BBa_K1587004 1 BBa_K1587004 Butyrate synthesis pathway regulated by constitutive promoter p(Bla) 2015-09-13T11:00:00Z 2015-09-16T11:58:25Z tesB, ccr, crt, and hbd sequence are from Clostridium acetobutylicum. atoB sequence is from E.coli. The strong RBS and Terminator were used according to respectively BBa_B0030 and BBa_B1006 Constitutive promoter p(Bla). Crotonyl-coA reductase ccr coding region. 3-hydroxybutyryl-CoA dehydrogenase hbd coding region. 3-hydroxybutyryl-CoA dehydratase crt coding region. Acyl-CoA thioesterase 2 tesB coding region. acetyl-CoA acetyltransferase atoB coding region. Every genes are framed by strong RBS based on Ron Weiss thesis. This operon ends with an artificial terminator according to BBa_B1006. Due to a lack of time we decided to have this construction synthesised instead of using the assembly protocol. Unfotunatly NMR analysis did not yield a significant over production of butyrate in E. coli. false false _2004_ 24598 24598 9 false p(Bla) is followed by NheI restriction site and ccr is followed by XhoI restriction site false Alexandre Le Scornet annotation2461494 1 rbs range2461494 1 42 56 annotation2461504 1 Terminator range2461504 1 5154 5192 annotation2461496 1 rbs range2461496 1 1413 1427 annotation2461493 1 p(Bla) range2461493 1 1 35 annotation2461503 1 atoB range2461503 1 3969 5153 annotation2461499 1 crt range2461499 1 2292 3077 annotation2461502 1 rbs range2461502 1 3954 3968 annotation2461498 1 rbs range2461498 1 2277 2291 annotation2461500 1 rbs range2461500 1 3078 3092 annotation2461495 1 ccr range2461495 1 57 1406 annotation2461501 1 tesB range2461501 1 3093 3953 annotation2461497 1 hbd range2461497 1 1428 2276 BBa_K1587004_sequence 1 tgtaagtttatacataggcgagtactctgttatgggctagcattaaagaggagaaaatggttactgtaaaagacatcctggacgctatccagtctaaggacgcgacctccgctgacttcgcggctcttcagctcccggaaagctaccgtgcaatcactgttcacaaagatgaaaccgaaatgtttgctggcctggagactcgcgataaagacccacgtaaatctatccatctggacgaagttcctgtaccagaactgggcccgggtgaagctctggttgctgttatggcatcatctgttaactataactccgtttggacctctattttcgaaccggtatccactttcgctttcctggaacgttacggcaaactgtccccgctgaccaagcgccacgacctcccgtaccacatcatcggctctgatctggcaggcgttgttctgcgtactggtccgggtgttaacgcttggcagccgggcgacgaggtagttgctcactgcctgtctgttgaactggagtccccagacggtcacgacgataccatgctggacccggaacagcgtatctggggcttcgaaactaacttcggtggcctggctgaaatcgctctggtaaaaactaaccagctgatgccgaaaccgaaacatttgacctgggaagaggcagctgcgccgggtctggtaaactctactgcataccgtcagctggtttcccgtaacggcgctgctatgaaacagggtgacaacgttctgatctggggcgcgtctggcggtttgggttcttatgctacccagttcgctctggctggcggtgccaacccgatctgcgttgtctccagcccgcagaaagctgaaatctgccgttctatgggtgctgaagcaatcattgatcgtaacgcagaaggttacaaattctggaaagacgaacacacccaggacccgaaagagtggaaacgttttggcaaacgtatccgtgaactgactggcggcgaagacatcgacatcgttttcgaacacccgggccgtgaaaccttcggtgcttccgtatacgttactcgtaagggtggtaccatcactacctgtgcatccacctccggctacatgcatgaatatgacaaccgttacctgtggatgagcctgaaacgcatcatcggctcccacttcgctaactaccgtgaggcttatgaagctaaccgcctgattgctaagggcaaaatccatccaactctgagcaaaacctattctctggaagaaaccggtcaggcggcttatgacgttcaccgcaacctgcatcagggtaaagtaggtgttctgtgtctggctccggaagagggtctgggcgtacgcgacgcagaaatgcgtgctcagcacatcgacgcgattaaccgtttccgcaacgtgtcatgactcgagattaaagaggagaaaatgaagaaagtttgtgttattggcgctggtactatgggctccggtatcgctcaggcattcgctgctaagggcttcgaagtagttctgcgtgatatcaaagacgagtttgtggaccgtggcctggacttcatcaacaaaaacctgtccaagctggttaaaaagggtaagatcgaagaagctaccaaagttgaaatcctgactcgtatctctggcaccgtagacctgaacatggctgcggactgcgacctggttattgaagctgctgtggagcgcatggatattaaaaagcagatctttgcagacctggacaacatttgcaaaccggagaccatcctggcatccaacacttcttccctgagcatcacagaagttgcttcagctactaaacgtccggacaaagttatcggcatgcatttcttcaacccggctccggtaatgaaactggttgaagttatccgtggtattgctacttcccaggaaactttcgacgctgttaaagaaactagcatcgcgatcggcaaagatccggttgaagttgcagaagctccgggtttcgtagttaaccgtattctgatcccgatgatcaacgaagcagttggcatcctggctgaaggtattgcatctgttgaagacatcgacaaagctatgaaactcggcgcgaaccatccgatgggtccgctggaactgggtgacttcatcggtctggacatctgcctggcgatcatggatgtcctgtactccgagaccggcgactctaaataccgcccgcacactctgctgaaaaaatacgtacgcgcaggttggctgggccgtaagtctggtaaaggcttctacgactactctaagtaaattaaagaggagaaaatggaactgaacaatgttatcttagaaaaggaaggcaaagtagctgtggttaccatcaaccgcccgaaagcactgaacgccctgaactctgacaccctgaaagaaatggactacgtgatcggtgaaattgagaacgactctgaagtactggcagtgattctgaccggcgctggtgaaaaaagcttcgtagctggcgctgacatctccgaaatgaaagagatgaacactatcgaaggtcgtaaatttggcatcctgggtaacaaagtgttccgtcgtctggaactgctggaaaagccggttatcgccgcagttaacggcttcgctctgggtggcggctgcgaaatcgcgatgtcctgcgacattcgcatcgcttcctctaacgcacgtttcggtcaaccggaagtaggtctgggcatcaccccgggcttcggtggcacccagcgtctgtcccgtctggttggtatgggcatggctaaacagctgatcttcaccgcgcagaacatcaaggcagacgaagctctgcgtatcggcctggttaacaaagtagttgaaccgtctgaactgatgaacactgctaaagaaatcgctaacaaaatcgtatctaacgcaccggtagcagttaaactgagcaaacaggctatcaaccgtggcatgcagtgcgacatcgacaccgctctggcttttgagtccgaagcgtttggtgaatgcttcagcactgaagaccagaaagacgcgatgactgctttcatcgaaaagcgcaaaatcgaaggcttcaaaaaccgctaaattaaagaggagaaaatgagtcaggcgctaaaaaatttactgacattgttaaatctggaaaaaattgaggaaggactctttcgcggccagagtgaagatttaggtttacgccaggtgtttggcggccaggtcgtgggtcaggccttgtatgctgcaaaagagaccgtccctgaagagcggctggtacattcgtttcacagctactttcttcgccctggcgatagtaagaagccgattatttatgatgtcgaaacgctgcgtgacggtaacagcttcagcgcccgccgggttgctgctattcaaaacggcaaaccgattttttatatgactgcctctttccaggcaccagaagcgggtttcgaacatcaaaaaacaatgccgtccgcgccagcgcctgatggcctcccttcggaaacgcaaatcgcccaatcgctggcgcacctgctgccgccagtgctgaaagataaattcatctgcgatcgtccgctggaagtccgtccggtggagtttcataacccactgaaaggtcacgtcgcagaaccacatcgtcaggtgtggatccgcgcaaatggtagcgtgccggatgacctgcgcgttcatcagtatctgctcggttacgcttctgatcttaacttcctgccggtagctctacagccgcacggcatcggttttctcgaaccggggattcagattgccaccattgaccattccatgtggttccatcgcccgtttaatttgaatgaatggctgctgtatagcgtggagagcacctcggcgtccagcgcacgtggctttgtgcgcggtgagttttatacccaagacggcgtactggttgcctcgaccgttcaggaaggggtgatgcgtaatcacaattaaattaaagaggagaaaatgaaaaattgtgtcatcgtcagtgcggtacgtactgctatcggtagttttaacggttcactcgcttccaccagcgccatcgacctgggggcgacagtaattaaagccgccattgaacgtgcaaaaatcgattcacaacacgttgatgaagtgattatgggtaacgtgttacaagccgggctggggcaaaatccggcgcgtcaggcactgttaaaaagcgggctggcagaaacggtgtgcggattcacggtcaataaagtatgtggttcgggtcttaaaagtgtggcgcttgccgcccaggccattcaggcaggtcaggcgcagagcattgtggcggggggtatggaaaatatgagtttagccccctacttactcgatgcaaaagcacgctctggttatcgtcttggagacggacaggtttatgacgtaatcctgcgcgatggcctgatgtgcgccacccatggttatcatatggggattaccgccgaaaacgtggctaaagagtacggaattacccgtgaaatgcaggatgaactggcgctacattcacagcgtaaagcggcagccgcaattgagtccggtgcttttacagccgaaatcgtcccggtaaatgttgtcactcgaaagaaaaccttcgtcttcagtcaagacgagttcccgaaagcgaactcaacggctgaagcgttaggtgcattgcgcccggccttcgataaagcaggaacagtcaccgctgggaacgcgtctggtattaacgacggtgctgccgctctggtgattatggaagaatctgcggcgctggcagcaggccttacccccctggctcgcattaaaagttatgccagcggtggcgtgccccccgcattgatgggtatggggccagtacctgccacgcaaaaagcgttacaactggcggggctgcaactggcggatattgatctcattgaggctaatgaagcatttgctgcacagttccttgccgttgggaaaaacctgggctttgattctgagaaagtgaatgtcaacggcggggccatcgcgctcgggcatcctatcggtgccagtggtgctcgtattctggtcacactattacatgccatgcaggcacgcgataaaacgctggggctggcaacactgtgcattggcggcggtcagggaattgcgatggtgattgaacggttgaattaaaaaaaaaaaccccgcccctgacagggcggggtttttttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z