /label=GGG leader sequence
     misc_feature    37..61
                     /label=Switch region
     RBS             87..98
                     /label=B0034
     CDS             102..869
                     /label=Protein coding region
     CDS             150..869
                     /label=GFP (E0040)
     misc_feature    878..1006
                     /label=Terminator
     misc_feature    114..116
                     /label=TAA
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagcggggg accccggcct gacgaatata
       61 agtcgtttaa aaaaaaaaat ataattaaag aggagaaacg aatgatattc gtctaaagtg
      121 gtagtggtag tggtgaatgc gaatgcataa tgcgtaaagg agaagaactt ttcactggag
      181 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
      241 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
      301 gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct
      361 ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag
      421 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg
      481 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta
      541 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat
      601 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca
      661 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
      721 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc
      781 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac
      841 atggcatgga tgaactatac aaataataaa tttaaatcca ggcatcaaat aaaacgaaag
      901 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      961 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//