BBa_K1587005 1 BBa_K1587005 Butyrate synthesis pathway (BBa_1587004) without ccr gene regulated by constitutive promoter p(Bla) 2015-09-13T11:00:00Z 2015-09-16T10:53:43Z We did the assembly of several sequences from ncbi, and hbd and crt genes which came originally from Clostridium acetobutylicum were optimized to work in E.coli. Constitutive promoter p(Bla). 3-hydroxybutyryl-CoA dehydrogenase hbd coding region. 3-hydroxybutyryl-CoA dehydratase crt coding region. Acyl-CoA thioesterase 2 tesB coding region. acetyl-CoA acetyltransferase atoB coding region. Every genes are framed by strong RBS based on Ron Weiss thesis. This operon ends with an artificial terminator according to BBa_B1006. Due to a lack of time we decided to have this construction synthesised instead of using the assembly protocol. This construction without the ccr gene is a negative control of the butyrate production in E. coli. Moreover, it was aimed to be inserted in our regulation system. This construction may be used in order to produce all the intermediates of butyrate up to crotonyl-CoA. false false _2004_ 24598 24595 9 false No information false Louise GODY annotation2461277 1 rbs range2461277 1 912 926 annotation2461275 1 rbs range2461275 1 48 62 annotation2461321 1 atoB range2461321 1 2604 3788 annotation2461286 1 rbs range2461286 1 1713 1727 annotation2461322 1 Terminator range2461322 1 3789 3827 annotation2461276 1 hbd range2461276 1 63 911 annotation2461320 1 rbs range2461320 1 2589 2603 annotation2461318 1 tesB range2461318 1 1728 2588 annotation2461297 1 crt range2461297 1 927 1712 annotation2461274 1 p(Bla) range2461274 1 1 35 BBa_K1587005_sequence 1 tgtaagtttatacataggcgagtactctgttatgggctagcctcgagattaaagaggagaaaatgaagaaagtttgtgttattggcgctggtactatgggctccggtatcgctcaggcattcgctgctaagggcttcgaagtagttctgcgtgatatcaaagacgagtttgtggaccgtggcctggacttcatcaacaaaaacctgtccaagctggttaaaaagggtaagatcgaagaagctaccaaagttgaaatcctgactcgtatctctggcaccgtagacctgaacatggctgcggactgcgacctggttattgaagctgctgtggagcgcatggatattaaaaagcagatctttgcagacctggacaacatttgcaaaccggagaccatcctggcatccaacacttcttccctgagcatcacagaagttgcttcagctactaaacgtccggacaaagttatcggcatgcatttcttcaacccggctccggtaatgaaactggttgaagttatccgtggtattgctacttcccaggaaactttcgacgctgttaaagaaactagcatcgcgatcggcaaagatccggttgaagttgcagaagctccgggtttcgtagttaaccgtattctgatcccgatgatcaacgaagcagttggcatcctggctgaaggtattgcatctgttgaagacatcgacaaagctatgaaactcggcgcgaaccatccgatgggtccgctggaactgggtgacttcatcggtctggacatctgcctggcgatcatggatgtcctgtactccgagaccggcgactctaaataccgcccgcacactctgctgaaaaaatacgtacgcgcaggttggctgggccgtaagtctggtaaaggcttctacgactactctaagtaaattaaagaggagaaaatggaactgaacaatgttatcttagaaaaggaaggcaaagtagctgtggttaccatcaaccgcccgaaagcactgaacgccctgaactctgacaccctgaaagaaatggactacgtgatcggtgaaattgagaacgactctgaagtactggcagtgattctgaccggcgctggtgaaaaaagcttcgtagctggcgctgacatctccgaaatgaaagagatgaacactatcgaaggtcgtaaatttggcatcctgggtaacaaagtgttccgtcgtctggaactgctggaaaagccggttatcgccgcagttaacggcttcgctctgggtggcggctgcgaaatcgcgatgtcctgcgacattcgcatcgcttcctctaacgcacgtttcggtcaaccggaagtaggtctgggcatcaccccgggcttcggtggcacccagcgtctgtcccgtctggttggtatgggcatggctaaacagctgatcttcaccgcgcagaacatcaaggcagacgaagctctgcgtatcggcctggttaacaaagtagttgaaccgtctgaactgatgaacactgctaaagaaatcgctaacaaaatcgtatctaacgcaccggtagcagttaaactgagcaaacaggctatcaaccgtggcatgcagtgcgacatcgacaccgctctggcttttgagtccgaagcgtttggtgaatgcttcagcactgaagaccagaaagacgcgatgactgctttcatcgaaaagcgcaaaatcgaaggcttcaaaaaccgctaaattaaagaggagaaaatgagtcaggcgctaaaaaatttactgacattgttaaatctggaaaaaattgaggaaggactctttcgcggccagagtgaagatttaggtttacgccaggtgtttggcggccaggtcgtgggtcaggccttgtatgctgcaaaagagaccgtccctgaagagcggctggtacattcgtttcacagctactttcttcgccctggcgatagtaagaagccgattatttatgatgtcgaaacgctgcgtgacggtaacagcttcagcgcccgccgggttgctgctattcaaaacggcaaaccgattttttatatgactgcctctttccaggcaccagaagcgggtttcgaacatcaaaaaacaatgccgtccgcgccagcgcctgatggcctcccttcggaaacgcaaatcgcccaatcgctggcgcacctgctgccgccagtgctgaaagataaattcatctgcgatcgtccgctggaagtccgtccggtggagtttcataacccactgaaaggtcacgtcgcagaaccacatcgtcaggtgtggatccgcgcaaatggtagcgtgccggatgacctgcgcgttcatcagtatctgctcggttacgcttctgatcttaacttcctgccggtagctctacagccgcacggcatcggttttctcgaaccggggattcagattgccaccattgaccattccatgtggttccatcgcccgtttaatttgaatgaatggctgctgtatagcgtggagagcacctcggcgtccagcgcacgtggctttgtgcgcggtgagttttatacccaagacggcgtactggttgcctcgaccgttcaggaaggggtgatgcgtaatcacaattaaattaaagaggagaaaatgaaaaattgtgtcatcgtcagtgcggtacgtactgctatcggtagttttaacggttcactcgcttccaccagcgccatcgacctgggggcgacagtaattaaagccgccattgaacgtgcaaaaatcgattcacaacacgttgatgaagtgattatgggtaacgtgttacaagccgggctggggcaaaatccggcgcgtcaggcactgttaaaaagcgggctggcagaaacggtgtgcggattcacggtcaataaagtatgtggttcgggtcttaaaagtgtggcgcttgccgcccaggccattcaggcaggtcaggcgcagagcattgtggcggggggtatggaaaatatgagtttagccccctacttactcgatgcaaaagcacgctctggttatcgtcttggagacggacaggtttatgacgtaatcctgcgcgatggcctgatgtgcgccacccatggttatcatatggggattaccgccgaaaacgtggctaaagagtacggaattacccgtgaaatgcaggatgaactggcgctacattcacagcgtaaagcggcagccgcaattgagtccggtgcttttacagccgaaatcgtcccggtaaatgttgtcactcgaaagaaaaccttcgtcttcagtcaagacgagttcccgaaagcgaactcaacggctgaagcgttaggtgcattgcgcccggccttcgataaagcaggaacagtcaccgctgggaacgcgtctggtattaacgacggtgctgccgctctggtgattatggaagaatctgcggcgctggcagcaggccttacccccctggctcgcattaaaagttatgccagcggtggcgtgccccccgcattgatgggtatggggccagtacctgccacgcaaaaagcgttacaactggcggggctgcaactggcggatattgatctcattgaggctaatgaagcatttgctgcacagttccttgccgttgggaaaaacctgggctttgattctgagaaagtgaatgtcaacggcggggccatcgcgctcgggcatcctatcggtgccagtggtgctcgtattctggtcacactattacatgccatgcaggcacgcgataaaacgctggggctggcaacactgtgcattggcggcggtcagggaattgcgatggtgattgaacggttgaattaaaaaaaaaaaccccgcccctgacagggcggggtttttttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z