BBa_K1590002 1 BBa_K1590002 Human Haptoglobin 2015-09-07T11:00:00Z 2015-09-18T03:45:40Z In silico optimised version for E. coli K12 strains. Sequence ordered through IDT. Haptoglobin is a binding protein with high affinity for free Haemoglobin. false false _2007_ 8083 24715 9 true Choosing the correct codon-bias, remove 'illegal' restriction sites, ensure that (stop)codons are in frame, addition of standard prefix and suffix. Primers for cloning Haptoglobin into overexpression vector pQE80-L. Forward: 5' GCGC GGATCC TCTGCGCTGGGTGCGGTT 3' Reverse: 5' GCGC GGTACC TTAGTTTTCCGCGATGGT 3' Primers for cloning Haptoglobin into vector for bacterial 2-hybrid system, pUT18. Forward: 5' GCGC GGATCC ATGTCTGCGCTGGGTGCG 3' Reverse: 5' GCGC GAATTC GAGTTTTCCGCGATGGTTTTC 3' Primers for cloning Haptoglobin into vector for bacterial 2-hybrid system, pT25. Forward: 5' GCGC GGTACC TTAGTTTTCCGCGATGGT 3' Reverse: 5' GCGC GGATCC ATGAACCTGCTGTCTCCGATCCTGTCT 3' Part was synthesised with appropriate prefix/suffix sequences, hence no separate primers were required for cloning it into pSB1C3. false Manuel Blank annotation2447347 1 ATG start codon range2447347 1 1 3 annotation2447348 1 Human Haptoglobin Protein Coding Sequence range2447348 1 1 1218 annotation2447349 1 TAA stop codon range2447349 1 1219 1221 BBa_K1590002_sequence 1 atgtctgcgctgggtgcggttatcgcgctgctgctgtggggtcagctgttcgcggttgactctggtaacgacgttaccgacatcgcggacgacggttgcccgaaaccgccggaaatcgcgcacggttacgttgaacactctgttcgttaccagtgcaaaaactactacaaactgcgtaccgaaggtgacggtgtttacaccctgaacgacaaaaaacagtggatcaacaaagcagtaggcgacaaactgccggaatgcgaagccgatgacggctgtccgaaaccaccggagatcgctcatggctacgtcgaacactcggtgcgttatcagtgcaaaaactactacaaactgcgcacggaaggagacggggtgtacactcttaataacgagaaacagtggattaacaaagcggttggcgataagctgccggaatgtgaggcagtctgcggtaaaccgaaaaacccggcgaacccggttcagcgtatcttgggcgggcacttagatgcgaaaggttcattcccgtggcaggccaaaatggtttcccaccacaatctcacgacaggggcgaccctgatcaacgagcagtggttgctgaccactgctaaaaatctgtttctgaaccatagcgaaaatgcgacggcgaaagacatcgcgccgaccctgaccctgtacgttggtaaaaaacagctggttgaaatcgaaaaagttgttctgcacccgaactactctcaggttgacatcggtctgatcaaactgaaacagaaagtttctgttaacgaacgtgttatgccgatctgcctgccgtctaaagactacgcggaagttggtcgtgttggttacgtttctggttggggtcgtaacgcgaacttcaaattcaccgaccacctgaaatacgttatgctgccggttgcggaccaggaccagtgcatccgtcactacgaaggttctaccgttccggaaaaaaaaaccccgaaatctccggttggtgttcagccgatcctgaacgaacacaccttctgcgcgggtatgtctaaataccaggaagacacctgctacggtgacgcgggttctgcgttcgcggttcacgacctggaagaagacacctggtacgcgaccggtatcctgtctttcgacaaatcttgcgcggttgcggaatacggtgtttacgttaaagttacctctatccaggactgggttcagaaaaccatcgcggaaaactaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z