BBa_K1590004 1 BBa_K1590004 Regulator or Chromate responsive promoter 2015-09-08T11:00:00Z 2015-09-18T03:51:13Z PCR-amplification from biobrick BBa_K1058008 (pChrBGFP). The protein encoded by this sequence is a putative chromate responsive repressor of pChr (BBa_K1590003). false false _2007_ 8083 24715 9 false The part was partially optimised by altering the first 15 codons by means of a non-specific primer. The codons were biased towards maximum expression level in E. coli K12 strains. Primers used for amplification of ChrB coding-region, while optimising the 15 codons, adding a standard prefix, and a standard suffix to it: Forward: GCGC GAATTCGCGGCCGCTTCTAG A TGAACCTGCTGTCTCCGATCCTGTCTCTGCCGACCGAAAACGCG Reverse: GCGC CTGCAGCGGCCGCTACTAGT ATTATT ATACGGTGAGGGTCCC Primers for amplification of partially optimised ChrB from BBa_K1058008 and adding a standard prefix, a PstI restriction site, and a His6-tag to it. Forward: GCGC GAATTCGCGGCCGCTTCTAG A TGAACCTGCTGTCTCCGATCCTGTCTCTGCCGACCGAAAACGCG Reverse: GCGC CTGCAG TTATTA ATGGTGATGGTGATGGTGTACGGTGAGGGTCCC Primers for amplification of partially optimised ChrB from recombinant plasmid and adding BamHI/PstI restriction sites and a His6-tag to it. Forward: GCGC GGATCC ATGAACCTGCTGTCTCCGATCCTGTCT Reverse: GCGC CTGCAG TTATTA ATGGTGATGGTGATGGTG TACGGTGAGGGTCCC false Manuel Blank annotation2447696 1 ATG start codon range2447696 1 1 3 annotation2447699 1 optimised for K12 range2447699 1 1 15 annotation2447697 1 ChrB coding sequence range2447697 1 1 936 annotation2447698 1 TAA stop codon range2447698 1 937 939 BBa_K1590004_sequence 1 atgaacctgctgtctccgatcctgtctctgccgaccgaaaacgcgaccgtccggcaacggacgtggcgtgccctcaaagcctccggcgctgcggtgctgcgcgacggcgtgtacctgatgcccgaccgcgacgaatgccgcgcggtgctggataacttggcctccgatgtgcgtgaaggcggtggggtcgctcatgtgctgcgtatggaagaccctgaaggggtcaacttcgtcgcattattcgaccgcagcaacgattttgctgccttgctggtcgatgtccatcatctcaggcagacgctgacattggataccgtgcaagacgtgctgcggcaggtgcgtaagcttcgcaaatcctttactacgctggttgaaatcgacttctaccccggcgaggctcagcgtcaagcggacagtgcgttgtgtgaactggaacaagcttgcgcccgcacgctgtcgccggacgagccgcacgctgtagaggggactattacccgcttggatcgtttggattaccaggcccgtacctgggccactcgcgcacgtccttgggttgatcggctcgccagcgcatggctgatccggcgcttcatcgacccgcaggcgcgcatcctttggttggcaacccctgcggactgcccgccggatgcgttgggcttcgacttcgatggcgcgacgttcagccatgtcggcagccgtgtcacgttcgaggtcctggcggcgagctttgggctggaacagcccgccatcacaaggattggccttgtggtgcattacctcgacgtgggcggcatccagccgccagaggccactggtatcgaaagcgtactggccggtttgcgggaaacggttgaccacgacgatcaactattggccatcgcctccactgtgttcgatggcttactggccagctttgaaaaagggaccctcaccgtatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z